Histology I - Mt. SAC 35... · Histology • Study of tissues • Tissues = a group of cells...
Transcript of Histology I - Mt. SAC 35... · Histology • Study of tissues • Tissues = a group of cells...
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Histology IHistology IHistology I
Dr. Carmen E. RexachAnatomy 35
Mt San Antonio College
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Histology• Study of tissues • Tissues = a group of cells
performing a common function
• Microscopic anatomy• Includes how different
tissues are arranged to form organs and other structures
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Extracellular matrix: cell surface
• Composition– Fibrous structural and adhesion proteins
embedded in gelatinous polysaccharide ground substance
• Function– Binds cells together to form tissues
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Extracellular matrix: cell surface
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Cell Junctions• Tight junctions
– Near apical surface• Adhering junctions
– Below tight junctions• Desmosomes
– Sites that stretch• Gap junctions
– Shared cytoplasm
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Tissue sections
Longitudinal sections Transverse or cross section
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Tissue sections
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Primary tissue classes
• Epithelial• Connective
• Differences– Matrix
• variation• Primary constituents
– Fibrous proteins– Ground substance
– Amount of cells vs matrix
• Nervous• Muscular
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Epithelial tissue• Composition
– One or more layers of closely adhering cells– Apical surface and basal surface– Rest on a basement membrane– Avascular– Supported by connective tissues
• Function– Cover organ surfaces– Form secretory tissue– Form ducts of glands
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Classification
• Criteria– Number of cell layers– Morphology of the
surface cells• Two major categories
– Simple (one layer)– Stratified (two or more
layers)
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Cell shape• Squamous
• Cuboidal
• Columnar
• Acinar
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Simple Epithelia
• General– All cells touch basement membrane
• Exception: Pseudostratified columnar
• Categories– Simple squamous– Simple cuboidal– Simple columnar– Pseudostratified columnar
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Simple Epithelia
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Microvilli vs. Cilia
Microvilli are folds in the apical surface of the plasma membrane. They increase cell surface area.
Cilia are composed of microtubules and project from the apical surface. Function: to move things across the cell surface
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Acini
• Acinar cells are often arranged in a circle (acinus, acini), creating structures that resemble flowers or a pie that’s been cut into slices.
• Many exocrine glands are composed of acini.
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Stratified Epithelia• General characteristics
– Up to 20 or more cell layers– Named for shape of surface cells
• Exception: transitional epithelium• Stratified squamous
– Most common– Exfoliation– Keratinized tissue (cornified)– Nonkeratinized
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Stratified epithelia
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Transitional epithelium
Note the “dome cells” at the apical surface
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Connective Tissue
• Less cells, more ECF (ground substance)• Variable, widely distributed, most
abundant• Three categories of mature tissue
– Fibrous CT– Supportive CT (bone, cartilage)– Fluid CT (blood)
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Functions
• Binds organs• Support• Protection
– Physical– Immune
• Movement• Storage• Heat production• Transportation
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Fibrous Connective Tissue: Components
• Cells– Fibroblasts– Macrophage– Leukocytes– Plasma Cells– Mast Cells– Adipocytes
• Fibers – Collagenous– Reticular– Elastic
• Ground substance
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Types of Fibrous CT• Loose
– Lots of ground substance– dissolves in vitro– Types
• Areolar, reticular, adipose• Dense
– Fiber is predominant component– Types
• Dense regular, dense irregular
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Loose CT
Areolar connective tissue
Reticular connective tissue
Adipose connective tissue
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Areolar Connective Tissue• Cells
– All six types• Matrix
– Predominately collagenous– Some elastic/reticular
• General– Very vascular– Loosely organized– Varied– Underlies almost all
epithelial tissue
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Reticular• Reticular fibers and fibroblasts• Stroma of organs and tissues such as
lymph nodes, spleen, thymus, bone marrow
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Adipose Tissue
• Adipocytes most abundant cell type
• Supported by areolarCT, reticular CT, blood vessels
• Function: energy storage
• White fat vs. Brown fat
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Dense Connective Tissue• Dense Regular
– Closely packed parallel collagen fibers + fibroblasts
– Tendons and ligaments
– Yellow elastic tissue• Elastic fibers• Fibroblasts with
larger nuclei– Wavy elastic sheets
in walls of medium/large arteries
• Dense Irregular– Collagen fibers in
random arrangement + fibroblasts
– Dermis, protective capsule around organs, fibrous sheet around bones, etc.
– Similar to areolar, but less “open”space
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Dense Connective Tissue
Dense Regular Dense Irregular
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Cartilage• Flexible matrix• Avascular except when
transforming to bone• External ear, tip of nose,
larynx• Cells
– Chondroblasts• secrete matrix
– Chondrocytes• trapped in lacunae
• Three types– Hyaline– Elastic– Fibrocartilage
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Hyaline Cartilage• Matrix
– smooth, frictionless – mix of collagen, elastin,
chondroitin (a glucosaminoglycan)
• Cells– Cell nests
• Usually covered by perichondrium– Dense irregular CT covering– inner layer produces new
chondrocytes• Examples:
– Costal cartilage– Nasal septum– C-ring cartilage of trachea– Fetal skeleton
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Elastic Cartilage
• Matrix– Web-like mesh of
elastic fibers– Appearance of fur
• Perichondriumalways present
• Examples:– Pinna– Epiglottis
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Fibrocartilage• Characteristics
– Parallel collagen fibers
– Rows of chondrocytes in lacunae
– No perichondrium• Examples
– Pubic symphysis– Intervertebral
discs– Menisci of the
synovial joints
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Bone• Two forms
– Spongy• Heads of long
bone• Named for
appearance– Compact
• No visible spaces• Dense calcified
tissue• External surface
of all bone
• Structure of compact bone– Haversian canal– Osteon– Lamellae– Lacunae– Canaliculi
• Periosteum
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Compact Bone
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Blood• Plasma + formed
elements• Erythrocytes• Leukocytes
– Neutrophils– Basophils– Eosinophils– Monocytes– Lymphocytes
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Hemopoiesisformation of formed elements
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Nervous Tissue
• Excitable tissue• Function
– Rapid communication
• Cell types– Neurons– Neuroglia
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Neurons
• impulse: an all or none electrical event
• neurotransmitters: chemical messengers released by neurons
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Neuron
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Neuroglia (nerve glue)
• 6 subtypes• highly branched
like neurons• not capable of
impulses• perform supportive
(connective tissue type) functions
astrocyte
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Nerves: Cross section
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Muscular Tissue• Contracts when stimulated• Three types
– Skeletal• Striated, multinucleated, voluntary• Muscle fibers
– Cardiac• Striated, uninucleate, involuntary• Short branching cells• Intercalated discs
– gap junctions– desmosomes
– Smooth• No striations, uninucleate, involuntary• Short fusiform cells• Visceral muscle
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Muscular Tissue
Skeletal Muscle
Cardiac muscle
Smooth muscle