For pathway key and background, please visit:

www.cst-science.com/hippo

Hippo Signaling

SCFβTrCP

AMOT

Hippo Signaling

RT

K

RT

K

Crb YAP/TAZYAP/TAZ YAP/TAZ

YAP/TAZ

AMOTCrb

AMOT

AMOT

PTPN14

PTPN14

FRMD6

FRMD6

DestructionComplex

aPKC

α-Cateninα-Catenin

14-3-314-3-3

14-3-3

Ajuba/LIM

YAP/TAZ

ON-State (YAP/TAZ Inactive)OFF-State (YAP/TAZ Active)

Cytoplasm

Nucleus Cytoplasm

Nucleus

Target Gene Transcription

YAP/TAZ YAP/TAZ YAP/TAZ

YAP/TAZ

YAP/TAZYAP/TAZ

YAP/TAZ

LATS1/2

MST1/2

YAP/TAZ

YAP/TAZ YAP/TAZ

YAP/TAZYAP/TAZ

TEADs ERBB4 EGR-1

MOB1SAV1

MAPK

AMPK TAO1-3

MAPK4Ks

Rho

Rho

TBX5 SMADs RUNXTEADs ERBB4

PRP4K

EGR-1 TBX5 SMADs RUNX

GPCRSignaling

Growth FactorSignaling

ECM GPCRSignaling

Integrins

ECM/MechanicalSignaling

Integrins

ZO-2

Adhe

rens

Jun

ctio

nTi

ght J

unct

ion

ZO-2

Hippo Kinase Core

LATS1/2

MST1/2

MOB1

SAV1

Hippo Kinase Core

Ajuba/LIM

Adhe

rens

Jun

ctio

nTi

ght J

unct

ion

NF2NF2

YES1

ABL

NF2NF2

Ras PI3K

PDK1 MARK4 RASSF

STRIPAK

SLMAP

STRIPAK

SLMAP

KIBRA KIBRA

SRC

SAV1NF2

PAR

CK1

Gα12/13

Gαq/11

Gαi/o

ActinCytoskeleton

GαS

YAP/TAZSequestration

YAP/TAZSequestration

YAP/TAZDegradation

ActinCytoskeleton

rev. 02/14/20

No ContactInhibition

ContactInhibition

For R

esea

rch

Use

Only

. Not

For

Use

In D

iagn

ostic

Pro

cedu

res.

19-

CEP-

2995

3-PW

H

© 2020 Cell Signaling Technology, Inc. All trademarks are the property of their respective owners. U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom. For the most up-to-date trademark information, please visit www.cellsignal.com/trademarks

For pathway key and background, please visit:

www.cst-science.com/hippo

Key Products for Hippo SignalingVisit www.cellsignal.com for a full listing of antibodies, related kits, and reagents.

ANTIBODIES APPLICATIONS REACTIVITY

34648 Ajuba (D4D8P) Rabbit mAb WB, IP H, Mk

43130 Angiomotin (D2O4H) Rabbit mAb WB, IP, IF-IC H

14688 FRMD6 (D8X3R) Rabbit mAb WB, IP H, M, R

8774 KIBRA Antibody WB, IP H, Mk

3477 LATS1 (C66B5) Rabbit mAb WB, IP H, M, Mk

8654 Phospho-LATS1 (Thr1079) (D57D3) Rabbit mAb

WB H, M

9153 LATS1 Antibody WB H, Mk

9157 Phospho-LATS1 (Ser909) Antibody WB H

5888 LATS2 (D83D6) Rabbit mAb WB H

13245 LIMD1 Antibody WB H, M

92427 MAP4K3 (D1L4G) Rabbit mAb WB, IP H, Mk

6995 Merlin (D1D8) Rabbit mAb WB, IP H, M, R

12888 Merlin (D3S3W) Rabbit mAb WB, IP, IF-IC H, M, R, Hm, Mk

12896 Merlin (D6N8H) Rabbit mAb WB, IP H, M, R, Hm, Mk

13730 MOB1 (E1N9D) Rabbit mAb WB, IP H, M, R, Hm, Mk

8843 Phospho-MOB1 (Thr12) (D2E3) Rabbit mAb

WB H, M, R, Mk

8699 Phospho-MOB1 (Thr35) (D2F10) Rabbit mAb

WB, IHC-P H, M, R, Mk

3863 MOB1 Antibody WB H, M, R

14946 MST1 (D8B9Q) Rabbit mAb WB, IP H, M, R

49332 Phospho-MST1 (Thr183)/MST2 (Thr180) (E7U1D) Rabbit mAb

WB H, M

3952 MST2 Antibody WB, IP H, M, R, Mk, B

8577 PRP4K (D27A1) Rabbit mAb WB, IP H, R, Mk

13808 PTPN14 (D5T6Y) Rabbit mAb WB H, M, R

86026 RASSF1 (E7T6O) Rabbit mAb WB H

2117 RhoA (67B9) Rabbit mAb WB H, M, R, Mk, B

13301 SAV1 (D6M6X) Rabbit mAb WB, IP, IF-IC H, M

3507 Sav1 Antibody WB, IP H, R, Mk

70148 TAZ (D3I6D) Rabbit mAb WB, IP, ChIP, ChIP-seq H, M

83669 TAZ (E8E9G) Rabbit mAb WB, IP, IF-IC, ChIP H, M, R, Mk

59971 Phospho-TAZ (Ser89) (E1X9C) Rabbit mAb WB, IP, IHC-P H, M, R

71192 TAZ (E5P2N) Mouse mAb WB, IP H, M, R, Mk

ANTIBODIES APPLICATIONS REACTIVITY

4883 TAZ (V386) Antibody WB, ChIP H, M, R

72804 TAZ (E9J5A) XP® Rabbit mAb WB, IHC-Bond, IHC-P H, M, Mk

13295 Pan-TEAD (D3F7L) Rabbit mAb WB, IP H, M, R, Mk

12292 TEAD1 (D9X2L) Rabbit mAb WB, IP, IHC-P, IF-IC H, M, Mk

13224 TEAD3 Antibody WB, IP H, M, R

11984 ß-TrCP (D12C8) Rabbit mAb WB H, M, R, Mk

11831 WBP2 Antibody WB H, M, R, Mk

14074 YAP (D8H1X) XP® Rabbit mAb WB, IP, IHC-P, IF-IC, F, ChIP, ChIP-seq

H, M, R, Hm, Mk

53749 Phospho-YAP (Ser109) (E5I9G) Rabbit mAb

WB, IP H, M, R, Mk

13008 Phospho-YAP (Ser127) (D9W2I) Rabbit mAb

WB, IP, IHC-P H, M, R

13619 Phospho-YAP (Ser397) (D1E7Y) Rabbit mAb

WB, IP H, M, R

12395 YAP (1A12) Mouse mAb WB, IP, IHC-P H, M, R, Hm, Mk

4912 YAP Antibody WB, IP H, M, R, Mk

4911 Phospho-YAP (Ser127) Antibody WB H, M, R

46931 Phospho-YAP (Ser109) Antibody WB, IP H, M, R, Mk

29495 Non-phospho (Active) YAP (Ser127) (E6U8Z) Rabbit mAb

WB H

8418 YAP/TAZ (D24E4) Rabbit mAb WB, IP, IHC-P H, M, Mk

KITS

56612 Hippo Pathway: Upstream Signaling Antibody Sampler Kit

8579 Hippo Signaling Antibody Sampler Kit

48804 PathScan® Phospho-YAP (Ser127) Sandwich ELISA Kit

ELISA H

57046 PathScan® Phospho-YAP (Ser397) Sandwich ELISA Kit

ELISA H

32939 PathScan® Total YAP Sandwich ELISA Kit

ELISA H, M

52420 Phospho-YAP/TAZ Antibody Sampler Kit

56674 YAP/TAZ Transcriptional Targets Antibody Sampler Kit

TAZ (E9J5A) XP® Rabbit mAb #72804: IHC analysis of paraffin-embedded human breast carcinoma using #72804.

kDa

YAP

β-Actin

140

10080

60504030

20

10

5040

HeLa

– + YAP knock-out

200

YAP (D8H1X) XP® Rabbit mAb #14074: WB analysis of HeLa cell extracts, untreated (-) or YAP knock-out (+), using #14074.

C

YAP (D8H1X) XP® Rabbit mAb #14074: Confocal IF analysis of low confluence MCF 10A (A), high confluence MCF 10A (B), and YAP negative RL-7 (C) cells using #14074 (green). Blue pseudocolor in lower images = DRAQ5® #4084 (fluorescent DNA dye). Increased nuclear localization of YAP protein is seen in low confluence (proliferating) cells.

BA

TAZ (E9J5A) XP® Rabbit mAb #72804: IHC analysis of paraffin-embedded human colon carcinoma using #72804.