High Performance Liquid Chromatography

High Performance Liquid ChromatographyGiven By : Syed Murtaza Shah Roll no : 16CH48

SUBTOPICS :Chromatography DefinitionHistory of ChromatographyWorking Principle of ChromatographyTypes of ChromatographyHigh Performance Liquid Chromatography(HPLC)Working, Parts and Applications of HPLC SYSTEM

How does it Works??

Simple Experiment to understand Chromatography

History of Chromatography

Experiment of M.S Tswett

Basic Termologies used in ChromatographyTermDefinitionMobile phase or carriersolvent moving through the columnStationary phase or adsorbentsubstance that stays fixed inside the columnEluentfluid entering the columnEluatefluid exiting the column (that is collected in flasks)Elutionthe process of washing out a compound through a column using a suitable solventAnalytemixture whose individual components have to be separated and analyzed

Working Principle of Chromatography

Chromatography works on Adsorption Principle

Types of Chromatography

Paper Chromatography

Gas Chromatography

Column Chromatography

High Performance Liquid Chromatography

Parts of Hplc System

Working of HPLC system

How does the User knows what and how many types of Elements collected form sample

Scales of HPLC

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