High content imaging with the yokogawa cv7000 system
-
Upload
david-lorenz -
Category
Health & Medicine
-
view
228 -
download
0
Transcript of High content imaging with the yokogawa cv7000 system
![Page 1: High content imaging with the yokogawa cv7000 system](https://reader034.fdocuments.net/reader034/viewer/2022051516/55a46c371a28ab0f778b45cb/html5/thumbnails/1.jpg)
Yokogawa Cell Voyager 7000
Wako Automation Introduction and Overview
![Page 2: High content imaging with the yokogawa cv7000 system](https://reader034.fdocuments.net/reader034/viewer/2022051516/55a46c371a28ab0f778b45cb/html5/thumbnails/2.jpg)
Wako Automation
Background
![Page 3: High content imaging with the yokogawa cv7000 system](https://reader034.fdocuments.net/reader034/viewer/2022051516/55a46c371a28ab0f778b45cb/html5/thumbnails/3.jpg)
Wako Pure Chemicals Japan, USA, Germany
!
Wako Diagnostics Japan, USA, Germany
!
Wako Automation USA
![Page 4: High content imaging with the yokogawa cv7000 system](https://reader034.fdocuments.net/reader034/viewer/2022051516/55a46c371a28ab0f778b45cb/html5/thumbnails/4.jpg)
High Content Imaging Platform
Yokogawa CV7000
![Page 5: High content imaging with the yokogawa cv7000 system](https://reader034.fdocuments.net/reader034/viewer/2022051516/55a46c371a28ab0f778b45cb/html5/thumbnails/5.jpg)
High Content Imaging Platform
Automation-Ready
![Page 6: High content imaging with the yokogawa cv7000 system](https://reader034.fdocuments.net/reader034/viewer/2022051516/55a46c371a28ab0f778b45cb/html5/thumbnails/6.jpg)
Yokogawa CSU-W1
•Minimum Bleaching and Toxicity •Reduced Crosstalk •Visualize Dynamic Events in Live Cells
Dual Spinning Disk Systems
![Page 7: High content imaging with the yokogawa cv7000 system](https://reader034.fdocuments.net/reader034/viewer/2022051516/55a46c371a28ab0f778b45cb/html5/thumbnails/7.jpg)
4X Cells Imaged Reduced Crosstalk Thicker Samples
CSU-W1- Wide View Dual Spinning Disk
![Page 8: High content imaging with the yokogawa cv7000 system](https://reader034.fdocuments.net/reader034/viewer/2022051516/55a46c371a28ab0f778b45cb/html5/thumbnails/8.jpg)
Live Cell Imaging
HeLa cell with Azami green, GFP, 10X, 10% 488nm laser 3 days
![Page 9: High content imaging with the yokogawa cv7000 system](https://reader034.fdocuments.net/reader034/viewer/2022051516/55a46c371a28ab0f778b45cb/html5/thumbnails/9.jpg)
Yokogawa CSU-X1 Dual Spinning Disk
Live Cell Imaging
Live Cells imaged every 20 minutes for 24 hours
![Page 10: High content imaging with the yokogawa cv7000 system](https://reader034.fdocuments.net/reader034/viewer/2022051516/55a46c371a28ab0f778b45cb/html5/thumbnails/10.jpg)
Cell Voyager 7000 Configuration
![Page 11: High content imaging with the yokogawa cv7000 system](https://reader034.fdocuments.net/reader034/viewer/2022051516/55a46c371a28ab0f778b45cb/html5/thumbnails/11.jpg)
Simultaneous Imaging with Three (3) Cameras
sCMOS -20˚C 5.5MP, 2560 x 2160 6.5µm pixel
![Page 12: High content imaging with the yokogawa cv7000 system](https://reader034.fdocuments.net/reader034/viewer/2022051516/55a46c371a28ab0f778b45cb/html5/thumbnails/12.jpg)
Objective Choices
![Page 13: High content imaging with the yokogawa cv7000 system](https://reader034.fdocuments.net/reader034/viewer/2022051516/55a46c371a28ab0f778b45cb/html5/thumbnails/13.jpg)
Choose Up To 6 Lenses Dry: 2x, 4x NA .16, 10x NA .4, 20x NA .75,
40x NA .95, 20x NA .45 LWD !
Water Immersion: 40x NA 1.15, 60x NA 1.2
Phase Contrast: 10X, 20X
![Page 14: High content imaging with the yokogawa cv7000 system](https://reader034.fdocuments.net/reader034/viewer/2022051516/55a46c371a28ab0f778b45cb/html5/thumbnails/14.jpg)
Lens Field of View 2x 8.3 mm x7mm 4x 4.15 mm x 3.5 mm 10x 1.66 mm x1.4 mm 20x 0.83 mm x0.7 mm 40x 0.415 mm x0.35 mm 60x 0.276 mm x0.23 mm
sCMOS Camera FOV
![Page 15: High content imaging with the yokogawa cv7000 system](https://reader034.fdocuments.net/reader034/viewer/2022051516/55a46c371a28ab0f778b45cb/html5/thumbnails/15.jpg)
Plate TypeWell Size Lens
96 well 6.5mm 2x objective
384 well 3.25mm 4x objective
1536 well 1.5mm 10x objective
sCMOS Camera FOV
![Page 16: High content imaging with the yokogawa cv7000 system](https://reader034.fdocuments.net/reader034/viewer/2022051516/55a46c371a28ab0f778b45cb/html5/thumbnails/16.jpg)
HeLa cell mitochondria stained with MitoTracker® Red CMXRos 0.2 um steps, 21 slices, 60X water immersion lens.
![Page 17: High content imaging with the yokogawa cv7000 system](https://reader034.fdocuments.net/reader034/viewer/2022051516/55a46c371a28ab0f778b45cb/html5/thumbnails/17.jpg)
Live Cell Imaging
!40X lens, Laser; 561 nm, Laser power; 10%, Exposure time; 20ms, 3 days, 20 min.
Neural Progenitor Cells
![Page 18: High content imaging with the yokogawa cv7000 system](https://reader034.fdocuments.net/reader034/viewer/2022051516/55a46c371a28ab0f778b45cb/html5/thumbnails/18.jpg)
Reproducibility of stage Plate removed 6X
Imaged with 60x Water
![Page 19: High content imaging with the yokogawa cv7000 system](https://reader034.fdocuments.net/reader034/viewer/2022051516/55a46c371a28ab0f778b45cb/html5/thumbnails/19.jpg)
Imaging Modes
Confocal Epifluorescence Phase Contrast Bright Field
![Page 20: High content imaging with the yokogawa cv7000 system](https://reader034.fdocuments.net/reader034/viewer/2022051516/55a46c371a28ab0f778b45cb/html5/thumbnails/20.jpg)
Lasers: Choice of Four
405, 488, 532, 561, 640 !
UV LED: 365 (not confocal)
Halogen Lamp (not confocal)
![Page 21: High content imaging with the yokogawa cv7000 system](https://reader034.fdocuments.net/reader034/viewer/2022051516/55a46c371a28ab0f778b45cb/html5/thumbnails/21.jpg)
Neurite Imaging60X W lens, NA 1.2
![Page 22: High content imaging with the yokogawa cv7000 system](https://reader034.fdocuments.net/reader034/viewer/2022051516/55a46c371a28ab0f778b45cb/html5/thumbnails/22.jpg)
CV7000 Capabilities
![Page 23: High content imaging with the yokogawa cv7000 system](https://reader034.fdocuments.net/reader034/viewer/2022051516/55a46c371a28ab0f778b45cb/html5/thumbnails/23.jpg)
6 to 1536 well plates Microscope Slides
!
Incubated Stage 35˚C - 40˚C, CO2, Humidity
Live Cell Imaging !
Laser Based Autofocus Image Based Autofocus
!
Dispense Option single channel
for kinetics assays
![Page 24: High content imaging with the yokogawa cv7000 system](https://reader034.fdocuments.net/reader034/viewer/2022051516/55a46c371a28ab0f778b45cb/html5/thumbnails/24.jpg)
Image Standard Plate Types & Microscope Slides
![Page 25: High content imaging with the yokogawa cv7000 system](https://reader034.fdocuments.net/reader034/viewer/2022051516/55a46c371a28ab0f778b45cb/html5/thumbnails/25.jpg)
!
!
!
3 colors, 96 wells, <2 min3 colors, 384 wells, <5 min3 colors, 1536 wells, <18 min
(50ms, 1 field, 1 z Stack)
Imaging Speed (3 cameras)
Exceptional Image Quality
![Page 26: High content imaging with the yokogawa cv7000 system](https://reader034.fdocuments.net/reader034/viewer/2022051516/55a46c371a28ab0f778b45cb/html5/thumbnails/26.jpg)
Additional Features
![Page 27: High content imaging with the yokogawa cv7000 system](https://reader034.fdocuments.net/reader034/viewer/2022051516/55a46c371a28ab0f778b45cb/html5/thumbnails/27.jpg)
Thick Sample Imaging Possible
with CSU-W1
![Page 28: High content imaging with the yokogawa cv7000 system](https://reader034.fdocuments.net/reader034/viewer/2022051516/55a46c371a28ab0f778b45cb/html5/thumbnails/28.jpg)
Cy2 Collagen IV (Basement membrane) - green CY3 Pgp9.5 (pan-neuronal Marker)- red
DAPI DNA (Cell nucleuc)- Blue
Exp: 200ms, Z axis 80um , 1.0µm spacing, 81 slices in total Objective: 60X
![Page 29: High content imaging with the yokogawa cv7000 system](https://reader034.fdocuments.net/reader034/viewer/2022051516/55a46c371a28ab0f778b45cb/html5/thumbnails/29.jpg)
The CV7000 Benefits
![Page 30: High content imaging with the yokogawa cv7000 system](https://reader034.fdocuments.net/reader034/viewer/2022051516/55a46c371a28ab0f778b45cb/html5/thumbnails/30.jpg)
Laser light focused through microlens Reduced photo-‐bleaching and photo-‐toxicity
Be:er confocal images
Yokogawa Dual Spinning Disk System
![Page 31: High content imaging with the yokogawa cv7000 system](https://reader034.fdocuments.net/reader034/viewer/2022051516/55a46c371a28ab0f778b45cb/html5/thumbnails/31.jpg)
unmatched speed
4x field of view
live cell imaging for days