Health Sciences Core Research Facilities

Genomics Research Core

Gene Expression-Immune Profiling-CNV-ATAC

High cell viability will result in:▪ Greater recovery of cells

▪ High fraction of reads in cells

▪ Cleaner data with lower background

▪ Gentle handling

▪ Buffers-PBS/0.04% BSA preferred

▪ Timing-Cells should be delivered quickly after preparation

▪ We offer dead cell removal and filtering

▪ The GRC is available to offer guidance and support along the way▪ Different cell types may have unique requirements

▪ We offer multiple QC opportunities for optimizing cell preparation

▪ We also have a Miltenyi GentleMACS Dissociator

Janette Lamb, Ph.D.

Director

jal18@pitt.edu

Deborah Hollingshead, MS

Assistant Director

hollings@pitt.edu

Heidi Monroe

Single Cell Sequencing

Next Generation Sequencing

hmm10@pitt.edu

Erica Fong

Single Cell Sequencing

esf14@pitt.edu

Bryan Thompson

Next Generation Sequencing

brt2@pitt.edu