Zach Bush, MD

Internal Medicine, Endocrinology and Metabolism

Founder, Director of Clinical Affairs

Revolution Health Center

Charlottesville, VA

Founder, CEO

Biomic Sciences

Gut Microbiome and Gut Permeability, the Zero-Point for the

Disease Epidemics of the Developed World

Gut Microbiome and Gut Permeability, the

Zero-Point for the Disease Epidemics of the

Developed World

Disclosure

• Founder and CEO of Biomic Sciences

• I am the founder and President of Revolution Health

Center, Charlottesville, VA

• My clinical practice, basic science operations,

discoveries, and experience that I share with you today

produce all of my income

• I am not paid by any third-party companies or

organizations for my presentations or educational efforts

US Ranks 49th in Health Outcomes

1st World Epidemics

• Autism 1:45

• Attention Deficit 1:10 (70% medicated)

• Asthma 1:10

• Allergy 1:4

• Diabetes 1:4

• Obesity 1:3

• Major Depression 1:2

• Cancer 1:2

• Dementia 1:1

1st World Epidemics –

With Gut/Brain Source• Autism 1:45

• Attention Deficit 1:10 (70% are medicated)

• Asthma 1:10

• Allergy 1:4

• Diabetes 1:4

• Obesity 1:3

• Major Depression 1:3

• Cancer 1:2

• Dementia 1:1

Root Cause of Disease?Root Cause of Aging and Disease

Inflammation is the root of all chronic disorder

and disease

Autism

Depression

Oxidative Stress

• Acute inflammation can save our lives

– Injury Repair

– Infection control

• Chronic inflammation

– Oxidative stress = positive charge/acid

– Shutdown/overwhelm of the antioxidant system

chronic inflammation = loss of communication

Root Cause of Inflammation?

How has the global population lost cellular

communication over the last 40 years?Root Cause of Aging and Disease

What has happened to drive our

chronic inflammation/disease epidemic

AUTISM - USA CANCER - USA

War on the Microbiome

Antibiotic Usage in Meat Production

• http://ccst.us/images/spotlight/2015/ab300.jpg

Antibiotic Usage and Cancer Rates

• http://ccst.us/images/spotlight/2015/ab300.jpg

Gut Microbiome and Cancer

The geographical variations

. There is also strong evidence for a direct

role for œstrogens in the ætiology of breast cancer. We have already postulated that

.

Since the amount of biliary steroid found in fæces is correlated with the amount of fat

in the diet, this could explain the relation between the amount of dietary fat and the

incidence of breast cancer.

Gut Microbiome and Cancer

In a

The relative abundances of these two bacterial species were

inversely correlated in paired normal breast tissue but not in tumor tissue, indicating that dysbiosis is

associated with breast cancer. Furthermore,

Interestingly,

a finding that could have broad implications in

diagnosis and staging of breast cancer.

Epigenetics / Micro RNA

• Which genes turn on/off is determined by your gut environment

• Micro RNA are translational products of non-coding sequences of the DNA and regulate gene transcription pathways at the DNA level

• 10-15% of the miRNA that is circulating in your blood stream today are from the bacteria in your gut

– Bacterial genome is turning on and off the human genes

• 10-15% of the miRNA in your blood is from fungi

• 1-5% of the miRNA is from the food you eat (meat, soy proteins etc.)

Glyphosate

• N-(phosphonomethyl)glycine

Glyphosate as an antibiotic

• Glyphosate inhibits the activity of the enzyme 5-

enolpyruvyl shikimate 3-phosphate synthase (EPSPS)

• EPSPS

• This metabolic pathway exists in plants, fungi, and

bacteria, but not in animals (Kishore & Shah 1988).

Patents on Glyphosate

Inventor

s:Abraham; William (Wildwood, MO)

Assigne

e:

Monsanto Technology LLC (St. Louis, MO)

Family

ID:32096060

Appl.

No.:10/652,684

Filed: August 29, 2003

Glyphosate formulations and their use for the inhibition of 5-enolpyruvylshikimate-3-phosphate synthase

Abstract

Protozoan parasites of the phylum Apicomplexa include some of the most important causative agents of human and animal disease

Glyphosate formulations and their use for the inhibition of 5-enolpyruvylshikimate-3-phosphate synthase

Abstract

Protozoan parasites of the phylum Apicomplexa include some of the most important causative agents of human and animal diseases, in particular, malaria. The discovery that

Glyphosate Usage World Wide

Globally, glyphosate use has risen

almost genetically engineered glyphosate-tolerant crops were

introduced in 1996.

. The corresponding share globally is 72 %.

Cancer Mortality by state before and after the

1996 Round Up-Resistant Crop Introduction

1970-1994 2007-2011

Glyphosate Usage and Cancer Mortality

2007-2011

Mississippi River Tributaries

Glyphosate, the #1 Antibiotic

GLYPHOSATE

>1.4 Billion pounds/year

Bacterial metabolites:

Carbon-based Redox system• Each bacterial species (30,000 species) creates a unique

subset of carbon metabolites during the digestive process

• These function as an interspecies molecular communication network that allows for coordinated cell protection, injury response, and repair

Carbon Redox

Shilijit – Raw

macrominerals –

heavy metal toxicity

common

Humic Acid – Does not

cross cell membranes

Fulvic Acid – Renal

toxicity

Trace minerals

Terrahydrite – No renal

or hepatic cell toxicity.

Direct Mitochodrial

ROS reduction

First in class to affect

membrane

permeability

What are your bacteria saying?

• Dysbiosis: broken ecosystem– Monoculture

Antibiotics, pesticides, chemicals, processed foods• Weeds grow up: E.Coli, Klebsiela, Psudomonas, Candida

Probiotics – 3 - 24 species, $30 Billion monoculture?

Monoculture = limited vocabulary = limited defense

• Optimal bowel ecology = 20,000 species

Biodiversity = fluid communication = strong defenes

Mining for bacterial communication

Shilijit – Raw macrominerals – heavy metal toxicity common

Humic Acid – Does not cross cell membranes

Fulvic Acid – Renal toxicity

• Trace minerals

Terrahydrite – No renal or hepatic cell toxicity

First-in-class to:

• Directly reduce mitochondrial pro-inflammatory ROS production in healthy cells

• Directly support the extracellular matrix and reduce tight junction permeability

• Expand the microflora through species to species communication

The GALT

• Gastrointestinal-Associated Lymphoid Tissue

GALT: 80% of the

antibodies in your

body are made here

Gut Inflammation

Food Sensitivity

Asthma/Eczema

Allergy

Autoimmune

Diabetes

Cancer

Intercellular Tight Junctions

Firewalls of Defense

Functional Gut Barrier

Gliadin (Gluten)

Tight Junction ImagingCONTROL Gliadin in 1 slice of pizza

Glyphosate

Tight Junction ImagingCONTROL GLYPHOSATE 10 PPM

Bacterial communication impacts

tight junction function

TE

ER

oh

ms/c

m2

Terrahydrite

+

Glyphosate

Terrahydrite

+

Gliadin

Gliadin

(Gluten)Glyphosate

CONTROL GLIADIN

GLIADIN and TerrahydriteTerrahydrite

CONTROL GLYPHOSATE

GLYPHOSATE and TerrahydriteTerrahydrite

Human Intestinal Epithelial Membrane, Zo1 Protein IHC

Terrahydrite Induces

Differential Mitochondrial ROS Activity

In Healthy vs Cancer Cells

RO

S p

roduction

ROS assay RT- PCR

1 3 5 10 60

Minutes

MCF-7

PRTC

GLUTENToxic Peptides

CXCR3

InflammationZONULIN

ENZ

TJ breakdown

Leaky Gut/Brain Leak GutGLYPHOSATE

Dysbiosis/Yeast

Overgrowth

DPP4 Enzymes

Chronic Inflammation

Leaky Gut/Brain

Terrahydrite

Leaky Gut - Leaky Brain

Inflammation Cascade from birth to death

Colic

Sensory-Processing Defects

Attention Deficit

Anxiety Disorders

Major Depression

Disordered Sleep

Insulin Resistance

Infertility

Diabetes

Vascular Disease

Cancer

Dementia

References1. Lerne, A. Matthias, T. (2015) Changes in intestinal tight junction permeability

associated with industrial food additives explain the rising incidence of autoimmune disease. Autoimmunity Reviews, 14, (6), Pages 479-489

2. Jiang W. (2015) Dysbiosis gut microbiota associated with inflammation and impaired mucosal immune function in intestine of humans with non-alcoholic fatty liver disease

3. Scientific Reports 5, Article number: 8096

4. Van Dorpe, S; et al. (2012). "Brainpeps: The blood–brain barrier peptide database". Brain structure & function. 217 (3): 687–718

5. Centers for Disease Control and Prevention (CDC), Key Findings: Trends in the Parent-Report of Health Care Provider-Diagnosis and Medication Treatment for ADHD: United States, 2003-2011 the National Survey of Children's Health (NSCH)

6. Ellwood P, Williams H, Aït-Khaled N, Björkstén B, Robertson C, ISAAC Phase III Study Group. Translation of questions: The International Study of Asthma and Allergies in Childhood (ISAAC) experience. Int J Tuberc Lung Dis. September 2009; 13(9): 1174-1182

7. Massey JT, Moore TF, Parsons VL, Tadros W. Design and estimation for the National Health Interview Survey, 1985–1994. National Center for Health Statistics. Vital Health Stat 1989;2(110)

8. Botman SL, Moore TF, Moriarity CL, Parsons VL. Design and estimation for the National Health Interview Survey, 1995–2004. National Center for Health Statistics.

References6. Janeway, CA Jr.; et al. (2001). "The mucosal immune system". Immunobiology. New

York: Garland Science. 10-13

7. Salminen S, Bouley C, Boutron-Ruault MC, et al. (1998). "Functional food science and gastrointestinal physiology and function". British Journal of Nutrition 80 (S1): S147–S171

8. Moriame G; et al. Viral Suppression and Immune Restoration in the Gastrointestinal Mucosa of HIV Type 1-Infected Patients Initiating Therapy during Primary or Chronic Infection Journal of Virology, August 2006, p. 8236-8247, Vol. 80, No. 16

9. Abbas A.B.; Lichtman A.H. (2009). "Ch.2 Innate Immunity". In Saunders (Elsevier). Basic Immunology. Functions and disorders of the immune system (3rd ed.)

10. Eming, S. A.; Krieg, T.; Davidson, J. M. (2007). "Inflammation in wound repair: molecular and cellular mechanisms". Journal of Investigative Dermatology 127 (3): 514–525

11. Van Dorpe, S; et al. (2012). "Brainpeps: The blood–brain barrier peptide database". Brain structure & function 217 (3): 687–718

12. Schneider, Stefan W.; et al. (March 2004). "Glioblastoma cells release factors that disrupt blood–brain barrier features". Acta Neuropathologica 107 (3): 272–276

References13. H Chen; EE Konofagou. Journal of Cerebral Blood Flow &

Metabolism (2014) 34, 1197–1204

14. H Matsui ; et al. (2011). The pathophysiology of non-steroidal anti-inflammatory drug (NSAID)-induced mucosal injuries in stomach and small intestine J Clin Biochem Nutr. 2011 Mar; 48(2): 107–111

15. Eadon MT; et al. Endotoxemia alters tight junction gene and protein expression in the kidney (2012). Am J Physiol Renal Physiol. 2012 Sep 15; 303(6): F821–F830