Affinity purification of sequence-specific DNA binding proteins
GLUTHATIONE PURIFICATION Low Pressure Get the Best Winter ... · AFFINITY CHROMATOGRAPHY AFFINITY...
Transcript of GLUTHATIONE PURIFICATION Low Pressure Get the Best Winter ... · AFFINITY CHROMATOGRAPHY AFFINITY...
[email protected] www.abtbeads.com
S I Z E E X C L U S I O N C H R O M A T O G R A P H Y
A F F I N I T Y C H R O M A T O G R A P H Y A F F I N I T Y C O U P L I N G
E L E C T R O P H O R E S I S R E A G E N T S A C C E S O R I E S
Glutathione Agarose resins for use in affinity purification of Glutathione-S-transferase (GST) and GST tagged fusion proteins.
• For all kind of proteins: small, large protein complex low expression rate proteins.
TECHNICAL SPECIFICATIONS
BEAD GEOMETRY Spherical, Standard: ~50-150 µm
CROSSLINKED No
AGAROSE % 4%
LIGAND Glutathione, linked via sulphur atom
PRODUCT GLUTATHIONE Agarose Resin
CAT No. 4B-GLU-X
BINDING CAPACITY > 8 mg recombinant GST /ml gel
ANTIMICROBIAL AGENT 20% Ethanol
STORAGE TEMPERATURE 4 - 8º C
• Easy changeover: no need for optimization or protocol change.
• Great adaptability: Bulk format suitable for batch, gravity, peristaltic pump & syringe.
• High purity achieved in one purification step, comparable to market standards.
Low PressureGLUTATHIONE AGAROSE BEADS Resins
Glutathione Agarose resins for use in affinity purification of Glutathione-S-transferase (GST) and GST tagged fusion proteins.
TECHNICAL SPECIFICATIONS
BEAD GEOMETRY Spherical, Standard: ~50-150 µm
CROSSLINKED No
AGAROSE % 4%
LIGAND Glutathione, linked via sulphur atom
PRODUCT GLUTATHIONE Agarose Resin
CAT No. 4B-GLU-X *
BINDING CAPACITY > 8 mg recombinant GST /ml gel
ANTIMICROBIAL AGENT 20% Ethanol
STORAGE TEMPERATURE 4 - 8º C
• For all kinds of proteins: small, large protein complex low expression rate proteins.
• Easy changeover: no need for optimization or protocol change.
• Great adaptability: Bulk format suitable for batch, gravity, peristaltic pump & syringe.
• High purity achieved in one purification step, comparable to market standards.
GLUTATHIONE AGAROSE BEADS RESINS
X: Product Quantity (10 or 100 ml). Larger amounts available on request.
Until December 31st 2012GLUTHATIONE PURIFICATION
Get the Best Winter Deals35% DISCOUNT
Experiment: Clarified E.coli lysate containing recombinant Glutathione-S-Transferase, MW 36.100 Da was offered (under the same conditions) to different Glutathione Agarose resins.
Chromatograms and SDS-PAGE analysis indicate similar yields and purity.
Comparative Data
ABT GLUTATHIONE AGAROSE 4B
COMPETITOR
12 (mg purified protein)
11.3 (mg purified protein)
ABT Glutathione Agarose Competitor
[email protected] www.abtbeads.com
S I Z E E X C L U S I O N C H R O M A T O G R A P H Y
A F F I N I T Y C H R O M A T O G R A P H Y A F F I N I T Y C O U P L I N G
E L E C T R O P H O R E S I S R E A G E N T S A C C E S O R I E S
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