Global® for Fertilization

Product Description:

Designed for standard IVF (co-incubation of sperm and oocytes) followed by culture of the embryos in global® from Day 1 onward.

Catalogue Number(s) Unit Size

LGGF-020 20 ml

LGGF-050 50 ml

Intended Use Why choose? Composition Storage Instructions Shelf Life Recommendations

Quality Control Specifications

Intended Use global For Fertilization is designed for holding human cumulus-oocyte complexes prior to conventional fertilization or ICSI, and for conventional in-vitro fertilization.

Why would you choose global® for Fertilization?

For fertilization to occur, a single sperm must pass through the cumulus cells, interact with the zona pellucida, fuse with the oolemma and decondense inside the ooplasm. The fertilization medium must meet the metabolic requirements of the sperm, cumulus cells, and oocyte.

Composition Sodium Chloride

Potassium Chloride

Calcium Chloride

Potassium Phosphate

Magnesium Sulfate

Sodium Bicarbonate

Glucose Lactate Na Salt

Sodium Pyruvate

Amino Acids EDTA Gentamicin

Phenol Red

Storage Instructions Store at 2-8°C and protected from light.

Shelf Life No more than 8 weeks from the date of release when stored unopened at 2-8ºC and

protected from light. For best results, use within four weeks.

Recommendations for Use For a full and detailed recommended protocol of use for global® medium please contact us at customerservice@lifeglobal.com.

Quality Control Specifications Specification Common

Results Physiochemical tests:

• pH (with proper CO2 equilibration)

7.2-7.4 7.3

• Osmolality 260-270 265 Biological Tests

• Lal Endotoxin <0.50 EU/ml <0.03 EU/ml

• Sterility Test, membrane filtration

Negative Negative

1-cell Mouse Embryo Assay (% blastocysts at 96h of culture)

>80% 90-100%

Precautions and Warnings

1. Not to be used for injection 2. Caution: Federal Law (USA)

restricts this device to sale by or on the order of a physician (or properly licensed practitioner).

3. Do not use the product if: • the product packaging appears damaged or if the seal is broken • the expiry date has been exceeded • the product becomes discolored, cloudy, or shows evidence of particulate matter

4. Record on the label: Date Received and Date Opened

5. To avoid problems with contamination, practice aseptic techniques and discard minimal

amounts of excess medium remaining in the bottle.

6. Once the bottle is opened, store the product tightly capped at 2-8ºC and protected from light.