Genomics of Musa: Conservation and Use of Musa ... · AW -Pisang Awak KW – Klutuk Wulung Azhar...
Transcript of Genomics of Musa: Conservation and Use of Musa ... · AW -Pisang Awak KW – Klutuk Wulung Azhar...
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The Banana
Pat Heslop-Harrison www.biobanana.com
Are we going bananas, or where are bananas going?The domestication and future of our most-loved fruit
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What is a banana?Musa – Musaceae – ZingiberalesMonocotyledon – Giant Herb (grass not tree!)
1.1 Banana – Taxonomic position
Zingiberales Order Bed, National Botanic Garden of Wales, 2006
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What is a banana?Monocotyledon – giant herb not a tree!
û ü
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What are bananas?What is in banana DNA?
What is the future for banana?What is the future for diet and
farmers?
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• Guinness Book of Records 2007
• “The banana is the most consumed fruit in the world. It is the 4th most important staple food worldwide and the fifth most important agricultural product after cereals, sugar, coffee and cocoa. The Brits eat 140 million bananas every week!”
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Michael Pillay, IITA
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Uganda
• 400 kg/person/year annual consumption
• Matoke of steamed bananas then mashed
From www.synesthete.com
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Banana Evolution
• Center of origin: South-east Asia
• Grown throughout the humid tropics: Asia, Americas, Africa
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Cultivated banana• Origin from two species:• Musa acuminata (the A genome) and
Musa balbisiana (B genome)
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Challenges in banana breeding
• Sterility &Low fertility and poor seed set & germination
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Banana Plantains Musa
1-7 year plantationVegetatively propagated(exclusively)
85% used as local staple
20-30kg fruit bunch>100Mt /yr
2n=3x=33
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Banana Evolution• Cultivars: sterile,
parthenocarpic clones
• Very unusual for a fruit to be produced without a seed
• Only in last decade for oranges & grapefruit (coming now for lemons and limes)
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• Subsistence agriculture• Smallholder farms• Cash crop• Commercial• Year-round production• Eaten by all ages of people
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Diversity
Plantain AAB
Cooking bananaABB
Michael PilayIITA
Dessertbanana
AAA
Highlandbanana
AAA
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Musa acuminata ‘Calcutta 4’AA genomes, 2n=2x=22One genome and 11 chromosomes from motherOther genome and 11 chromosomes from father
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The banana genome – DNA and Chromosomes
• Haploid genome size:• 500 to 600 Mbp DNA
(Rice: 440 Mbp; Human: 3200 Mbp; Wheat: 17000 Mbp)
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Variety Cavendish• 15% of banana production worldwide• The vast majority of export banana to
temperate countries• Controllable ripening but very sensitive
to conditions• First collected in China in 1826 (Telfair),
Sold to Duke of Devonshire, Chatsworth
• Distributed worldwide from 1836• Became dominant variety in 1960s• Has various variants: Williams, Dwarf C,
Giant C, Grand Naine, Robusta, Poyo …
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L to R: Red - AAA
Palayam codan AAB (two bunch yellow, one green)Peyan ABB (green cooking banana),
Njalipoovan AB (yellow)Robusta AAA (green ripe)
Nendran AABPoovan AAB (one yellow bunch)
Red AAAPeyan
Varkala, Kerala, India
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Measuring diversity
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Where does diversity come from?
• The DNA• Single nucleotide changes
– Cellulose synthase• Deletions/insertions in genes• Duplications
– Modifies expression– Important as gives something for evolution
to work on• Regulatory elements
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ta clone MuG9, genomic, 73268bpaaatccaatcaatccagatcaatattgatcgggttctggacgaagcagtcaaactgatcactaaaattcaatacatgagtgctgatttcagaaacttaatcccttctgatagaacaacttacactaattagtcttaaaactcattaaggttgataaatgtcatattacccttccaggtcataaacagcttatgctgaagctattggcattacacttagtcttaacttctttaacgatatgacaatcaataatgagataggcaaataaaatgacatttttttgaactctgcagaattagctccta
What is a genome?• In bananas and plantains, about 500
million base pairs of DNA
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Cellulose SynthaseSingle Nucleotide Polymorphism SNP
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IRAP diversity in Musa
Teo, Tan, Ho, Faridah, Othman, HH, Kalendar, Schulman 2005 J Plant BiolNair, Teo, Schwarzacher, HH 2006 EuphyticaDesai, Maha…, HH et al. in prep.
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• Yellow AA; Green ABB; Blue BB; Pink AAB; Orange AAA
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The Genepool
• Why do we need it?
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Plant breeding• Keeping up with changes• Biotic stress
– New disease races are continuously appearing and spreading
– Fungi, viruses, bacteria– Insects, nematodes, weeds …
• Abiotic stresses– Drought/flooding/salt, cold …
• Socio-economic changes– More people to feed on less land– Urbanization of population
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• Gros Michel in Fusarium (Panama disease) trial in Malaysia
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4.4 Future – Pollution and land use
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Daily Telegraph 23 May 2006
• No 1 banana could face extinctionBy Roger Highfield, Science Editor
• The most popular type of banana, the Cavendish, is under threat from disease. In the 1950s, Britons ate a different banana, the Gros Michel but it was wiped out by Panama disease.
• Now the Cavendish could follow suit as a new strain of the fungus to which it was supposed to be immune has begun to attack the plants. So far, the new, more aggressive variant of Panama disease - TR4 - has not reached the main exporting countries in Latin America or Africa but it is spreading widely through Cavendish plantations in Asia - Indonesia, Taiwan, southern provinces of China and Malaysia.
• In the humid conditions of traditional banana plantations in Central America, the black Sigatoka fungus which attacks leaves, also thrives and the plants must be protected by weekly sprays of fungicides. Although the Cavendish could disappear, experts are confident that a bunch of alternative bananas could fill the void. The caveat is that the taste and texture will be changed forever and there is likely to be a rise in price.
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• LRRs in Musa compared to reference Rice
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sorghum bicolor C CD229420
Musa S 600162152T1Less200
Arabidopsis
hordeum C BU999438
secale cereale C BE588168-inve
wheat-gi|32774289
Zea
rice-gi|29616647-inversed
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MT1 MT2 AW KW
Primers : MLRR1-F and MLRR2-R
1000 bp
800 bp
600 bp
MT1 and MT2 – Mutiara tolerance to FOCAW - Pisang AwakKW – Klutuk Wulung Azhar Mohamad
& HH 2007
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Banana Streak ParaRetrovirus (BSV)
• Double stranded DNA is infective• Insect vector• Unexpected epidemiology
– Appearance after cold or tissue culture
• Glyn Harper, Roger Hull, IITA,• Ben Lockhart, Andrew Geering• Trude Schwarzacher & HH Leicester
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BSV Expression in Banana
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Glyn HarperJulian OsujiRoger HullHH
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Nuclear Copies of BSV in Banana
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DroughtResponsiveGenes
• Differential display of genes being expressed from droughted and watered Musa lines
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DroughtResponsiveGenes
• Differential display of genes being expressed from droughted and watered Musa lines
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Differential Display
14 DD-PCR reactions using different arbitrary and Oligo dT primer combinations, a total of 22 differentially expressed bands (MDRG)
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Expression Analysis
5% 4% 7%
27%
2%9%
38%
8%
transcription factors
cell division and growth
protein synthesis
novel genes
other
metabolism
abiotic stress related
cellular communicationand signal transduction
Preliminary data; Dhairyasheel Desai, HH et al. in prep 2007
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The Global Musa Genomics Consortium
• To assure the sustainability of banana as a staple food crop by developing an integrated genetic and genomic understanding, allowing targeted breeding, transformation and more efficient use of Musa biodiversity
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Super-domestication:The future of banana crops
• Biotic stresses• Abiotic stresses• Socioeconomic factors
• … all mean current cultivars do not meet future needs
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Super-domestication:The future of banana crops• The genepool has the diversity there
which can meet these challenges
• Breeders need to get better and faster
• Banana, has extra challenges– Staple food– Major income source in many communities– Sterile plant
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How farmers make money
• Stop farming
• Sell something else• Sell the same for more money• Sell more quantity• Reduce costs
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What have farmers done?
• Over the 100 years 1906-2006,
• 1.5% reduction in production costs per year• similar across cereals, fruits, milk, meat,
coal, iron• With increased quality and security,
supporting a longer-lived (3 months/year later that they were born in UK), larger population
• Remarkable total of 10-fold reduction in costs
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What have farmers done?• Increased quality and security, supporting a
longer-lived, larger population
A Century of Change:Trends in UK statisticssince 1900UK House of Commons
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How farmers make money
• (stop farming)
• Sell something else• Sell the same for more money• Sell more quantity for the same amount• Reduce costs
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Are there many candidates?
• 250,000 plants• 4,629 mammals• 9,200 birds• 10,000,000 insects
• But only 200 plants, 15 mammals, 5 birds and 2 insects are domesticated!
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Meat Production
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FAO Statistics 2007
All plant crops with >30M tons annual production
excluding sugar cane and ‘other vegetables’
People: WHO
Calories are pretty important –‘let them eat micronutrients’ is not the message!
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• What are bananas?• What is in banana DNA?
• What is the future for banana?
• What is the future for diet and farmers?
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L to R: Red - AAA
Palayam codan AAB (two bunch yellow, one green)Peyan ABB (green cooking banana),
Njalipoovan AB (yellow)Robusta AAA (green ripe)
Nendran AABPoovan AAB (one yellow bunch)
Red AAAPeyan
Varkala, Kerala, India, 2007
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United Nations Millennium Development Goals
• Goal 1 – Eradicate extreme poverty and hunger • Goal 2 – Achieve universal primary education
• Goal 3 – Promote gender equity and empower women• Goal 4 – Reduce child mortality• Goal 5 – Improve maternal health• Goal 6- Combat HIV/AIDS, malaria and other diseases
• Goal 7 - Ensure environmental sustainability• Goal 8 - Develop a global partnership for
development
Convention on Biodiversity (“Rio Convention”):inventory the worlds diversity