General Microbiology Laboratory Introduction to Micro Lab.
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Transcript of General Microbiology Laboratory Introduction to Micro Lab.
General Microbiology LaboratoryGeneral Microbiology Laboratory
Introduction to Micro Lab
Welcome to Micro Lab
وسلم عليه الله صلى الله رسول قال
ر، ) ال عدوى وال طيرة وال هامة وال صَف�(المجذوم كما تَفر من األسد وفر من
Welcome to Micro Lab
The goal of the laboratory is to expose students to the wide variety of life in the microbial world.
Although the study of microbiology includes bacteria, viruses, algae and protozoa, this lab will concentrate primarily on the bacteria
Microbiological techniques are important in preparing the students for the much harder task of identifying the pathogenic microorganisms in a clinical and environmental specimen.
Each experiment with a brief theoretical introduction
revealing the theoretical basis on which the experiment is based on, so that there will be a strong conjunction between the practical and theoretical sessions.
Also included in this manual, the safety precautions
that each one in the field of microbiology should know and practice
Bacteria belong to the kingdom Monera. This kingdom contains more biological diversity than all other kingdoms combined.
Most people tend to associate bacteria with disease, but less
than ten percent of all bacteria cause disease. Many bacteria cannot even live at the temperatures found in
and on the human body. In this lab, most of the bacteria with which we will be working
are non-pathogenic (do not cause disease). However, some of the bacteria are opportunistic; that is, they can cause disease in an ill or injured person.
Therefore, treat all bacteria as if they are pathogenic (cause
disease).
Laboratory Safety Rules
1. Wear a lab coat in lab. We will be working with a variety of materials that can cause permanent stains on some fabrics. Also, a lab coat can help protect from accidental contamination by microorganisms
No eating or drinking during lab. Many
pathogens spread by ingested food and drink. In addition, food can carry microorganisms that might contaminate laboratory cultures
Laboratory Safety Rules
Keep long or fluffy hair tied up and out of the way. Hair can contaminate and be contaminated by microbial cultures.
Always wear shoes in lab
Thoroughly wash your hands with soap and water before and after lab. Thorough and frequent hand washing easily and effectively controls the spread of many pathogens.
Laboratory Safety Rules
Clean the lab bench with disinfectant before and after lab. This helps to prevent contamination of cultures, books, clothing, etc
Keep the lab bench free of unnecessary materials. Don't use the lab bench as a storage area for coats, books, etc.
Do not take cultures from the lab area
Laboratory Safety Rules
Dispose all contaminated materials in autoclave bags. When in doubt, ask the instructor.
Immediately report all accidents and spills to the instructor. Cover spills with disinfectant-soaked paper towels for at least 15 minutes before disposing them
Read all assigned materials before the lab session. Experiments will go smoother and have greater chances of success when you know what you will be doing ahead of time
Laboratory Safety Rules
Treat all microbial cultures as if they are pathogens. Better safe than sorry
When in doubt, ask the instructor.
The only stupid questions are those that are intended as such.
NOTES
1. Personal belongings are not to be stored in the laboratory.
2. You will be assigned to a group consisting of four students and you will work together in a semester long project
Lab requirements
Lab coatMatches or lighterSoapWaterproof permanent markerSmall or medium size towelSealable plastic bagRubber bandGeneral Microbiology Manual
Acquaint yourself with microbiology lab
Microscopic slides
DettolMicroscope
Staining solutionsBunsen burner
Acquaint yourself with microbiology lab
Sterile cupsWooden tongue depressor and swabs
SyringesNormal saline
Forceps
Acquaint yourself with microbiology lab
AutoclaveIncubator
Acquaint yourself with microbiology lab
Laminar flow Hood
Acquaint yourself with microbiology lab
Culture Plates or Petri Dishes
Plastic Loops and Needles Flaming Nichrome loop
Acquaint yourself with microbiology lab
Anaerobic Jar Candle jar
Acquaint yourself with microbiology lab
Anaerobic station
Acquaint yourself with microbiology lab
Enterotube
API 20 E
Acquaint yourself with microbiology lab
Liquid media in tubes Solid media in tubes
Acquaint yourself with microbiology lab
Different forms of slants
Acquaint yourself with microbiology lab
Blood culture bottles
Acquaint yourself with microbiology lab
Antibiotic disks
Now
Try to Identify all the tools and Equipment around you
END OF LECTURE