DEFINISI, INSTRUMENTASI, PRINSIP KERJA, DAN METODE ANALISIS GAS CROMATOGRAFY MASS SPECTROMETRY (GCMS)DEFINISI, INSTRUMENTASI, PRINSIP KERJA, DAN METODE ANALISISGAS CROMATOGRAFY MASS SPECTROMETRY (GCMS)A. Defenisi Gas Cr!a"#raf$ Mass S%e&"r!e"r$ (GCMS)GCMS !er'%a(an !e")e %e!isa*an sen$a+a r#ani( $an# !en##'na(an )'a !e")e ana,isis sen$a+a $ai"' (r!a"#ra- #as (GC) 'n"'( !en#ana,isis .'!,a* sen$a+a se&ara ('an"i"a"if )an s%e("r!e"ri !assa (MS) 'n"'( !en#ana,isis s"r'("'r !,e(', sen$a+a ana,i".Gas (r!a"#ra- !er'%a(an sa,a* sa"' "e(ni( s%e("rs(%i $an# !en##'na(an %rinsi% %e!isa*an &a!%'ran /er)asar(an %er/e)aan (e&e%a"an !i#rasi (!%nen0(!%nen %en$'s'nn$a. Gas (r!a"#ra- /iasa )i#'na(an 'n"'( !en#i)en"i-(asi s'a"' sen$a+a $an# "er)a%a" %a)a &a!%'ran #as )an .'#a !enen"'(an (nsen"rasi s'a"' sen$a+a )a,a! fase #as.S%e("rs(%i !assa a)a,a* s'a"' !e")e 'n"'( !en)a%a"(an /era" !,e(', )en#an &ara !en&ari %er/an)in#an !assa "er*a)a% !'a"an )ari in $an# !'a"ann$a )i(e"a*'i )en#an !en#'('r .ari0.ari r/i" !e,in#(arn$a )a,a! !e)an !a#ne"i( sera#a!.Pen##'naan (r!a"#ra- #as )a%a" )i%a)'(an )en#an s%e("rs(%i !assa. Pa)'an(e)'an$a )a%a" !en#*asi,(an )a"a $an# ,e/i* a('ra" )a,a! %en#i)en"i-(asian sen$a+a $an# )i,en#a(a%i )en#an s"r'("'r !,e(',n$a.Kr!a"#ra- #as ini .'#a !iri% )en#an )is"i,asi fra(sina,, (arena (e)'a %rses !e!isa*(an (!%nen )ari &a!%'ran "er'"a!a /er)asar(an %a)a %er/e)aan i"i( )i)i* (a"a' "e(anan 'a%). Na!'n, )is"i,asi fra(sina, /iasan$a )i#'na(an 'n"'( !e!isa*(an (!%nen0(!%nen )ari &a!%'ran %a)a s(a,a /esar, se)an#(an GC )a%a" )i#'na(an %a)as(a,a $an# ,e/i* (e&i, ($ai"' !i(r)(Pa1ia23445).6. Ins"r'!en"asi Cr!a"#raf$ Mass S%e&"r!e"r$ (GCMS)Ran#(aian ins"r'!en"asi 'n"'( #as (r!a"#ra- )an s%e(s"rs(%i !assa /er#a/'n# !en.a)i sa"' (esa"'an ran#(aian $an# serin# )ise/'" )en#an GCMS. Se&ara '!'! ran#(aian GCMS 2Ga!/ar 7. Dia#ra! A,ir Kr!a"#ra- Gas0Cair(S'!/er2 *""%288en.+i(i%e)ia.r#8+i(i8Gas9&*r!a"#ra%*$)ia(ses "an##a, 73 Des 3477)6eri('" a)a,a* %en.e,asan !en#enai !asin#0!asin# ins"r'!en" %a)a ran#(aian GCMS.7. Ins"r'!en"asi Gas Kr!a"#ra-a. Carrier Gas S'%%,$Gas %e!/a+a (&arrier #as) %a)a (r!a"#ra- #as san#a",a* %en"in#. Gas $an# )a%a" )i#'na(an %a)a )asarn$a *ar's,a* iner", (erin#, )an /e/as (si#en. Kn)isi se%er"i ini )i/'"'*(an (arena #as %e!/a+a ini )a%a" sa.a /erea(si )an )a%a" !e!%en#ar'*i #as $an# a(an )i%e,a.ari a"a' )ii)en"i-(asi./. In.e(si Sa!%e,Se.'!,a* (e&i, sa!%e, $an# a(an )iana,isis )iin.e(si(an %a)a !esin !en##'na(an se!%ri" (e&i,. Jar'! se!%ri" !ene!/'s ,e!%en#an (are" "e/a, (Le!%en#an (are" ini )ise/'" se%"'!) $an# !ana a(an !en#'/a* /en"'(n$a (e!/a,i se&ara "!a"is(e"i(a se!%ri" )i"ari( (e,'ar )ari ,e!%en#an (are" "erse/'".&. K,!A)a )'a "i%e '"a!a (,! )a,a! (r!a"#ra- #as0&air. Ti%e %er"a!a, "'/e %an.an#)an "i%is /erisi !a"eria, %a)a"an: Ti%e (e)'a, ,e/i* "i%is )an !e!i,i(i fase )ia! $an# /eri(a"an )en#an %a)a /a#ian "er)a,a! %er!'(aann$a. A)a "i#a *a, $an# )a%a" /er,an#s'n# %a)a !,e(', "er"en"' )a,a! &a!%'ran $an# )iin.e(si(an %a)a (,!2M,e(', )a%a" /er(n)ensasi %a)a fase )ia!.M,e(', )a%a" ,ar'" )a,a! &airan %a)a %er!'(aan fase )ia!M,e(', )a%a" "e"a% %a)a fase #as3.Ins"r'!en"asi S%e(s"rs(%i !assaa. S'!/er InSe"e,a* !e,e+a"i ran#(aian #as (r!a"#ra-, sa!%e, #as $an# a(an )i'.i )i,an.'"(an !e,a,'i ran#(aian s%e(s"rs(%i !assa. M,e(',0!,e(', $an# !e,e+a"i s'!/er in ini )iseran# ,e* e,e("rn, )an )i%e&a* !en.a)i inin %si"ifn$a. Ta*a% ini san#a",a* %en"in# (arena 'n"'( !e,e+a"i -,"er, %ar"i(e,0%ar"i(e, sa!%e, *ar's,a* /er!'a"an./. Fi,"erSe,a!a in !e,'i ran#(aian s%e(s"rs(%i !assa, in0in ini !e,a,'i ran#(aian e,e("r!a#ne"i( $an# !en$arin# in /er)asar(an %er/e)aan !asa. Para i,!'+an !e!isa*(an (!%nen0(!%nen !assa 'n"'( (e!')ian )i%i,i* $an# !ana $an# /,e* !e,an.'"(an $an# !ana $an# "i)a( (%rinsi% %en$arin#an). Fi,"er ini "er's !en$arin# in0in $an# /erasa, )ari s'!/er in 'n"'( (e!')ian )i"er's(an (e )e"e("r.&. De"e("rA)a /e/era%a "i%e )e"e("r $an# /iasa )i#'na(an. De"e("r inisasi n$a,a )i.e,as(an %a)a /a#ian /a+a* %en.e,asan ini, !er'%a(an )e"e("r $an# '!'! )an ,e/i* !')a* 'n"'( )i.e,as(an )ari%a)a )e"e("r a,"erna"if ,ainn$a.Da,a! !e(anis!e rea(si, %e!/a(aran sen$a+a r#ani( !er'%a(an *a, $an# san#a" (!%,e(s. Se,a!a %rses, se.'!,a* in0in )an e,e("rn0e,e("rn )i*asi,(an )a,a! n$a,a. Ke*a)iran in )an e,e("rn )a%a" )i)e"e(si.Se,'r'* )e"e("r )i"'"'% )a,a! 1en $an# ,e/i* %anas )i/an)in# )en#an "e!%era"'r (,!. ;a, i"' !en#*en"i(an (n)ensasi )a,a! )e"e("r.;asi, )e"e("r a(an )ire(a! se/a#ai 'r'"an %'n&a(0%'n&a(: se"ia% %'n&a( !e+a(i,isa"' sen$a+a )a,a! &a!%'ran $an# !e,a,'i )e"e("r. Se%an.an# an)a !en#n"r, se&ara *a"i0*a"i (n)isi )a,a! (,!, an)a )a%a" !en##'na(an +a("' re"ensi 'n"'( !e!/an"' !en#i)en"i-(asi sen$a+a $an# "a!%a(0"en"' sa.a an)a a"a' seseran# ,ain "e,a* !en#ana,isa sen$a+a !'rni )ari /er/a#ai sen$a+a %a)a (n)isi $an# sa!a.C. Prinsi% Ker.a Cr!a"#raf$ Mass S%e&"r!e"r$ (GCMS)7. Kr!a"#ra- Gas (Gas C*r!a"#ra%*$)Kr!a"#ra- #as (GC) !er'%a(an .enis (r!a"#ra- $an# )i#'na(an )a,a! (i!ia r#ani( 'n"'( %e!isa*an )an ana,isis. GC )a%a" )i#'na(an 'n"'( !en#'.i (e!'rnian )ari /a*an "er"en"', a"a' !e!isa*(an /er/a#ai (!%nen )ari &a!%'ran. Da,a! /e/era%a si"'asi, GC )a%a" !e!/an"' )a,a! !en#i)en"i-(asi se/'a* sen$a+a (!%,e(s.Da,a! (r!a"#ra- #as, fase $an# /er#era( (a"a'