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GC Column and Mode operation

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temperature controlled oven, door opened to show column capillary column from injector exit to detector

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Types of GC Column Packed Column Capillary Column

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Packed Column stainless steel, nickel, glass, plastics, aluminium. Internal diameter 1.6 to 9.5 mm Length is often 1-3 m Limited Resolution (N< 8,000) Internal column diameter should be at least eight times the diameter of the support particles Densely packed with packing materials or solid support coated with thin layer of stationary liquid phase Diatomaceous earth Size: 60-80 mesh (250-170 m) or 80-100 mesh (170-149 m)

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Packed Column

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Capillary Column Internal diameter 1 mm or less. typically

Jawab Apiezon: dietil keton-> 1-butil bromida -> metil sikloheksana (kenapa 1-butil bromida keluar setelah dietil keton?) OV-17: metil sikloheksana -> dietil keton -> 1-butil bromida Carbowax: metil sikloheksana -> 1-butil bromida -> dietil keton (kenapa dietil keton keluar lebih lama dibandingkan 1-butil bromida?)

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Soal 2 Urutan elusi propanol, 1-propil asetat dan 1-heptana pada kolom apiezon, ov-17 dan carbowax

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Jawab Apiezon: propanol -> 1-propil asetat -> 1-heptana OV-17: propanol -> 1-heptana -> 1-propil asetat (kenapa 1-heptana keluar lebih dulu daripada 1-propil asetat?) Carbowax: 1-heptana -> 1-propil asetat -> propanol

Slide 63

Soal 3 Mengapa resolusi yang baik dengan nilai Rs minimal 1,5? Mengapa dalam GC tekanan sangat penting dan tekanan gas pembawa harus konstan?