GATE Life Sciences 1999

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    GATE- 1.999 I ol It>

    -c======= I:::E SCIENCES JH CHEMISTRY COMPUUORY)

    Gas constant , R = 8.JJ4 J ' K '* OIJ811 I. utm. mur1 K 1

    Farllllll)' wmmt %SIMlcoulomllJ

    Fot each u b - i J u ~ i o n given below, rouraltematives are provided or whiclt only oneroner.t. W1ite the cotTtd answe1in the answerbook by wl'iting a. b. c Ol' d 1on.g with tltecorr esponding sub-question numbe1.

    (20x I = 20)For the two gase. -c ;tnd the r e s p e c t i \ 1 ~mo lecular w e ~ g h t s 1\fe 4H and 23 The

    ~ p o n d i n g ma.,im um hea t c a p a cV

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    ll.2 l\r ' miu-1 To h.we half-life of 10 min.fo r hotJt procc:sses hould hav.: Uleconccnl mliun ur .....0.1 M

    b. O.SMIll 1\1

    d. 1,11 MIn llomic helium the cw o eleclmn presenthu l'e tl s pins in the opposite direction.This ;, 1n nccordnncc With Aulbnu principleb. 0 Cl Oilet11CY of>tom.ic 1b. , . > :2" > ; ; ~

    c 2.'i > 111 > 3"" 3 '" ' 21.3-bttlodiene t\ hen treated with pben?lllluinone in benl'enc at JonLoupcralure (3S"C) lt:olls I l l the tltrmLimcofa, Hydroquiooncb. BellZllldchydec Nplltboloncd. T< tnhydro I - n o p b U u q u i nAmoug fo llowing c - . wllieb willform lnsolubl" ocetyljdc wilb AGa. 2-bucyneb. J pcntyncc. 1-butyncd.1\blch catll it.ar1 in co lwt\n 1 W tJ1 lltu

    m oppropriot c (Inc rn CQ)umn '/(5x = :5)Co lumn It\ Energy of oct t ucionB. 1\lercurous cb lorideC. GruphiteD. Cryo )jleE. GeomtWic isomcrhmColumn 2

    Lny :r 2 1.2-dibrnroo elb""'' \Vnl

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    Haber s processi Arrhenius equation, , calomel7 Rnoull ' s In. Fused sail clecltolysi.sAns\\cr the fo llowmg :A 1'ho nomtnl boiling point of bcnl.l ncnod ctbanol are found to be 79 ,:1"C ond78.2"C J'espect.ivcl) l f thecorrcspoutUug valuus for the cmlwlp)of vupari7lltiou Ill' t e two liquids nre

    311 7f> and 3 1.57 kJ mol-1 calculate theemropy change lor tapori7.alionptocess of lbe II\ o Uquids. Commenton the moleculor stnoctwc or t h ~liquids. (3)B At 25"C. tue ionic product or water(K .) is 5.35 10 1' Whm 11ill be pH at

    this tcmpcrnHue ? (2)1\n;-,, co fo lio ingA n oc standard free energy of lom>ncionof' NO , (g) and N,04(g) nt 27"C 51.31nnd >7.89 kJ mor1 respecti vely .Calculate the K.. for ~ ~ e reactjon2T'C.

    N;0 4g) 2N h g)B. Chlomfonu mod carbon tetruch londofomt an tdcal solution at JOuK trustemperature (he vapor pressure ofchlorofont is 20\1 nlJ l.Hg aod the ofcarbon t c t r n o h l o t i d ~ ls IIG mmllg.Octcnnine the vapor pressure ofsolution C01Haining Cfl.Uimolar m tureof he two components.

    AnS\1 er the fo llo11mg1\. TI1c standard rcductoon fJOLcnunls lorthe reactions are g11cn as follows _

    Sn: +2c-+ Sn t = -tU36 VSn' 1 2c-> Sn E ~ II 1511 VCa iCillate tbe t.G" for the reaction SnJ>4e -+So

    B. 11te mo lar co nductance nt mfi111tcdilution of KCI , KN10 1, and AgNO.pressed ns mho.mol m . ore0.01 450 nnd 0.01.334 respectivelyWhm is the molar conductance ofAgCInt infiJute dilution'?

    A.ns" eothe fo lio" iogA TI1e fo ll01t'i11g nickel complexes.[NiCL.] 1- nud INi(CN)., f are fotu>d tohatc diJTercnt maguetic properties.

    :1 or I''ldeotlf) lhl' onab-neuc properties a:odexplain on the basis o( stnl fieldt h e o ~ [Ni= (Ar( 3d1 4s' l

    B. To an uqueous soluuon of cobullcomplex hs wg tbc formuluCoCh5NH, silter nilrntc solutit>n wasadded. Ho11 m a n ~ moles ofAgCl willbe precipitutcd per mole ofcompkx' Write down the stn1cturc ofthe complex.

    26 Wnte tbe structure ofthecompoUJJds A. B.C D ~ n d E fom1ed to tbc foflowiogsequence of renct ousA. Nicrobeuzcue l.n N ,1 N ~ ~ ~ R\13, lsoprop) I e n ~ e t t e O, . :.._ I E.1 ,O

    I ; BIOCHDIJISTIIYFor each sub-q uestion glwn bclo11 . four:tltemuthes ure pruvidcd of which only one iscnrrect. Write the corn.-cc tl.IISWer in the unswcrbonll by writing n. b, e ur tl nlon: with thecorrcS t)ontHnl suiH1ucstiun number.

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    lh =2 ll)Which or th.: follcm m ~ : sta tenttnsmOOD CCL l" Myoglobm conststs or u smglc

    polypeptide cham 111 additioll lo aheme prosthetic group.b. The red colour associated wUt tho

    liX) gcunted fonns of myoglobitt nudhemoglobi n is due to hemec. Hemoglobin has n higher al'finitr forOX') gcu compared 1.0 myoglobin asseen J'rom oxygen bmding curves.

    d. The

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    t Ccllobi...se) chain is a glytnpoptidc ohain assncintcdw1 th w h i ~ h ,,r the follm\ln limnmnoglobull n>70. Ab. lgGc. lgMd. l >F.Wbich statement ohom ribonuclease 1SC()fi CC I ]u. R ibu nucleuse 1& on exonuclo:asesb. 'l'he onr.ymc m o i c l c n v u occurs

    at pynm1dinc and p u r l ~ uucltohdor c ~ 1 d u e s

    c. Titc hydl\)lysos prnduct 1 oJ"'lynuc lco tide wWt a 5"-phosphatocm inus.

    d. The hydrol,ysis pmduo1 is oy n u ~ m i d c wi th u 3' ph Wiphutctcm inus

    Molecular weight of rut unknown proteincan I 'founJ out byn. E t ~ o t r o p h Q r e s ib. loJJ-bxchoH e chromotngrnploy

    AOinity ChrumntogmphyII- Noneoflhe uho"' t.achutqu esN-lermmnl tuuiuo uoius ' ush:liiJdctenuiJted by Sanger' method usiug- Ni nhydrin Reagentb. 2, 4, dinitrobeuz.encc. Hydt"'.uinecl CQn

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    ;t. Pnkewood ni1r0gcn (PWM)b. Phy10 hcmaggintigin (Pl iA)c. C o n l i n A(con A),1. Sheep fo:d b lood cell (SRBC8)Mo> t u t i b n ore synthesised b) tho:1. Com trol lymphoid organsb. PcriphToll) mphoid org:msc. Ptimnry lymphoid c.rguc"J. J\tac:.roplutg"'Of tlte [uunuuulugkol t.chuiquesco only usedu Enymc-linkc.d i o H n o ~ o r b < : n l oss)(EUSA) " bo used to3ntihndie-; on ly

    h. lmnmnoelectrophoresis can be used todel.:nnine concenh'3lion ol' Gll aus of ts c-athod1c n ~ a t i

    1. . 11h . precipitation. puUctn t\ i.uuuuHo-di:ff USion cannot detect omtigcnic.r l ittionshlp

    d. Radioimmunussa) (RIA) t ust:d forqW 1nt ilntion uf any subsL1n cc that i 'lrmnnno g_cn_\ or hapk..11ic

    m m u n o s u p p r e : s ~ \ ' 1 rnea.sun: e mo.st.....trcctivc. when : dministcrcd lu LJ>rior t.o ont.igcu oxpoureh One \ \ ' < . ~ k before unti_t:n cxvosun:c. Alt bc lime of P un:d. Following antigen exposureWhich s llllomt:n t abcHtt oxidative

    l h i > ~ p h u r n t i o n is not true._ Titc the u e or protein> nndcofctoro lnvolved cndnde the electron

    r c i n complex l-1 \1 and A'l'l 'synLillis .

    b. Tbc mobile cnrricr ub i'luintute liula>complcrtos I ond II with compk< l land C)1oohrome C links complex Illwith complex IV.l'ur """I) two d t m r o l tr.msfem;dCrurn NADH to Q by complWinl -

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    A. In e o u ~ $Olutions below pH 3.0. the~ y n l h c t i c polypept ide po lyglulllmnte.

    o..:gion. ptr

    ~ o o o Clculotc the time req uired torop lic.11e tl1e chromoome. (2)B Suppl) lo you \ \unl to xpre.-ss the

    glohin gene using "" ~ ~ p r e : ; i < m

    l ol il>vector. Wl\ol factor-s are importanl formoximi7ing the eOicienc.y ofr.runscrptl111t nnd translfitit>n if theexpression cctur iL a cukmyotic \'eCtor2. a proklu-yo tic vector3. a thsion prutcirl \ol'tur

    : BOTANYFor r ach u h-qu l'Stion given byllhOI(l;yntht:S is?

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    Caobou dioxideb. Carbon monolcidec. W 1crd C'hlornphyll{'ell w:oll polysaccharides >re synthesi7.edmainly ina. Cytosol1. Golgi p p . r ac. l l n d o p ~ i e reticulumd. Pl:tsnta membraneWht

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    C ~ u r b i t o c e ~ e.t. Rn. .= e

    ~ l i t o ' i . oc.curs bel conu. GJ nd S pheb. S nnd G2 phoseo.. G2lllld G 1piloseu. S pita..: :md Gl plus.:[n tllunL tissue cultu re suulios one of U1cruujor pmblem is tlre production ofpolyphenol in 1he media. 'Ib is cun betocklcd lei Vllrying degre.:s by the mdw.iuni the medi ur:

    Agarb. ViLamons.:,. Sucrosrd. polyvinyl pyrmlidlnen1e enzyme nilrogonase c a 1 a l thetcduclion of tllnitrogen to ammonia. Tillsmolrbdcnon m protcilt roquircKthe foll01>ing of the reaction NADPH. Feb. NADPH, Homec.. Reduced Ferredoxin. A l'P, Fed Nt\01'11. ATP. Herner which ur the fo llow ing e g i i > gnetic sr..:ctrum will ( 'hlo'l)hvll:1bsorb ljght mol\:in1ally 'I

    Greenb. Redc.. Ye llnwcl. Far RedWhich of IJ1e fo llowing phytnhonnonestly in seed gcrm inutiun 'Iu. G i h t , . , . e l l i n . ~b C y t o k . i n i r t\1. ~ " \ i ~d. Ab.crssic ocrdIonoplast id RNA\Vl1icb of lhc following l ~ m c u t . . aboutpla 4 modcs.m.cl:l is correct ?a. Like gap junctions. directly cunneel

    the p b m or3djacent cellsh A new sil > of. mi.,mtnhul" andrnkrofilamerll assembly l h ~ t appearsdw iug l1td tcluplr:r".::. The ~ I J i l a of four to bt Uattcmcd ucsof GuJgi apJ>:tr31lisd. >\ ~ N o n o c c network of fibmu.

    proteint 1hat i$ importnnl In cellructureand lor mirac.,llulur transpelt1

    ln a double sbndard UNA. the possiblenwuber of reading fr:mu:.> isa. 2b. 3c. . jd.

    16. Par themiiJ11 rs K l . : e t ~ d specie.h, on r speciosc. l' lone of he aboved. Bofu 11f the

    17. Whot percentage or the incrdent olar

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    b. 2000l ~ s e presenl in vlantDNA isa, l methyl adenosmeb. 7-methyl cytosmec, j -methyl cytt>sinecL pseudouridine

    2 L Wutclt of lhe toUowing c l t a r a c t e r i s t i ~ isco ' t'Jated with .most monocots bu t not di-cots?a. absence of(eticulate venationb. Hbsenoo s e l s in stem.c presence ,,f lriangulnidc, Ti plnsmtdd. Maize stteak viros

    23 ld.entlly flte compomtd whooe structure isgivettbelow

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    11 ..cH1ouN-- - : - t . 1 ~6 Cll,S N

    a. Abscissic addb. ZeatiJLc. gibberellincL ttiL lJThe l-Jeory of Island Biogeography wasdowoloped bya. Dan,;nb. Wallacec. MacAriJJU[ and WilsoncL None cflhe ' hoveBased on blogeogntp1tlc evtdence, theWallace line-separatesil. Australia .from New Zealandb. Austnilia from Indot;esta

    Austnilia from Japan.

    Sof 16d. Japan fl\om JndvnesiaWhlll are tM uolllllical ,tllD1es fur diefollowin$ economical ly lmp ort:nd Jllrutlsand l" whitn fltg to?a Cotton;b. Rubber,c, rotlee:d. Tobacco;e. CacM (5)

    27. Answer the lollowmg :\Vl1J1l a:rc ruetistems? Mlllltion briotlytibout l:lre .role of meristems .in plantdcvc lopUJcnt (3)

    B. Define the following (2)L Sornetic entbcyogE nesis2. pruthertogenesis

    28. Ali 'wer tlle followutg :\ . How do ceUnlose, pectins aud< J ~ e s di lrl'f' in leun , f j)structure (ii) solnbili ly (3)

    B Wltat ts t te roam di.frerence be.rweenmatl:e tran.spoot Ac md Os'/ (2)

    29. Answer the foUowmg :A Describe briefly the alternation ofgenerations as it a_pptles to floweringplrutts .B. 1n Nettrosporn, a cross between

    n c s ~ n females with normal malesgave an SC tl>Scenl p r o g ~ n y Areciprocal cruss of JIOl' llal a l e ~mth S t1lCSCCI1t males gave all JtoCtllalprogeny. Whatcan yon conclude abou ttlte senes&>nl niutation? (2)

    3() . 1.-i>'t any five devel,lpmenlal processes Iha lareTegulatecl by phytohorrnl1neauXln . (5)K 1111Cil0810LOGY

    :ti'or each sub-question given below. fouralternative answers arc provided of wbkb oneor more are correct. Wr iie the co rrect answerin tltc.uuswer book by "'Tiling a, b, cor d a ou gwith the corr.ft'>ponding sub-questiou nmnbeo.

    (20 XJ :20)L A bnctcri1l1)1 . having doubling lim e i lf 10

    m:im1t..s, fiUs a l i u w ~ . m l ve2>:elcumrletely iu 3 haw'l . Huw muclt lirnew\11 tl lllke to fiJI halfofthe vessel ?a. 80 minutes

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    h 90 minutes" IS miJIUt

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    L c . h r o m n s o m ~ are mod e of DNAh. DNA ;,, oct prt:>Ctll in cyloplnRrnc.. l r : : m ~ r m a t i o n ;and tr.tnsduchon nbaclcno " " c.au.sed by DNA Dnly

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    tl1e culture is in its log phltse ofgrowth . it 1s used to moculnu dhwenmed ium Hich i n ~ ~ . ; t t s 1 1 . ~ ewlc >iHlrce Ill' coohon und tl i n ~ t c : oflactose lll other medium n h

    the samt) . ,\ ssum e thnt alllactose ' ' oonsumed l t tlte item oftrunAf-cr of the o . : . . ~ j U> the ne11medium. O.:plct the n31\tre of th o:growth oJf the haetenal in th""' ' medium. (2)25 . Ans w.,- Ue fo llowing :

    26.

    A. Wril< down u complt:te reac1i\111 (w Ut 1 1 b r n t e p r o d t . s . t111.ymos ~ n d

    of the glycolyti c: pathwayhk h cxh hi1s uh.umi mJOdut mgsulfole to hydl'ogruu ulfidc.(2)

    C. Cortnin sulfote rcduclttg bud1'iil can)out dil]tropvt1 iQ not ion reaction forenetgy melllboli;;m us mg u l l u r~ l l l f l U O < l < f' inlennediatc OX idatiOn

    t(lntc:. \Yritc down the chcmic.tdreactinn u t:d hv the b:ctcria ford iijpmportionatio,; of suUitc i'or i o r ~ (2)

    D..:fuo Ute folluwmg :u .Regulunh. R muiJitlL>c. l' nl;tl>olite repress luoid Boc Ri noonlml

    LaZDOLOOY

    (S , I = S)

    For ea.rh sutHtucsl iou giHn lwlow, f'uur< " ' " u.rc pro,,idcd uf which it

    cnrno.ct. \r\'rire t:hc rorn Ct nn.swcr in tht' answerbnok h) writing " b. c or d ag:oinlion number-

    (20 X I =21)

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    l l uf l l>Titc invett el:orate Petipotus i cons idered tobe a primi ti\e fomt of the o l i o lng Molluscab, ,\JlneJidae. Arthro poda 'nefent(;l'ntA1'he corroct stnlcme:nt nmongsl thelilllnwing is All iol'orrebrates nTe nlo chordal""h. A I vt:rtehmres ore also chonlmesc. All chordates are nlso Yc t1elrntesd. Vertebra eo and c.hordale< arcfYDOil)'lllOUS

    The tenn .turn..,-;c m gcn l giell l limea l c refCili 1

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    d. r P cyc L

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    Co lumn \/1, Jocobfl. Bi hopC. }.fihtoin0 , Oogoll l 1 1ossdsuoCo lwtUI.Bl. Vnnnus2 Staltl3. n ~ k4 . Mun llnwins :A. Why is it believed that mitochondria

    w n . ~ once o fl1le lmng probryotewhich had established a svmbiotic~ b . t i o t l p wi th an :mcic.."tlt uOjccllubr

    cc io each ph>$

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    5.

    1L To ltonsfcr pftosplull.e t the 3' en J oftheONAWhich of the following pro ofGTP?

    ln iationh. Elougutiuu

    TermiJ1JIIond. P t l r a n s l ~ t i o m o d i f i o\\'ltkh .,r the l e>llow ins comp onent>uc o cess,,ry for tllo S)Tllhesi:s of cihc.J W H1cfrom RNA?Ribosome.

    b. DNA pol ym r.tsc. DNA ligose.L TOI>Oisomegc

    6. Which ofUe fo Uowmg will not affect Uteexpression of c loned o rctgn gene bo st1Prom otor strengthh, .;odon choj.:.:b s uofll\a bos.t _genonu.:d. Plilsmid copy numb"r

    7. RNA in" DNA-RI"A hybrid is g ~ l e byu. S1 mtc1c:s eb. RNa is onlyc. V:uious sla gcs ofcell cyc led. homoodun1tin

    I 8. Which of IJ c l'oiiJwin_g

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    19d. IL-4Most lndustriru Enzymes arc oblru.nedfroma. Plan tsb. Animal iiswesc. Microbesd. lnsecis

    20_ In the coovcrsion of Olucasa ro Fmctose1vhich o ( tl\e followutg innn obili:Le

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    ,._ l'IAnti\'IQdiesb. Pl1tnt \1nccincsc. Bioplnsticsd. Plnnt b < ~ b i o r > e Q r ~c. o ...trib" in one sentence why DICI