Gastrulation It is not birth, marriage or death, but gastrulation, which is truly the most important...
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Transcript of Gastrulation It is not birth, marriage or death, but gastrulation, which is truly the most important...
Gastrulation
It is not birth, marriage or death, but gastrulation, which is truly the most important event in your life
Lewis Wolpert
Blastula Stages
1-cell 2-cell 4-cell 8-cell 32-cell
512-cell 1000-cell
Transcription begins
sphere dome 30% epiboly
4 2/3 hrs
Three germ layers
Side view
top view
Skin & nervous system
Muscle & cardiovascular
Gut and liver
Animal pole
Vegetal pole
Slow dorsal migration
Fast dorsal migration
Convergence Extension
Convergence-extension cell movements
Slow dorsal migration
Fast dorsal migration
Convergence Extension
Packing, inhibition of protrusions
Convergence-extension cell movements
Slow dorsal migration
Fast dorsal migration
Packing, inhibition of protrusions
Intercalation
Convergence Extension
Convergence-extension cell movements
Early Development movies
Histone 2B GFP
Made RNA
Injected embryos at the 1-cell stage
Imaged with Digital Scanned Laser Light Sheet Fluorescence Microscopy(capture whole embryo image every 60-90s)
Produced a “digital embryo”
512-cell
Divisions symmetric prior to 512-cell
Divisions asymmetric after 512-cell
Radial waves just after 512-cell
Early Development Movies
Bmp promotes ventral fates
Organizer signals are BMP binding factors: Noggin, Chordin
Bmp signals promote ventral fates (skin, ventral mesoderm) and oppose dorsal fates (neural, dorsal mesoderm)