Fungal cultures
-
Upload
dr-gauri-bapat -
Category
Technology
-
view
533 -
download
7
Transcript of Fungal cultures
Fungal cultures
Dr. Gauri S. Bapat
by
Fungal culture methodology is the foundation of fundamental and applied research as well as the
commercial exploitation of fungi
What do we do to Fungal cultures in laboratory
Growth
Purification
Preservation
Types of substrates for Fungal cultures
Soild substrate
Agar media Liquid mediaAgar-agar- =Othersubstrates
Naturalmedia
Semi-syntheticmedia
Syntheticmedia
Growing Fungi in Laboratory
Limiting Factors for the Growth of Fungi
Temperature Light Aeration Humidity
Why Pure cultures are necessary?
Detect Isolate Identify Quantify Multiply Utilize
Why Preserve Fungal Cultures ?
Fungal cultures are the genetic resource.
Germplasm conservation
Developmental studies
Industrial Application.
Research programs.
Contaminants
Fungi Bacteria Mites
Techniques for elimination
*Serialdilution
*Singlesporeisolation
*Ultra violateirradiation
*Selectivemedium withantibiotic
*Exclusion
*Fumigation
1. Periodic transfer
2. Sterile mineral oil
3. Sterile water
4. Sterile soil or sand
5. Silica gel
6. Drying on organic substrates
7. L-drying
8. Mechanical freezers
9. Lyophilization
Preservation Techniques
Periodic transfer
Advantages Easy Inexpensive Requires no special
equipment
Disadvantages Labor- intensive May induce variation May acquire
contamination Time demanding
Sterile water
Advantages Clean Quick Easy Less expensive Requires no special
equipment Greater genetic
stability Works for all fungi
Disadvantages Viability loss due to
toxin traces in water Viability upto 6 years
Sterile mineral OilAdvantages Easy Less expensive Requires no special
equipment Limits mite-
contamination Viability reported
upto 32 years
Disadvantages Does not work well
for all fungi Retarded growth after
retrieval Messy Risk of contamination
at retrieval
Sterile Soil / Sand/ SilicaAdvantages Less expensive Technically simple Requires no special
equipment Vigor maintained Can produce uniform
inoculum
Disadvantages Work well for
sporulating fungi Viability upto 8 years
Drying on organic substrates
Advantages Less expensive Used for specialized
groups of fungi
Disadvantages Perfection in handling
is essential for ensuring loss of viability
Risk of contamination at retrieval
Lyophilization
Advantages Long viability Ensured protection
against contamination Works well for all
fungi
Disadvantages Expensive technique Complex technique Many copies are
needed as some isolates fail to survive
Viability needs many trials
Broader perspective : Fungal culture collection
system
Culture Accession process
Culture preservation process
Culture maintenance process