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8

http://www.jbc.org/cgi/doi/10.1074/jbc.M110.166850The latest version is at JBC Papers in Press. Published on October 25, 2010 as Manuscript M110.166850

Copyright 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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1.0

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pH 7.35

pH 8

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Inh172

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100

500Inh172

CPTcAMP (µM)

10 30 100

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Inh172

CP

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pH 7.35

genistein (µM)

CPT cAMP

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300 350Inh172

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4

3

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pH 7.35

pH 8

CPTcAMP

10

50 100200

300

350

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genistein (µM)

20 min

50µ

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pH 8

FIGURE 1

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20 µ

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20 min

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1.5

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mutant C491A pH 6 pH 7.35 pH 8

pH 6

pH 7.35

pH 8

WT-

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R C

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C13

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FRT-

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220

120100

FIGURE 2

11


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2 µ

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pH 6

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3002001000

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pH 7.35

pH 8

3.0

2.0

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aliz

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WT

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C1344A

FIGURE 3

12

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C491

V1293

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Raffaella Melani, Valeria Tomati, Luis J.V. Galietta and Olga Zegarra-Morangenistein binding by cytosolic pH

Modulation of cystic fibrosis transmembrane conductance regulator activity and

published online October 25, 2010J. Biol. Chem.

10.1074/jbc.M110.166850Access the most updated version of this article at doi:

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