Phenolic extracts and its antioxidant properties from leaves of Bitter Melon (Mormordica charantia

DivyaSr. ScientistAvian Nutrition and Feed TechnologyCARI, Izatnager Bareilly.

and Health Free radical Structure of Atom

Stable atoms contain an even number of paired electrons.An atom consists of an small, positively charged nucleus surrounded by a cloud of negatively charged electrons.

Electrons in every orbital must be paired and these paired electrons spin in the opposite direction.

It is called stable form of an atomFree radical - what it is? particles withunpaired electron spinning around the nucleus. (can be atom, ions, molecule).Any atom or molecule capable of independent existence (hence the term free) that contains one or more unpaired electronsFree radicals are highly reactive and can cause damage to molecules in the cell

Formation of Free RadicalFree radical are ionized particle in human body

Free radicals produced in NORMAL cellular metabolism are vital to certain body functions, such as fighting disease orinjury. When tissue is diseased or damaged, the bodys immune system sends disease fighting cells to the site, where they produce free radicals in an effort to destroy foreign invaders.

Free radicals are also produced byPollutionUltraviolet light and radiationToxic substancescigarette smoke, overexposure to sunlight3. H 2 O 2 Hydrogen peroxide 1 O 2 Singlet oxygen NOTypes of Free radicalsThere are two major types of free radical species:

reactive oxygen species (ROS)

reactive nitrogen species (RNS). ROS (reactive oxygen species)Free radicals superoxide, O2 -hydroxyl radical, OH peroxyl, ROO alkoxyl, RO hydroperoxyl, HO2 Particals, which are not free radicalshydrogen peroxide, H2O2hypochlorous acid, HClOozone, O3singlet oxygen, 1O2

RNS (reactive nitrogen species)Free radicalsnitrogen oxide, NO .Nitrogen oxide, NO2 .Particals, which are not free radicalsnitrosyl, NO+nitrous acid, HONOnitogen(III) oxide, N2O3peroxynitrite, ONOO -alkylperoxinitrite, ROONOMechanism of radical reactionsRadicals are highly reactive speciesThree distinc steps

initiation (homolytic covalent bonds cleavage) formation of radicalspropagation (chain propagation) in this step required free radical is regenerated repeatedly as a result of chain reactiontermination

Free Radical OxidationStable antioxidantradical breaks the cycle(unstable peroxyl radical)Aldehydes, organic acids, alcohols, ketones(hydroperoxide breakdown products contribute off flavors) R* + O2ROO* ROO* + RHROOH + R* RO* + RHROH + R*

Free radicals damageSteal electrons from cells, DNA, enzymes and cell membranes.

Damage to cell structures, nucleic acids, lipids and proteins.

Lipids in cell membranes are very prone to oxidative damage because some free radicals tend to concentrate in the membrane and cause oxidative damage, known as lipid peroxidationFree radical and diseases Number of human degenerative diseases.Oxidative stress is a harmful condition that occurs when there is an excess of ROS and/or a decrease in antioxidant levels, this may caused tissue damage by physical, chemical, psychological factors that lead to tissue injury in human and causes different diseases Free radical and ageing Aging pigments (lipofusin granules) are accumulated in the subsarcolmal region of the muscle fibers and becomes more abundant with increasing age.

Other diseases such as atherosclerosis, Parkinson's disease and Alzheimer's are also attributed to free radicals.

Need of antioxidantCell produce defense against excessive free radicals by their preventative mechanisms, repair mechanisms, physical defenses and antioxidant defenses.

Antioxidants prevent cell and tissue damage as they act as Scavenger. Food plants including fruits, vegetables and spices are the primary sources of naturally occurring antioxidants for humans

AntioxidantAntioxidants are any substance that delay or inhibitsoxidative damage to a target molecule. At a time oneantioxidant molecule can react with single free radicals and are capable to neutralize free radicals by donating one of their own electrons, ending the carbon-stealing reaction.Antioxidant means "against oxidation." Antioxidants are an inhibitor of the process of oxidation, even at relatively small concentration and thus have diverse physiological role in the body .CLASSIFICATIONEnzymatic antioxidantsNon-Enzymatic antioxidants1. Minerals e.g.-Zinc, Selenium2. Vitamins e.g.-VitaminA, Vitamin C, Vitamin E, Vitamin F3. Carotenoids e.g.--carotene, Lycopene, Lutein, Zeaxanthin4. Low molecular weight Antioxidants e.g.-glutathione,uric acid5. Organosulfur compounds e.g-Allium, Allyl sulfide,Indoles6. PolyphenolsGlutathione reductase,Glucose 6-phosphate dehydrogenase.superoxide dismutaseFlavonoids Stilbenes/ Lignans

Phenolic Acids Photochemical

AnthocyaninsFlavones, Flavanones IsoflavonesFlavonolsFlavanolsCatechinsEpicatechins

Types of natural antioxidantsources of Natural antioxidantPolyphenolic compoundsCarotenoidsVitamin-CVitamin E. Organosulfur compoundsVitamin-A

Mechanism of action of antioxidants

Two principle mechanisms- chain- breaking mechanism by which the primary antioxidant donates an electron to the free radical present in the systems--as beta-carotene and vitamins C and E, or it simply decays into a harmless product.

Preventive - Antioxidant enzymes like superoxide dismutase, catalase and glutathione peroxidase prevent oxidation by reducing the rate of chain initiation. They can also prevent oxidation by stabilizing transition metal radicals such as copper and iron.Three major levels of antioxidant defense in the cellFirst level of defenseSecond level of defenseThird level of defenseLipases, proteases, etc, Free radicalsVitamins A, E, C, carotenoidsGlutathione

Uric acid

Metal-bindingproteinsSe-GSH-Px

CatalaseFree radicals Free radicals Free radicals Levels of antioxidant defense in the cellThe first line of defenseis the preventive antioxidants, which suppress the formation of free radicals. The second line of defenseis the antioxidants that scavenge the active radicals to suppress chain initiation and/or break the chain propagation reactionsThe third line of defenseis the repair andde novoantioxidants. The proteolytic enzymes, proteinases, proteases, and peptidases, present in the cytosol and in the mitochondria of mammalian cells Function Destroying the free radicals that damage cells.Promoting the growth of healthy cells.Protecting cells against premature, abnormal ageing. Help fight age-related macular degeneration. Provide excellent support for the bodys immune system.prevent the oxidation of unsaturated fatsAntioxidant defense system

Antioxidants act as radical scavenger, hydrogen donor, electron donor, peroxide decomposer, singlet oxygen quencher, enzyme inhibitor, metal-chelating agents. Antioxidants in Human health

Considerable research demonstrates the human health benefits of naturally occurring antioxidant compounds. Claims of Various properties anti-viral, anti-inflammatory, anti-cancer, anti-mutagenic, anti-tumour, hepatoprotective

TBARS value of chicken nuggets The rate of lipid oxidation can be effectively retarded by the use of antioxidants.Natural extracts with antioxidant properties.Natural extracts with antioxidant propertiesThe antimicrobial properties of essential oils extracted from plants used against a wide range of microorganisms have been considered as an alternative to antibiotics in livestock. Therefore, essential oils are promising as feed additives to improve feed efficiency and control the spread of pathogens in livestockThe human intake of green tea decreases total cholesterol, increases the high-density lipoprotein (HDL) fraction, and decreases lipoprotein oxidation.

Antioxidants in Poultry healthHatching time is an environmental stress for the chick. At this time stress caused by environment is very height.

Though natural antioxidants level maximum at this stage but in the cycle process enhance lipid unsaturation in tissues.

Low level concentrations of ascorbic acid (can limit vitamin E recycling) and high temperature and humidity increase risk of lipid peroxidation Effect on egg production

In the production system time between the egg laid and cooling is variable. Whatever effort may be made some changes are bound to happen in composition of egg due to high temperature and time gap.

Free radical is responsible accelerating these compositional change and damage to lipids and proteins. Addition of antioxidant in feed may retard such changes ultimately improve egg production.

..Antioxidants in Poultry healthAntioxidants in Poultry healthTransport of chicks: Transportation from hatchery to farm is another source of stress. For breeding companies where chicken transportation could involve several thousand miles, a very high degree of stress would be associated with temperature fluctuation and dehydration.

Oxidation of fat in feed: Oxidized fat in the diet can cause oxidative stress in the intestine increasing antioxidant nutrient requirements. When a chicken diet includes spent fat after its high temperature treatment, the fat usually contains peroxides and hydroperoxides that can contribute substantially to oxidative stress. It is necessary to evaluate benefits vs disadvantages of using such fat sources.

..Antioxidants in Animal healthVaccination stress: Vaccination is also a substantial stress; and in some cases using vitamin E, for example, as a vaccine adjuvant can help improve vaccination efficiency.Exposure to Mycotoxin: Mycotoxins can substantially decrease antioxidant assimilation from the feed and increase their requirement to prevent damaging effects of free radicals produced as a result of mycotoxin exposure.Applications of some antioxidant in poultry production

Increased antioxidant supplementation for improvement of meat quality during storage.

It is recorded that vitamin E and selenium combination is very effective to reduce drip loss.

Decline in egg production due to heat related stress is increased due to less Vitamin E in feed.

Increased antioxidant supplementation decreased mycotoxin toxicity.

continue--Poultry has become a very competitive business and any addition on coast or change in feed ingredient will affect the cost of production and quality of the products. While adding antioxidant in the feed one has to be careful about coast, age, feed and food laws of the country. Certainly there are reports available for beneficial effect of antioxidants improving the immunity system, health management and quality of the poultry products but all of them cannot be introduced in poultry feed. Most precise study has been undertaken on available antioxidants and their scope of application in poultry feed.

continue--On the other hand, under conditions of oxidative stress where free radical production dramatic increases, then without external help it is difficult to prevent damage to major organs and systems. This external help takes the form of increased dietary supplementation of natural antioxidants. For the poultry business it need to understand at the time of feed formulator when and how much fed additive is needed and economically justified.

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