François Jacob (right) and Jaques Monod
description
Transcript of François Jacob (right) and Jaques Monod
François Jacob (right) and Jaques Monod
LacZ:-galactosidaseLacY:-galactoside permease LacA: thiogalactoside transacetylase
LacI: lactose repressor
LacZ’ = alpha fragment located on cloning plasmid
LacZ’’ = omega fragment located in chromosome
IPTGIsopropyl-thio-galactoside
X-Gal5-bromo-4-chloro-3-
indolyl--D-galactoside
X-Gal
Blue precipitate
http://fajerpc.magnet.fsu.edu/Education/2010/Lectures/25_DNA_Replication_files/image008.jpg
http://fajerpc.magnet.fsu.edu/Education/2010/Lectures/25_DNA_Transcription_files/image008.jpg
http://www.nsci.plu.edu/~mivey/4072002/powerpoint/DNA%20Sequencing/img014.GIF
Navigating the human genome web sites
Starting points:
• http://www.ncbi.nlm.nih.gov/entrez/• http://www.ncbi.nlm.nih.gov/projects/genome/guide/human/
• http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=gene
• http://ca.expasy.org/tools/protparam.html
Reverse transcriptase and repeated sequences