For Research Use Only PEX14 Polyclonal ANTIBODY · 2020. 7. 18. · PEX14 Polyclonal ANTIBODY...
Transcript of For Research Use Only PEX14 Polyclonal ANTIBODY · 2020. 7. 18. · PEX14 Polyclonal ANTIBODY...
![Page 1: For Research Use Only PEX14 Polyclonal ANTIBODY · 2020. 7. 18. · PEX14 Polyclonal ANTIBODY Catalog Number:10594-1-AP 19 Publications For Research Use Only Basic Information Catalog](https://reader034.fdocuments.net/reader034/viewer/2022051606/6018222de16cbb698e13f3f9/html5/thumbnails/1.jpg)
PEX14 Polyclonal antibodyCatalog Number:10594-1-AP 26 Publications
For Research Use Only
www.ptglab.com
Basic Information Catalog Number:10594-1-AP
Size:150UL , Concentration: 333 μg/ml byBradford method using BSA as thestandard;
Source:Rabbit
Isotype:IgG
Immunogen Catalog Number:AG0932
GenBank Accession Number:BC006327
GeneID (NCBI):5195
Full Name:peroxisomal biogenesis factor 14
Calculated MW:41 kDa
Observed MW:57 kDa
Purification Method:Antigen affinity purification
Recommended Dilutions:WB 1:2000-1:10000 IP 0.5-4.0 ug for IP and 1:500-1:1000for WBIHC 1:50-1:200 IF 1:50-1:500
Applications Tested Applications:FC, IF, IHC, IP, WB, ELISA
Cited Applications:IF, IHC, WB
Species Specificity:human, mouse, rat
Cited Species:human, monkey, mouse, rat
Note-IHC: suggested antigen retrieval withTE buffer pH 9.0; (*) Alternatively, antigenretrieval may be performed with citratebuffer pH 6.0
Positive Controls:
WB : A431 cells, HEK-293T cells, HeLa cells, SKOV-3cells
IP : mouse liver tissue,
IHC : human liver cancer tissue, human cervical cancertissue
IF : HepG2 cells, COS7 cells, HeLa cells
Background Information PEX14 (peroxisomal biogenesis factor 14) is a peroxisomal membrane protein that is essential for protein dockingonto the peroxisomes. It is a central component of the peroxisimal matrix protein import machinery and interactswith PEX5 and PEX19. PEX14 is ubiquitously expressed and defects in PEX14 are the cause of peroxisome biogenesisdisorder complementation group K (PBD-CGK). This antibody can be used to detect endogenous PEX14 with anapparent molecular weight of 57 kDa (PMID: 16449325; 9653144) and recognize peroxisomal structures in human,monkey and mouse cells.
Notable Publications Author Pubmed ID Journal Application
Fabian Schueren 25247702 Elife IF
José A Nicolás-Ávila 32937105 Cell IF
Thoms Sven S 21741480 J Struct Biol IF
Storage Storage:Store at -20°C. Stable for one year after shipment.Storage Buffer:PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Aliquoting is unnecessary for -20ºC storage
T: 1 (888) 4PTGLAB (1-888-478-4522) (toll freein USA), or 1(312) 455-8498 (outside USA)
E: [email protected] W: ptglab.com
For technical support and original validation data for this product please contact: This product is exclusively available under ProteintechGroup brand and is not available to purchase from anyother manufacturer.
![Page 2: For Research Use Only PEX14 Polyclonal ANTIBODY · 2020. 7. 18. · PEX14 Polyclonal ANTIBODY Catalog Number:10594-1-AP 19 Publications For Research Use Only Basic Information Catalog](https://reader034.fdocuments.net/reader034/viewer/2022051606/6018222de16cbb698e13f3f9/html5/thumbnails/2.jpg)
Selected Validation Data
Various lysates were subjected to SDS PAGEfollowed by western blot with 10594-1-AP (PEX14antibody) at dilution of 1:5000 incubated at roomtemperature for 1.5 hours.
Immunofluorescent analysis of (-20°C Ethanol)fixed HepG2 cells using 10594-1-AP (PEX14antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemical analysis of paraffin-embedded human liver cancer using 10594-1-AP(PEX14 antibody) at dilution of 1:50 (under 10xlens).
Immunohistochemical analysis of paraffin-embedded human liver cancer using 10594-1-AP(PEX14 antibody) at dilution of 1:50 (under 40xlens).
IP Result of anti-PEX14 (IP:10594-1-AP, 3ug;Detection:10594-1-AP 1:500) with mouse livertissue lysate 4000ug.
1X10^6 HepG2 cells were stained with .2ug PEX14antibody (10594-1-AP, red) and control antibody(blue). Fixed with 4% PFA blocked with 3% BSA(30 min). Alexa Fluor 488-conjugated AffiniPureGoat Anti-Rabbit IgG(H+L) with dilution 1:1500.