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PCR : POLYMERASE CHAIN REACTION
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INTRODUCTION
All methods for PCR are based on the concept of DNA strand complementarity .
PCR is one of the most important technique and it is also a key procedure in numerous basic studies involving DNA molecules.
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A NEW ROLE OF GLYPICAN-3 IN HEPATOCELULAR CARCINOMA
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A NEW ROLE OF GLYPICAN-3 IN HEPATOCELULAR CARCINOMA
GPC3 is a member of the glypican family of glycosylphosphatidylinositol-anchored cell-surface heparan sulfate proteoglycans.
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A NEW ROLE OF GLYPICAN-3 IN HEPATOCELULAR CARCINOMA
GPC3 is highly expressed in HCC cells and tissues. It is thought that GPC3 joins a multiprotein complex, which is composed of the ligand, receptor, GPC3, and probably other proteins.
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A NEW ROLE OF GLYPICAN-3 IN HEPATOCELULAR CARCINOMA
This is the first study to report that GPC3, in conjunction with MMPs and growth signaling molecules, might play an important role in the progression of HCC.
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OBSERVATION
The PCR is an important, useful and fast technique that could help find an appropriate diagnosis before symptom expression.
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CERTAIN LABORATORY TECHNIQUE ALLOWS RAPID DETECTION OF EYE PATHOGENS
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The real-time PCR assay delivered results within two hours, whereas culture results were examined after 48 hours.
CERTAIN LABORATORY TECHNIQUE ALLOWS RAPID DETECTION OF EYE PATHOGENS
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Although PCR has a high risk of false positivity, This may demonstrate a better detection sensitivity in the PCR assay.
CERTAIN LABORATORY TECHNIQUE ALLOWS RAPID DETECTION OF EYE PATHOGENS
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Real-time PCR can accurately and simultaneously detect bacterial and fungal pathogens in a speedy fashion.
CERTAIN LABORATORY TECHNIQUE ALLOWS RAPID DETECTION OF EYE PATHOGENS
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OBSERVATION
The PCR technique should be a requirement for eye pathogens diagnosis, because it leads to a diagnosis and therefore more specific treatments.
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MEDICAL UTILITY
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MEDICAL UTILITY
Polymorphism diagnostic
Characterization for transplants
Establish mutation
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DNA clonation Mutation diagnostic Detection of tumor cells
MEDICAL UTILITY
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Prenatal diagnosis disease Genetic screening Detection of infectious
microorganisms
MEDICAL UTILITY
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BIBLIOGRAPHY
MARTINEZ SANCHEZ,Lina María, Biología Molecular, 5 edición, Medellín, UPB, Fac de Medicina 2009, 139p-147p.
http://www.sciencedaily.com/
releases/2010/05/100510161300.htm
http://www.sciencedaily.com/
releases/2010/08/100802091207.htm
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PICTURE
Mejor es callar y que sospechen de tu poca sabiduría que hablar y eliminar cualquier duda sobre ello.Abraham Lincon