First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China...
Transcript of First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China...
![Page 1: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/1.jpg)
OUC-China 2013
First Step Towards
——Reconstructing the magnetosome membrane in E.coli
Artificial Organelle
![Page 2: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/2.jpg)
Last year…we focused on detection…
Oceanfloat and Oceanfeel——OUC-IGEM 2012
![Page 3: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/3.jpg)
OUC-CHINA 2013This year…
We made a Membranous Organelle in prokaryote!
What can it do ?How did we make it ?
……
![Page 4: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/4.jpg)
Background
Why membrane-bound organelles?
Inside the membrane:
Isolated Environment
On the membrane:
Reaction Site
![Page 5: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/5.jpg)
Outline
1. Project
part1: Artificial organelle
part2: RNA Guardian
2. Future work
3. Judging criteria
4. Human practice
5. Acknowledgements
![Page 6: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/6.jpg)
Analysis
What’s the compartment that we want?
Lipid
Enough space
Anchor protein
Easy to use
Compatible
![Page 7: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/7.jpg)
Analysis
Only Eukaryotes?
Can intracellular membrane exist in prokaryotes?
![Page 8: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/8.jpg)
Analysis
The answer may lie in this species:
Magnetospirillum magneticum
![Page 9: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/9.jpg)
Analysis
Natural intracellular membrane
3D organization of magnetosomesAn ECT reconstruction of Magnetospirillum magneticum sp.AMB-1.
Image courtesy of Zhuo Li & Grant Jensen
![Page 10: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/10.jpg)
Analysis
The magnetosome membrane
Lipid
Enough space
Anchor enzymes
Easy to use
Compatible?……
![Page 11: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/11.jpg)
Intracellular Compartment
Bacteria Culturing
Gene Clustering
Compartment Detection
![Page 12: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/12.jpg)
Bacteria Culturing
Magnetospirillum Magneticum
Microearophilic bacteria---How to culture
Contain magnetism---How to detect
![Page 13: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/13.jpg)
Magnetism Detection
![Page 14: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/14.jpg)
Detection
Technical Note
Channel 1: bacterial through.diameter:40um;length:2000um
Channel 2: bacterial sorting under magnetic field.width:2000um;length:3000umChannel 3:bacterial collection and counting.diameter:40um;length:2000um
The schematic diagram of our
Microfluidic chip
![Page 15: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/15.jpg)
Count
Detection
![Page 16: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/16.jpg)
Models of Magnetic Analysis
Projection
using random function to simulate the
movement process inside the microfluidic chip
introducing a magnetism detection coefficient to
quantify this ability
![Page 17: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/17.jpg)
Simulate the movement process inside the microfluidic chip
Models of Magnetic Analysis
![Page 18: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/18.jpg)
Detection
Final position with/without magnetic field
Dynamic acceleration caused by magnetic field
Drag acceeleration caused by microfluidic chip
![Page 19: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/19.jpg)
introducing a magnetism detection coefficient to quantify this ability
the density distribution of the
bacteria in magnetic field is approxi-
mately a piecewise linear function.
Models of Magnetic Analysis
![Page 20: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/20.jpg)
MagnetospirillumMagneticum
AMB-1
Experimental results
![Page 21: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/21.jpg)
The E.coli
Experimental results
![Page 22: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/22.jpg)
Gene Clustering
Fuction of Magnetosome Island
About R5 Region
Artifical Gene cluster
![Page 23: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/23.jpg)
Organization of Magnetosome Islandin M.magneticum AMB-1
Image courtesy of xuzheng et.
Gene Clustering
![Page 24: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/24.jpg)
Repeats Gene Fragments
Regular Gene Sequence
Too Large to Transform
So, transform only necessary genes in E.coli
Gene Clustering
![Page 25: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/25.jpg)
MamK:Lead invagination derived;
Assembly compartment into chain;
MamI, L, B, Q:Stabilize the compartment chain.
Model for magnetosome formation
Gene Clustering
![Page 26: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/26.jpg)
The design of artificial magnetosome gene cluster.
Gene Clustering
![Page 27: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/27.jpg)
Compartment Detection
![Page 28: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/28.jpg)
Compartment Detection
MamC::GFPfusion protein
MamC is the mostly expressed anchor protein protein among all the MMB associated proteins. GFP, a widely used reporter
![Page 29: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/29.jpg)
mamC::GFP
Compartments!
mamC::GFP&Artificial Gene Cluster
Laser confocal microscopy result of
Control & experimental group
Compartment Detection
![Page 30: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/30.jpg)
RNA Guardian
![Page 31: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/31.jpg)
Energy Wasting!
31
Energy-efficient!
Higher copy
plasmid?RBSPromoter
RNA Guardian Introduction
![Page 32: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/32.jpg)
Degradation
Translating
Not translating
RNA Guardian Introduction
![Page 33: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/33.jpg)
Stabilizing a mRNA
using a ribosome?
RNA Guardian Introduction
![Page 34: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/34.jpg)
RNA Guardian Design
Structure of RNA guardian
A . Structure of part K1059003
B . Structure of part K1059004
![Page 35: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/35.jpg)
RNA Guardian Design
Mode pattern of RNA guardian.
![Page 36: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/36.jpg)
3’
5’
5’ 5’
3’
5’
GFP with lva tag
GFP with lva tag
GFP with lva tag
GFP with lva tag
Circuits
promoter
GFP with lva
tagreporter
5’5’-end mRANAguardran
3’3’-end mRANAguardran
RBS
terminater
Experiment 1
Experiment 4
Experiment 3
Experiment 2
RNA Guardian Design
![Page 37: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/37.jpg)
Comparison of the experimental
and control groups by RFU
Comparison by RFU Relative increasing
RNA Guardian Design
![Page 38: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/38.jpg)
Is our design feasible?Prediction with modeling
RNA Guardian Result
![Page 39: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/39.jpg)
RNA Guardian Modeling RBS Calculator
![Page 40: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/40.jpg)
The Control & Experimental Group 3(only plot the RNA)
The Control Group
The Experimental Group 3
RNA Guardian Modeling RBS Calculator
![Page 41: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/41.jpg)
Simulated result for control & experimental group
The Control Group
Experimental Group 4
RNA Guardian Modeling
![Page 42: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/42.jpg)
Error Detection and Estimation
RNA Guardian Modeling
![Page 43: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/43.jpg)
RNA Guardian Modeling Result
The Stability of mRNA is sorted as:
(RBS0 + CDS + RBS1)(+) > (RBS1 + RBS0 + CDs)(+) > (RBS0 + CDs )
(RBS0 + CDS + RBS1)(+):60% relatively increase theoretically
(RBS1 + RBS0 + CDs)(+):20% relatively increase theoretically
![Page 44: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/44.jpg)
Future
Transmission electron microscopy
1.Transmission Electron Microscopy(TEM)
2.RT-PCR & Catalysis mechanism
3.Make intracellular reactor for health and environment
![Page 45: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/45.jpg)
Part submitted
-?- Name Type Description Designer
W BBa_K1059003 Regulatory Its transcript can prevent the mRNA itself from being degraded by RNaseE. Qiu Wang
W BBa_K1059004 Regulatory Its transcript can prevent the mRNA itself from being degraded byexonuclease. Qiu Wang
W BBa_K1059010 Coding RBS J23106+mamI coding squence Wenjun Wang
BBa_K1059013 Coding RBS B0032+mamB coding squence Wenjun Wang
W BBa_K1059066 Composite GFP-LVA under RNA guardian control Yu Wang
BBa_K1059091 Coding mamB coding sequence Wenjun Wang
BBa_K1059001 Composite GFP-LVA under J23101 control Yu Wang
W BBa_K1059002 Composite GFP-LVA under B0035 control Yu Wang
BBa_K1059005 Regulatory DNA segment whose transcript can prevent mRNA degradation by RNaseE. Xue Sun
BBa_K1059006 Regulatory DNA segment whose transcript can prevent mRNA degradation by RNaseE intwo state . Xue Sun
BBa_K1059011 Coding RBS J23106+mamL coding squence Wenjun Wang
BBa_K1059012 Coding RBS B0032+mamQ coding squence Wenjun Wang
BBa_K1059014 Coding RBS B0032+mamK coding squence Wenjun Wang
BBa_K1059015 Coding Promoter J23106 RBS B0032+mamK coding squence Wenjun Wang
BBa_K1059017 Coding mamL coding squence Wenjun Wang
W BBa_K1059027 Composite GFP-LVA under RNA guardian controld by exonuclease. Qiu Wang
BBa_K1059099 Composite GFP-LVA under RNA guardian control Yu Wang
![Page 46: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/46.jpg)
Achievement & judging criteria
We deserve a Gold medal!
1. Submit a series of new standard Biobrick part and device to
MIT.
2. Design RNA guardian device and prove it works well.
3. Improve a Biobrick BBa_K590015 .
4. Utilize microfluidic chip
5. Build the mathematical model reflecting for magnetic detection.
6.Design an approach to analyze the magnetic of bacteria
quantitatively.
7.Help Tsinghua University
8.Run lots of human practice, sharing, thinking,discussing and
practicing.
![Page 47: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/47.jpg)
Camps & Classes
Brainstorming Winter Camp Camp for high school
Mini-jamboree of Science and
Technology Camp
![Page 48: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/48.jpg)
Visits Between iGEM Teams
Visit From SCAU Visit to Tianjin
Model iGEM in Peking
![Page 49: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/49.jpg)
Acknowledgement
Organizations:
Ocean University of China Qingdao Institute of BioEnergy
Tianjian University Peking University
Bioprocess Technology, Chinese Academy of Sciences
Provincial Engineering Laboratory For Biomass Conversion And Process Integration
France-China Bio-Mineralization and Nano-Structure Laboratory(Biomnsl)
Institute of Oceanology, Chinese Academy of Sciences
Qingdao Institute of Bioenergy and Bioprocess Technology,
Chinese Academy of Sciences
![Page 50: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/50.jpg)
Instructors:Xiao-hua ZhangGuanpin Yang
Advisors:Xu JianLongfei WuTian XiaoXianghong WangYunxiang MaoChenguang LiuWen DongZhenmin BaoShugang DongZhihong TangJie Yu
Yang LiuWei LiuLi KangYong PengWenjie WuPeiran ZhangJiaheng LiWeihong LaiTianhe Wang
Acknowledgement
![Page 51: First Step Towards Artificial Organelle2013.igem.org/files/presentation/OUC-China.pdf · OUC-China 2013 First Step Towards ——Reconstructing the magnetosome membrane in E.coli](https://reader035.fdocuments.net/reader035/viewer/2022081407/60558a0fae61e815f92edd32/html5/thumbnails/51.jpg)
Thank you!
New Artificial Organelle in
prokaryotes