Methyltransferases active on mitochondrial 16S rRNA 1 REPORT ...
FINDING 16S BY JADINE DALEY New Domain. 16S rRNA rRNA Found in all Bacteria Functional role is...
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FINDING 16S BY JADINE DALEY New Domain
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Transcript of FINDING 16S BY JADINE DALEY New Domain. 16S rRNA rRNA Found in all Bacteria Functional role is...
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FINDING 16S
BY JADINE DALEY
New Domain
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16S rRNA
rRNA Found in all Bacteria Functional role is same in all ribosomes Structure changes slowly with time Contains variable and conserved regions
Used as standard for classifying and identifying microbes
Technique pioneered by Carl Woese and George E. Fox in 1977
![Page 3: FINDING 16S BY JADINE DALEY New Domain. 16S rRNA rRNA Found in all Bacteria Functional role is same in all ribosomes Structure changes slowly with.](https://reader035.fdocuments.net/reader035/viewer/2022071807/56649eaa5503460f94baf274/html5/thumbnails/3.jpg)
16S in PCR
In our PCR experiment we...
Mixed our purified DNA with Primers and Master Mix to target 16S rRNA gene
Placed in thermocycler to amplify
Loaded amplified DNA into gel
Ran Gel Electrophoresis
Visualized gel
![Page 4: FINDING 16S BY JADINE DALEY New Domain. 16S rRNA rRNA Found in all Bacteria Functional role is same in all ribosomes Structure changes slowly with.](https://reader035.fdocuments.net/reader035/viewer/2022071807/56649eaa5503460f94baf274/html5/thumbnails/4.jpg)
Celia Moore, PhDProfessor of Psychology, College
of Liberal Arts
Areas of Expertise
Developmental Psychobiology
Behavioral Neuroscience
Hormones and Behavior
Sexual Differentiation