Filters Fluorescence and Life Science · PDF fileGeneral Information. For current product...

and Life ScienceInstrumentation

FluorescenceFilters for

Catalog 2009

For current product listings, specifications, and pricing:www.glenspectra.co.uk • [email protected] • +44 020 8204 9517

Omega Optical celebrates 40 years!

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omega optical has been designing and producing optical filters for fluorescence microscopy and instrumentation longer than any other optical filter company. We are grateful for the advancements in science, technology and outer space made with our products. any project, challenging or simple, we are happy to assist. our guiding philosophy has always been to find solutions. optical filters come in many levels of performance, and are produced using various technologies. omega optical has 40 years of experience working with laboratories and instrument designers to achieve the required performance. along with years of experience, we bring a corporate commitment to cooperatively explore, understand and ultimately refine the solution. We have a strong staff of scientists from the biological field, as well as in chemistry and physics that is essential to gain the highest quality of remote analysis. While this catalog mainly represents filters for fluorescence applications, we also manufacture filters for the following:* Medical Diagnostics: Point-of-Care Instrumentation, Hematology, Macular Degeneration, Real-time Patient Monitoring and others.* Industrial: Environmental Monitoring, Machine Vision Systems, Spectroscopy, lasers, Color Correction, Source leveling and others.* Defense, Aerospace and Astronomy: Infra-Red Vision Systems, targeting Systems, lIDaR , Conflict / Weapon Simulators, outer

Space Based Instrumentation, ground-Based telescope Systems, and others. the entire omega optical staff, from the applications Engineering team, to the Sales team, to the Manufacturing teams are anxious to work with you. our aim is to assure that the most appropriate solution will be provided with the highest quality product.

Pricing We value our relationship with you, our customer. We will strive to produce the best product at the most competitive price, and meet your critical need for performance, quality, and service. While pricing guidelines are reflected on our website, please contact us for best pricing.

Warranty – 3 Years all of the products listed are backed by a MINIMUM 3-year satisfaction warranty. Please note that liability does not include obvious misuse of the product and is limited to repair or replacement of the product purchased. omega optical accepts no liability for consequential or incidental damage caused by use of the product.

How to find the right filter inside this catalog Stock and Standard Filters: these are arranged by fluorophore and emission wavelength on pages 12-18.

By filter set number: Please refer to Filter Set Reference table on page 19.

By center wavelength (CWL) and cut-on and cut-off: Select from a list of excitation and emission filters, and dichroic beamsplitters on pages 20-21 and 3rd millennium 22-23.

By fluorophore: Please see Fluorophore Reference table on pages 24-26.

By application: Fluorescent Proteins (pages 27-28), FREt (page 29), Qdot™ (pages 32-33), M-FISH (page 36), Ratio Imaging (page 37), Multi-band (page 38), Multi-color Imaging (pages 34-35), Real-time Imaging (page 37), Flow Cytometry (pages 43-45).

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Introduction general Info . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

technology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Filter terminology . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

Filters For Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 Filter Descriptions & Nomenclature . . . . . . . . . . . . . . . . . . . . . . . . . 7

Choosing the optimal Filter or Filter Set . . . . . . . . . . . . . . . . . . . . . . . . . 8 typical Epi-fluoresence Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

Frequently asked Questions and answers . . . . . . . . . . . . . . . . . . . . . . . 10-11

Products Stock Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-13

QuantaMaX Filters and Sets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 Quanta-Vivid (Q-Vivid) Filters and Sets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

alPHa Vivid Filters and Sets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13 Standard Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14-18

Filter Sets Reference table . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19 Excitation Filters by Center Wavelength . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20

Dichroic Mirrors by Cut-on . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20-21 Emission Filters by Center Wavelength . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 3rd Millennium Filters by Cut-on and/or by Cut-off . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .22-23 Fluorophore and Best Set Reference table . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24-26 Fluorescent Protein Sets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27-28 Fluorescent Protein Multi-band Sets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28 FREt Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29 optimizing Filter Sets for FREt applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30-31 Quantum Dot Filters and Filter Sets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32-33 Pinkel Sets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34-35 Sedat Sets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35 Photoswitchable Protein Set . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35 M-FISH Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36 Ratio Imaging Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37 Multi-band Sets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 Emission Color Chart for Multi-band Sets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

Caged Compound Sets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40 Multi-Photon Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

Beamsplitters & Mirrors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40 Neutral Density Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

IR Blocking Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 IR-DIC Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

Polarizing Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 Fluorescence Reference Slides . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41

Microscope Cubes and Filter Holders . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 Flow Cytometry Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43-45

Appendix light Sources and Detectors . . . . . . . . . . . . . . . . . . . . . . . . .46

Build-a-Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47 Curv-o-matic . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

How to order . . . . . . . . . . . . . . . . . . . . .47

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General Information

For current product listings, specifications, and pricing:www.glenspectra.co.uk �. [email protected] . +44 020 8204 9517

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Filter Types and Technology

QuantaMAX: omega optical’s QuantaMaX filters are manufactured using state-of-the-art Energetic Process coating technology for high durability, and low temperature sensitivity. these high throughput filters provide excellent results in all applications. they are especially effective in applications where photons are scarce and exposure times are critical. QuantaMaX filter designs consider not just peak filter transmission and fluorophore probability curves, but also optimized band placement in relation to light sources, and blocking in relation to detector sensitivity.

ALPHA Technology: omega optical’s proprietary coating process produces extremely steep slopes and precise edge location of cut-on and cut-off wavelengths. In addition, it provides the ability to place transmission and rejection regions exceptionally close together, and high attenuation between the passband and the rejection band.

3rd Millennium Technology: the most critical feature of filter design is the transition from blocking to transmission. Precise location of band edges, coupled with steep slopes, allows for optimal transmission of spectral energy. 3rd Millennium filters are specified by the critical cut-on and cut-off edges, resulting in much more accurate band placement and bandwidth, compared to industry standard filters that are defined by a Center Wavelength (CWl) and a Bandwidth (FWHM). Multi-band Filters: omega optical is a co-holder of the patent on all multi-band filters; including dual-band, triple-band, and quad-band designs.

Others:

Custom Filters – OEM Production Quantities

For optimum instrument system performance we recommend collaborative engineering for custom filter solutions. omega optical is a leading supplier for fluorescence based instruments and applications including: Microplate Readers, DNa Sequencers & analyzers; gene Chip Readers; Flow Cytometers and Cell Sorters; Real time PCR analyzers; Scanners and Imaging Systems; High-throughput and High-Content Systems; Fluorescence Microscopes, and others.

Custom Filters – Small Quantities

Custom filters in single or small quantities are available to ship in 5 – 10 days from our extensive inventory of components. Visit Build-a-Filter at www.omegafilters.com

Aftermarket filters

If you are in need of a filter that did not come with your original equipment, please go to our Build-A-Filter tool on our website. Select your instrument, tell us your filter wave length, or cut-on or cut-off, and we do the rest. No need to specify physical dimensions. See page 47.

Bandpass FiltersEdge FiltersDichroic MirrorsBeamsplitters

Full MirrorsNeutral Density FiltersRejection Band FiltersColor Correction Filters

anti-Reflective Coatingslongpass FiltersShortpass FiltersHot/Cold Mirrors

Wideband FiltersMulti-band FiltersNarrow-band Filters

omega optical employs the use of 23 active coating chambers and has always utilized a wide variety of coating technologies for complex high-phase thickness thin films. We utilize Physical Vapor Deposition (PVD) with resistive element heating, Ion assisted e-Beam, and Dual Magnetron Reactive Ion Beam Sputtering; the world’s most advanced sputtering technology. No single technology is ideal for every application. as a world-class manufacturer, omega optical knows the attributes and limitations of each and utilizes multiple technologies to achieve best results.

In addition, we consider the following to be important considerations when recommending or designing the filter that’s right for your instrument development: angle of incidence, polarization, substrate material, system speed, and temperature effects.

We have our own In-House optical Polishing, grinding & Configuring and can supply filters in physical dimensions of 5mm – 215mm, Round, Square, RectangularStandard Dimension tolerances +0/-0.2mmCustom / Non-Standard Configurations available

Terminology

Angle of Incidence - the principle wavelength of all types of interference filters decreases as aoI increased.Bandwidth or Full Width Half Max (FWHM) – the spectral distance between the cut-on & cut-off wavelengths of a bandpass filter.Blocking – a measure of how well a filter stops energy of a particular wavelength from passing through. Blocking, or rejection, is expressed in optical density. an increase of 1 optical density represents a factor of 10 decrease in transmission.Center Wavelength (CWL) – the wavelength half way between the cut-on & cut-off wavelengths of a bandpass filter.Cut-Off Wavelength – the point on the falling edge of a shortpass filter or the red edge of a bandpass filter where the transmission drops to 50% of its peak value.Cut-On Wavelength – the point on the rising edge of a longpass filter or the blue edge of a bandpass filter where the transmission reaches 50% of its peak value.Polarization - at non-normal aoI, an interference filter's spectral performance in P-polarized light will differ from its performance in S-polarized light. Reflection – for filters that redirect a portion of the spectrum, such as a dichroic, this is the fraction of energy incident upon the filter at any particular wavelength that is reflected off.Substrate Material Considerations - Fused Silica, Sapphire, Silicon, Float glass, absorptive glassSystem Speed - when filtering a converging rather than collimated beam of light, the spectrum results from the integration of the rays at all of the angles within the cone. the peak wavelength shifts about one-half the value that it would shift in collimated light at the cone's most off angle.Temperature Effects - the performance of an interference filter shifts with temperature changes due to the expansion and contraction of the coating materials. Transmission – the fraction of energy incident upon the filter at any particular wavelength that passes through the filter. Expressed as either a percent (85%) or a fraction of 1 (0.85)

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For current product listings, specifications, and pricing:www.glenspectra.co.uk . [email protected] +44 020 8204 9517

filters for Microscopy

For current product listings, specifications, and pricing:www.glenspectra.co.uk . [email protected] . +44 020 8204 9517

Filter Set General Features •Designedforoveralloptimizationofsignal-to-noise,brightness,andmulti-colordiscrimination. •Excitation&emissionbandsoptimizedforcolorperception. •Holders/cubesavailabletofitmostmicroscopes.

Excitation Filter Features •Bandpassplacementmaximizesexcitationenergywhileminimizingemissionbandcrossover. •BlockingfromUVtothelimitsofCCDdetection(1050nm)tominimizebackground;>/=OD5to>/=OD6. •Blockingdesigninsomefilterssignificantlyincreasesfilterlifebyreducingtransmissionwherenotneeded. •Designedforextremeheattolerance. •High-phasethicknesscoatingsforsuperiorspectralcontrol. •Not recommended for use as an emission filter as design differences may negatively effect image

resolution.

Dichroic Beamsplitter Features •Steepcut-onslopesoptimizeexcitationandemissionperformancewhilemaximizingsignal. •Anti-reflectioncoatingstoincreasetransmissionandenhanceimagesharpness. •Fusedsilicasubstratesforreducedauto-fluorescence. •Substrates:opticallypolishedforimagingapplications.

Emission Filter Features •Bandpass/longpassplacementmaximizesemissionsignalwhileminimizingexcitationbandcrossover. •Anti-reflectioncoatingstoincreasetransmission. •Standardthicknesstominimizeregistrationshiftwhenswitchingfiltersets. •Blockingdesignoptimizedtomaximizeemissionsignal. •Notrecommendedforuseasanexcitationfilterwhenusingawidespectrumlightsource(asinaMercury

Burner)due to incomplete blocking across the full range of the source. For sources such as lasers and some lEDs emission filters may perform adequately as excitation.

Zero Pixel Shift

ZPS is recommended for multi-color applications and results in better discrimination. ZPS is also recommended for applications such as FISH (fluorescence in-situ hybridization), CgH (comparative genomic hybridization), SKY (spectral karyotyping), and co-localization studies.

Please specify when ZPS is required. an additional charge is added to the price of some sets.

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filter Descriptions & nomenclatureExcitation & Emission Filters

Note: FWHM or Full Width Half Max is defined as the bandwidth at 50% of the maximum transmission of the filter. Filter Design Bandpass filters vary in the steepness of their edges and the depth of the blocking outside the passband. •QMAX: Surface coated single substrate designs give steep edges, very high transmission and minimal

registration shifts. •3RD: 3RD Millennium Filter designs are manufactured using proprietary alPHa technology and omega’s

patented hermetic assembly. •AF: alPHa Filter designs are manufactured using omega’s proprietary technology resulting in extremely

steepedges,preciseedgeplacement,andtheoreticalattenuation>OD10. • DF: Discriminating Filter designs have 6 or more interfering cavities, resulting in a bandpass filter with

rectangular shape, very steep edges, and very deep blocking up to optical Density (oD) 6 outside the passband.

•WB: Wide-Band Filter designs are 4 & 5 cavity designs with FWHM greater than 30nm and up to several hundred nanometers.

•NB: Narrow-Band Filter designs are 2-cavity designs with FWHM typically between 0.2 and 8nm.

Dichroic Beamsplitters

Note: Cut-on or cut-off wavelength is defined as the wavelength at which the dichroic is at 50% of its maximum transmission value. While defined by the cut-on or cut-off wavelength, the wavelength ranges of the reflection and transmission bands are more critical to dichroic performance. Filter Design Dichroics are used at non-normal angles of incidence (typically 45 degrees), highly reflecting one specified spectral region while optimally transmitting another. •LP: longpass designs transmit wavelengths longer than the cut-on and reflect a range of wavelengths

shorter than the cut-on. •SP: Shortpass designs transmit wavelengths shorter than the cut-off and reflect a range of wavelengths

longer than the cut-off. •DC: Dichroic designs provide wide regions of both transmission and reflection, exhibiting a high degree of

polarization along with a somewhat shallow transition slope. •DR: Dichroic Reflector designs provide a steep transition slope, low polarization, a wide region of transmission,

and a limited region of reflection. •DCXR: Dichroic designs that provide extended reflection regions.

Miscellaneous •DB: Dual-band filter designs have two passbands and two rejection bands. •TB: triple-band filter designs have three passbands and three rejection bands. •AR: anti-reflection coatings are used on many filters to increase transmission and reduce back reflections.

Center Wavelength Filter Design FWHM/Bandwidth, or Cut On/Cut Off

550XX50

Cut-On Wavelength Filter Design 550DRLP

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choosing the Optimal filter set


Fluorophore(s) •ConsultourFluorophoreReferenceTable(pages24-26)ortheCurv-o-matic on our website for excitation

and emission spectra as well as recommended filter sets.

Application •Whatismostimportantinyourapplication—brightsignal,darkbackground,colordiscrimination, or high

signal-to-noise? While no one design can optimize for all dimensions, many designs provide a solution that gives good overall performance.

•Does your application require customized reflection and transmission specifications for your dichroic? Call us for assistance.

•Doesyourapplicationrequire“imagingquality”filters?All3rdMillenniumandstock/standardfiltersinthiscatalog are suitable for all imaging applications.

Light Source •Whatisyourlightsource—halogen,laser,LED,mercury,xenon?Filtersaredesignedtooptimizeperformance

for different light sources.

Detector •Specify your detector—CCD, PMT, CMOS, film, eye. Filter blocking strategies are designed to optimize

performance for different detectors.

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Curv-o-matica web-based interactive database of standard filter set and component spectral curves, along with fluorophore absorption and emission spectra.

•ClickCurv-o-matic at www.omegafilters.com •Chooseafluorophoreandviewspectralcurve •Chooserecommendedfilterset/component;spectralcurveisoverlaid;downloadASCIIdata •Orderonline

Successful fluorescence imaging requires three filters mounted as a single unit in a filter cube or holder, secured in a fluorescence microscope with the proper lightsource and detector. the excitation filter, positioned normal to the incident light, has a bandpass design that transmits the wavelengths. the filtered excitation light reflects off a long-pass dichroic mirror placed at 45°and excites the fluorophore. the mirror has the unique ability to reflect more than 90 percent of the light within the reflection band while passing more than 90 percent of the light in the transmission region. this directs excitation light and fluorescence emission appropriately within the optical setup.

Following excitation, the fluorophore emits radiation at some longer wavelength, which passes through the dichroic mirror and emission filter into a detector. the emission filter blocks all excitation light and transmits the desired fluorescence to produce a quality image with high signal-to-noise ratio (See below).

In a fluorescence filter cube, the incident light passes through the excitation filter. the filtered light reflects off a dichroic mirror, striking the

fluorophore. the longer-wavelength fluorescence emission passes through the dichroic mirror and emission filter to the detector. the emission

filter blocks stray excitation light, providing bright fluorescence against a dark background.

Detector

Emission Filter

Dichroic Mirror

Light Path EmissionLight Path Excitation

Excitation Filter

Light Source

Sample

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typical epi-fluoresence configuration


frequently Asked Questions and Answers


Q: What’s a safe Angle of Incidence (AOI) range for an interference filter?

A: aoI is a critical parameter to consider when purchasing an interference filter. the primary effect of an increase

in the aoI on an interference coating is a shift in spectral performance toward shorter wavelengths. that is, the

principle wavelength of the filter decreases as the aoI increases. a typical interference filter will exhibit only minor

changes in performance with a tilt of up to 10°. However, for certain narrow band filters and transition edges of

dichroics, this slight shift may cause dramatic performance changes. For advice on how tilt affects performance

please call one of our engineers.

Q: Would a dichroic have better reflection/transmission in S or P?

A: Simply put, reflection is better in S polarized light and transmission is better in P. this characteristic is most

pronounced at the transition edge, where the dichroic is going from high reflection to high transmission.

Q: Why are blocking specs so critical?

A: Mostpeopleknowwheretheirsignalofinterestlies,butsometimesdonotconsiderpotentialsourcesof“noise”.

This“noise”couldbeautofluorescencefromthesample,orthesignalfromanotherfluorophore,orevenenergy

from their light source. Blocking is the feature of a filter that attenuates this unwanted energy and permits the energy

from the signal of interest to pass through. Using filters designed to block unwanted signals can improve signal-to-

noise and robustness of the data.

Q: I want sharp edges, are the 3rd Millennium filters suitable?

A: 3RD M filters are manufactured using omega optical's alPHa technology, which produces very steep edges,

capable of handling most application needs.

Standard 3RD M filters utilize an alPHa gamma edge that has a 3% slope factor. this means that the filter’s cut-on

or cut-off edge will go from 50% peak height to oD 5 by the value: 50% peak height wavelength x (0.03).

3RD M filters can also be manufactured with an alPHa Epsilon edge. this filter has a 1% edge factor and thus will

go from 50% peak height to oD 5 by the value: 50% peak height wavelength x (0.01).

Q: Do my excitation and emission filters need to transmit at the peaks of a fluorophore’s absorption/ emission

probability curve?

A: Not necessarily. although it is usually best to encompass as much of the probability peaks of a fluorophore as

possible, sometimes other limiting factors preclude this solution. one example is a sample with multiple labels that

have significant overlap of their emission peaks. In this case, moving the emission filter off the longer wavelength

fluorophore’s emission peak can improve signal discrimination.

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Q: How do I clean filters?

A: If dust and debris are the primary contaminants, filters can usually be sufficiently cleaned by using dry air (such

as a puff from a pipet bulb) or compressed air. If the filters have oily substances that cannot be easily removed,

either acetone or isopropanol can be used using a soft, lint free applicator, such as a Q-tip or soft lens paper.

Q: What does the arrow on the side of a filter indicate?

A: omega optical filters should be oriented with the arrow pointing in the direction of the light path. In other words,

the arrow points away from the light source and towards the detector.

Q: Can I use an excitation filter as an emitter and vice versa?

A: Due to different design strategies used to optimize the performance of a filter as an excitation or an emission

filter, it is not recommended to use one in place of the other.

Q: Can I use a dichroic from my microscope in a flow cytometer for the same dye?

A: generally, no. Flow cytometers are designed to use dichroic beamsplitters which have different specifications than

a fluorescence microscopy dichroic beamsplitter. When inquiring about a particular dichroic not sold as part of a

filter set, you should always specify its desired application.

Q: I’m using filter set to image Cy5®, but I don’t see any image on the screen and I know I have enough dye

loaded. Is the filter working properly?

A: Probably, yes. Cy5® is a fluorophore which emits at the far end of the visible spectrum (peak at 670nm), this can

make viewing it through the eyepiece of the microscope very difficult and typically a B/W CCD camera or PMt is

needed to detect it. Many CCD cameras come with IR blocking filters housed in front of the chip and attenuate light

from 650nm upwards. this effectively blocks signal from Cy5® and similar dyes from reaching the detector. Consult

your camera’s manual to see if the filter can be switched off line or removed.


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PrODucts

stock filters – QuantaMAX Dual band Filter sets are arranged by fluorophores and emission wavelength.

Type Part # Description

Fluorophores Filter Set # Applications Components

FITC, GFP, Emerald, Turbo GFP QMaX-FItC/tRItC Dual bandpass design for multiple labeling. Exciter QMaX/DBEX 485-560TRITC, DsRed, HcRed, J Red NEW! Dichroic QMaX/DBDR 485-560 Emitter QMaX/DBEM 520-610

Filter sets are arranged by fluorophores and emission wavelength.

BFP, Alexa Fluor® 350, DAPI, QMaX-Blue Wide exciter filter may cause cellular damage in live Exciter QMaX/EX 355-405Hoescsht 33342 & 33258 cell applications. Please inquire about narrow band Dichroic QMaX/DI 410lP excitation to reduce phototoxicity. Emitter QMaX/EM 420-480

eGFP, Alexa Fluor® 488, Cy2®, QMaX-green Exciter QMaX/EX 450-490FITC Dichroic QMaX/DI 500lP Emitter QMaX/EM 510-560

DsRed 2, Alexa Fluor® 546, 555 QMaX-Yellow Emission provides a yellow-orange color to Ds Red2, Exciter QMaX/EX 510-560Cy3®, Rhodamine 2, TRITC tRItC and others. Dichroic QMaX/DI 560lP Emitter QMaX/EM 570-600

Hc Red, Alexa Fluor® 568, 594 QMaX-Red Red emission and good discrimination from egFP Exciter QMaX/EX 530-570Mito-Tracker Red in co-expression systems. Dichroic QMaX/DI 580lP Emitter QMaX/EM 600-650

Alexa Fluor® 647, Cy5® QMaX-FarRed Exciter QMaX/EX 620-650 Dichroic QMaX/DI 660lP Emitter QMaX/EM 670-750

QuantaMAX™ filters and sets Application

low photon count fluorescence, confocal, multi-photon microscopy, live cell imaging.

Design Features QuantaMaX™ filters are designed with consideration of peak filter transmission, fluorophore probability curves, optimized band placement in relation to light sources, and blocking in relation to detector sensitivity. Zero Pixel Shift is a standard feature in these high performance filter sets.

Stock Filtersare available in 25 mm round for excitation and emission filters, and 25.7x36 mm rectangular for dichroic beamsplitters.

SHIPS WITHIN 24 HOuRS!

QMAX Far Red Set for Cy5 and similar dyes

Tran

smis

sion

(%

)

Wavelength (nm)

100 –

90 –

80 –

70 –

60 –

50 –

40 –

30 –

20 –

10 –

0 –540 560 580 600 620 640 660 680 700 720 740 760 780

QMaX Far Red Excitation

QMaX Far Red Dichroic

QMaX Far Red Emission



stock filters – QuantaMAX

For current product listings, specifications, and pricing:www.glenspectra.co.uk . [email protected] +44 020 8204 9517 13

NON STANDARD SIZES AvAILABLE

Call for pricing.

stock filters – Quanta-Vivid Filter sets are arranged by fluorophores and emission wavelength.


Fluorophores Filter Set # Components


Fluorophores Filter Set # Components

Zero Pixel Shift (ZPS™)all single band filter sets are available with Zero Pixel Shift. Please contact one of our sales representatives for assistance.

PricingWe provide the most competitive prices. For current product pricing, please visit www.glenspectra.co.ukor contact us at:

[email protected] +44 020 8204 9517

Alpha vivid filters and sets Application

Best for most imaging applications.

Design Features alPHa technology excitation and emission filters provide steep edges, increased blocking, and reduced auto-fluorescence.

DAPI XF06 optimized for Hg lamp. Exciter XF1005 365WB50Hoechst 33342 & 33258 Dichroic XF2001 400DClPAMCA/AMCA-X Emitter XF3002 450aF65

CFP XF114-2 Exciter XF1071 440aF21SpectrumAqua® Dichroic XF2034 455DRlP Emitter XF3075 480aF30

eGFP, Cy2® XF115-2 long pass emission filter may show more Exciter XF1073 475aF40Fluorescein (FITC) auto-fluorescence. Dichroic XF2010 505DRlPAlexa Fluor® 488 Emitter XF3086 510alP

eGFP XF116-2 Narrow band filters can help to reduce sample Exciter XF1072 475aF20Fluorescein (FITC) auto-fluorescence. also useful for discrimination Dichroic XF2037 500DRlPAlexa Fluor® 488, Cy2® from red emitting fluorophores such as mRFP. Emitter XF3080 510aF23

eGFP, Fluorescein (FITC) XF100-2 Exciter XF1073 475aF40Alexa Fluor® 488 Dichroic XF2010 505DRlPCy2®, DiO, Fluo-4 Emitter XF3084 535aF45

eGFP, Fluorescein (FITC) XF100-3 this set consists of wide bandpass filters for collecting maximal Exciter XF1087 470aF50 Alexa Fluor® 488 excitation and emission energy. It is not as discriminating of Dichroic XF2077 500DRlPCy2®, DiO, Fluo-4 yellow and red emitting fluorophores as the XF100-2 or XF116-2. Emitter XF3105 545aF75

YFP XF104-2 Exciter XF1068 500aF25Rhodamine Green™ Dichroic XF2030 525DRlPAlexa Fluor® 532 Emitter XF3074 545aF35

TRITC XF101-2 long pass emission filter. Exciter XF1074 525aF45Cy3®, Alexa Fluor® 555 Dichroic XF2017 560DRlPMitoTracker® Orange Emitter XF3085 565alP

TRITC, Alexa Fluor® 555 XF108-2 Exciter XF1074 525aF45Cy3®, DsRed2 Dichroic XF2017 560DRlPMitoTracker® Orange Emitter XF3083 595aF60

Texas Red®/Texas Red®-X XF102-2 Exciter XF1067 560aF55Cy3.5® Dichroic XF2029 595DRlPMitoTracker® Red Emitter XF3081 645aF75

Cy5®, Alexa Fluor® 647 XF110-2 It is very difficult to see emissions at these wavelengths Exciter XF1069 630aF50APC (allophycocyanin) with the unaided eye. B/W camera is typically used to Dichroic XF2035 650DRlPDiD (DilC18(5)) capture signal. Emitter XF3076 695aF55

NEW!

Quanta-vivid (Q-vivid) filters and setsomega optical continues to improve its fluorescence filter line with the introduction of NEW! Quanta-Vivid filters and sets. Fluorophore specific, with very steep edges for encompassing the excitation and emission maximums of today's most popular fluorophores, and along with superb transmission in the passbands, allows for high throughput and maximizes photon capture. these filters are produced on fused silica substrates for ultimate image quality and registration, along with zero auto-fluorescence.

stock filters – Alpha Vivid Filter sets are arranged by fluorophores and emission wavelength.



CFP XF401 NEW! Exciter XF1402 440QV21 Dichroic XF2034 455DRlP Emittter XF3401 480QV30

TRITC XF402 NEW! Exciter XF1403 525QV45 Dichroic XF2017 560DRlP Emittter XF3402 645QV75

14


PrODuctsStandard Filters

are available in the following sizes.

Excitation and emission filters: 18, 20, 22, and 25 mm round.

Dichroic beamsplitters: 18 x 26, 20 x 28, 21 x 29, and 25.7 x 36 mm rectangular. Dichroics also available as 18, 20, 22, and 25 mm round.

SHIPS WITHIN 5-10 BuSINESS DAYS!

standard filters Filter sets are arranged by fluorophores and emission wavelength.



Deep uv Combinations XF01 Deep UV excitation/near UV emission Exciter XF1000 254WB25 deep purple color Dichroic XF2000 290DClP Emitter XF3000 330WB60

DAPI XF02-2 Wide band excitation filter with longpass emission Exciter XF1001 330WB80 Hoechst 33342 & 33258 filter Dichroic XF2001 400DClPAMCA/AMCA-X Emitter XF3097 400alP

DAPI XF05-2 good with mercury arc lamp Exciter XF1005 365WB50 Hoechst 33342 & 33258 Dichroic XF2001 400DClP AMCA/AMCA-X Emitter XF3097 400alP

BFP XF129-2 See XF131 for bandpass emission filter Exciter XF1075 387aF28 LysoSensor™ Blue (pH 5) Dichroic XF2004 410DRlP Emitter XF3088 435alP

CCF2 (GeneBLAzer™) XF106-2 Combines blue and green emission colors Exciter XF1076 400aF30 Dichroic XF2040 435DRlP Emitter XF3088 435alP

DAPI XF03 Wide band excitation filter with bandpass emission Exciter XF1001 330WB80 Hoechst 33342 & 33258 filter Dichroic XF2001 400DClP AMCA/AMCA-X Emitter XF3002 450aF65

Cascade Blue® XF10-2 Exciter XF1094 380aF15 LysoSensor™ Blue Dichroic XF2001 400DClP Emitter XF3002 450aF65

BFP, LysoSensor™ Blue (pH5) XF131 Exciter XF1075 387aF28 Dichroic XF2004 410DRlP Emitter XF3002 450aF65

SpectrumBlue® XF11 good for Hg lamp Exciter XF1006 400DF15 Cascade Blue® Dichroic XF2059 415DRlP CPM, Pacific Blue™ Emitter XF3002 450aF65

Cascade Yellow™ XF13-2 Exciter XF1008 405DF40 SpectrumAqua® Dichroic XF2040 435DRlP SYTOX® Blue Emitter XF3091 460alP

Pacific Blue™ XF119-2 Exciter XF1076 400aF30 Dichroic XF2040 435DRlP Emitter XF3078 465aF30

wtGFP XF170 longpass emission filter uses primary excitation peak Exciter XF1076 400aF30 Dichroic XF2040 435DRlP Emitter XF3087 480alP

CFP XF130-2 See XF114-2 for bandpass emission filter Exciter XF1071 440aF21 SpectrumAqua® Dichroic XF2034 455DRlP Emitter XF3087 480alP

Fura Red™, Lucifer Yellow XF77-2 Exciter XF1031 450DF55 LysoSensor™ Green (pH5) Dichroic XF2027 485DRlP Emitter XF3092 500alP

AmCyan XF168 Exciter XF1101 440aF40 Dichroic XF2091 465DRlP Emitter XF3119 505aF50

wtGFP XF169 Bandpass emission filter uses primary excitation peak Exciter XF1076 400aF30 Dichroic XF2040 435DRlP Emitter XF3080 510aF23



PricingWe provide the most competitive prices. For current product pricing, please visit www.omegafilters.com or contact us at:

[email protected]

+1.802.254.2690 (outside USa)

1.866.488.1064 (toll free within USa only)


Call for pricing.




Fura Red™ (high calcium) XF18-2 Broad excitation filter Exciter XF1012 455DF70 DiA (4-Di-16-ASP) Dichroic XF2008 515DRlP Emitter XF3093 515alP

Acridine orange (+RNA) XF19-2 Exciter XF1013 470DF35 Auramine O-Feulgen Dichroic XF2008 515DRlP Emitter XF3093 515alP

Fluoro-Gold™ (high pH) XF08 Brighter emission and background than XF09 Exciter XF1005 365WB50LysoSensor™ Yellow/Blue (pH 3) Dichroic XF2001 400DClP Emitter XF3006 og515

GFP (sapphire) XF76 Exciter XF1008 405DF40Cascade Yellow™ Dichroic XF2006 450DClP Emitter XF3003 520DF40

Alexa Fluor® 430 XF14-2 green emission compared to XF15 Exciter XF1009 425DF45Cascade Yellow™ Dichroic XF2007 475DClPLucifer Yellow Emitter XF3105 545aF75

Alexa Fluor® 430 XF15 Yellow emission compared XF14 Exciter XF1009 425DF45LysoSensor™ Green (pH 5) Dichroic XF2007 475DClPQuinacrine Mustard Emitter XF3006 og515

CoralHue Azami Green XF171 Exciter XF1102 475aF30 Dichroic XF2027 485DRlP Emitter XF3003 520DF40

Fluorescein (FITC) XF22 Higher signal-to-noise than XF25 Exciter XF1099 485DF20Alexa Fluor® 488 Dichroic XF2010 505DRlPCy2®, BODIPY® FL Emitter XF3017 530DF30

YFP XF105-2 See XF104-2 for bandpass emission filter Exciter XF1068 500aF25Rhodamine Green™ Dichroic XF2030 525DRlPAlexa Fluor® 532 Emitter XF3082 530alP

Fluorescein (FITC) XF25 Not suitable for multicolor imaging Exciter XF1099 485DF20Cy2®, Alexa Fluor® 488 Dichroic XF2010 505DRlPBODIPY® FL Emitter XF3018 og530

Fluoro-Gold™ (high pH) XF09 Excellent for multiwavelength work in red Exciter XF1005 365WB50Aniline Blue Dichroic XF2001 400DClP Emitter XF3007 535DF35

LysoSensor™ Green (pH 5) XF71 Exciter XF1031 450DF55Auramine O-Feulgen Dichroic XF2026 495DRlP Emitter XF3007 535DF35

Emerald XF172 Exciter XF1072 475aF20 Dichroic XF2077 500DRlP Emitter XF3084 535aF45

Fluorescein (FITC) XF23 Better photopic color rendition Exciter XF1099 485DF20Alexa Fluor® 488 Dichroic XF2010 505DRlPCy2®, BODIPY® FL Emitter XF3007 535DF35

Spectrum Green® XF144 Exciter XF1011 490DF20 Dichroic XF2008 515DRlP Emitter XF3007 535DF35

SpectrumGreen® #2 XF30 Brighter emission and background than XF99 Exciter XF1080 510DF25Alexa Fluor® 532 Dichroic XF2012 540DRlPBODIPY® 530/550 Emitter XF3021 og550

16


PrODucts




Auramine XF20 Narrow band emission filter Exciter XF1013 470DF35 Dichroic XF2008 515DRlP Emitter XF3014 560DF15

Alexa Fluor® 532 XF99 Yellow emission filter Exciter XF1080 510DF25 Dichroic XF2012 540DRlP Emitter XF3014 560DF15

DsRed2 XF111-2 Exciter XF1077 540aF30 Dichroic XF2015 570DRlP Emitter XF3089 575alP

Spectrum Gold® XF145 Exciter XF1019 535DF35 Dichroic XF2017 560DRlP Emitter XF3022 580DF30

TRITC, SpectrumOrange® XF37 optimized for Hg lamp Exciter XF1020 546DF10Cy3®, Alexa Fluor® 555 Dichroic XF2062 555DRlPMitoTracker® Orange Emitter XF3022 580DF30

TRITC, SpectrumOrange® XF32 Yellow emission filter Exciter XF1019 535DF35Cy3®, MitoTracker® Orange Dichroic XF2015 570DRlPAlexa Fluor® 555 Emitter XF3024 590DF35

TRITC, Cy3®, SpectrumOrange® XF34 Red emission filter Exciter XF1019 535DF35MitoTracker® Orange Dichroic XF2015 570DRlPAlexa Fluor® 555 Emitter XF3016 og590

TRITC, Cy3®, SpectrumOrange® XF38 optimized for Hg lamp Exciter XF1020 546DF10Alexa Fluor® 555 Dichroic XF2015 570DRlPMitoTracker® Orange Emitter XF3016 og590

tdTomato XF173 Exciter XF1103 535aF30 Dichroic XF2015 570DRlP Emitter XF3083 595aF60

Ethidium homodimer-1 (EthD-1) XF28 Exciter XF1080 510DF25 Dichroic XF2009 550DClP Emitter XF3019 605DF50

TRITC, SpectrumOrange® XF33 Photomicrography Exciter XF1019 535DF35Cy3®, MitoTracker® Orange Dichroic XF2015 570DRlPAlexa Fluor® 555 Emitter XF3019 605DF50

Cy3.5® XF41 orange emission filter Exciter XF1022 560DF40MitoTracker® Red Dichroic XF2019 590DRlP Emitter XF3027 610DF20

AsRed 2, mStrawberry XF174 Exciter XF1104 555aF30 Dichroic XF2018 580DRlP Emitter XF3120 610aF50

XRITC Cy3.5®, XF40-2 Exciter XF1022 560DF40MitoTracker® Red SNARF®-1 Dichroic XF2019 590DRlP(high pH), Alexa Fluor® 568/594 Emitter XF3094 610alP

Keima Red XF176 NEW! long pass emission filter to give a rich red color Exciter XF1071 440aF21 in single label schemes Dichroic XF2019 590DRlP Emitter XF3094 610alP

Keima Red XF177 NEW! Same excitation and emission filter as XF176, but blue Exciter XF1071 440aF21 shifted dichroic mirror, multicolor applications Dichroic XF2007 475DClP Emitter XF3094 610alP

Standard Filters










Call for pricing.




Keima Red XF178 NEW! Bandpass version of XF177, multi-label applications Exciter XF1071 440aF21 Dichroic XF2007 475DClP Emitter XF3025 615DF45

Rhodamine B, Rhodamine Red™ XF39 Exciter XF1021 550DF30X-Rhodamine (XRITC) Dichroic XF2018 580DRlPNile Red, CryptoLight CF-5 Emitter XF3025 615DF45

Spectrum Orange® XF146 Exciter XF1017 555DF27 Dichroic XF2018 580DRlP Emitter XF3025 615DF45

Texas Red®/Texas Red®-X XF43 Exciter XF1044 575DF25Alexa Fluor® 594 Dichroic XF2020 600DRlP Emitter XF3028 630DF30

Acridine orange (+RNA) XF21 Exciter XF1014 480DF60Di-4 ANEPPS Dichroic XF2009 550DClP Emitter XF3015 635DF55

Propidium Iodide XF35 Exciter XF1019 535DF35Ethidium bromide Dichroic XF2016 560DClPNile Red Emitter XF3015 635DF55

Texas Red®/Texas Red®-X XF42 Exciter XF1022 560DF40SpectrumRed® Dichroic XF2019 590DRlPAlexa Fluor® 594, CryptoLight CF-6 Emitter XF3015 635DF55

mCherry XF175 Exciter XF1067 560aF55 Dichroic XF2020 600DRlP Emitter XF3081 645aF75

Propidium Iodide XF103-2 Exciter XF1074 525aF45Ethidium bromide Dichroic XF2016 560DClPNile Red Emitter XF3081 645aF75

Fura Red™ XF27 Exciter XF1011 490DF20 Dichroic XF2009 550DClP Emitter XF3012 660DF50

7-Aminoactinomycin D (7-AAD) XF36 Exciter XF1019 535DF35SensiLight P-1 Dichroic XF2016 560DClP Emitter XF3029 660DF32

APC (allophycocyanin) XF44 Exciter XF1024 590DF45BODIPY® 630/650-X Dichroic XF2021 630DRlPCryptoLight CF-2 Emitter XF3029 660DF32

APC (allophycocyanin) XF45 Exciter XF1025 610DF20Bo-DIPY® 630/650-X Dichroic XF2021 630DRlPCryptoLight CF-2, SensiLight P-3 Emitter XF3030 670DF40

Cy5® XF46 Excitation filter optimal for 633 HeNe laser line Exciter XF1026 633NB3.0BODIPY® 630/650-X Dichroic XF2022 640DRlPAlexa Fluor® 633/647 Emitter XF3030 670DF40

Cy5® XF47 Non-visual narrow emission Exciter XF1027 640DF20BODIPY® 630/650-X Dichroic XF2035 650DRlPAlexa Fluor® 660 Emitter XF3031 682DF22

Alexa Fluor® 660/680, Cy5.5® XF138-2 Red Diode & HeNe lasers Exciter XF1085 680aSP Dichroic XF2075 690DRlP Emitter XF3104 690alP

18


PrODucts




Cy5® XF70 Non-visual Broad excitation Exciter XF1030 610DF70BODIPY® 630/650-X Dichroic XF2023 660DRlPAlexa Fluor® 660 Emitter XF3034 690DF40

SpectrumFRed® XF147 Exciter XF1027 640DF20 Dichroic XF2035 650DRlP Emitter XF3034 690DF40

Alexa Fluor® 633/647, Cy5® XF140-2 Hg arc lamp Exciter XF1082 607aF75 Dichroic XF2072 650DRlP Emitter XF3076 695aF55

Alexa Fluor® 680, Cy5.5® XF48-2 Non-visual detection Exciter XF1028 670DF20 an IR sensitive detector must be used Dichroic XF2024 690DRlP Emitter XF3095 700alP

Alexa Fluor® 660/680, Cy5.5® XF141-2 Non-visual detection Exciter XF1095 655aF50 an IR sensitive detector must be used Dichroic XF2082 692DRlP Emitter XF3113 710aF40

Alexa Fluor® 700 XF142-2 Non-visual detection Exciter XF1096 685aF30 an IR sensitive detector must be used Dichroic XF2083 708DRlP Emitter XF3114 730aF30

Alexa Fluor® 750, Cy7® XF143-2 Non-visual detection Exciter XF1097 730aF45 an IR sensitive detector must be used Dichroic XF2084 760DRlP Emitter XF3115 770alP

Standard Filters





XF22 Standard Filter Set for FITCRepresentation of Typical Standard Filter Performance

Tran

smis

sion

(%

)

Wavelength (nm)

100 –

90 –

80 –

70 –

60 –

50 –

40 –

30 –

20 –

10 –

0 –

300 350 400 450 500 550 600 650

XF1015 XF2010XF3017

PricingWe provide the most competitive prices. For current product pricing, please visit www.glenspectra.co.uk or contact us at:

[email protected]

+44 020 8204 9517

19

filter sets reference tableFilter Set # Product Category Page #

QMAX-Blue QuantaMaX 12

QMAX-FITC/TRITC QuantaMaX 12

QMAX-FRed QuantaMaX 12

QMAX-Green QuantaMaX 12

QMAX-Red QuantaMaX 12

QMAX-Yellow QuantaMaX 12

XF01 Vivid Standard 14

XF02-2 Vivid Plus 14, 33


XF04-2 Ratio Imaging 37

XF05-2 Quantum Dots 14, 33

XF06 Vivid Standard 13, 36

XF07 Ratio Imaging 37

XF08 Vivid Classic 15


XF100-2 alPHa Vivid 13, 27



XF10-2 Vivid Standard 14


XF103-2 alPHa Vivid 17



XF106-2 geneBlazer 14









XF124 geneBlazer 37



XF131 Vivid Plus 14, 27

XF13-2 Vivid Plus 14


XF135 Multi-band - Dual 28, 38

XF135-1 Pinkel 34







Filter Set # Product Category Page #






XF151-2 FREt 29

XF152-2 FREt 29

XF153 Photoswitchable Protein 35

XF154 Multi-band - triple 28, 38

XF154-1 Pinkel 34

XF155 Sedat 35

XF156 Sedat 35

XF157 Sedat 35

XF158 FREt 29

XF159 FREt 29


XF160 FREt 29

XF162 FREt 29

XF163 FREt 29

XF164 FREt 29

XF165 FREt 29

XF166 FREt 29

XF167 FREt 29

XF168 alPHa Vivid 14, 27















XF201 M-FISH 36

XF202 M-FISH 36

XF203 M-FISH 36

XF204 M-FISH 36

XF206 M-FISH 36

XF207 M-FISH 36

XF208 M-FISH 36



XF210 M-FISH 36



XF231 M-FISH 36

XF232 M-FISH 36







XF300 Quantum Dots 32

XF301-1 Quantum Dots 32




















XF320 Quantum Dots 33








XF401 Quanta-Vivid 13

XF402 Quanta-Vivid 13












XF50-1 Pinkel 34

XF51 Multi-band - Dual 38


XF52-1 Pinkel 34


XF53-1 Pinkel 34

XF56 Multi-band - triple 38

XF57 Multi-band - Quad Set 38

XF57-1 Pinkel 35


XF59-1 Pinkel 34


XF63-1 Pinkel 34



XF67-1 Pinkel 34


XF68-1 Pinkel 34


XF69-1 Pinkel 34




XF73-2 Caged Compound 40

XF74-2 Caged Compound 40

XF75 Caged Compound 40

XF76 Vivid Standard 15, 27


XF79-2 Ratio Imaging 37

XF88-2 FREt 29

XF89-2 FREt 29



XF93-1 Pinkel 34






Call for pricing.

Note

Filter Sets with a dash indicates revision level.


PrODuctsStock Filtersare available in 25 mm round for excitation and emission filters, and 25.7x36 mm rectangular for dichroic beamsplitters.

Standard Filters are available in the following sizes.Excitation and emission filters: 18, 20, 22, and 25 mm round.Dichroic beamsplitters: 18 x 26, 20 x 28, 21 x 29, and 25.7 x 36 mm rectangular. Dichroics also available as 18, 20, 22, and 25 mm round.

excitation filters by cWl(center wavelength)

Part # Descriptor Stock Standard

XF1000 254WB25 l

XF1001 330WB80 l

XF1093 340aF15 l

QMaX/EX355-405 355-405 l

XF1004 355Ht15 l

XF1005 365WB50 l

XF1094 380aF15 l

XF1401 380DF30 l

XF1057 385-485-560tBEX l

XF1059 386-485-560tBEX l

XF1075 387aF28 l

XF1052 390-486-577tBEX l

XF1058 390-486-577tBEX l

XF1054 390-520DBEX l

XF1055 400-477-580tBEX l

XF1098 400-495-575tBEX l

XF1048 400-500DBEX l

XF1065 400-510-630tBEX l

XF1076 400aF30 l

XF1006 400DF15 l

XF1053 405-490-555-650QBEX l

XF1008 405DF40 l

XF1064 415-535-630tBEX l

XF1301 415WB100 l

XF1009 425DF45 l

XF1100 436-495-582tBEX l

XF1078 436-510DBEX l

XF1201 436aF8 l

XF1079 436DF10 l

XF1071 440aF21 l

XF1402 440QV21 l

XF1101 440aF40 l

QMaX/EX450-490 450-490 l

XF1031 450DF55 l

XF1012 455DF70 l

XF1087 470aF50 l

XF1013 470DF35 l

XF1072 475aF20 l

XF1102 475aF30 l

XF1073 475aF40 l

XF1014 480DF60 l

QMaX/DBEX485-560 485-560DBEX l

XF1063 485-555-650tBEX l

XF1202 485aF20 l

XF1042 485DF15 l

XF1015 485DF22 l

Dichroic beamsplittersby cut-On


XF2000 290DClP l

XF2050 385-485-560tBDR l

XF2041 385-502DBDR l

XF2056 390-510-630tBDR l

XF2003 390DRlP l

XF2047 395-540DBDR l

XF2048 400-477-575tBDR l

XF2046 400-485-558-640QBDR l

XF2045 400-485-580tBDR l

XF2061 400-485DBDR l

XF2051 400-495-575tBDR l

XF2055 400-535-635tBDR l

XF2001 400DClP l

QMaX/DI410lP 410lP l

XF2004 410DRlP l

XF2002 415DClP l

XF2059 415DRlP l

XF2040 435DRlP l

XF2065 436-510DBDR l

XF2090 445-510-600tBDR l

XF2006 450DClP l

XF2034 455DRlP l

XF2091 465DRlP l

XF2007 475DClP l

XF2054 485-555-650tBDR l

XF2039 485-555DBDR l

QMaX/DBDR485-560 485-560DBDR l

XF2027 485DRlP l

XF2043 490-550DBDR l

XF2044 490-575DBDR l

XF2042 490-600DBDR l

XF2026 495DRlP l


XF2037 500DRlP l

XF2077 500DRlP l

XF2010 505DRlP l

XF2031 505DRlPXR l

XF2008 515DRlP l

XF2058 515DRlPXR l

XF2030 525DRlP l

XF2013 540DClP l

XF2012 540DRlP l

XF2203 545DRlP l

XF2009 550DClP l

XF2053 555-640DBDR l

excitation filters by cWl(center wavelength)


XF1050 490-550DBEX l

XF1051 490-577DBEX l

XF1049 490-605DBEX l

XF1011 490DF20 l

XF1068 500aF25 l

XF1088 500aF30 l

QMaX/EX510-560 510-560 l

XF1080 510DF25 l

XF1018 515NB2.0 l

XF1203 520aF18 l

XF1074 525aF45 l

XF1403 525QV45 l

QMaX/EX530-570 530-570 l

XF1103 535aF30 l

XF1019 535DF35 l

XF1077 540aF30 l

XF1204 546aF18 l

XF1020 546DF10 l

XF1062 550-640DBEX l

XF1021 550DF30 l

XF1104 555aF30 l

XF1043 555DF10 l

XF1017 555DF27 l

XF1067 560aF55 l

XF1045 560DF15 l

XF1022 560DF40 l

XF1206 572aF15 l

XF1044 575DF25 l

XF1207 580aF20 l

XF1024 590DF45 l

XF1082 607aF75 l

XF1025 610DF20 l

XF1030 610DF70 l

QMaX/EX620-650 620-650 l

XF1069 630aF50 l

XF1026 633NB3.0 l

XF1208 640aF20 l

XF1027 640DF20 l

XF1095 655aF30 l

XF1046 655DF50 l

XF1210 665aF32 l

XF1028 670DF20 l

XF1085 680aSP l

XF1096 685aF30 l

XF1097 730aF45 l

20



Decoding filter descriptionsMost filter descriptions are identified by center wavelength, filter design code and full width half maximum. For example, a 490DF20 filter has a 490-nm center wavelength, and a 20-nm bandwidth, or a 480- to 500-nm passband (all values are approximate). a 490DF20 passband is 490 ±10 nm, not 490 ±20 nm.




Call for pricing.

21

Dichroic beamsplittersby cut-On


emission filters by cWl(center wavelength)


emission filters by cWl(center wavelength)


XF3000 330WB60 l

XF3097 400alP l

XF3004 405DF43 l

QMaX/EM420-480 420-480 l

XF3088 435alP l

XF3061 445-525-650tBEM l

XF3002 450aF65 l

XF3058 457-528-633tBEM l

XF3063 460-520-602tBEM l

XF3059 460-520-603-710QBEM l

XF3054 460-550DBEM l

XF3069 460-560-690tBEM l

XF3091 460alP l

XF3118 465-535-640tBEM l

XF3078 465aF30 l

XF3116 470-530-620tBEM l

XF3060 470-590DBEM l

XF3099 475-550DBEM l

XF3075 480aF30 l

XF3401 480QV30 l

XF3087 480alP l

XF3068 495-575-700tBEM l

XF3005 495DF20 l

XF3092 500alP l

XF3108 502alP l

XF3119 505aF50 l

QMaX/EM510-560 510-560 l

XF3080 510aF23 l

XF3086 510alP l

XF3043 510WB40 l

XF3067 515-600-730tBEM l

XF3093 515alP l

XF3006 og515 l

XF3056 520-580DBEM l

QMaX/DBEM520-610 520-610DBEM l

XF3003 520DF40 l

XF3055 522-637DBEM l

XF3301 525WB20 l

XF3057 528-633DBEM l

XF3082 530alP l

XF3017 530DF30 l

XF3018 og530 l

XF3079 535aF26 l

XF3084 535aF45 l

XF3011 535DF25 l

XF3007 535DF35 l


XF3074 545aF35 l

XF3105 545aF75 l

XF3021 og550 l

XF3014 560DF15 l

XF3085 565alP l

XF3302 565WB20 l

QMaX/EM570-600 570-600 l

XF3089 575alP l

XF3022 580DF30 l

XF3090 585alP l

XF3303 585WB20 l

XF3024 590DF35 l

XF3016 og590 l

XF3066 595-700DBEM l

XF3083 595aF60 l

QMaX/EM600-650 600-650 l

XF3019 605DF50 l

XF3304 605WB20 l

XF3120 610aF50 l

XF3094 610alP l

XF3027 610DF20 l

XF3110 615aF30 l

XF3025 615DF45 l

XF3020 620DF35 l

XF3009 625DF20 l

XF3028 630DF30 l

XF3015 635DF55 l

XF3023 640DF35 l

XF3081 645aF75 l

XF3402 645QV75 l

XF3305 655WB20 l

XF3029 660DF32 l

XF3012 660DF50 l

QMaX/EM670-750 670-750 l

XF3030 670DF40 l

XF3031 682DF22 l

XF3104 690alP l

XF3034 690DF40 l

XF3076 695aF55 l

XF3095 700alP l

XF3113 710aF40 l

XF3100 710aSP l

XF3114 730aF30 l

XF3115 770alP l

XF3307 800WB80 l

XF3308 840WB80 l

XF2062 555DRlP l


XF2016 560DClP l

XF2017 560DRlP l

XF2032 565DRlPXR l

XF2015 570DRlP l


XF2018 580DRlP l

XF2011 585DRlP l

XF2019 590DRlP l

XF2029 595DRlP l

XF2020 600DRlP l

XF2014 610DRlP l

XF2021 630DRlP l

XF2022 640DRlP l

XF2035 650DRlP l

XF2072 650DRlP l


XF2023 660DRlP l

XF2033 675DCSPXR l

XF2024 690DRlP l

XF2075 690DRlP l

XF2082 692DRlP l

XF2083 708DRlP l

XF2084 760DRlP l

3rd Millenium filtersFilter Type Wavelength Range Transmission Attenuation Range Attenuation OD Size

22


PrODucts3rd Millennium Filters assembly Diameter – 25mm

assembly Height – 5.5mm

Clear aperture – 21.3mm

Substrates – Sio2 substrates with

broadband anti-reflection coating

Longpass Cut-on from 400-700nm >/=90%peak UVtocut-on OD12intheory

at every 10nm oD 6 min 25 mm

Cut-on from 700-800nm

at every 20nm

Cut-on from 800-1100nm

at every 50nm

Shortpass Cut-off from 400-700nm >/=90%peak Cut-offto1.3xcut-off* OD12intheory

at every 10nm oD 6 min 25 mm

Cut-off from 700-800nm

at every 20nm

Cut-off from 800-1100nm

at every 50nm

Bandpass Cut-on + Cut-off from >/=80%peak UVto1.3xcut-off* OD12intheory

400-700nm oD 6 min 25 mm

at every 10nm

700-800nm

at every 20nm

800-1100nm

at every 50nm

* For extended long wavelength attenuation see attenuation Extension

all 3rd Millennium filters are offered every 10nm from 400-700nm, every 20nm from 700-800nm and every 50nm from 800nm to 1100nm.

Attenuation Extension:

When using a silicon detector, in some cases a blocker filter is required to extend the attenuation range to 1100nm nominally. For optimal performance we recommend locating the blocker filter in a position remote from the 3rd Millennium filter. Standard blocker filter is 25mm. other sizes are available.

Design Features Hermetic assembly

Fused Silica Substrates

accurate Band Placement

Steeper Edge Slopes

PricingWe provide the most competitive prices. For current product pricing, please visit www.glenspectra.cO.ukor contact us at:



Call for pricing.

23


3rd Millennium filters are specified by the critical cut-on and cut-off edges, resulting in much more accurate band placement and bandwidth. to specify a filter: longpass – specify a cut-on wavelength Example: Part # 3RD650lPShortpass – specify a cut-off wavelength Example: Part # 3RD 520SPBandpass – specify a cut-on & cut-off wavelength Example: Part # 3RD 580lP-600SP

Automated Manufacturing

3rd Millenium filters are produced in custom engineered coating equipment that progresses from raw material to complete assemblies in a shortened manufacturing cycle. the coating chamber is load-locked so that it remains under stable high vacuum conditions between coating cycles.

Control and design of manufacturing processes lead to yield and product uniformity. Short manufacturing cycles results in controlled inventories and shorter lead times.

Design

3rd Millennium filters are manufactured using omega optical’s proprietary alPHa technology, a process which produces exceptionally steep cut-on and cut-off slopes. this results in more precise location of cut-on and cut-off points, the ability to place transmission and rejection regions extremely close together, and higher attenuation between the passband and the rejection band.

Bandpass filters are made using a combination of an alPHa longpass (cut-on) and alPHa shortpass (cut-off). longpass and shortpass filters are each made using a single surface coating.

Higher Throughput

Passbands can be made wider and still achieve the blocking requirements of narrower, less steep designs. Wider alPHa bandpass filters outperform narrower standard designs by providing improved transmission, better blocking, and higher signal-to-noise.

Optical Density Curve Transmission Curve 16 100

14 3RD Millennium Bandpass

Standard

Fabry Perot

Bandpass

80 3RD Millennium Bandpass

12

Tran

smis

sion

(%

T)

Opt

ical

Den

sity

10 60

8

40 6

4 Standard Fabry Perot Bandpass

20 2

0 0 425 475 525 575 625 675

Wavelength (nm)

500 520 540 560 580 600

Wavelength (nm)


24


PrODucts

Fluorophore EX EM Best Set Stock Standard

fluorophores(A-b)

acgFP1 475 505 XF100-2 l

acridine Yellow 470 550 XF23 l

acridine orange (+DNa) 500 526 XF104-2 l

acridine orange (+RNa) 460 650 QMaX-Blue l

alexa Fluor® 350 347 442 QMaX-Blue l

alexa Fluor® 405 401 421 XF11 l

alexa Fluor® 430 434 540 XF14-2 l

alexa Fluor® 488 495 519 XF100-2 l

alexa Fluor® 500 503 525 XF104-2 l

alexa Fluor® 532 531 554 XF104-2 l

alexa Fluor® 546 556 573 XF108-2 l

alexa Fluor® 555 553 568 XF108-2 l

alexa Fluor® 568 579 604 XF102-2 l

alexa Fluor® 594 591 618 XF102-2 l

alexa Fluor® 610 612 628 XF102-2 l

alexa Fluor® 633 632 647 XF140-2 l

alexa Fluor® 647 653 669 XF110-2

alexa Fluor® 660 663 690 XF141-2 l

alexa Fluor® 680 679 702 XF141-2 l

alexa Fluor® 700 702 723 XF142-2 l

alexa Fluor® 750 749 775 XF143-2 l

alexa Fluor® 488/546 FREt 495 573 XF164 FREt

alexa Fluor® 488/555 FREt 495 568 XF164 FREt

alexa Fluor® 488/Cy3® FREt 495 570 XF165 FREt

allophycocyanin (aPC) 650 660 XF110-2 l

aMCa/aMCa-X 345 445 XF06 l

amCyan1 458 489 XF168 l

7-aminoactinomycin D (7-aaD) 546 647 XF103-2 l

7-amino-4-methylcoumarin 351 430 XF06 l

aniline Blue 370 509 XF09 l

aNS 372 455 XF05-2 l

asRed2 578 592 QMaX-Yellow l

atto-tag™ CBQCa 465 560 XF18-2 l

atto-tag™ FQ 486 591 XF115-2 l

auramine o-Feulgen 460 550 XF20 l

azami green 493 505 QMaX-green l

BCECF 503 528 XF16 l

BFP (Blue Fluorescent Protein) 382 448 QMaX-Blue l

BFP/DsRed2 FREt 382 583 XF159 FREt

BFP/egFP FREt 382 508 XF89-2 FREt

BFP/YFP FREt 382 527 XF158 FREt

BoBo™-1, Bo-PRo™-1 462 481 XF114-2 l

BoBo™-3, Bo-PRo™-3 570 604 XF102-2 l

BoDIPY® Fl 505 513 XF100-2 l

BoDIPY® tMR 542 574 XF108-2 l

BoDIPY® tR-X 589 617 XF102-2 l

BoDIPY® 492/515 490 515 XF100-2 l

BoDIPY® 493/ 503 500 506 QMaX-green l

BoDIPY® 500/ 510 509 515 XF104-2 l


fluorophores(b-c)

BoDIPY® 505/515 502 510 QMaX-green l

BoDIPY® 530/550 533 550 XF108-2 l

BoDIPY® 558/568 558 568 XF108-2 l

BoDIPY® 564/570 564 570 XF108-2 l

BoDIPY® 581/591 582 590 XF102-2 l

BoDIPY® 630/650-X 630 650 XF45 l

BoDIPY® 650/665-X 650 665 XF110-2 l

BoDIPY® 665/676 665 676 XF110-2 l

BtC 401/464 529 XF133 Ratio Imaging

Calcein 494 517 XF100-2 l

Calcein Blue 375 420 XF06 l

Calcium Crimson™ 590 615 XF102-2 l

Calcium green-1™ 506 531 XF104-2 l

Calcium orange™ 549 576 XF108-2 l

Calcofluor® White 350 440 XF06 l

5-Carboxyfluorescein (5-FaM) 492 518 XF100-2 l

5-Carboxynaphthofluorescein 598 668 XF102-2 l

6-Carboxyrhodamine 6g 525 555 XF104-2 l

5-Carboxytetramethylrhodamine (5-taMRa) 542 568 XF108-2 l

Carboxy-X-rhodamine (5-RoX) 574 602 XF102-2 l

Cascade Blue® 400 420 XF06 l

Cascade Yellow™ 402 545 XF106 l

CCF2 (geneBlazer™) 402 520 XF106 l

Cell tracker Blue 353 466 XF06 l

Cerulean 433 475 XF114-2 l

CFP (Cyan Fluorescent Protein) 434 477 XF114-2 l

CFP/DsRed2 FREt 434 583 XF152 FREt

CFP/YFP FREt 434 527 XF88 FREt

Chromomycin a3 450 470 XF114-2 l

Cl-NERF (low pH) 504 540 XF104-2 l

CoralHue azami green 492 505 XF171 l

CoralHue Dronpa green 503 518 Call l

CoralHue Kaede green 508 518 XF153 l

CoralHue Kaede Red 572 580 XF153 l

CoralHue Keima Red 440 620 Call l

CoralHue Kusabira orange (mKo) 552 559 XF108-2 l

CoralHue Midoriishi-Cyan (MiCy) 472 492 XF116-2 l

CPM 385 471 XF11 l

6-CR 6g 518 543 XF104-2 l

Cryptolight CF-2 584/642 657 XF44 l

Cryptolight CF-5 566 597 XF39 l

Cryptolight CF-6 566 615 XF42 l

CtC Formazan 450 630 XF21 l

Cy2® 489 506 XF100-2 l

Cy3® 550 570 XF108-2 l

Cy3.5® 581 596 XF102-2 l

Cy5® 649 670 QMaX-Far Red l

Cy5.5® 675 694 XF141-2 l

Cy7® 743 767 XF143-2 l

XF





Decoding filter descriptionsMost filter descriptions are identified by center wavelength, filter design code and full width half maximum. For example, a 490DF20 filter has a 490-nm center wavelength, and a 20-nm bandwidth, or a 480- to 500-nm passband (all values are approximate). a 490DF20 passband is 490 ±10 nm, not 490 ±20 nm.

PricingWe provide the most competitive prices. For current product pricing, please visit www.glenspectra.cO.uk or contact us at:

[email protected]

+44 020 8204 9517


Call for pricing.

25


fluorophores(c-G)

Cy3®/Cy5.5® FREt 550 694 XF167 FREt

Cy Pet 435 477 XF114-2 l

Cycle 3 gFP 395/478 507 XF76 l

Dansyl cadaverine 335 518 XF02-2 l

Dansylchloride 380 475 XF10-2 l

DaPI 358 461 QMaX-Blue l

Dapoxyl® 373 574 XF05-2 l

Dia (4-Di-16-aSP) 491 613 XF21 l

DiD (DilC18(5)) 644 665 QMaX-Far Red l

DIDS 341 414 XF03 l

Dil (DiIC18(3)) 549 565 XF108-2 l

Dio (DioC18(3)) 484 501 XF100-2 l

DiR (DiIC18(7)) 750 779 XF143-2 l

Di-4 aNEPPS 488 605 XF21 l

Di-8 aNEPPS 468 635 XF21 l

DM-NERF (4.5–6.5 pH) 510 536 XF104-2 l

DsRed2 (Red Fluorescent Protein) 558 583 QMaX-FItC/tRItC l

DsRed-Express 557 579 XF108-2 l

DsRed Monomer 556 586 XF108-2 l

ElF® -97 alcohol 345 530 XF09 l

Emerald 487 509 XF172 l

EmgFP 487 509 XF100-2 l

Eosin 524 544 XF104-2 l

Erythrosin 529 554 XF104-2 l

Ethidium bromide 518 605 XF103-2 l

Ethidium homodimer-1 (EthD-1) 528 617 XF103-2 l

Europium (III) Chloride 337 613 XF02-2 l

5-FaM (5-Carboxyfluorescein) 492 518 XF100-2 l

Fast Blue 365 420 XF06 l

Fluorescein (FItC) 494 518 XF100-2 l

FItC/Cy3® FREt 494 570 XF162 FREt

FItC/Rhod 2 FREt 494 571 XF162 FREt

FItC/tRItC FREt 494 580 XF163 FREt

Fluo-3 506 526 XF104-2 l

Fluo-4 494 516 XF100-2 l

FluorX® 494 519 XF100-2 l

Fluoro-gold™ (high pH) 368 565 XF09 l

Fluoro-gold™ (low pH) 323 408 XF05-2 l

Fluoro-Jade 475 525 XF100-2 l

FM® 1-43 479 598 XF115-2 l

Fura-2 335 505 XF04-2 l

Fura-2/BCECF 335/503 505/528 XF79-2 Ratio Imaging

Fura Red™ 436 637 XF17 Ratio Imaging

Fura Red™/Fluo-3 472/506 672/527 XF26 Ratio Imaging

geneBlazer™ (CCF2) 402 520 XF124 Ratio Imaging

gFP wt 395/ 475 509 XF169 l

egFP 488 508 XF116-2 l

gFP (sapphire) 395 508 XF76 l

egFP/DsRed FREt 470 585 XF151-2 FREt


fluorophores(G-M)

egFP/Rhod-2 FREt 488 571 XF151-2 FREt

HcRed 591 613 XF102-2 l

Hilyte Fluor™ 488 497 525 XF100-2 l





Hoechst 33342 & 33258 352 461 QMaX-Blue l

7-Hydroxy-4-methylcoumarin (pH 9) 360 449 XF06 l

1,5 IaEDaNS 336 482 XF02-2 l

Indo-1 330 401 XF07 l

JC-1 498/593 525/595 XF115-2 l

6-JoE 525 555 XF104-2 l

JoJo™-1, Jo-PRo™-1 529 545 XF104-2 l

JRed 584 610 QMaX-Red l

Keima Red 440 620 XF177 l

Kusabira orange 548 559 QMaX-Yellow l

lissamine rhodamine B 570 590 XF102-2 l

lolo™-1, lo-PRo™-1 565 579 XF39 l

lucifer Yellow 428 536 XF14-2 l

lysoSensor™ Blue (pH 5) 374 424 XF131 l

lysoSensor™ green (pH 5) 442 505 XF100-2 l

lysoSensor™ Yellow/Blue (pH 4.2) 384 540 XF129-2 l

lysotracker® green 504 511 XF104-2 l

lysotracker® Red 577 592 QMaX-Red l

lysotracker® Yellow 465 535 XF18-2 l

Mag-Fura-2 330 491 XF04-2 l

Mag-Indo-1 330 417 XF07 l

Magnesium green™ 506 531 XF104-2 l

Marina Blue® 365 460 XF06 l

mBanana 540 553 Call l

mCherry 587 610 QMaX-Red l

mCitrine 516 529 XF104-2 l

4-Methylumbelliferone 360 449 XF06 l

mHoneydew 487 537 Call l

Midorishii Cyan 472 495 XF116-2 l

Mithramycin 395 535 XF14-2 l

Mitofluor Far Red 680 650-773 XF142-2 l

Mitofluor green 490 516 XF100-2 l

Mitofluor Red 589 588 622 XF102-2 l

Mitofluor Red 594 598 630 XF102-2 l

Mitotracker® green 490 516 XF100-2 l

Mitotracker® orange 551 576 XF108-2 l

Mitotracker® Red 578 599 XF102-2 l

Mitotracker® Deep Red 644 655 XF110-2 l

morange 548 562 XF108-2 l

mPlum 590 649 XF110-2 l

mRaspberry 598 625 XF102-2 l

mRFP 584 607 QMaX-Red l



PrODucts

26


fluorophores(s)

mStrawberry 574 596 XF174 l

mtangerine 568 585 XF108-2 l

mtFP 462 492 XF168 l

NBD 465 535 XF18-2 l

Nile Red 549 628 XF103-2 l

oregon green® 488 496 524 XF100-2 l



Pacific Blue™ 410 455 XF119-2 l

PBF1 334 504 XF04-2 l

C-phycocyanin 620 648 XF45 l

R-phycocyanin 618 642 XF102-2 l

R-phycoerythrin (PE) 565 575 XF108-2 l

Phi YFP 525 537 XF104-2 l

PKH26 551 567 XF108-2 l

PoPo™-1, Po-PRo™-1 434 456 XF114-2 l

PoPo™-3, Po-PRo™-3 534 572 XF108-2 l

Propidium Iodide 536 617 XF103-2 l

PyMPo 415 570 XF15 l

Pyrene 345 378 XF02-2 l

Pyronin Y 555 580 XF108-2 l

Qdot™ 525 Conjugate UV 525 XF301-1 Qdot








Quinacrine Mustard 423 503 XF14-2 l

Resorufin 570 585 XF102-2 l

Red Fluorescent Protein (DsRed2) 561 585 XF108-2 l

RH 414 500 635 XF103-2 l

Rhod-2 550 571 XF108-2 l

Rhodamine B 555 580 XF108-2 l

Rhodamine green™ 502 527 XF104-2 l

Rhodamine Red™ 570 590 XF102-2 l

Rhodamine Phalloidin 542 565 XF108-2 l

Rhodamine 110 496 520 XF100-2 l

Rhodamine 123 507 529 XF104-2 l

5-RoX (carboxy-X-rhodamine) 574 602 XF102-2 l

SBFI 334 525 XF04-2 l

Sensilight P-1 550 664 XF36 l

Sensilight P-3 609 661 XF45 l

SItS 337 436 XF03 l

SNaFl®-1 576 635 XF29 Ratio Imaging

SNaFl®-2 525 546 XF29 Ratio Imaging

SNaRF®-1 575 635 XF72 Ratio Imaging

Sodium green™ 507 535 XF104-2 l


fluorophores(s-Z)

Spectrumaqua® 433 480 XF201 MFISH

SpectrumBlue® 400 450 XF06 l

Spectrumgold® 530 555 XF203 MFISH

Spectrumgreen® 497 524 XF202 MFISH

Spectrumorange® 559 588 XF204 MFISH

SpectrumRed® 587 612 XF207 MFISH

SpectrumFRed® 655 675 XF208 MFISH

SYto® 11 508 527 XF104-2 l

SYto® 13 488 509 XF100-2 l

SYto® 17 621 634 XF44 l

SYto® 45 452 484 XF114-2 l

SYtoX® Blue 445 470 XF114-2 l

SYtoX® green 504 523 XF104-2 l

SYtoX® orange 547 570 XF108-2 l

5-taMRa (5-Carboxytetramethylrhodamine) 542 568 XF108-2 l

tdtomato 554 581 XF173 l

tetramethylrhodamine (tRItC) 555 580 XF108-2 l

texas Red®/texas Red®-X 595 615 XF102-2 l

thiadicarbocyanine 651 671 XF47 l

thiazine Red R 510 580 XF28 l

thiazole orange 453 480 XF114-2 l

topaz 514 527 XF104-2 l

t-Sapphire 399 511 XF76 l

toto®-1, to-PRo®-1 514 533 XF104-2 l

toto®-3, to-PRo®-3 642 660 XF110-2 l

to-PRo®-5 748 768 XF143-2 l

turbo RFP 553 574 XF108-2 l

turbo YFP 525 538 XF104-2 l

Venus 515 528 XF104-2 l

WW 781 605 639 XF45 l

X-Rhodamine (XRItC) 580 605 XF102-2 l

YFP (Yellow Fluorescent Protein) 513 527 XF104-2 l

YFP/Cy3® FREt 513 570 XF167 FREt

YFP/tRItC FREt 513 580 XF166 FREt

YoYo®-1, Yo-PRo®-1 491 509 XF100-2 l

YoYo®-3, Yo-PRo®-3 612 631 XF102-2 l

Ypet 517 530 XF104-2 l

Zsgreen1 493 505 XF100-2 l

ZsYellow1 529 539 XF104-2 l






Call for pricing.

27

fluorescent Protein sets Filter sets are arranged by fluorophores and emission wavelength.


Fluorophores Filter Set # Application Components Stock Standard

BFP QMaX-Blue Wide exciter filter may cause cellular damage Exciter QMaX/EX 355-405 l

in live cell applications. Please inquire about Dichroic QMaX/DI 410lP l

excitation to reduce phototoxicity Emitter QMaX/EM 420-480 l

BFP, eBFP XF129-2 longpass emission filter will collect light Exciter XF1075 387aF28 l

from most of all UV excitable sources that Dichroic XF2004 410DRlP l

emit in the visible, including autofluorescence Emitter XF3088 435alP l

BFP, eBFP XF131 Bandpass emission filter Exciter XF1075 387aF28 l

Dichroic XF2004 410DRlP l

Emitter XF3002 450aF65 l

CFP, eCFP, mCFPm, Cerulean, CyPet XF114-2 Bandpass emission filter Exciter XF1071 440aF21 l Dichroic XF2034 455DRlP l Emitter XF3075 480aF30 l

CFP, eCFP, mCFPm, Cerulean, CyPet XF130-2 longpass emission filter is Exciter XF1071 440aF21 l

likely to have higher background Dichroic XF2034 455DRlP l

than bandpass emission filter Emitter XF3087 480alP l

wtGFP XF170 longpass emission filter Exciter XF1076 400aF30 l

uses primary excitation peak Dichroic XF2040 435DRlP l

Emitter XF3087 480alP l

AmCyan XF168 Exciter XF1101 440aF40 l



eGFP, CoralHue Azami Green QMaX-green Exciter QMaX/EX 450-490 l

Emerald Dichroic QMaX/DI 500lP l

Emitter QMaX/EM 510-560 l

CoralHue Midoriishi-Cyan, eGFP XF116-2 For multiple labeling with DsRed2, Exciter XF1072 475aF20 l Fluorescein (FITC) minimizing cellular auto-fluorescence Dichroic XF2037 500DRlP l Alexa Fluor® 488, Cy2® Emitter XF3080 510aF23 l

eGFP XF115-2 longpass emission filter is Exciter XF1073 475aF40 l likely to have higher background Dichroic XF2010 505DRlP l than bandpass emission filter Emitter XF3086 510alP l

wtGFP XF169 Bandpass emission filter Exciter XF1076 400aF30 l

uses primary excitation peak Dichroic XF2040 435DRlP l


CoralHue Azami Green XF171 Exciter XF1102 475aF30 l


Emitter XF3003 520DF40 l

GFP (Sapphire), T-Sapphire, GFPuv XF76 UV Excitation, green Exciter XF1008 405DF40 l

emission color Dichroic XF2006 450DClP l


YFP XF105-2 longpass emission filter is Exciter XF1068 500aF25 l likely to have higher background Dichroic XF2030 525DRlP l than bandpass emission filter Emitter XF3082 530alP l

Emerald XF172 Exciter XF1072 475aF20 l Dichroic XF2077 500DRlP l Emitter XF3084 535aF45 l

ZsGreen1, eGFP, Fluorescein (FITC) XF100-2 Exciter XF1073 475aF40 l Alexa Fluor® 488 Dichroic XF2010 505DRlP l Cy2®, DiO, Fluo-4 Emitter XF3084 535aF45 l

eGFP XF100-3 Exciter XF1087 470aF50 l Dichroic XF2077 500DRlP l Emitter XF3105 545aF75 l

YFP, ZsYellow1 XF104-2 Bandpass emission filter Exciter XF1068 500aF25 l Dichroic XF2030 525DRlP l



Fluorescent Protein Sets

Application High signal-to-noise imaging applications

Design Features alPHa technology excitation and emission filters provide steep edges, increased blocking, and reduced auto-fluorescence

Excitation Filters alPHa bandpass designs

Dichroic Beamsplitters Hard-surface & aR coated

Emission Filters alPHa bandpass/longpass designs

28


PrODuctsFluorescent Protein Sets

Application High signal-to-noise imaging applications

Design Features alPHa technology excitation and emission filters provide steep edges, increased blocking, and reduced auto-fluorescence

Excitation Filters alPHa bandpass designs


Emission Filters alPHa bandpass/longpass designs

fluorescent Protein sets Filter sets are arranged by fluorophores and emission wavelength.



DsRed2, DsRed-Express XF101-2 longpass emission filter is Exciter XF1074 525aF45 • likely to have higher background Dichroic XF2017 560DRlP • than bandpass emission filter Emitter XF3085 565alP •

DsRed2, mTangerine QMaX-Yellow Emission provides a Exciter QMaX/EX 510-560 • yellow-orange color to Ds Red2, Dichroic QMaX/DI 560lP • tRItC and others Emitter QMaX/EM 570-600 •

DsRed2, DsRed-Express XF111-2 longpass emission filter, brighter signal, Exciter XF1077 540aF30 • but potentionally higher background Dichroic XF2015 570DRlP • than bandpass emission filter Emitter XF3089 575alP •

CoralHue Kusabira Orange XF108-2 Bandpass emission filter Exciter XF1074 525aF45 •DsRed2, DsRed-Express Dichroic XF2017 560DRlP •mOrange, mTangerine Emitter XF3083 595aF60 •

tdTomato XF173 Exciter XF1103 535aF30 • Dichroic XF2015 570DRlP • Emitter XF3083 595aF60 •

Hc Red, mCherry, JRed QMaX-Red Red emission and good Exciter QMaX/EX 530-570 • discrimination from egFP in Dichroic QMaX/DI 580lP • co-expression systems Emitter QMaX/EM 600-650 •

AsRed 2, mStrawberry XF174 Exciter XF1104 555aF30 • Dichroic XF2018 580DRlP • Emitter XF3120 610aF50 •

Keima Red XF176 NEW! long pass emission filter Exciter XF1071 440aF21 • to give a rich red color Dichroic XF2019 590DRlP • in single label schemes Emitter XF3094 610alP •

Keima Red XF177 NEW! Same excitation and emission filter as Exciter XF1071 440aF21 • XF176, but blue shifted dichroic Dichroic XF2007 475DClP • mirror, multicolor applications Emitter XF3094 610alP •

Keima Red XF178 NEW! Bandpass version of XF177, Exciter XF1071 440aF21 • best for multi-label applications Dichroic XF2007 475DClP • with other blue excited fluorophores Emitter XF3025 615DF45 •

mCherry XF175 Exciter XF1067 560aF55 • Dichroic XF2020 600DRlP • Emitter XF3081 645aF75 •

HcRed, HcRed1, mRaspberry, MRFP1 XF102-2 Exciter XF1067 560aF55 • Dichroic XF2029 595DRlP • Emitter XF3081 645aF75 •

mPlum XF110-2 Exciter XF1069 630aF50 • Dichroic XF2035 650DRlP • Emitter XF3076 695aF55 •

fluorescent Protein Multi-band sets Filter sets are arranged by fluorophores and emission wavelength.



BFP/eGFP XF50 Dual Band Exciter XF1048 400-500DBEX • Dichroic XF2041 385-502DBDR • Emitter XF3054 460-550DBEM •

CFP/YFP XF135 Dual Band Exciter XF1078 436-510DBEX • Dichroic XF2065 436-510DBDR • Emitter XF3099 475-550DBEM •

eGFP/DsRed2 XF52 Dual Band Exciter XF1050 490-550DBEX • Dichroic XF2043 490-550DBDR • Emitter XF3056 520-580DBEM •

CFP/YFP/DsRed2 XF154 triple Band Exciter XF1100 436-495-582tBEX • Dichroic XF2090 445-510-600tBDR • Emitter XF3118 465-535-640tBEM •


Call for pricing.

fret sets Filter sets are arranged by fluorophores and emission wavelength.

Type Part # DescriptionDonor Acceptor

Fluorophores Filter Set # Components Stock Standard

BFP eGFP XF89-2 Exciter XF1005 365WB50 •

Dichroic XF2001 400DClP • Emitter 1 XF3002 450aF65 • Emitter 2 XF3084 535aF45 •

BFP YFP XF158 Exciter XF1005 365WB50 • Dichroic XF2001 400DClP • Emitter 1 XF3002 450aF65 • Emitter 2 XF3079 535aF26 •

BFP DsRed2 XF159 Exciter XF1005 365WB50 • Dichroic XF2001 400DClP • Emitter 1 XF3002 450aF65 • Emitter 2 XF3019 605DF50 •

CFP YFP XF88-2 Exciter XF1071 440aF21 • Dichroic XF2034 455DRlP • Emitter 1 XF3075 480aF30 • Emitter 2 XF3079 535aF26 •

CFP DsRed2 XF152-2 Exciter XF1071 440aF21 • Dichroic XF2034 455DRlP • Emitter 1 XF3075 480aF30 • Emitter 2 XF3022 580DF30 •

Midoriishi Cyan Kusabira Orange XF160 Exciter XF1071 440aF21 • Dichroic XF2027 485DRlP •

Emitter 1 XF3005 495DF20 •

Emitter 2 XF3302 565WB20 •

eGFP DsRed2 or Rhod-2 XF151-2 Exciter XF1072 475aF20 •

Dichroic XF2077 500DRlP •

Emitter 1 XF3080 510aF23 •


FITC TRITC XF163 Exciter XF1073 475aF40 •




FITC Rhod-2 or Cy3 XF162 Exciter XF1073 475aF40 •




Alexa 488 Alexa 546 or 555 XF164 Exciter XF1087 470aF50 •




Alexa 488 Cy3 XF165 Exciter XF1073 475aF40 •




YFP TRITC or Cy3 XF166 Exciter XF1068 500aF25 •




Cy3 Cy5 or Cy5.5 XF167 Exciter XF1074 525aF45 •




29

For current product listings, specifications, and pricing:www.glenspectra.co.uk . [email protected]+44 020 8204 9517

FRET Sets

Application Determining physical proximity in a sample

Design Features Multiple emission filters

Excitation Filters Bandpass designs


Emission Filters Bandpass designs


APPlIcAtIOn nOte

fIlters & MIcrOscOPe cOnfIGurAtIOns the filter components required for FREt experiments are not esoteric. as in any fluo rescence microscopy application, an excita tion filter is required for exciting the donor fluorophore, and a dichroic mirror is required for separating donor excitation energy from both donor and acceptor emis sion energy. Unlike other fluorescence appli cations, however, two emission filters are required, one for the acceptor fluorophore, or FREt emission, and one for the donor flu orophore in order to correct for SBt. as for choosing specific filters, the same filter com ponents and sets can be applicable for FREt as those which are matched to specific fluo rophores and used in other single color, epi-fluorescence applications.

More important in the selection of filters is an understanding of the physical configura tion of the microscope hardware to be used in the FREt experiment. at issue are critical experimental variables, such as time and image registration. While the ideal set-up may not be affordable or available to all researchers interested in FREt studies, it is nonetheless important to understand the pros and cons of the available hardware and filter set configurations.

1. Multi-View configurations Most ideal for the viewing and measurement of molecular, protein-protein interactions with critical spatial and temporal characteris tic is a set-up which allows for simultaneous viewing of both donor and acceptor emis sion energy. this is only possible using a device which provides a simultaneous split-screen view of the sample. these multi-view accessories are mounted to the microscope in front of the detector and use filters inte grated into the unit to split the donor and acceptor emission fluorescence into two images.

When FRET viewing is handled this way, the two critical variables—timeandregistration—areeliminated.Thetimeofdonorandacceptorimaging is simultaneous, and given a properly aligned unit, the image registra tion is identical, providing a duplicate view of the sample. the only difference between the two images is that one image is captured with an emission filter for donor emission while the other image uses an emission filter for acceptor emission.

2. emission filter WheelsWhen multi-view accessories are not avail able, an automated emission filter wheel is the next best alternative. With this configura tion, a filter cube/holder with a donor excita tion filter and dichroic mirror are placed in the microscope. the emission filters for both the donor and acceptor fluorophores, in turn, are mounted in the emission filter wheel, which can be rapidly switched from one to the other.

Collecting donor and acceptor emission energy using this hardware configuration, while not simultaneous, can be accom plished with time delays of only 40-75msec (depending on make and model), given the state-of-the-art of automated filter wheels as well as camera and detector technology. Both temporal changes in the sample during live cell imaging and registration shift result ing from equipment movement, while almost negated, must still be considered when ana lyzing experimental results.

3. separate filter cubesWithout a multi-view attachment or emission filter wheel, researchers must fall back on a third hardware configuration for FREt, which involves using a separate filter cube for both donor and acceptor fluorescence. In this con figuration, while each cube has an exciter, dichroic and emitter, it is extremely impor tant to remember that the exciter and dichroic in both sets are identical and are those filters that are typically used with the donor fluorophore.

While this third filter configuration allows for the discreet collection of donor and acceptor fluorescence, it is the configuration most susceptible

Optimizing filter sets for fret Applications

OverviewFREt, or Forster Resonance Energy transfer, is a phenomenon where closely matched pairs of fluorophores are used to determine spatial or temporal proximity and specificity in molecular, protein-protein interactions. More specifically, this energy transfer occurs whentheemissionenergyofonefluorophore—thedonor—isnon-radiatively transferred to the secondfluorophore—the acceptor—producing a secondary emission. When this occurs, donor fluores-cence is quenched and acceptor fluorescence increases.

Biologically, in order for this transfer to occur, the cellular conditions need to be such that the distance between the molecules being measured is no more than 1-10nm. Spectrally, the fluorophores being used need to have a large overlap, which while creating the conditions for effective energy transfer, also results in spectral bleed through (SBt), defined as the overlap of the donor and acceptor emission spectra, and can be a problem in FREt measurements.

the development of SBt correction techniques have been critical to the evolution of FREt as a useful and more widely used appli-cation.TheseSBTcorrectiontechniques—whichincludesoftwaredevelopment, fluorescence lifetime imaging (FlIM) correlation, and photobleachingtechniques—havereachedadegreeofsophistica-tion that improves the efficacy of FREt. Similarly, the development of microscopy techniques such as one-photon, two-photon (mul-ti-photon), confocal, and tIRF are all contributing to the growing effectiveness and ease of FREt experiments.

While much has been written about the physical and biological aspects of FREt, as summarized above, this application note will review the best suited fluorophore pairs and summarize the consi-derations surrounding the hardware configuration and selection of optical filters required for successful capture, differentiation, and measurement of FREt.

30


Om

eg

a Op

tical

to the time and image registra tion variables mentioned previously. the time variable can be minimized as a result of the automated turrets, which are a standard feature on many new microscopes but may not be typical on older, installed models. In addition, alignment of filters within cube tol erances allows more room for registration error than in the two other configurations. the time and resolution variables that are inherent with this configuration must be thoughtfully weighed when using a spatially and temporally sensitive technique such as FREt.

fluOrOPHOre PAIrsWhile certain fluorophore pairs such as CFP/YFP, have dominated the scientific litera ture and provided the foundation for suc cessful FREt studies to date, there has been a flurry of activity recently surrounding the development of new monomeric fluorescent proteins which have applicability for FREt experiments. these fluorophore develop ments have been stimulated by the refine ment of procedures and ratio correction techniques, as well as microscopy applica tions that are FREt friendly.

on the most basic level, the success of any given pair of fluorophores centers on their spectral characteristics. First, there must be sufficient separation of excitation spectra for selective stimulation of the donor. Second, there must be sufficient overlap (>30%) between theemission spectrum of the donor and the excitation spectrum of the acceptor in order to obtain efficient energy transfer. and third, there must be sufficient separa tion of the donor and the acceptor emission spectra so that the fluorescence of each fluo rophore can be collected independently.

Development of new fluorescent proteins has centered on meeting these criteria, while producing new colors and fluorophores that bind to varied proteins and biological mole cules. the newest developments are cited in the links and references to recent literature listed below:

Fluorophore References Wallrabe, H., and Periasamy, a. (2005) FREt-FlIM microscopy and spectroscopy in the biomedical sciences. Current opinion in Biotechnology. 16: 19-27. Karasawa, S., araki, t., Nagai, t., Mizuno, H., Miyawaki, a. (2004) Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochemical Journal, april 5. Shaner, N., Campbell, R., Steinbach, P., giepmans, B., Palmer, a., tsien, R. (2004) Improved monomeric red, orange, and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature Biotechnology, Vol. 22, Number 12, December. pp.1567-1572.

fret fIlter setsthe products listed in the catalog include the most commonly used FREt fluorophore pairs, as well as those recently developed pairs that are worthy of attention. For each FREt fluorophore pair the chart lists a filter set that is useful in the emission filter wheel configuration. these sets are comprised of the exciter and dichroic for the donor fluo rophore and emitters for both the donor and acceptor fluorophores.

Individual filter set part numbers for both donor and acceptor fluorophores are also listed so components can be purchased indi vidually, dependent on the specifics of the hardware set-up. If purchasing filters individ-ually, it is important to remember that the exciter and dichroic from the acceptor fluo rophore set are never used.

It is important that you provide hardware details and related mounting instructions when ordering filters for FREt applications

31


PrODuctsQuantum Dot Filters

Application For imaging all UV-excited QDot™ conjugates

Design Features Sets include a choice of two exciters, a single dichroic, and emitters optimized for each QDot

Excitation Filters Wide-band excitation filter for maximum signal or narrower bandpass design to minimize photodamage

Dichroic Beamsplitters Single dichroic functions for all colors

Emission Filters Bandpass designs for high signal-to-noise and discrimination, are aR coated and precision polished

Quantum Dot (Qdot™) filters & filter sets Filter sets are arranged by fluorophores and emission wavelength.



32

Qdot™ All Conjugates XF300 Exciter 1 XF1009 425DF45 l

Exciter 2 XF1301 415WB100 l

Dichroic XF2007 475DClP l

Emitter 1 XF3301 525WB20 l





Emitter 6 XF3113 710aF40 l



Emitter 9 XF3009 625DF20 l

Qdot™ 525 Conjugate XF301-1 Exciter 1 XF1009 425DF45 l

or Exciter 2 XF1301 415WB100 l

XF301-2 (Substitute Exciter 2 for Exciter 1) Dichroic XF2007 475DClP l














Qdot™ 625 Conjugate XF309-1 NEW! Exciter 1 XF1009 425DF45 l


XF309-2 NEW! Dichroic XF2007 475DClP l

(Substitute Exciter 2 for Exciter 1) Emitter 9 XF3009 625DF20 l










For single color or Exciter 2 XF1301 415WB100 l




For multiplexing or Exciter 2 XF1301 415WB100 l





PricingWe provide the most competitive prices. For current product pricing, please visit www.glenspectra.co.uk or contact us at:

[email protected]

+44 020 8204 9517


Call for pricing.

For simultaneous XF320 Exciter XF1009 425DF45 l

multi-color viewing to minimize DAPI Dichroic XF2007 475DClP l


For simultaneous XF02-2 Exciter XF1001 330WB80 l

multi-color viewing with Dichroic XF2001 400DClP l

Xenon excitation Emitter XF3097 400alP l

For simultaneous XF05-2 Exciter XF1005 365WB50 l

multi-color viewing with Hg excitation Dichroic XF2001 400DClP l


Quantum Dot (Qdot™) filters & filter sets



Multi-Q-Dot Emissions

Tran

smis

sion

(%

)

Wavelength (nm)

100 –

90 –

80 –

70 –

60 –

50 –

40 –

30 –

20 –

10 –

0 –

400 600 800 1000

XF1009XF2007XF3301XF3302XF3303XF3304XF3305XF3307

34


PrODuctsPinkel Sets

Application For multi-color high discrimination applica-tions

Excitation Filters Multiple filters must be mounted in a filter wheel or slider

Dichroic Beamsplitters Multi-band designs with multiple passbands and rejection bands

Emission Filters Multi-band designs with multiple passbands and reflection bands

Pinkel sets Filter sets are arranged by fluorophores and emission wavelength.


TRIPLE BAND Exciter 1 XF1006 400DF15 l

DAPI/FITC/Texas Red® XF63-1 Exciter 2 XF1099 485DF20 l

Exciter 3 XF1044 575DF25 l

Dichroic XF2048 400-477-575tBDR l

Emitter XF3061 445-525-650tBEM l

DAPI/FITC/Texas Red® XF67-1 Exciter 1 XF1006 400DF15 l

DAPI/Alexa Fluor® 488/546 Exciter 2 XF1099 485DF20 l

DAPI/Cy2®/Cy3® Exciter 3 XF1044 575DF25 l



DAPI/FITC/TRITC XF68-1 Exciter 1 XF1006 400DF15 l

DAPI/FITC/Cy3® Exciter 2 XF1099 485DF20 l




DAPI/FITC/MitoTracker Red XF69-1 Exciter 1 XF1006 400DF15 l





FITC/Cy3®/Cy5® XF93-1 Exciter 1 XF1099 485DF20 l

FITC/TRITC/Cy5® Exciter 2 XF1043 555DF10 l




CFP/YFP/DsRed2 XF154-1 Exciter 1 XF1201 436aF8 l





DuAL BAND Exciter 1 XF1006 400DF15 l

DAPI/FITC XF50-1 Exciter 2 XF1099 485DF20 l

BFP/eGFP Dichroic XF2041 385-502DBDR l

Emitter XF3054 460-550DBEM l

FITC/TRITC XF52-1 Exciter 1 XF1099 485DF20 l

Cy2®/Cy3® Exciter 2 XF1043 555DF10 l

eGFP/DsRed2 Dichroic XF2043 490-550DBDR l


FITC/Texas Red® XF53-1 Exciter 1 XF1099 485DF20 l


Dichroic XF2044 490-575DBDR l


DAPI/TRITC XF59-1 Exciter 1 XF1094 380aF15 l




CFP/YFP XF135-1 Exciter 1 XF1079 436DF10 l







Call for pricing.

Photoswitchable Protein set



Pinkel sets Continued Filter sets are arranged by fluorophores and emission wavelength.

the set includes seven filters which allows for both green and red visualization, as well as UV conversion. this technique is useful in live cell applications as an optical marking technique for studying cell dynamics.



sedat sets Filter sets are arranged by fluorophores and emission wavelength.



DuAL BAND Exciter 1 XF1042 485DF15 l

FITC/ TRITC XF156 Exciter 2 XF1043 555DF10 l

Dichroic XF2043 490-550DBDRlP l



TRIPLE BAND Exciter 1 XF1006 400DF15 l

DAPI/FITC/TRITC XF157 Exciter 2 XF1009 485DF20 l






QuAD BAND Exciter 1 XF1005 365WB50 l DAPI/FITC/TRITC/Cy5 XF155 Exciter 2 XF1006 400DF15 l

DAPI/FITC/TRITC/ Exciter 3 XF1099 485DF20 l

Alexa Fluor®647 Exciter 4 XF1045 560DF15 l

Exciter 5 XF1208 640aF20 l

Dichroic XF2046 400-485-558-640QBDR l





Green to Red Conversion of XF153 Exciter Red XF1072 475aF20 l

Kaede, Eos, Kikumi Exciter green XF1021 550DF30 l

Conversion - Exciter UV R to g XF1401 380DF30 l Dichroic XF2038 430DRlP l

Conversion - Dichroic UV R to g XF2001 400DClP l Emitter Red XF3017 530DF30 l

Emitter green XF3089 575alP l

QuAD BAND Exciter 1 XF1006 400DF15 l

DAPI/FITC/TRITC/Cy5® XF57-1 Exciter 2 XF1099 485DF20 l



Dichroic XF2046 400-485-558-640QBDR l

Emitter XF3059 460-520-603-710QBEM l

Sedat Sets

Application For multi-color imaging


Dichroic Beamsplitters Stationary dichroic must be mounted in a filter cube.

Emission Filters Multiple single band filters in automated filter wheel

36


PrODuctsM-FISH Sets

Application For maximizing multi-color labeling

Design Features Filter edges are steep and bandwidth is narrow allowing maximization of both emis-sion energy and color discrimination

Excitation Filters Narrow bandpass designs to maximize discrimination

Dichroic Beamsplitters Steep transition designs, hard-surface and aR coated

Emission Filters Narrow bandpass designs to maximize discrimination

DAPI, AMCA, Cascade Blue® XF06 Exciter XF1005 365WB50 l

SpectrumBlue® Dichroic XF2001 400DClP l


DAPI/FITC/Texas Red® XF232 Exciter 1 XF1005 365WB50 l






DAPI/FITC/TRITC XF231 Exciter 1 XF1005 365WB50 l






SpectrumAqua®, CFP, DEAC XF201 Exciter XF1201 436aF8 l



SpectrumGreen®, FITC, EGFP, Cy2® XF202 Exciter XF1202 485aF20 l

Alexa Fluor® 488, Oregon Green® 488 Dichroic XF2010 505DRlP l

Rhodamine GreenTM Emitter XF3017 530DF30 l

SpectrumGold®, Alexa Fluor® 532 XF203 Exciter XF1203 520aF18 l

YFP Dichroic XF2203 545DRlP l


Cy3®, TRITC, Alexa Fluor® 546 XF204 Exciter XF1204 546aF10 l

5-TAMRA, BODIPY® TMR/X Dichroic XF2062 555DRlP l

SpectrumOrange® Emitter XF3022 580DF30 l

Cy3.5® XF206 Exciter XF1206 572aF15 l



SpectrumRed®, Texas Red® XF207 Exciter XF1207 580aF20 l

Alexa Fluor® 568, BODIPY® TR/X Dichroic XF2020 600DRlP l

Alexa Fluor® 594 Emitter XF3028 630DF30 l

Cy5®, BODIPY® 650/665-X XF208 Exciter XF1208 640aF20 l

Alexa Fluor® 647 Dichroic XF2035 650DRlP l


Cy5.5® XF210 Exciter XF1210 665aF32 l



Cy7® XF143-2 Exciter XF1097 730aF45 l



M-fIsH Filter sets are arranged by fluorophores and emission wavelength.





Call for pricing.

SINGLE DYE EXCITATION SETS Exciter 1 XF1093 340aF15 l

Fura-2, Mag-Fura-2 XF04-2 Exciter 2 XF1094 380aF15 l

PBFI, SBFI Dichroic XF2002 415DClP l

Emitter XF3043 510WB40 l

BCECF XF16 Exciter 1 XF1071 440aF21 l


Dichroic XF2058 515DRlPXR l


Fura Red™ XF17 Exciter 1 XF1071 440aF21 l


Dichroic XF2009 550DClP l


SNAFL®-1, SNAFL®-2 XF29 Exciter 1 XF1080 510DF25 l




BTC XF133 Exciter 1 XF1006 400DF15 l




ratio Imaging sets Filter sets are arranged by fluorophores and emission wavelength.



SINGLE DYE EMISSION SETS Exciter XF1004 355Ht15 l

Indo-1, Mag-Indo-1 XF07 Dichroic 1 XF2003 390DRlP l

LysoSensor™ Yellow/Blue (pH 4.2) Dichroic 2 XF2006 450DClP l



BODIPY® FL-Ceramide, JC-1 XF26 Exciter XF1099 485DF20 l


Emitter 1 XF3016 0g590 l


SNARF®-1 XF31 Exciter XF1018 515NB2.0 l

Confocal Dichroic 1 XF2013 540DClP l

Dichroic 2 XF2014 610DRlP l



SNARF®-1 XF72 Exciter XF1080 510DF25 l

Widefield Dichroic 1 XF2013 540DClP l

Dichroic 2 XF2014 610DRlP l



GeneBLAzer™ (CCF2) XF124 Exciter XF1006 400DF15 l




DuAL DYE SET Exciter 1 XF1093 340aF15 l

Fura-2/BCECF XF79-2 Exciter 2 XF1094 380aF15 l

Exciter 3 XF1071 440aF21 l


Dichroic XF2031 505DRlPXR l


Ratio Imaging Sets

Application Ratiometric measurements of dyes

Design Features Multiple excitation and multiple emission filter sets

Excitation Filters Bandpass designs

Dichroic Beamsplitters Hard-surface and aR coated

Emission Filters Bandpass designs

38


PrODuctsMultiband Sets

Application Simultaneous viewing of multiple fluorophores

Design Features Dual, triple & quad bandpass designs

Excitation & Emission Filters, as well as Dichroic Beamsplitters are multi-band designs with multiple passbands and rejection bands

Multi-band sets Filter sets are arranged by fluorophores and emission wavelength.



DuAL BAND Exciter XF1048 400-500DBEX l

DAPI/FITC XF50 Dichroic XF2041 385-502DBDR l

BFP/eGFP Emitter XF3054 460-550DBEM l

DAPI/TRITC XF59 Exciter XF1054 390-520DBEX l



CFP/YFP XF135 Exciter XF1078 436-510DBEX l



FITC/TRITC XF52 Exciter XF1050 490-550DBEX l

eGFP/DsRed2 Dichroic XF2043 490-550DBDR l


FITC/APC XF51 Exciter XF1049 490-605DBEX l



FITC/Texas Red® XF53 Exciter XF1051 490-577DBEX l



Cy3®/Cy5® XF92 Exciter XF1062 550-640DBEX l



TRIPLE BAND Real time visual detection Exciter XF1055 400-477-580tBEX l

DAPI/FITC/Texas Red® XF63 Dichroic XF2048 400-477-575tBDR l


DAPI/FITC/Texas Red® XF56 Real time visual imaging with Exciter XF1052 390-486-577tBEX l

a CCD camera or color film Dichroic XF2045 400-485-580tBDR l


DAPI/FITC/Texas Red® XF67 Real time visual detection Exciter XF1058 390-486-577tBEX l



DAPI/FITC/TRITC XF66 Real time visual imaging with Exciter XF1057 385-485-560tBEX l

a CCD camera or color film Dichroic XF2050 385-485-560tBDR l


DAPI/FITC/TRITC XF68 Real time visual detection Exciter XF1059 386-485-560tBEX l



DAPI/FITC/Cy5® XF95 Exciter XF1065 400-510-630tBEX l



DAPI/FITC/Propidium Iodide XF69 Exciter XF1098 400-495-575tBEX l



DAPI/Cy3®/Cy5® XF94 Exciter XF1064 415-535-630tBEX l



CFP/YFP/DsRed2 XF154 Exciter XF1100 436-495-582tBEX l



FITC/Cy3®/Cy5® XF93 Exciter XF1063 485-555-650tBEX l



QuAD BAND Exciter XF1053 405-490-555-650QBEX l

DAPI/FITC/TRITC/Cy5® XF57 Dichroic XF2046 400-485-558-640QBDR l

DAPI/FITC/TRITC/ Alexa Fluor®647 Emitter XF3059 460-520-603-710QBEM l





Call for pricing.

emission color chart350 400 450 500 550 600 650 700 750

350 400 450 500 550 600 650 700 750

DuAL BAND

XF50

XF59

XF135

XF52

XF51

XF53

XF92

TRIPLE BAND

XF63

XF56

XF67

XF66

XF68

XF95

XF69

XF94

XF154

XF93

QuAD BAND

XF57

excitation emission

40


PrODuctsCaged Compound Sets

Application UV excitation uncages fluorophores for controlled viewing


Dichroic Beamsplitters Extended reflection design. Dichroic beamsplitters must be mounted in a filter holder

Emission Filters Emission must be mounted in a filter holder

caged compound sets Filter sets are arranged by fluorophores and emission wavelength.

available in standard dichroic sizes and designed to function at 45 degree angle of incidence from 400–700nm.

For reducing excitation energy.



Multiphoton filters are used in conjunction with two- and three-photon IR laser excitation of fluoro-phores for imaging deeper into samples with minimal photobleaching and photodamage to cells.Multi-Photon filters

Components Stock Standard


Dichroic XF2033 675DCSPXR l laser Blocking Filter XF3100 710aSP l

beamsplitters & MirrorsPart # Description Application Size

XF121 50/50 Beamsplitter 50%t, 50%R Standard dichroic XF122 70/30 Beamsplitter 70%t, 30%R Standard dichroicXF123 30/70 Beamsplitter 30%t, 70%R Standard dichroicXF125 ReflectingMirror Opaquebackingpreventstransmission,>=90%Reflection Standarddichroic

neutral Density filtersSizesApertures

Description Part #

ND 0.05 XB193/18R XB193/25R XB193/32R XB193/45R XB193/50R XB193/50S ND 0.1* XB13/18R XB13/25R XB13/32R XB13/45R XB13/50R XB13/50S ND 0.2 XB194/18R XB194/25R XB194/32R XB194/45R XB194/50R XB194/50S ND 0.3* XB14/18R XB14/25R XB14/32R XB14/45R XB14/50R XB14/50S ND 0.4 XB195/18R XB195/25R XB195/32R XB195/45R XB195/50R XB195/50S ND 0.5* XB15/18R XB15/25R XB15/32R XB15/45R XB15/50R XB15/50S ND 0.6 XB196/18R XB196/25R XB196/32R XB196/45R XB196/50R XB196/50S ND 0.7 XB197/18R XB197/25R XB197/32R XB197/45R XB197/50R XB197/50S ND 0.8 XB198/18R XB198/25R XB198/32R XB198/45R XB198/50R XB198/50S ND 1.0* XB16/18R XB16/25R XB16/32R XB16/45R XB16/50R XB16/50S ND 2.0* XB17/18R XB17/25R XB17/32R XB17/45R XB17/50R XB17/50S ND 3.0* XB27/18R XB27/25R XB27/32R XB27/45R XB27/50R XB27/50S Set of 6 - includes items with * XB28/18R XB28/25R XB28/32R XB28/45R XB28/50R XB28/50S Set of 12 XB199/18R XB199/25R XB199/32R XB199/45R XB199/50R XB199/50S

18Ø 25Ø 32Ø 45Ø 50Ø 50 x 50

Apertures: 13Ø 20Ø 27Ø 40Ø 45Ø 45 x 45

FITC XF73-2 Uncaging Exciter XF1001 330WB80 l Dye Exciter XF1073 475aF40 l Dichroic XF2031 505DRlPXR l Dye Emitter XF3084 535aF45 l

TRITC XF74-2 Uncaging Exciter XF1001 330WB80 l Dye Exciter XF1074 525aF45 l Dichroic XF2032 565DRlPXR l Dye Emitter XF3085 565alP l

FITC/TRITC XF75 Uncaging Exciter XF1001 330WB80 l Dye Exciter XF1050 490-550DBEX l Dichroic XF2039 485-555DBDR l Dye Emitter XF3056 520-580DBEM l

EXTENDED REFLECTION DICHROIC ONLY FITC Extended Reflection Dichroic XF2031 505DRlPXR l

TRITC Extended Reflection Dichroic XF2032 565DRlPXR l

FITC/TRITC Dual Dichroic with UV Reflection XF2039 485-555DBDR l





Call for pricing.

Ir blocking filtersPart # Description Application Typical T% Size

XF83 Kg5 Blocks infrared energy at the light source 80% avg. 12R, 18R, 20R, 22R, 25R 32R, 45R, 50R, 50S

XF85 550CFSP 99+%NearIRAttenuation,600-1200nm >75%T 12R,18R,20R,22R,25R 32R, 45R, 50R, 50S

XF86 700CFSP 99+%NearIRAttenuation,750-1100nm >90%T 12R,18R,20R,22R,25R 32R, 45R, 50R, 50S

Ir-DIc filtersPart # Description Application Size

XF117/32R 780DF35 Capture of fluorescence and infrared DIC images 32mm

XF117/45R 780DF35 Capture of fluorescence and infrared DIC images 45mm

Polarizing filtersPart # Description Application Size

XF120 Polarizing Filter Polarize light in both the excitation and emission path 50 x 50 mm

fluorescence reference slidesPart # Set of 4 slides Description

XF900 Blue emission DaPI/Indo-1/Fura

green emission FItC/gFP

Yellow emission acridine orange

Red emission Rhodamine/texas Red®

Used to reduce infrared energy from the light source in the excitation path (XF83) or in front of the detector in the emission path (XF85 and XF86).

Used for simultaneous capture of fluorescence and infrared DIC images.

Used to polarize incident light in both the excitation and emission path.

this set of slides helps to: center and adjust the fluorescence illuminator; verify uniformity of fluorescence staining; monitor and adjust laser output and PMt settings; and avoid microspheres and photobleaching.

42


PrODucts Microscope Cubes and Filter Holders

omega optical offers holders or cubes for the most common fluorescence microscopes offered by Nikon, olympus, leica, and Zeiss. We can mount filters either in a factory sup-plied holder or a customer supplied holder.

Microscope cubes and filter Holders Type

Exciter Dichroic Emitter

Nikon XC100 original (labophot, Diaphot, optiphot, Microphot, tMD, FXa) 18mm 18 x 26mm 18mm

XC101 Modified (labophot, Diaphot, optiphot, Microphot) 20mm 18 x 26mm 22mm

XC102 Quadfluor, Eclipse (E Models; tE 200/300/800; lV 150/150a/100D, Diaphot 200 & 300, labophot 2 and alphaphot 2 ) 25mm 25.7 x 36mm 25mm

XC104 tE2000, Eclipse 50i, 80i, lV- series 25mm 25.7 x 36mm 25mm

XC106 tE2000 plastic cube, compatible with aZ100 25mm 25.7 x 36mm 25mm

Olympus XC110 IMt-2 22mm 21 x 29mm 20mm

XC111 BH2(cubestyle—notbarrel,BHT,BHS,BHTU,AHBS3,AHBT3) 18mm 18x26mm 18mm

XC113 BX2 (BX, IX, aX) 25mm 25.7 x 36mm 25mm

XC114 CK-40 (CK Models 31/40/41, CB Models 40/41, CKX 31/41) 20mm 21 x 29mm 20mm

XC115 SZX-Fl Stereo Exciter Holder (Holds 1 exciter filter) 25mm N/a N/a

XC116 SZX-Fl Stereo Emission Holder (Holds 2 emission filters) N/a N/a 20mm

Leica XC120 Ploemopak (DMIl, Diaplan, Dialux, Diavert, Fluovert, labolux, labovert, orthoplan, ortholux) 18mm 18 x 26mm 18mm XC121 DM (DMl, DMR, DMlB, DMlM, DMlFS, DMlP) 22mm 21 x 29mm 22mm XC122 DMIRB (DMIl, DMRXa2, DMlS, DMICHB, DMlSP) 20mm 18 x 26mm 20mm XC123 DM2000, DM2500, DM3000, DM4000, DM5000, DM6000 22mm 21 x 29mm 22mm XC124 MZ Fl III Stereo (Holds 2 emission filters) 18mm N/a 18mm

Zeiss XC131 axio Exciter Slider (for exciters or ND filters, 5 ports) 18mm N/a N/a XC132 axioskop 2 Cube (axioplan 2, axioskop 2, axiovert 25, axioskop 2FS) 25mm 25.7 x 36mm 25mm XC133 axiovert 3Fl Slider 25mm 25.7 x 36mm 25mm XC134 axioskop 4Fl Slider (axiovert 100/135, axioplan 1, axioskop 1, axioskop FS 1) 25mm 25.7 x 36mm 25mm XC135 axioskop 6Fl Slider (axiovert 100/135, axioplan 1, axioskop 1, axioskop FS 1) 25mm 25.7 x 36mm 25mm XC136 axio 2 Push-and-Click 25mm 25.7 x 36mm 25mm XC137 axioskop 5Fl Slider (axiovert 100/135, axioplan 1, axioskop 1, axioskop FS 1) 25mm 25.7 x 36mm 25mm XC138 axioskop 8Fl Slider 25mm 25.7 x 36mm 25mm XC139 Standard 2Fl Slider (axiovert 100/135, axioplan 1, axioskop 1, axioskop FS 1) 25mm 25.7 x 36mm 25mm

Zeiss XC132

Olympus XC113 Leica XC122Leica XC121Olympus XC111Nikon XC104

Zeiss XC136

Nikon XC106

Zeiss XC131

Leica XC123

Manufacturer & ModelOmega Optical Part #

filters for flow cytometry

For current product listings, specifications, and pricing:www.glenspectra.co.uk . [email protected]+44 020 8204 9517 43

Figure 1A

Dual Excitation Wavelengths514 nm + 413 nm

YFP

CFP CFP

YFP

DsRedDsRed

GFP

510/20

525 SP

550/30

610 SP

3-waysplit

mirror

3-waysplit

mirror

470/20

610/20

560 SP

514 nm primary

413 nm tertiary

488 nm primary

407 nm tertiary

470/20

546/20

610/20

YFP

CFP CFP

YFP

DsRedDsRed

GFP

510/20

525 SP

550/30

610 SP

3-waysplit

mirror

3-waysplit

mirror

470/20

610/20

560 SP

514 nm primary

413 nm tertiary

488 nm primary

407 nm tertiary

470/20

546/20

610/20

Figure 1B

Dual Excitation Wavelengths488 nm + 407 nm

omega optical offers flow cytometry filters to support a wide variety of fluorophores, tandems, and multi-color cell sorting and analysis applications. to meet the demands of the increasing number of lasers and fluorescent detection channels offered by current flow cytometers, the filters are designed for maximum multiple dye discrimination.

Filters designed for use in flow cytometry must meet performance specifications suitable for laser applications. Dichroic mirrors used for beam directing/splitting are made of durable, hard, or semi-hard coatings for long lifetimes, and substrates can be ground and polished to meet parallelism specifications. Emission filters have precisely placed transmission bands and are designed to attenuate energy at the excitation laser line to optical density (oD) 5 or better.

We work with researchers in the flow cytometry community to continually provide optimal filter selections for both standard methodologies and customized set-ups. often the default filters supplied with the instrument are not optimal for discriminating between closely matched fluorophores, and a novel filter arrangement is required for the separation.

For instance, three or four fluorescent proteins can be simultaneously detected using two lasers for excitation (407 and 488nm, or 413 and 514nm) and several narrow band discriminating filters for emission.1

See Figure 1 for filter set up for three (a) and four (B) fluorescent protein detection.

1 Methods in Molecular Biology: Flow Cytometry Protocols, 2nd ed. Chp 12. Edited by Hawley, T.S and Hawley, R.G. Humana Press Inc. 2004.


PrODucts

Dyes Part # Description

355 Indo-1 (bound Ca 2+) XCY-390DF30Indo-1 (bound Ca 2+) XCY-405DF20DaPI, aMCa, Hoechst 33342 and 32580, alexa Fluor® 350, Marina Blue® XCY-424DF44

405 Cascade Blue®, Pacific Blue™, Hoechst 34580 XCY-440DF10

Cascade Blue®, Pacific Blue™ NEW! XCY-440DF40

Wider emission band for greater signal.

DaPI, aMCa, Hoechst 33342 and 32580, alexa Fluor® 350, Marina Blue ®, BFP

XCY-450DF20

alexa Fluor® 405, Pacific Blue™ XCY-450DF50Chromomycin a3 XCY-475aBlPIndo-1 (free Ca+2), CFP XCY-485DF22Indo-1 (free Ca+2), CFP XCY-495DF20

Pacific orange NEW! XCY-550DF40

Filter created under collaboration with Dr. tomas Kalina, Charles University, Prague.

405/488 Quantum Dot Emission Filters the 405 laser is optimal for excitation of Quantum Dots, but the 488 line laser can also be used.Qdot 525 XF3301 525WB20Qdot 565 XF3302 565WB20Qdot 585 XF3303 585WB20Qdot 605 XF3304 605WB20Qdot 655 XF3305 655WB20Qdot 705 XF3113 715aF40

488 gFP (for separation from YFP, also for separation from Qdots 545 and higher) XCY-510DF21gFP, FItC, alexa Fluor® 488, oregon green® 488, Cy2®, ElF®-97, PKH2, PKH67, Fluo3/Fluo4, lIVE/DEaD Fixable Dead Cell Stain

XCY-530DF30

gFP, FItC, alexa Fluor® 488, oregon green® 488, Cy2®, ElF-97, PKH2, PKH67, YFP

XCY-535DF45

YFP (for separation from gFP) XCY-550DF30

488 or 532 PE, PI, Cy3®, CF-3, CF-4, tRItC, PKH26 XCY-575DF26Mithramycin XCY-575aBlPPE, PI, Cy3®, CF-3, CF-4, tRItC, PKH26 XCY-585DF22lissamine Rhodamine B, Rhodamine Red™, alexa Fluor® 568, RPE-texas Red®, live/Dead Fixable Red Stain

XCY-610DF20

lissamine Rhodamine B, Rhodamine Red™, alexa Fluor® 568, RPE-texas Red®, live/Dead Fixable Red Stain

XCY-610DF30

RPE-texas Red®, texas Red®, alexa Fluor® 594 XCY-630DF22PE-alexa Fluor® 610 XCY-635DF40PE-Cy5® NEW! XCY-660DF35PE-Cy5®, PE-Cy5.5®, PE-Cy7® XCY-680aglP

532 PE-Cy5.5®, PE-alexa Fluor® 700 NEW! XCY-710DF40

633 aPC, alexa Fluor® 633, CF-1, CF-2, PBXl-1, PBXl-3 XCY-660DF20

Cy5® (for separation from Cy7® and conjugates) XCY-670DF40Cy5®, alexa Fluor® 647, to-PRo-3 XCY-675DF20

488 or 633 Cy5®, alexa Fluor® 647, alexa Fluor® 660, PE-alexa Fluor® 647, PE-Cy5®, to-PRo-3

XCY-682DF22

Cy5.5®, alexa Fluor® 680, PE-alexa Fluor® 680, aPC-alexa Fluor® 680, PE-Cy5.5®

XCY-710DF20

633 aPC-Cy5.5® XCY-700DF20Cy7® (for separation from Cy5® and conjugates) XCY-740aBlPPE-Cy7®, aPC-Cy7® XCY-748alPCy7® XCY-770aBlPCy7®, aPC-alexa Fluor® 750 XCY-787DF43

r

ed

B

lu

e

vio

le

t

ul

tr

Av

iol

et

excitAtionlAser

green

flow cytometry emission filters

44

Flow Cytometry Filters

Dichroic Beamsplitters Size: 25, 15, and 12.5 mm. Shape: Specify round and/or square. thickness: Ring 6.7 mm aoI: Specify dichroic aoI 45° or 11.25°

Emission Filters Size: 25, 15, and 12.5 mm. Shape: Specify round and/or square. thickness: Ring 6.7 mm Blocking: UV-900nm, oD5





Call for pricing.

APPlIcAtIOn nOtetesting the optimal filters for Pacific Orange conjugates in polychromatic flow cytometry experiments.

Flow Cytometry Filter SetS

tomas Kalina, ClIP – CytometryDepartment of Paediatric Hematology/oncology2nd Medical SchoolCharles University Prague, Czech Republic

rAtIOnAleCurrently used polychromatic flow cytometers (BD lSR2, Dako Cyan aDP, BD Canto 2) are equipped with two detectors for 405 nm laser excited fluorochromes. BD recommends using Pacific Blue and amCyan conjugates, whereas Dako recom-mends Pacific Blue and Cascade Yellow conjugates for respective instruments.

Both dyes for the 2nd Violet channel have their drawbacks: amCyan dye, while the brightest, has significant spillover to the Pacific Blue, FItC and PE channel, thus after appropriate compensation it creates the signal of negative events. Cascade Yellow has no such unwanted spillover but is relatively dimmer than alternative dye Pacific oran-ge (Molecular Probes/Invitrogen). Based on previous comparison tests we selected Pacific orange dye as a second violet dye for our nine (9) color experiments.

Pacific orange has peak emission at 551 nm. But the emission curve is rather wide so it can be measured in range of wavelengths from 510 nm to 620 nm. We initially found the detection at 585/42 nm slightly superior to 525/50 nm, mainly due to lower background. Surprisingly, we detected contaminating signal from FItC and from PE.

MetHODsWe measured single stained tubes with CD4 and CD8 Pacific orange to test signal/noise ratio of Pacific orange stained peripheral blood cells in following standard emis-sion filters 525/50, 585/42 and compared them to newly designed emission filters 550/20, 550/30, 550/40 by omega optical, Inc.

suMMAry For the BD lSR2 instrument we found the 550/40 filter to be the best compromise solution. It eliminates a negative impact of spillover from Pacific Blue and minimizes the spillover from FItC and PE, while still improving the signal/background ratio over the default 525/50 filter.

For the Dako Cyan aDP instrument we evaluated more limited number of samples and did not find any improvement over the 530/40 filter used by default.

flow cytometry Dichroic filters

Part # DescriptionXCY-380DRLP Detection of side scatter during Indo-1 analysis

XCY-425DRLP Separation of DaPI from FItC and gFP

XCY-450DRLP Separation of Cascade Blue® and Pacific Blue™ from FItC

XCY-460DRLPXR Extended Reflection longpass; Indo-1 ratiometric analysis (extended

reflection) and Separation of Hoechst 33258 from Chrononycin a3

XCY-480DRLP

Separation of Cascade/Pacific Blue from Qdots and longer wavelength

dyes to 700nm

Separation of Cascade Blue® from Cascade Yellow® of ElF®-97

XCY-505DRLPXR Extended Reflection longpass; Reflects 451nm, 457nm, 477nm, 488nm

andUVlaserlines,Transmits>525nm

XCY-525DCSP Shortpass; Separation of gFP from YFP

XCY-560DRSP Shortpass; Separation of FItC from PE

XCY-570DRLP Separation of Mithramycin from Ethidium Bromide

XCY-610DRSP Shortpass; Separation of FItC or PE from PE-Cy5®; Hoechst SP analysis

NEW! XCY-630DRLP Separation of PE-Cy5® from Cy5.5® and Cy7® and their conjugates

XCY-640DRLP Separation of aPC from dyes with shorter wavelength

NEW! XCY-680DRLP Separation of PE-Cy5® and PE-Cy5.5

XCY-690DRLP Separation of aPC from aPC-Cy5.5® or aPC-Cy7®

XCY-710DMLP Separation of PE and Cy5® from PE-Cy5.5® or PE-Cy7®

XCY-760DRLP Separation of Cy5.5® from Cy7® and their conjugates

Filter Set # Laser Fluorophores Type Components

NEW!XCY-502

405

457 CFP/YFP

Emitter 1

Emitter 1

Emitter 2

Dichroic

XCY-470DF20

XCY-485DF22

XCY-550DF30

XCY-505DRlPXR

NEW! XCY-503

457 CFP/gFP/YFP

Emitter 1

Emitter 2

Emitter 3

XCY-485DF22

XCY-510DF21

XCY-550DF30

XFY-500 488 EgFP/YFP

Emitter 1

Emitter 1

Dichroic

XCY-510DF21

XCY-550DF30

XCY-525DCSP

NEW! XCY-504

488 gFP/YFP/DsRed

Emitter 1

Emitter 2

Emitter 3

XCY-510DF21

XCY-550DF30

XCY-610DF20

NEW! XCY-505

488gFP/YFP

DsRed/DsRed2

Emitter 1

Emitter 2

Dichroic

XCY-530DF30

XCY-610DF20

XCY-560DRSP

XCY-501 633 Cy5®/Cy7®

Emitter 1

Emitter 2

Dichroic

XCY-670DF40

XCY-740aBlP

XCY-710DMlP

46


APPenDIX

Helium-neon 543 594 633

200nm 300 400 500 600 700

argon/Krypton 346 351 457 476 488 514 647 676

200nm 300 400 500 600 700

argon Ion 346 351 457 476 488 514

200nm 300 400 500 600 700

Detectors

leD

300nm 400 500 600 700 800 900

Metal Halide

Mercury 313 334 365 405 436 546 577

Xenon

200nm 300 400 500 600 700

200nm 300 400 500 600 700 800 900 1000 1100

lasers

Arc lamps

exfo™

Relative spectral response of the commonly used detectors: photopic human eye, scotopic human eye and color film.

Yellow DyeScotopic Eye ResponseMagenta DyePhotopic Eye ResponseCyan Dye

100

10-2

10-1

350nm 450 550 650 750

Normalized response of bi-alkali PMt detector, extended red PMt detector, silicon detector, S20 PMt and CCD.

Bi-alkali PMtS20 PMtExtended Red PMtCCDSi Photodiode

100

10-2

10-1

100

10-2

10-1

100

50

0 350nm 450 550 650 750

LAM

sFL

uO

RO

PH

OR

ES

PE

CTR

uM

Nor

mal

ised

375 400 425 450 475 500 525 550 575 600 625 650 675

400244-3403

445244-3406

465244-3402

505244-3405

535244-3410

585244-3412

490244-3413

525244-3401

565244-3411

595244-3407

635244-3408

precisExcite LED optionsDAPI GFP Alexa594

Hoechst FITC mCherry

Pacific Blue Alexa488 Texas Red

Cy3.5CFP

YFP Cy5Cy3

TRITC

mRFP

General Information

For current product listings, specifications, and pricing:www.glenspectra.co.uk �. [email protected] +44 020 8204 9517

4

Filter Types and Technology

QuantaMAX: omega optical’s QuantaMaX filters are manufactured using state-of-the-art Energetic Process coating technology for high durability, and low temperature sensitivity. these high throughput filters provide excellent results in all applications. they are especially effective in applications where photons are scarce and exposure times are critical. QuantaMaX filter designs consider not just peak filter transmission and fluorophore probability curves, but also optimized band placement in relation to light sources, and blocking in relation to detector sensitivity.

ALPHA Technology: omega optical’s proprietary coating process produces extremely steep slopes and precise edge location of cut-on and cut-off wavelengths. In addition, it provides the ability to place transmission and rejection regions exceptionally close together, and high attenuation between the passband and the rejection band.

3rd Millennium Technology: the most critical feature of filter design is the transition from blocking to transmission. Precise location of band edges, coupled with steep slopes, allows for optimal transmission of spectral energy. 3rd Millennium filters are specified by the critical cut-on and cut-off edges, resulting in much more accurate band placement and bandwidth, compared to industry standard filters that are defined by a Center Wavelength (CWl) and a Bandwidth (FWHM). Multi-band Filters: omega optical is a co-holder of the patent on all multi-band filters; including dual-band, triple-band, and quad-band designs.

Others:


For optimum instrument system performance we recommend collaborative engineering for custom filter solutions. omega optical is a leading supplier for fluorescence based instruments and applications including: Microplate Readers, DNa Sequencers & analyzers; gene Chip Readers; Flow Cytometers and Cell Sorters; Real time PCR analyzers; Scanners and Imaging Systems; High-throughput and High-Content Systems; Fluorescence Microscopes, and others.

Custom Filters – Small Quantities

Custom filters in single or small quantities are available to ship in 5 – 10 days from our extensive inventory of components. Visit Build-a-Filter at www.omegafilters.com

Aftermarket filters

If you are in need of a filter that did not come with your original equipment, please go to our Build-A-Filter tool on our website. Select your instrument, tell us your filter wave length, or cut-on or cut-off, and we do the rest. No need to specify physical dimensions. See page 47.

Bandpass FiltersEdge FiltersDichroic MirrorsBeamsplitters

Full MirrorsNeutral Density FiltersRejection Band FiltersColor Correction Filters

anti-Reflective Coatingslongpass FiltersShortpass FiltersHot/Cold Mirrors

Wideband FiltersMulti-band FiltersNarrow-band Filters

Filters Fluorescence and Life Science · PDF fileGeneral Information. For current product...

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