Figure 7.1 The Biology of Cancer (© Garland Science 2007)

What’s dominant?

Cell Fusion Allows Interaction Of Disparate Genomes

Retinoblastoma strikes in childhood and is easily diagnosed.

RETINOBLASTOMA

Figure 7.5a The Biology of Cancer (© Garland Science 2007)

Figure 7.5b The Biology of Cancer (© Garland Science 2007)

Familial vs sporadic Retinoblastoma Retinoblastoma Pedigree

Figure 7.8 The Biology of Cancer (© Garland Science 2007)

Heterozygosity – 2 ways of Losing it

Mitotic

Recombination

Gene

Conversion

Figure 7.13 The Biology of Cancer (© Garland Science 2007)

Testing For Loss of Heterozygosity

By Southern Blot By PCR

Figure 7.14 The Biology of Cancer (© Garland Science 2007)

Figure 7.16 The Biology of Cancer (© Garland Science 2007)

Losing Heterozygosity – A Third Way

Methylation

An example

Figure 7.19 The Biology of Cancer (© Garland Science 2007)

Many Tumor Suppressors are Silenced by Hypermethylation

Figure 7.21 The Biology of Cancer (© Garland Science 2007)

NF-1 – Neurofibromatotosis -1 is a GTPase Activating Protein

Figure 7.24a The Biology of Cancer (© Garland Science 2007)

APC-/-APC+/- or +/+

APC, β-Catenin & Intestinal Crypts

Figure 7.25a The Biology of Cancer (© Garland Science 2007)

Figure 7.28a The Biology of Cancer (© Garland Science 2007)

The Von Hippel Landau tumor Suppressor and HIF-1

Figure 7.29b The Biology of Cancer (© Garland Science 2007)