Characterisation of coral explants: a model organism for ...
Fig. 1. Kanamycin concentration in mg l -1 a % Regeneration inhibition Maximum number of GUS foci /...
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Transcript of Fig. 1. Kanamycin concentration in mg l -1 a % Regeneration inhibition Maximum number of GUS foci /...
![Page 1: Fig. 1. Kanamycin concentration in mg l -1 a % Regeneration inhibition Maximum number of GUS foci / explant Portion of Leaf % Regenerating explants b.](https://reader035.fdocuments.net/reader035/viewer/2022070416/5697c0201a28abf838cd1fe1/html5/thumbnails/1.jpg)
Fig. 1
![Page 2: Fig. 1. Kanamycin concentration in mg l -1 a % Regeneration inhibition Maximum number of GUS foci / explant Portion of Leaf % Regenerating explants b.](https://reader035.fdocuments.net/reader035/viewer/2022070416/5697c0201a28abf838cd1fe1/html5/thumbnails/2.jpg)
Kanamycin concentration in mg l-1a
% R
egen
erat
ion
inh
ibit
ion
Max
imu
m n
um
ber
of
GU
S f
oci
/ ex
pla
nt
Portion of Leaf
% R
egen
erat
ing
exp
lan
ts
b
Fig. 2
% Regenerating explantsMaximum number of GUS foci / explant
![Page 3: Fig. 1. Kanamycin concentration in mg l -1 a % Regeneration inhibition Maximum number of GUS foci / explant Portion of Leaf % Regenerating explants b.](https://reader035.fdocuments.net/reader035/viewer/2022070416/5697c0201a28abf838cd1fe1/html5/thumbnails/3.jpg)
a b c d
Fig. 3
![Page 4: Fig. 1. Kanamycin concentration in mg l -1 a % Regeneration inhibition Maximum number of GUS foci / explant Portion of Leaf % Regenerating explants b.](https://reader035.fdocuments.net/reader035/viewer/2022070416/5697c0201a28abf838cd1fe1/html5/thumbnails/4.jpg)
d
% R
egen
erat
ing
exp
lan
ts
Max
imu
m n
um
ber
of
GU
S f
oci
/ ex
pla
nt
Co-cultivation duration in daysc
% R
egen
erat
ing
exp
lan
ts
Max
imu
m n
um
ber
of
GU
S f
oci
/ ex
pla
nt
Acetosyringone concentration in µM
a
% R
egen
erat
ing
exp
lan
ts
Max
imu
m n
um
ber
of
GU
S f
oci
/ ex
pla
nt
Bacterial Density at OD600
b
% R
egen
erat
ing
exp
lan
ts
Max
imu
m n
um
ber
of
GU
S f
oci
/ ex
pla
nt
Co-inoculation duration in minutes
Fig. 4
% Regenerating explantsMaximum number of GUS foci / explant
% Regenerating explantsMaximum number of GUS foci / explant
% Regenerating explantsMaximum number of GUS foci / explant% Regenerating explants
Maximum number of GUS foci / explant
![Page 5: Fig. 1. Kanamycin concentration in mg l -1 a % Regeneration inhibition Maximum number of GUS foci / explant Portion of Leaf % Regenerating explants b.](https://reader035.fdocuments.net/reader035/viewer/2022070416/5697c0201a28abf838cd1fe1/html5/thumbnails/5.jpg)
Fig. 5
ba c d
j
k
h if g
e
l m n o
![Page 6: Fig. 1. Kanamycin concentration in mg l -1 a % Regeneration inhibition Maximum number of GUS foci / explant Portion of Leaf % Regenerating explants b.](https://reader035.fdocuments.net/reader035/viewer/2022070416/5697c0201a28abf838cd1fe1/html5/thumbnails/6.jpg)
a b
600 bp720 bp
1 2 3 4 5 6 +C -C M 1 2 3 4 5 6 +C -C M
c
Withaferin A
Withanone
Withanolide A
Fig. 6
1 2 3
![Page 7: Fig. 1. Kanamycin concentration in mg l -1 a % Regeneration inhibition Maximum number of GUS foci / explant Portion of Leaf % Regenerating explants b.](https://reader035.fdocuments.net/reader035/viewer/2022070416/5697c0201a28abf838cd1fe1/html5/thumbnails/7.jpg)
Supplementary Fig. 1 Schematic representation of chimeric vector pIG121Hm used in Withania coagulans transformation. LB left border; Pnos nopaline synthase promoter; nptII neomycin phosphotransferase gene; Tnos nopaline synthase terminator; P35S CaMV Cauliflower Mosaic Virus 35S promoter; gusA intron containing β-glucuronidase gene; hptII hygromycin phosphotransferase gene and RB right border of T-DNA.
EcoRIHindIII
Pnos Tnos TnosP35S hptIIIntron-gusA
LB
SacI
XbaIBamHI
SalISalI BamHI
RB
TnosnptII P35S