farm animal - AsianNet · 2014. 12. 19. · 2014 Product Catalogue. 310, rue Louis Pasteur 34790...

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With you at every step www.id-vet.com farm animal immunodiagnostics 2014 Product Catalogue

Transcript of farm animal - AsianNet · 2014. 12. 19. · 2014 Product Catalogue. 310, rue Louis Pasteur 34790...

Page 1: farm animal - AsianNet · 2014. 12. 19. · 2014 Product Catalogue. 310, rue Louis Pasteur 34790 Grabels - FRANCE Phone + 33 (0) 4 67 41 49 33 ... IDvet strives to be at the cutting

With you at every step

www.id-vet.com

farm animalimmunodiagnostics

2014 Product Catalogue

Page 2: farm animal - AsianNet · 2014. 12. 19. · 2014 Product Catalogue. 310, rue Louis Pasteur 34790 Grabels - FRANCE Phone + 33 (0) 4 67 41 49 33 ... IDvet strives to be at the cutting

310, rue Louis Pasteur34790 Grabels - FRANCE

Phone + 33 (0) 4 67 41 49 33Fax + 33 (0) 4 67 45 36 95

[email protected] www.id-vet.com

Ordering information

IDvet’s sales team and worldwide distribution network are at your service.

Please contact IDvet for technical support, or to locate the representative nearest you.

INNOVATIVE FARM ANIMAL DIAGNOSTICS

Located in Montpellier, France, IDvet develops, manufactures and sells diagnostic reagents for the detection of infectious diseases in farm animals.

With a focus on immunodiagnostics, and ELISA kits in particular, IDvet offers an extensive range of products for emerging diseases and diseases included in major prevention programs.

IDvet’s tests are used by private and public laboratories in surveillance programs and prevalence studies in over 70 countries.

HIGH-QUALITY PRODUCTS

IDvet ELISA kits, marketed under the tradename ID Screen®, contain all necessary reagents for sample testing, and are standardized to detect national and international reference sera.

The tests are robust and easy-to-use, and show innovation through simplified test protocols, ready-to-use reagents, and improved test performance.

Thanks to a highly-skilled scientific team, kit components are produced on-site to guarantee reagent quality.

Certified ISO 9001 : 2008, the company’s commitment to quality covers all activities, from Research & Development, to Production, Quality Control and Sales.

WITH YOU AT EVERY STEP

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SCIENTIFIC KNOW-HOW

IDvet strives to be at the cutting edge of veterinary diagnostics.

IDvet is the industrial partner in a number of European research programs, and collaborates closely with many international and national reference laboratories.

WITH YOU AT EVERY STEP

IDvet’s multi-lingual sales and technical team aims to provide the highest-level of customer service, offering efficient, professional technical support.

By adapting products to meet your needs, rapidly responding to your queries, providing free supplementary liquid reagents, and assisting you in kit use and result interpretation, IDvet is with you at every step.

WITH YOU AT EVERY STEP

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CoNTENTSRUMINANTS .............................................................................. 7Akabane ...............................................................................................................................8Besnoitiosis ..........................................................................................................................9BHV-4 .................................................................................................................................10Bluetongue .........................................................................................................................11Bovine Leukosis Virus ........................................................................................................14Bovine Viral Diarrhoea (BVD) / Border Disease (BD) / Mucosal Disease (MD) .................17Brucellosis ..........................................................................................................................20Chlamydophila abortus / Enzootic abortion ........................................................................24Interferon gamma ...............................................................................................................25Infectious Bovine Rhinotracheitis (IBR) ..............................................................................26Maedi Visna Virus / Caprine Arthritis Encephalitis Virus (CAEV) .......................................30Neospora caninum .............................................................................................................31Parainfluenza 3...................................................................................................................32Paratuberculosis / Johne’s Disease ...................................................................................33Peste des Petits Ruminants ...............................................................................................34Q Fever...............................................................................................................................37Rift Valley Fever .................................................................................................................38Schmallenberg virus ...........................................................................................................40Toxoplasmosis ....................................................................................................................43

EQUINE .................................................................................... 45Equine Infectious Anemia ...................................................................................................46Equine Viral Arteritis ...........................................................................................................49Influenza A ..........................................................................................................................50Strangles / Streptococcus equi ...........................................................................................52Trichinellosis .......................................................................................................................53West Nile Virus ...................................................................................................................54

AVIAN ....................................................................................... 57Infectious Bronchitis ...........................................................................................................58Infectious Bursal Disease / Gumboro Disease ...................................................................60Infectious Laryngotracheitis ................................................................................................62Influenza A ..........................................................................................................................63Avian Mycoplasmosis .........................................................................................................66Newcastle Disease .............................................................................................................70Salmonellosis .....................................................................................................................72West Nile Virus ...................................................................................................................73

SWINE ...................................................................................... 75Actinobacillus pleuropneumoniae .......................................................................................76Aujeszky’s Disease / Pseudorabies ....................................................................................78 African Swine Fever ...........................................................................................................80Brucellosis ..........................................................................................................................81Classical Swine Fever / Hog Cholera .................................................................................82Hepatitis E ..........................................................................................................................83Influenza A ..........................................................................................................................84Mycobacterium avium.........................................................................................................86Mycoplasma hyopneumoniae / Enzootic pneumoniae .......................................................87Swine Vesicular Disease ....................................................................................................89Toxoplasmosis ....................................................................................................................90Trichinellosis .......................................................................................................................91

COMPANION ANIMALS .......................................................... 93Canine Leishmaniasis ........................................................................................................94

ALL SPECIES .......................................................................... 95Complement Fixation Test ..................................................................................................96ID Soft™ Data Analysis Programme ..................................................................................97Positive and negative freeze-dried sera and milk ...............................................................98

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RumiNANTS

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Ruminants

Product code AKAC-4P AKAC-10P

Kit format 4 plates 10 plates

Reactions 384 960

Plate format 12 x 8-well strips

AkAbANE

Competitive ELISA for the detection of anti-G1 antibodies in ruminant serum and plasma.

ID Screen® Akabane Competition

Method Competitive ELISA

Species Ruminants

Specimens Serum and plasma

Coated antigen Purified virus

Conjugate Anti-G1-HRP conjugate (concentrated 10X)

Specifications

Ordering information

References

(1) Lunt, RA, White, JR, Newberry, KM, (CSIRO Livestock Industries, Geelong, Australia), Pourquier, P & Comtet, L (IDvet, Montpellier, France). Development of a commercial ELISA for the detection of serum antibody to Akabane virus. Poster presented at the 2007 WAVLD meeting, Melbourne, Australia.

(2) Comtet L. & Pourquier P. (IDvet, France). ID Screen® Schmallenberg virus Competition Multi-species: validation of a competitive ELISA for the detection of antibodies directed against the Schmallenberg virus nucleoprotein. Poster presented at the Epizone 7th Annual meeting, brussels, belgium, 2013.

High correlation with the virus neutralisation test (VNT)

Sensitive and specific, with clear separation of positive and negative results

Fast and convenient : ready-to-use components, with results in just over 2 hours.

Cost-effective for the analysis of large numbers of samples, notably in comparison with VNT.

No cross-reactions with SBV (2)

Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes colour upon addition of the sera

Associated Products

Akabane positive freeze-dried serumProduct Code MRI-AKA

Format 1 ml vial (freeze-dried)

Description Positive freeze-dried serum from an experimentally infected sheep, to be used as internal reference material for quality control.

Negative bovine freeze-dried serumProduct Code MRINEG-BOV

Format 0.5 ml vial (freeze-dried)

Description Negative freeze-dried bovine serum to be used as internal reference material for quality control.

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bESNoiTioSiS

Indirect ELISA kit for the detection of anti-Besnoitia besnoiti antibodies in bovine serum or plasma.

ID Screen® Besnoitia Indirect

Method Indirect ELISA

Species Cattle

Specimens Serum or plasma

Coated antigen Besnoitia besnoiti purified antigen

Conjugate Anti-ruminant IgG-HRP conjugate (concentrated 10X)

Specifications

Optimal specificity

High sensitivity

No sample pre-dilution required : sera are diluted directly in the ELISA plate

Easy-to-use, with results in just 90 minutes

References

Garcia-Lunar, P. et al. An Inter-Laboratory Comparative Study of Serological Tools Employed in the Diagnosis of Besnoitia besnoiti Infection in Bovines. Transboundary and Emerging Diseases, 2012. doi:10.1111/j.1865-1682.2012.01318.x

Ordering information

Product code BSNTS-2P

Kit format 2 plates

Reactions 192

Plate format 12 x 8-well strips

Associated Products

Besnoitia besnoiti positive freeze-dried serumProduct Code MRI-BSNT

Format 1 ml vial (freeze-dried)

Description Positive freeze-dried bovine serum to be used as internal reference material for quality control.

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Ruminants

Product code BHV4S-2P

Kit format 2 plates

Reactions 192

Plate format 12 x 8-well strips

bHV-4

Indirect ELISA kit for the detection of antibodies against Bovine Herpes Virus type 4 (BHV-4) in bovine serum or plasma.

ID Screen® BHV-4 Indirect

Method Indirect ELISA

Species Cattle

Specimens Serum or plasma

Coated antigen BHV-4 purified antigen

Conjugate Anti-ruminant IgG-HRP conjugate (concentrated 10X)

Specifications

Ordering information

References

Carpinschi, V, Treilles, M. (Departmental veterinary lab (LVD 50), Saint-Lo, France), Leboeuf, C. (Groupement de Défense Sanitaire, Saint-Lo, France), and Pourquier, P. (IDvet, France). Comparison of ELISA and IIF methods for the detection of BHV-4 antibodies in Bovine Serum. Poster presented at the WAVLD meeting, Madrid, 2009.

Accurate and reliable : clearly separates positive and negative results, with high sensitivity and specificity

Fast, easy-to-use, and robust, with results in 90 minutes

Associated Products

BHV-4 monoclonal antibodyProduct Code MAB-BHV4

Format 1 ml of purified monoclonal antibody, 1mg/ml.

Description Monoclonal antibody against Bovine Herpes Virus type 4 (BHV-4) for indirect immunofluorescence.

BHV-4 positive freeze-dried serumProduct Code MRI-BHV4

Format 0.5 ml vial (freeze-dried)

Description Positive freeze-dried bovine serum to be used as internal reference material for quality control.

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bLuEToNGuE

Competitive ELISA kit for the detection of anti-VP7 antibodies in serum or plasma from multiple species.

ID Screen® Bluetongue Competition

Method Competitive ELISA

Species Multiple species, including sheep, goats, cattle, buffalo, deer, and others

Specimens Serum or plasma

Coated antigen Recombinant VP7

Conjugate Anti-VP7-HRP conjugate (concentrated 10X)

Specifications

Proven specificity and sensitivity and widespread use in recent outbreaks

Detection of antibodies against all BTV serotypes, at least 5-8 days after natural infection, thanks to the use of a monoclonal antibody against the highly conserved VP7 protein

Easy-to-use : only one wash step, with results in 90 minutes

References

Vandenbussche, F., et al., Evaluation of antibody-ELISA and real-time RT-PCR for the diagnosis and profiling of bluetongue virus serotype 8 during the epidemic in Belgium in 2006., Vet. Microbiol. (2007), doi:10.1016 / j.vetmic.2007.10.029.

Ordering information

Product code BTC-4P BTC-10P

Kit format 4 plates 10 plates

Reactions 384 960

Plate format 12 x 8-well strips

Associated Products

Bluetongue positive freeze-dried serumProduct Code MRI-BTC

Format 1 ml vial (freeze-dried)

Description Positive freeze-dried bovine serum to be used as internal reference material for quality control.

Negative bovine freeze-dried serumProduct Code MRINEG-BOV

Format 0.5 ml vial (freeze-dried)

Description Negative freeze-dried bovine serum to be used as internal reference material for quality control.

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Ruminants

bLuEToNGuE

Double antigen ELISA for the early detection of anti-VP7 antibodies in serum or plasma from ruminants and other species. Efficiently detects both IgG and IgM antibodies against all BTV serotypes.

ID Screen® Bluetongue Early Detection*

Method Double antigen ELISA

Species Multiple species, including sheep, goat, cattle, buffalo, deer and others

Specimens Serum or plasma

Coated antigen Recombinant VP7

Conjugate Recombinant VP7 antigen-HRP conjugate (concentrated 10X)

Specifications

Early identifcation of infected and vaccinated animals thanks to the efficient detection of IgM antibodies and low antibody titres (IgG or IgM). Antibodies are detected 5-8 days after natural infection, and at least 7 days post- vaccination

Accurate and reliable : high specificity and sensitivity for all BTV serotypes

Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes colour upon addition of the sera

Ordering information

Product code BTED-4P

Kit format 4 plates

Reactions 384

Plate format 12 x 8-well strips

Associated Products

Bluetongue positive freeze-dried serumProduct Code MRI-BTC

Format 1 ml vial (freeze-dried)

Description Positive freeze-dried bovine serum to be used as internal reference material for quality control.

References

Oura, CAL. et al. Colostral antibody protection and interference with immunity in lambs born from sheep vaccinated with an inactivated Bluetongue serotype 8 vaccine. Vaccine (2010), doi: 10.1016/j.vaccine. 2010.01.028.

* Not available in Spain

Negative bovine freeze-dried serumProduct Code MRINEG-BOV

Format 0.5 ml vial (freeze-dried)

Description Negative freeze-dried bovine serum to be used as internal reference material for quality control.

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Product code BTSMILK-4P

Kit format 4 plates

Reactions 384

Plate format 12 x 8-well strips

bLuEToNGuE

Indirect ELISA for the detection of anti-VP7 antibodies in individual or bulk milk samples.

ID Screen® Bluetongue Milk Indirect

Method Indirect ELISA

Species Sheep, goat, cattle or buffalo

Specimens Individual or bulk milk samples.

Coated antigen Recombinant VP7

Conjugate Anti-ruminant IgG-HRP conjugate (concentrated 10X)

Specifications

Ordering information

References

Kramps, J.A, et al., Validation of a commercial ELISA for the detection of bluetongue virus (BTV)-specific antibodies in individual milk samples of Dutch dairy cows, Vet. Microbiol. (2008), doi: 10.1016/j.vetmic.2008.01.004.

The only commercial ELISA for the detection of BTV antibodies in milk

Cost-effective : the use of milk samples makes sample collection easier and more economical

Accurate, robust and reliable : high specificity and sensitivity, and excellent correlation with serum testing

Associated Products

Bluetongue positive freeze-dried milkProduct Code MRI-BTMILK

Format 1 ml vial (freeze-dried)

Description Freeze-dried positive bovine milk to be used as internal reference material for quality control.

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Ruminants

Product code BLVS-4P BLVS-10P

Kit format 4 plates 10 plates

Reactions 384 960

Plate format 12 x 8-well strips

boViNE LEukoSiS ViRuS

Indirect ELISA for the detection of anti-BLV antibodies in bovine or buffalo sera and plasma (individual and pools of ten samples).

ID Screen® BLV Indirect

Method Indirect ELISA

Species Cattle or buffalo

Specimens Serum and plasma (individual or pools of up to 10 samples)

Coated antigen Purified virus

Conjugate Anti-ruminant IgG-HRP Conjugate (concentrated 10x)

Specifications

Ordering information

References

(1) OIE Terrestrial Manual 2012; 2.4.11; Enzootic Bovine Leukosis.

(2) European directive CEE 64/432

Excellent specificity and sensitivity

Calibrated to detect the E05 serum standard1

Straightforward, versatile test, with short or overnight incubations for individual or pooled serum and plasma.

Negative bovine freeze-dried serumProduct Code MRINEG-BOV

Format 0.5 ml vial (freeze-dried)

Description Negative freeze-dried bovine serum to be used as internal reference material for quality control.

Associated Products

BLV positive freeze-dried serumProduct Code MRI-BLV

Format 1 ml vial (freeze-dried)

Description Positive freeze-dried bovine serum to be used as internal reference material for quality control

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Product code BLVC-4P BLVC-10P

Kit format 4 plates 10 plates

Reactions 384 960

Plate format 12 x 8-well strips

boViNE LEukoSiS ViRuS

Competitive ELISA kit for the detection of antibodies against the gp51 envelope protein of the Enzootic Bovine Leukosis Virus.

ID Screen® BLV Competition

Method Competitive ELISA

Species Cattle or buffalo

Specimens Serum or plasma (individual or pools of up to 10 samples)

Coated antigen Purified virus

Conjugate Anti-gp51-HRP Conjugate (concentrated 10x)

Specifications

Ordering information

References

(1) OIE Terrestrial Manual 2012; 2.4.11; Enzootic Bovine Leukosis.

(2) European directive CEE 64/432

Excellent specificity and sensitivity

Calibrated to detect the E05 serum standard1

Straightforward, versatile test, with short or overnight incubations for individual or pooled serum or plasma.

Negative bovine freeze-dried serumProduct Code MRINEG-BOV

Format 0.5 ml vial (freeze-dried)

Description Negative freeze-dried bovine serum to be used as internal reference material for quality control.

Associated Products

BLV positive freeze-dried serumProduct Code MRI-BLV

Format 1 ml vial (freeze-dried)

Description Positive freeze-dried bovine serum to be used as internal reference material for quality control

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Ruminants

Product code BLV-AGID BLV-AGID-NOGEL

Kit format Includes agar gel Agar gel not included

Reactions 240 240

boViNE LEukoSiS ViRuS

Agar gel immunodiffusion (AGID) test for the detection of BLV anti-gP51 antibodies in bovine serum.

IDvet BLV AGID

Method AGID

Species Bovine

Specimens Serum

Specifications

Ordering information

References

(1) OIE Terrestrial Manual 2012; 2.4.11; Enzootic Bovine Leukosis.

(2) European directive CEE 64/432

Bright, distinct precipitation lines facilitate test interpretation

Standardised against the European Standard E05

Associated Products

BLV AGID CUTTERProduct Code BLVCUTTER

Format As per the European Commission Decision of 15 December 2009 amending Annex D:• Diameter of central well: 4 mm• Diameter of peripheral wells: 6 mm• Distance between central and peripheral wells: 3 mm

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References

(1) OIE Terrestrial Manual 2008; 2.4.8; Bovine Viral Diarrhoea

boViNE ViRAL DiARRHoEA (bVD) / muCoSAL DiSEASE (mD)/ boRDER DiSEASE (bD)

Sandwich ELISA for the detection of the p80-125 protein of Bovine Viral Diarrhoea, Musocal Disease and Border Disease, in leukocyte fractions or ear notch samples from ruminants and all susceptible species.

ID Screen® BVD p80 Antigen

Method Sandwich ELISA

Species Bovine, ovine, caprine, and all susceptible species

Specimens Leukocyte fractions, ear notch samples

Coated antibody Anti-p80/125 monoclonal antibody

Conjugate Anti-p80/125-HRP conjugate (concentrated 10X)

Specifications

Ordering information

Product code BVDAG-4P BVDAG-10P

Kit format 4 plates 10 plates

Reactions 384 960

Plate format 12 x 8-well strip

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Ruminants

References

(1) OIE Terrestrial Manual 2008; 2.4.8; Bovine Viral Diarrhoea

boViNE ViRAL DiARRHoEA (bVD) / muCoSAL DiSEASE (mD)/ boRDER DiSEASE (bD)

One-stepTM ELISA for the detection of Bovine Viral Diarrhoea / Mucosal Disease / Border Disease anti-p80-125 (anti-NSP2-3) antibodies in serum and plasma from cattle, sheep, goats and other susceptible species.

ID Screen® BVD p80 Antibody One-Step™

Method One-StepTM ELISA

Species Cattle, sheep, goats and other susceptible species.

Specimens Serum or plasma

Coated antibody Anti-p80/125 Mab

Conjugate p80/125 antigen-HRP conjugate (concentrated 10X)

Specifications

Fast and easy-to-use : only one incubation step (co-incubation of serum and conjugate), with results in under 90 minutes

Accurate and reliable : high specificity and sensitivity

Ordering information

Product code BVDOS-4P

Kit format 4 plates

Reactions 384

Plate format 12 x 8-well strips

Associated Products

BVD/MD/BD positive freeze-dried serumProduct Code MRI-BVD

Format 1 ml vial (freeze-dried)

Description Antibody-positive freeze-dried bovine serum to be used as internal reference material for quality control.

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References

(1) OIE Terrestrial Manual 2008; 2.4.8; Bovine Viral Diarrhoea

boViNE ViRAL DiARRHoEA (bVD) / muCoSAL DiSEASE (mD)/ boRDER DiSEASE (bD)

Competitive ELISA for the detection of Bovine Viral Diarrhoea / Mucosal Disease / Border Disease anti-p80-125 (anti-NSP2-3) antibodies in serum, plasma and milk from cattle, sheep, goats and other susceptible species.

ID Screen® BVD p80 Antibody Competition

Fast and easy-to-use: ready-to-use conjugate and results in 90 minutes

Flexible : short and overnight protocols for serum, plasma or milk

Applicable to multiple species, including cattle, sheep, and goats

Accurate and reliable: high specificity and sensitivity

Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes colour upon addition of the sera

Method Competitive ELISA

Species Cattle, sheep, goats and other susceptible species.

Specimens Individual serum and plasma samplesMilk (individual samples or pools of up to 50)

Incubations Short and overnight incubations for serum, plasma and milk

Coated antigen p80/125 purified antigen

Conjugate Ready-to-use anti-p80/125-HRP monoclonal antibody

Specifications

Ordering information

Product code BVDC-4P BVDC-10P

Kit format 4 plates 10 plates

Reactions 384 960

Plate format 12 x 8-well strip

Associated Products

BVD/MD/BD positive freeze-dried serumProduct Code MRI-BVD

Format 1 ml vial (freeze-dried)

Description Antibody-positive freeze-dried bovine serum to be used as internal reference material for quality control.

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Ruminants

Product code AG-BRU

Kit format 100 ml

Reactions ~ 40000 reactions

bRuCELLoSiS

Brucella abortus antigen for the Complement Fixation Test.

Brucellosis Antigen for the Complement Fixation Test

Ordering information

References

(1) OIE Terrestrial Manual 2009; 2.4.3; Bovine brucellosis.

Suspension of Brucella abortus biovar 1 Weybridge strain No 99, inactivated by heat and phenol.

Calibrated to give 50% haemolysis with the second international standard serum (OIESS) diluted1/200

Associated Products

Brucellosis positive freeze-dried serumProduct Code MRI-BRU

Format 1 ml vial (freeze-dried)

Description Freeze-dried highly positive bovine serum to be used as internal reference material for quality control.

Brucellosis negative freeze-dried serumProduct Code MRINEG-BRU

Format 1 ml vial (freeze-dried)

Description Freeze-dried negative bovine serum to be used as internal reference material for quality control.

Guinea pig complementProduct Code CPLT-2x5ML

Format 2 x 5 ml vials

Description Freeze-dried guinea pig complement to be used in any complement fixation test (CFT).

Veronal bufferProduct Code VB-4L

Format 4 vials (powder form). Reconstitute each vial with 1L of distilled water

Description The Veronal Calcium Magnesium buffer is used to dilute sera and reagents for the complement fixation test.

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References

(1) OIE Terrestrial Manual 2009; 2.4.3; Bovine brucellosis.

(2) Annex C of the European directive CEE 64/432

bRuCELLoSiS

Rapid slide agglutination antigen for the detection of anti-Brucella abortus, melitensis, or suis antibodies in bovine, ovine, caprine, and porcine species.

Rose Bengal (Rapid slide agglutination antigen)

Suspension of Brucella abortus biovar 1 Weybridge strain No 99, inactivated by heat and phenol and coloured with rose bengal stain in an acidified buffer.

Calibrated on the OIEISS International Brucella standard serum and the national secondary serum to be positive against a 1/45 dilution and negative against a 1/55 dilution, as per the European directive CEE 64/432 and OIE requirements.

Ordering information

Product code RSA-RB

Kit format 10 ml

Reactions 330 reactions (30 µl per test)

Associated Products

Brucellosis positive freeze-dried serumProduct Code MRI-BRU

Format 1 ml vial (freeze-dried)

Description Freeze-dried highly positive bovine serum to be used as internal reference material for quality control.Positive when tested with Rose Bengal.

Brucellosis negative freeze-dried serumProduct Code MRINEG-BRU

Format 1 ml vial (freeze-dried)

Description Freeze-dried negative bovine serum to be used as internal reference material for quality control.Negative when tested with Rose Bengal.

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Ruminants

Product code BRUS-MS-4P BRUS-MS-10P

Kit format 4 plates 10 plates

Reactions 384 960

Plate format 12 x 8-well strips

bRuCELLoSiS

Indirect ELISA for the detection of antibodies against Brucella abortus, melitensis or suis in bovine, ovine, caprine and porcine serum and plasma (individual samples or pools of up to 10).

ID Screen® Brucellosis Serum Indirect Multi-species

Method Indirect ELISA

Species Bovine, ovine, caprine and porcine. Please contact IDvet for use in other species.

Specimens Individual serum or plasma samples, or pools of up to 10 samples

Coated antigen Purified Brucella LPS

Conjugate Anti-multi-species-IgG-HRP conjugate (concentrated 10X)

Specifications

Ordering information

References

(1) OIE Terrestrial Manual 2009; 2.4.3; Bovine brucellosis.

(2) Annex C of the European directive CEE 64/432

Calibrated according to OIE specifications and Annex C of the European directive CEE 64/432 to correctly detect the OIEELISASPISS standard serum.

Straightforward, versatile test, with short and overnight incubations for individual serum or plasma samples, or pools of up to 10 samples.

Multi-species test for ruminants and swine

Associated Products

Brucellosis positive freeze-dried serumProduct Code MRI-BRU

Format 1 ml vial (freeze-dried)

Description Freeze-dried highly positive bovine serum to be used as internal reference material for quality control.

Brucellosis negative freeze-dried serumProduct Code MRINEG-BRU

Format 1 ml vial (freeze-dried)

Description Freeze-dried negative bovine serum to be used as internal reference material for quality control.

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References

(1) OIE Terrestrial Manual 2009; 2.4.3; Bovine brucellosis.

(2) Annex C European directive CEE 64/432

bRuCELLoSiS

Indirect ELISA for the detection of antibodies against Brucella abortus or melitensis in bovine, ovine and caprine milk. The test may be used on individual or bulk milks*.The assessment of the diagnostic performance of this ELISA kit on field samples has shown that positive bovine milk samples may be detected in pools of up to 250 samples.

* Note: The analysis of milk from small ruminants and the analysis of individual milks are not official EU tests.

ID Screen® Brucellosis Milk Indirect

Calibrated according to the Annex C European directive CEE 64/432 and OIE requirements

Straightforward test for individual or pooled milks.

Ordering information

Product code BRUMILK-4P

Kit format 4 plates

Reactions 384

Plate format 12 x 8-well strips

Method Indirect ELISA

Species Ruminants

Specimens Bovine (cattle and buffalo), ovine and caprine milk (individual or up to 250 pooled samples).

Coated antigen Purified Brucella LPS

Conjugate Anti-ruminant IgG-HRP conjugate (concentrated 10X)

Specifications

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Ruminants

References

(1) Horigan, MW et al. (Veterinary Laboratories Agency, UK). Poster : Chlamydophila abortus – An Evaluation of Three Commercial ELISAs. Presented at the WAVLD Conference in madrid, Spain, 2009.

(2) Marques, PX et al. (University College, Dublin, Ireland). Poster: A comparison of three serological methods for the identification of pregnant ewes infected with Chlamydophila abortus. 2008.

(3) Pourquier, P. (IDvet, France), Rodalakis, A and Mohamad, KY (INRA, Nouzilly, France). Preliminary validation of a new commercial ELISA kit for the detection of antibodies directed against C. abortus. Presented at the WAVLD Conference, 2007.

CHLAmyDoPHiLA AboRTuS / ENzooTiC AboRTioN

This indirect multi-species ELISA detects anti-MOMP (Major Outer Membrane Protein) antibodies in ruminant serum and plasma. Please contact IDvet for use on other species.

ID Screen® Chlamydophila abortus Indirect Multi-species

Method Indirect ELISA

Species Ruminants and other species

Specimens Serum and plasma

Coated antigen MOMP peptide

Conjugate Anti-multi-species IgG–HRP conjugate (concentrated 10X)

Specifications

High sensitivity and superior specificity with respect to C. pecorum thanks to the use of peptide antigens

Applicable to multiple species, including sheep, goats and cattle. Please consult IDvet for use with other species

Ordering information

Product code CHLMS-MS-2P

Kit format 2 plates

Reactions 192

Plate format 12 x 8-well strips

Associated Products

Chlamydophila abortus positive freeze-dried serum

Product Code MRI-CHLM

Format 1 ml vial (freeze-dried)

Description Positive freeze-dried ovine serum to be used as internal reference material for quality control.

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Product code IFNG-2P IFNG-4P IFNG-10P

Kit format 2 plates 4 plates 10 plates

Reactions 192 384 960

Plate format 12 x 8-well strips

iNTERFERoN GAmmA

Sandwich ELISA for the detection of ovine, bovine and caprine interferon gamma (IFN-g ) in activated plasma or in culture supernatant.

ID Screen® Ruminant IFN-g

Method Sandwich ELISA

Species Bovine, ovine and caprine

Specimens Activated plasma or culture supernatant

Coated antibody Anti-ruminant IFN-g monoclonal antibody

Conjugate Anti-ruminant IFN-g HRP Mab conjugate

Specifications

Ordering information

References

Comtet, L., Pourquier, P. (IDvet). ID Screen® Interferon gamma ELISA: improvement of analytical sensitivity and introduction of a standard reference control to improve result interpretation. Poster presented at the 2012 International Colloquium on Paratuberculosis, Sydney, Australia.

Standardized results thanks to a freeze-dried positive reference control used to calculate S/P ratios for each sample.

Flexible: the same kit may be used with both native antigens (standard protocol) and recombinant / peptide antigens (alternative protocol)

Practical: the standard protocol uses only 10ul per sample, meaning that less sample volume is required for activation

Associated Products

Interferon gamma positive activated freeze-dried plasmasSpecies Bovine Ovine Caprine Buffalo

Product Code MRI-IFNG-B MRI-IFNG-O MRI-IFNG-C MRI-IFNG-BF

Format 0.5 ml vial (freeze-dried)

Reagents for in vitro cell activationProduct Code PPDPACK

Format Reagents for 5000 reactions

Description In vitro cell activation pack for 5000 reactions containing avian and bovine tuberculin PPD and mitogen (pokeweed). Mitogen is used as a positive activation control

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Ruminants

Product code IBRS-4P IBRS-10P

Kit format 4 plates 10 plates

Reactions 384 960

Plate format 12 x 8-well strips

iNFECTiouS boViNE RHiNoTRACHEiTiS (ibR)

Indirect ELISA for the detection of anti-BHV-1 (Bovine Herpesvirus type 1) antibodies in bovine serum and plasma.

ID Screen® IBR Indirect

Method Indirect ELISA

Species Cattle and buffalo

Specimens Serum and plasma

Coated antigen BHV-1 purified lysat

Conjugate Anti-ruminant IgG-HRP conjugate (concentrated 10X)

Specifications

Ordering information

References

(1) OIE Terrestrial Manual 2010; 2.4.13; Infectious bovine rhinotracheitis/infectious pustular vulvovaginitis.

Calibrated to detect the EU2 (weak-positive) standard serum 1

Straightforward and rapid, with short and overnight incubations and results in 90 minutes.

Associated Products

IBR positive freeze-dried serumProduct Code MRI-IBR

Format 1 ml vial (freeze-dried)

Description Freeze-dried positive bovine serum to be used as internal reference material for quality control.

Negative bovine freeze-dried serumProduct Code MRINEG-BOV

Format 0.5 ml vial (freeze-dried)

Description Negative freeze-dried bovine serum to be used as internal reference material for quality control.

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References

(1) OIE Terrestrial Manual 2010; 2.4.13; Infectious bovine rhinotracheitis/infectious pustular vulvovaginitis.

iNFECTiouS boViNE RHiNoTRACHEiTiS (ibR)

Indirect ELISA for the detection of anti-BHV-1 (Bovine Herpesvirus type 1) antibodies in bovine bulk milk.

ID Screen® IBR Milk Indirect

Method Indirect ELISA

Species Cattle and buffalo

Specimens Bulk milk

Coated antigen BHV-1 purified lysat

Conjugate Anti-ruminant IgG – HRP (concentrated 10X)

Specifications

Cost-effective: the use of milk samples makes sample collection easier and more economical.

Accurate, robust and reliable : high specificity and sensitivity, and excellent correlation with serumtesting

Rapid and easy-to-use

Short and overnight protocols

Ordering information

Product code IBRMILK-4P IBRMILK-10P

Kit format 4 plates 10 plates

Reactions 384 960

Plate format 12 x 8-well strips

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Ruminants

References

(1) OIE Terrestrial Manual 2010; 2.4.13; Infectious bovine rhinotracheitis/infectious pustular vulvovaginitis.

iNFECTiouS boViNE RHiNoTRACHEiTiS (ibR)

Competitive ELISA for the detection of anti-gB antibodies in serum and plasma.

ID Screen® IBR gB Competition

Method Competitive ELISA

Species Cattle and buffalo

Specimens Serum and plasma

Incubations Short and overnight

Coated antigen BHV-1 purified lysat

Conjugate Ready-to-use anti-gB-HRP conjugate

Specifications

Calibrated to detect the EU2 (weak-positive) standard serum1.

Flexible test: short and overnight protocols.

Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes colour upon addition of the sera

The stop solution no longer contains H2SO4, meaning that it is less corrosive

Ordering information

Product code IBRGBC-4P IBRGBC-10P

Kit format 4 plates 10 plates

Reactions 384 960

Plate format 12 x 8-well strips

Associated Products

IBR positive freeze-dried serumProduct Code MRI-IBR

Format 1 ml vial (freeze-dried)

Description Freeze-dried positive bovine serum to be used as internal reference material for quality control.

Negative bovine freeze-dried serumProduct Code MRINEG-BOV

Format 0.5 ml vial (freeze-dried)

Description Negative freeze-dried bovine serum to be used as internal reference material for quality control.

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References

(1) OIE Terrestrial Manual 2010; 2.4.13; Infectious bovine rhinotracheitis/infectious pustular vulvovaginitis.

iNFECTiouS boViNE RHiNoTRACHEiTiS (ibR)

Competitive ELISA for the detection of anti-BHV-1 gE antibodies in bovine serum, plasma and milk (individual, bulk or condensed milk samples).Detects naturally-infected animals in a vaccinated population provided that animals are vaccinated with BHV-1 gE-deleted vaccines.

ID Screen® IBR gE Competition

Method Competitive ELISA

Species Bovine

Specimens Serum, plasma and milk (individual, bulk or condensed milk samples)

Coated antigen BHV-1 purified lysat

Conjugate Anti-gE-HRP conjugate (concentrated 10X)

Specifications

May be used as a DIVA test (Differentiation of Infected and Vaccinated Animals)

Calibrated to detect the EU2 (weak-positive) standard serum1.

Ordering information

Product code IBRGEC-4P IBRGEC-10P

Kit format 4 plates 10 plates

Reactions 384 960

Plate format 12 x 8-well strips

Associated Products

IBR positive freeze-dried serumProduct Code MRI-IBR

Format 1 ml vial (freeze-dried)

Description Freeze-dried positive bovine serum to be used as internal reference material for quality control.

Negative bovine freeze-dried serumProduct Code MRINEG-BOV

Format 0.5 ml vial (freeze-dried)

Description Negative freeze-dried bovine serum to be used as internal reference material for quality control.

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Ruminants

References

(1) Comtet, L. (IDvet), Feliziani, F. (CEREL – IZS Umbria e Marche, Italy), Lesceu, S. (IDvet). Validation of the ID Screen® Maedi Visna Indirect ELISA: specificity on BTV-8 vaccinated sheep and detection of seroconversion. Poster presented at the 2010 EAVLD meeting, Lelystad, Holland.

mAEDi ViSNA ViRuS / CAPRiNE ARTHRiTiS ENCEPHALiTiS ViRuS (CAEV)

Indirect ELISA kit for the detection of anti-Maedi Visna Virus (MVV) and anti-CAEV antibodies in ovine and caprine serum. The use of a mix of peptide antigens results in superior test performance (sensitivity, specificity, and differentiation between positive and negative results).

ID Screen® MVV / CAEV Indirect

Method Indirect ELISA

Species Sheep and goats

Specimens Serum or plasma

Coated antigen Mix of peptides from the GAG, TM and ENV genes.

Conjugate Anti-ruminant IgG-HRP conjugate (concentrated 10X)

Specifications

Reliable and accurate : clearly separates positive and negative results, with high sensitivity and specificity

No non-specific reactions due to BTV-vaccination, thanks to the use of peptide antigens. Other ELISAs based on recombinant or purified viral antigens have given non-specific reactions in BTV-vaccinated populations 1

Easy-to-use, with results in 90 minutes

Ordering information

Associated Products

MVV / CAEV positive freeze-dried serumProduct Code MRI-VISNA / CAEV

Format 0.5 ml vial (freeze-dried)

Description Freeze-dried positive ovine and caprine serum pool to be used as internal reference material for quality control.

Confirmation format

Product code VISNAB-4P

Kit format 4 plates (bi-well)

Reactions 192

Plate format 6 x 16-well strips

Screening format

Product code VISNAS-4P

Kit format 4 plates

Reactions 384

Plate format 12 x 8-well strips

* Each sample is tested in duplicate in a coated well (even columns) and in an adjacent non-coated well (odd columns). Results are cal-culated with respect to the corrected sample oD value (even well oD – odd well oD).

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Product code NCS-MS-2P NCS-MS-5P

Kit format 2 plates 5 plates

Reactions 192 480

Plate format 12 x 8-well strips

NEoSPoRA CANiNum

Indirect multi-species ELISA kit for the detection of anti-Neospora caninum antibodies in serum or milk.

ID Screen® Neospora caninum Indirect Multi-species

Method Indirect ELISA

Species Multiple species, including ruminants and dogs.

Specimens Serum, plasma or milk

Coated antigen Purified Neospora caninum extract

Conjugate Anti-multi-species IgG –HRP conjugate (concen-trated 10X)

Specifications

Ordering information

Cost-effective method for disease monitoring, with high specificity and sensitivity

Fast and convenient protocol with results in 60 minutes and protocols for both serum and milk samples

Applicable to multiple species, including ruminants and dogs.

Associated Products

N. caninum positive freeze-dried serumProduct Code MRI-NC

Format 0.5 ml vial (freeze-dried)

Description Freeze-dried positive bovine serum to be used as internal reference material for quality control

Negative bovine freeze-dried serumProduct Code MRINEG-BOV

Format 0.5 ml vial (freeze-dried)

Description Negative freeze-dried bovine serum to be used as internal reference material for quality control.

References

Alvarez-García, G., et al., Serological diagnosis of bovine neosporosis: A comparative study of commercially available ELISA tests. Vet. Parasitol. (2013), http://dx.doi.org/10.1016/j.vetpar.2013.07.033

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Ruminants

PARAiNFLuENzA 3

PI-3 monoclonal antibody

Product code MAB-PI3

Description Monoclonal antibody against Parainfluenza-3 virus for indirect immunofluorescence

Format 1 ml of purified monoclonal antibody, 1mg/ml

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PARATubERCuLoSiS / JoHNE’S DiSEASE

Indirect absorbed(3) ELISA for the detection of anti-Mycobacterium avium subsp. paratuberculosis (Map) antibodies in ruminant serum, plasma and milk.

ID Screen® Paratuberculosis Indirect

Method Indirect absorbed ELISA

Species Ovine, caprine, bovine (cattle or buffalo)

Specimens Serum, plasma or bovine milk

Coated antigen Purified MAP extract

Conjugate Anti-ruminant-HRP conjugate (concentrated 10X)

Incubations Short or overnight

Specifications

Ideal for herd certification and disease eradication programs thanks to high sensitivity, specificity and clear separation of positive and negative results

Automatable and rapid : results in 90 minutes.

Screening and confirmation formats available

Short or overnight incubations

Screening format

Product code PARAS-4P PARAS-10P

Kit format 4 plates 10 plates

Reactions 384 960

Plate format 12 x 8-well strips

Confirmation format*

Product code PARAB-4P

Kit format 4 plates (bi-well)

Reactions 192

Plate format 6 x 16-well strips

Associated Products

Mycobactine JProduct Code MYCO

Format 2 mg vial (freeze-dried)

Description Ferric Mycobactin J is an iron-chelated substance used as a growth factor for the isolation of mycobactin-dependent Mycobacterium.

Paratuberculosis positive freeze-dried serumProduct Code MRI-PARA

Format 1 ml vial (freeze-dried)

Description Freeze-dried positive bovine serum to be used as internal reference material for quality control.

Ordering information

References

(1) Köhler, Heike et al. Evaluation of five ELISA test kits for the measurement of antibodies against Mycobacterium avium subspecies paratuberculosis in bovine serum. Berl. Münch. Tierärztl. Wochenschr. 121, Heft 5/6, 203-210 (2008).

(2) Fry, M., Kruze, J., Collins, M., Evaluation of four commercial enzyme-linked immunosorbent assays for the diagnosis of bovine paratuberculosis in Chilean dairy herds. J Vet Diagn Invest 20:329-332 (2008).

(3) OIE recommendations: Manual of Recommended Diagnostic Techniques and Requirements volume III -Paratuberculosis 5B/009.

* Each sample is tested in a coated well (even columns) and in an adjacent non-coated well (odd columns). Results are calculated with respect to the corrected sample oD (even well oD – odd well oD).

Paratuberculosis negative freeze-dried serumProduct Code MRINEG-PARA

Format 2 ml vial (freeze-dried)

Description Freeze-dried negative bovine serum to be used as internal reference material for quality control.

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Product code PPRC-4P PPRC-10P

Kit format 4 plates 10 plates

Reactions 384 960

Plate format 12 x 8-well strips

PESTE DES PETiTS RumiNANTS

Competitive ELISA kit for the detection of anti-PPRV nucleoprotein (NP) antibodies in sheep and goat serum or plasma. Please contact IDvet for use on other species.

The only commercial ELISA approved by the FAO / OIE CIRAD laboratory, and based on the CIRAD biological components (antigen and conjugate).

This test does not contain any virus particles.

ID Screen® PPR Competition

Method Competitive ELISA

Species Multiple species, including sheep, goats and others

Specimens Serum or plasma

Coated antigen Recombinant PPRV nucleoprotein (NP)

Conjugate Anti-NP-HRP conjugate (concentrated 10X)

Specifications

Ordering information

References

Libeau, G., Préhaud, C., Lancelot, R., Colas, F., Guerre, L., Bishop, D.H., Diallo, A. Development of a competitive ELISA for detecting antibodies to the Peste des Petits Ruminants virus using a recombinant nucleoprotein. Res Vet Sci. 1995 Jan;58(1):50-5.

Convenient format: The only commercial ELISA to supply ready-to-use, coated plates, and all other components in liquid format. Plates are supplied in strips

Straightforward and rapid protocol : each sample is deposited only once (screening format), and results are obtained in 90 minutes

High specificity and sensitivity

Associated Products

PPR positive freeze-dried serumProduct Code MRI-PPR

Format 1 ml vial (freeze-dried)

Description Freeze-dried positive caprine serum to be used as internal reference material for quality control.

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Product code PPRAG-1P PPRAG-2P

Kit format 1 plate 2 plates

Reactions 96 192

Plate format 12 x 8-well strips

PESTE DES PETiTS RumiNANTS

Sandwich ELISA for the detection of Peste des Petits Ruminants virus in live animals or for post-mortem diagnosis.

Based on reagents developed at a FAO/OIE PPRV reference laboratory (CIRAD, Montpellier, France). IDvet contributed to the improvement of this test by optimizing test performance and component stability.

This test does not contain any virus particles.

ID Screen® PPR Antigen Capture

Method Sandwich ELISA

Species Ovine and caprine

Specimens Variety of sample types, including oral, nasal or rectal swabs as well as tears, tissue samples, gum debris or PBMC

Coated antibody Anti-PPRV NP antibody

Conjugate Anti-PPRV NP Mab–HRP conjugate (concentrated 10X)

Specifications

Ordering information

References

(1) O. Kwiatek, Y.H. Ali, I.K. Saeed, A.I. Khalafalla, O.I. Mohamed, A.A. Obeida, M.B. Abdelrahman, H.M. Osman, K.M. Taha, Z. Abbas, M. El Harrak, Y. Lhor, A. Diallo, R. Lancelot, E. Albina, G. Libeau. Asian lineage of peste des petits ruminants virus, Africa. Emerg. Infect. Dis., 17 (2011), pp. 1223–1231

(2) Recognizing peste des petits ruminants - A field manual, FAO, section: diagnosis of PPR. URL link: http://www.fao.org/docrep/003/x1703e/x1703e00.htm#Diagnosis of PPR

(3) Libeau, G., Diallo, A., Colas, F., Guerre, L., 1994. Rapid differential diagnosis of rinderpest and peste des petits ruminants using an immunocapture ELISA. Vet.Rec. 19, 300–304

(4) Abubakar, M., et al. Comparison of antigen detection methods of peste des petits ruminants virus in clinical samples of small ruminants. Bulgarian Journal of Veterinary Medicine (2011), 14, No 2, 103-108

(5) Couacy-Hymann, E., et al. The early detection of peste-des-petits-ruminants (PPR) virus antigens and nucleic acid from experimentally infected goats using RT-PCR and immunocapture ELISA techniques. Research in Veterinary Science, Volume 87, Issue 2, October 2009, 332335

(6) Muhammad M., et al. (2012). Serodiagnosis of Peste des Petits Ruminants Virus, Serological Diagnosis of Certain Human, Animal and Plant Diseases, Dr. Moslih Al-Moslih (Ed.), ISBN: 978-953-51-0370-7, InTech, Available from: http://www.intechopen.com/books/serologicaldiagnosis-of-certain-human-animal-and-plant-diseases/serodiagnosis-of-peste-des-petits-ruminants-virus

(7) Comtet, L., Pourquier, P. (IDvet, France), Kwiatek, O., Libeau, G. (CIRAD, France). Validation of the ID Screen® Peste des Petits Ruminants Antigen Capture ELISA. Poster presented at the Epizone 7th Annual meeting, brussels, belgium, 2013.

Equivalent performance to RT-PCR 7

Improved sensitivity for samples with weak or strong PPRV titres

Flexible test for use with a wide variety of matrices, including oral, nasal or rectal swabs as well as tears, tissue samples, gum debris or PbmC.

Convenient and easy-to-use: microplates are supplied coated in strips of 8 wells.

Straightforward and rapid protocol, with results in 90 minutes

Associated Products

Freeze-dried PPR AntigenProduct Code MRI-PPRAG

Format 1 ml vial (freeze-dried)

Description Freeze-dried inactivated PPR antigen to be used as internal reference material for quality control.

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Q FEVER

Indirect multi-species ELISA for the detection of anti-Coxiella burnetii antibodies in serum and plasma or in individual or bulk milk samples from multiple species.

ID Screen® Q Fever Indirect Multi-species

Method Indirect ELISA

Species Multiple species, including cattle, sheep, goatsand others

Specimens Serum, plasma or milk

Coated antigen Inactivated Coxiella burnetii antigen from a bovine isolate

Conjugate Anti-multi-species IgG-HRP conjugate (concentrated 10X)

Specifications

Applicable to multiple species, including cattle, sheep and goats. Please contact iDvet for use in other species.

Straightforward, easy-to-use, with results in 90 minutes

Ordering information

Product code FQS-MS-2P

Kit format 2 plates

Reactions 192

Plate format 12 x 8-well strips

Associated Products

Q Fever positive freeze-dried serumProduct Code MRI-FQ

Format 1 ml vial (freeze-dried)

Description Freeze-dried positive bovine serum to be used as internal reference material for quality control

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Ruminants

Product code RIFTC-4P RIFTC-10P

Kit format 4 plates 10 plates

Reactions 384 960

Plate format 12 x 8-well strips

RiFT VALLEy FEVER

Competitive ELISA kit for the detection of anti-Rift Valley Fever (RVFV) nucleoprotein antibodies in ruminant serum or plasma.

This test does not contain any virus particles.

ID Screen® Rift Valley Fever Competition Multi-species

Method Competitive ELISA

Species Multiple species, including ruminants, camels, horses, dogs and others

Specimens Serum or plasma

Coated antigen RVF Virus recombinant nucleoprotein (NP)

Conjugate Anti-NP-HRP conjugate (concentrated 10X)

Specifications

Ordering information

References

(1) Comtet, L. et Pourquier, P. (IDvet), Marié, J-L. & Davoust, B. (French Defence Medical Service, Working group of animal epidemiology, Marseille, France), Cêtre-Sossah, C. (CIRAD, Montpellier, France). Preliminary validation of the ID Screen® Rift Valley Fever Competition Multi-species ELISA. Poster presented at the 2010 EAVLD meeting, Lelystad, The Netherlands.

(2) Ahmed B. et al. Unexpected Rift Valley Fever Outbreak, Northern Mauritania. Emerging Infectious Diseases. Vol. 17, No. 10. October 2011.

(3) Kortekaas, J. et al. European ring trial to evaluate ELISAs for the diagnosis of infection with Rift Valley fever virus. Journal of Virological Methods. September 2012.

(4) Antonis, A.F.G. et al. Vertical Transmission of Rift Valley Fever Virus Without Detectable Maternal Viremia. Vector-borne and zoonotic diseases. Vol. 13, No. X. May 2013.

Excellent analytical sensitivity, sensitivity and specificity, as proven in recent outbreaks

Multi-species test : may be used on ruminants, horses, dogs and other species

Convenient and rapid format: ready-to-use, coated plates in strips, and all other components in liquid format. Results in less than 2 hours

Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes colour upon addition of the sera

Associated Products

Rift Valley Fever IgG-positive freeze-dried serumProduct Code MRI-RIFT

Format 1 ml vial (freeze-dried)

Description Freeze-dried positive bovine serum to be used as internal reference material for quality control

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RiFT VALLEy FEVER

IgM Antibody Capture (IMAC) ELISA for the detection of anti-nucleoprotein (NP) IgM antibodies in ruminant serum and plasma. The detection of anti-IgM antibodies indicates recent infection.

This test does not contain any virus particles.

ID Screen® Rift Valley Fever IgM Capture

Specifications

Accurate and reliable : excellent specificity and analytical and diagnostic sensitivity

Convenient format: Ready-to-use, coated plates in strips, and all other components in liquid format.

Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes colour upon addition of the sera

Product code RIFTIGM-4P RIFTIGM-10P

Kit format 4 plates (biwell format*)

10 plates (biwell format*)

Reactions 192 480

Plate format 6 x 16 well strips

Ordering information

Method IMAC (IgM Antibody Capture) ELISA

Species Ruminants

Specimens Serum or plasma

Coated antibody Anti-bovine-ovine-caprine IgM antibody

Antigen RVFV recombinant nucleoprotein (freeze-dried)

Conjugate Anti-NP-HRP conjugate (concentrated 10X)

Protocol* (1) Sample Incubation(2) Three washes(3) RVF nucleoprotein incubation(4) Three washes(5) Anti-NP-HRP conjugate incubation (6) Three washes(7) Substrate Incubation

* Each sample is tested in duplicate in an antigen-coated well (even columns) and in an adjacent non-coated well (odd columns). Results are calculated with respect to the corrected sample oD value (even well oD – odd well oD).

Associated Products

Rift Valley Fever IgM-positive freeze-dried serumProduct Code MRI-RIFTIGM

Format 0.5 ml vial (freeze-dried)

Description Freeze-dried anti-RVFV recombinant NP ovine serum to be used as internal reference material for quality control

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Ruminants

SCHmALLENbERG ViRuS

Indirect ELISA kit for the detection of antibodies directed against the Schmallenberg virus (SBV) nucleoprotein (NP) in serum and plasma from multiple species, including cattle, sheep and goats. Please contact IDvet for use on other species.

ID Screen® Schmallenberg virus Indirect Multi-species

Method Indirect ELISA

Species Ruminants and other species

Specimens Serum, plasma

Coated antigen Recombinant SBV nucleoprotein antigen

Conjugate Anti-multi-species IgG-HRP conjugate (concentrated 10X)

Specifications

Ordering information

The first commercial ELISA for SBV serology

High sensitivity and specificity in external validation studies

Approved by the ANSES (France) and FLI (Germany) laboratories

Automatable and therefore suited to high throughput testing

Screening and confirmation formats available

* Each sample is tested in duplicate in a coated well (even columns) and in an adjacent non-coated well (odd columns). Results are calculated with respect to the corrected sample oD value (even well oD – odd well oD).

Confirmation format*

Product code SBVB-4P SBVB-10P

Kit format 4 plates (bi well) 10 plates (bi well)

Reactions 192 480

Plate format 6 x 16 well strips

Screening format

Product code SBVS-2P SBVS-4P

Kit format 2 plates 4 plates

Reactions 192 384

Plate format 12 x 8-well strips

References

(1) Jack C., Anstaett O, Adams J, Noad R, Browlie J., Mertens P. Evidence of seroconversion to SBV in camelids. Veterinary Record 2012 170:603 doi: 10.1136/vr.e3939

(2) Kaba et al. Schmallenberg Virus Antibodies Detected in Poland. Transboundary and Emerging Diseases. Rapid Communication. 2012.

(3) Linden A, Desmecht D, Volpe R, Wirtgen M, Pirson J, Paternostre J, et al. Epizootic Spreads of Schmallenberg virus among wild cervids, Belgium, fall 2011. Emerg Infect Dis (Internet). 2012 Dec.

(4) Bréard E, Lara E, Comtet L, Viarouge C, Doceul V, et al. (2013) Validation of a Commercially Available Indirect Elisa Using a Nucleocapside Recombinant Protein for Detection of Schmallenberg Virus Antibodies. PLoS ONE 8(1): e53446. doi:10.1371/journal.pone.0053446

Schmallenberg virus positive freeze-dried serumProduct Code MRI-SBV

Format 0.5 ml vial (freeze-dried)

Description Freeze-dried positive bovine serum to be used as internal reference material for quality control.

Associated Products

Negative bovine freeze-dried serumProduct Code MRINEG-BOV

Format 0.5 ml vial (freeze-dried)

Description Negative freeze-dried bovine serum to be used as internal reference material for quality control.

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SCHmALLENbERG ViRuS

Competitive ELISA for the detection of antibodies directed against the Schmallenberg virus (SBV) nucleoprotein (NP) in serum or plasma from multiple-species .

ID Screen® Schmallenberg virus Competition Multi-species

Specifications

The only competitive ELISA for SBV serology

Applicable to multiple species, including cattle, sheep and goats

Improved sensitivity for sera with low VNT titres

Earlier detection of seroconversion

Improved specificity

Robust and easy-to-use: the conjugate is supplied ready-to-use, and results are obtained in 90 minutes

Product code SBVC-2P SBVC-4P

Kit format 2 plates 4plates

Reactions 192 384

Plate format 12 x 8 well strips

Ordering information

Method Competitive ELISA

Species Ruminants and other species

Specimens Serum and plasma

Coated antigen Recombinant SBV nucleoprotein antigen

Conjugate Ready-to-use anti-SBV nucleoprotein-HRP conjugate

Schmallenberg virus positive freeze-dried serumProduct Code MRI-SBV

Format 0.5 ml vial (freeze-dried)

Description Freeze-dried positive bovine serum to be used as internal reference material for quality control.

Associated Products

Negative bovine freeze-dried serumProduct Code MRINEG-BOV

Format 0.5 ml vial (freeze-dried)

Description Negative freeze-dried bovine serum to be used as internal reference material for quality control.

(1) Comtet L. & Pourquier P. (IDvet, France). ID Screen® Schmallenberg virus Competition Multi-species: validation of a competitive ELISA for the detection of antibodies directed against the Schmallenberg virus nucleoprotein. Poster presented at the Epizone 7th Annual meeting, brussels, belgium, 2013.

References

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Ruminants

SCHmALLENbERG ViRuS

Indirect ELISA kit for the detection of antibodies directed against the Schmallenberg virus (SBV) nucleoprotein (NP) in ruminant individual and bulk milks.

ID Screen® Schmallenberg virus Milk Indirect

Specifications

The only commercial ELISA for the detection of SBV NP antibodies in milk

Ideal for monitoring the spread of the virus in disease-free regions

Cost-effective: the use of milk samples makes sample collection easier and more economical.

Accurate, robust and reliable : high specificity and sensitivity, and excellent correlation with serumtesting

Product code SBVMILK-2P SBVMILK-4P

Kit format 2 plates 4plates

Reactions 192 384

Plate format 12 x 8 well strips

Ordering information

Method Indirect ELISA

Species Bovine, ovine and caprine

Specimens Individual or bulk milks

Coated antigen Recombinant SBV antigen

Conjugate Anti-multi-species IgG-HRP conjugate(concentrated 10X)

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Ordering information

References

Sheep (1) Proctor AF et al. Detection of antibodies to Toxoplasma gondii in serum from experimentally infected pregnant ewes. University College Dublin, 2008(2) Mangili, P.M. et al. Development and evaluation of the performance of an in-house ELISA to be used for the indirect diagnosis of Toxoplasmosis in sheep. Poster presented at the SIDILV meeting in Parma, Italy, 2009.

Swine(3) Hotea, I. et al. Seroprevalence of Toxoplasma gondii infection in pigs reared in intensive system from Timis country. Lucrari Stiintice Medicina veterinara Vol. XLIII(1), 2010, Timisoara.(4) Görlich, K. et al. Validation, optimisation and standardisation of an automated test system for monitoring and surveillance of Toxoplasmosa gondii in pigs. 2010. Poster presented at Nationales Zoonose Symposium, Berlin, 2009.(5) Gómez-Laguna, J. et al. Seroprevalence of zoonotic diseases in Iberian pigs. SUIS N° 74, Enero/Febrero 2011.

Deer(6) Gaffuri, A., et al. Toxoplasma gondii in wild boar and roe deer in Northern Italy: serosurvey and PCR-RFLP. 9th Ewda Conference, 2010.(7) Renzi, M. et al. Serological investigation on the spread of Toxoplasma gondii in roe deer (Capreolus capreolus) Emilia-Romagna. Poster presented at the 3rd National SIEF Congress, Torino, 2009.

Dogs and cats(8) Scarpulla, M. et al. Comparison of indirect immunofluorescence and ID Screen® Toxoplasmosis indirect ELISA for the detection of antibodies against Toxoplasma gondii in cat and dog sera. Poster presented at the WAVLD meeting, Madrid, 2009.

Wild & domestic animals(9) Roqueplo, C. et al. Toxoplasma gondii in wild and domestic animals from New Caledonia. Parasite, 2011, 18, 345-348.

ToxoPLASmoSiS

Indirect multi-species ELISA for the detection of anti-Toxoplasma gondii antibodies in serum, plasma or meat juice from multiple species, including ruminants, swine, and cats. Please contact IDvet for use with other species.

ID Screen® Toxoplasmosis Indirect Multi-species

Method Indirect ELISA

Species Ruminants, swine, dogs and cats.

Specimens Serum, plasma or meat juice

Coated antigen Toxoplasma gondii P30 antigen

Conjugate Anti-multi-species IgG-HRP conjugate (concentrated 10X)

Specifications

Easy-to-use, with results in 90 minutes

Versatile protocol for serum, plasma or meat juice, and applicable to multiple species, including ruminants, swine, dogs and cats

Product code TOXOS-MS-2P

Kit format 2 plates

Reactions 192

Plate format 12 x 8-well strips

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Birds: Bertran, K. et al. Pathogenesis and transmissibility of highly (H7N1) and low (H7N9) pathogenic avian influenza virus infection in red-legged partridge (Alectoris rufa). Veterinary Research 2011, 42:24.Dundon, W.G., Terregino, C., Tuttoilmondo V., Pizzuto, M., Busani L., Mancin, M., Cattoli, G., Capua I.. Preliminary validation of a commercial Avian Influenza N1 antibody competitive Elisa kit that can be used as part of a Diva strategy. Epizone 2007, Poland.Molia, S. et al. Avian influenza in backyard poultry of the Mopti region, Mali. Trop Anim Health Prod. 2009. Terregino, C. Evaluation of sensitivity and specificity of a commercial competitive avian influenza type A antibody ELISA kit, the OIE-FAO and National Reference Laboratory for Newcastle Disease and Avian Influenza, IZS delle Venezie, Legnaro (Padova), Italy.Horses: Kittelberger, R., McFadden, A.M.J., Hannah, M.J., Jenner, J., Bueno, R., Vait, J., Kirkland, P.D., Delbridge, G., Heine, H.G., Selleck, P.W., Pearce, T.W., Pigott, C.J., O’Keefe, J.S., Comparative evaluation of four competitive/blocking ELISAs for the detection of influenza A antibodies in horses, Veterinary Microbiology (2010), doi:10.1016/j.vetmic.2010.08.014.Swine :Busquets, N. et al. Experimental infection with H1N1 European swine influenza virus protects pigs from an infection with the 2009 pandemic H1N1 human influenza virus. Vet. Res (2010) 41:74. Lange, E. et al. Pathogenesis and transmission of the novel swine-origin influenza virus A/H1N1 after experimental infection of pigs. Journal of General Virology (2009), 90, 2119-2123.Dogs:7 De Benedictis, P. et al. A diagnostic algorithm for detection of antibodies to influenza A viruses in dogs in Italy (2006-2008). J. Vet Diagn Invest 22:914-920 (2010).

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EQuiNE

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EQUINE

Product code EIAED-1P EIAED-4P

Kit format 1 plate 4 plates

Reactions 96 384

Plate format 12 x 8-well strips

EQuiNE iNFECTiouS ANEmiA

Double antigen ELISA kit for the detection of antibodies directed against the p26 protein of the Equine Infectious Anemia Virus (EIAV) in horse serum and plasma.

ID Screen® Equine Infectious Anemia Double Antigen

Associated Products

Method Double antigen ELISA

Species Equine

Specimens Serum and plasma

Coated antigen p26 recombinant antigen

Conjugate p26 antigen-HRP conjugate (concentrated 10X)

Highly sensitive and specific

Calibrated to detect the OIE standard positive anti-EIAV serum

Fast and easy-to-use, with results in 90 minutes

EIA positive freeze-dried serumProduct Code MRI-EIA

Format 1 ml vial (freeze-dried)

Description Freeze-dried positive horse serum to be used as internal reference material for quality control.

Specifications

Ordering information

References

Pourquier, P. et al. Validation of the ID Screen® Equine Infectious Anemia Double Antigen ELISA. Poster presented at the 2010 EAVLD meeting, Lelystad, The Netherlands.

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Associated Products

Product code EIA-AGID EIA-AGID-NOGEL

Kit format Includes agar gel Agar gel not included

Reactions 200 200

EQuiNE iNFECTiouS ANEmiA

Agar gel immunodiffusion (AGID) test for the detection of EIA anti-p26 antibodies in equine serum.

IDvet EIA AGID

Method AGID (Coggins-test)

Species Equine

Specimens Serum

Antigen EIAV p26

Clear, bright lines for easy reading

OIE-prescribed protocol1

24-month shelf-life

May be purchased with or without agar gel (the iDvet agar gel allows for clearer precipitation lines)

Specifications

Ordering information

References

OIE Terrestrial Manual 2013; 2.5.6; Equine Infectious Anemia

EIA AGID CUTTERProduct Code EIACUTTER

Format As per OIE specifications: • A seven-well pattern is used with one centre well encircled by 6 wells• The wells are 2.4 mm apart and 5.3 mm in diameter

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EQUINE

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Product code EVAB-4P

Kit format 4 plates (Biwell format)*

Reactions 192

Plate format 6 x 16-well strips

EQuiNE ViRAL ARTERiTiS

Indirect confirmation ELISA kit for the detection of anti-EVA antibodies in horse serum and plasma.

ID Screen® Equine Viral Arteritis Indirect*

Associated Products

Method Indirect ELISA

Species Equine

Specimens Serum and plasma

Coated antigen Specific EVA peptide

Conjugate Anti-horse IgG-HRP conjugate (concentrated 10X)

Compatible with cytotoxic sera1 which cannot be tested with the virus neutralization test (VNT)

Easy-to-use, with results in just over 2 hours

EVA positive freeze-dried serumProduct Code MRI-EVA

Format 0.5 ml vial (freeze-dried)

Description Freeze-dried positive horse serum to be used as internal reference material for quality control.

Specifications

Ordering information

References

(1) Legrand, L., Pitel, P.H., Fotier, G., Pronost, S. Testing for antibodies to equine arteritis virus. The Veterinary Record, April 4th, 2009.

* Each sample is tested in duplicate in a coated well (even columns) and in an adjacent non-coated well (odd columns). Results are calculated with respect to the corrected sample oD value (even well oD – odd well oD).

* Not available in France

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EQUINE

Product code FLUACA-2P FLUACA-4P FLUACA-10P

Kit format 2 plates 4 plates 10 plates

Reactions 192 384 960

Plate format 12 x 8-well strips

iNFLuENzA A

Competitive ELISA kit for the detection of anti-nucleoprotein (NP) antibodies. Detects antibodies against all Influenza A subtypes and antigenic variants in multiple species thanks to the use of a monoclonal antibody against a highly conserved epitope of the Influenza A virus nucleoprotein.

ID Screen® Influenza A Antibody Competition Multi-species

Method Competitive ELISA

Species Multiple species, including horses, birds, swine and others.

Specimens Serum and plasma

Coated antigen Influenza A nucleoprotein (NP)

Conjugate Anti-NP-HRP conjugate (concentrated 10X)

Highly sensitive and specific

Convenient multi-species test applicable to birds, horses, swine and others

Easy-to-use, with results in under 2 hours

Specifications

Ordering information

References

Horses: (1) Kittelberger, R., McFadden, A.M.J., Hannah, M.J., Jenner, J., Bueno, R., Vait, J., Kirkland, P.D., Delbridge, G., Heine, H.G., Selleck, P.W., Pearce, T.W., Pigott, C.J., O’Keefe, J.S., Comparative evaluation of four competitive/blocking ELISAs for the detection of influenza A antibodies in horses, Veterinary Microbiology (2010), doi:10.1016/j.vetmic.2010.08.014.Birds: (2) Bertran, K. et al. Pathogenesis and transmissibility of highly (H7N1) and low (H7N9) pathogenic avian influenza virus infection in red-legged partridge (Alectoris rufa). Veterinary Research 2011, 42:24.(3) Dundon, W.G., Terregino, C., Tuttoilmondo V., Pizzuto, M., Busani L., Mancin, M., Cattoli, G., Capua I.. Preliminary validation of a commercial Avian Influenza N1 antibody competitive Elisa kit that can be used as part of a Diva strategy. Epizone 2007, Poland.(4) Molia, S. et al. Avian influenza in backyard poultry of the Mopti region, Mali. Trop Anim Health Prod. 2009. (5) Terregino, C. Evaluation of sensitivity and specificity of a commercial competitive avian influenza type A antibody ELISA kit, the OIE-FAO and National Reference Laboratory for Newcastle Disease and Avian Influenza, IZS delle Venezie, Legnaro (Padova), Italy.Swine :(6) Busquets, N. et al. Experimental infection with H1N1 European swine influenza virus protects pigs from an infection with the 2009 pandemic H1N1 human influenza virus. Vet. Res (2010) 41:74. (7) Lange, E. et al. Pathogenesis and transmission of the novel swine-origin influenza virus A/H1N1 after experimental infection of pigs. Journal of General Virology (2009), 90, 2119-2123.Dogs:(8) De Benedictis, P. et al. A diagnostic algorithm for detection of antibodies to influenza A viruses in dogs in Italy (2006-2008). J. Vet Diagn Invest 22:914-920 (2010).

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Product code INFLAG-2P

Kit format 2 plates

Reactions 192

Plate format 96 individual wells

iNFLuENzA A

Capture ELISA for the detection of the Influenza A nucleoprotein (NP).

ID Screen® Influenza A Antigen Capture

Method Sandwich ELISA

Species Avian, porcine, equine

Specimens -Broncho-pulmonary liquids from pigs,horses and birds.-Bird cloacae swab samples.-Bird faeces.

Coated antibody Anti-nucleoprotein (NP) monoclonalantibody

Conjugate Anti-NP-HRP conjugate (concentrated 10X)

High sensitivity and specificity

Cost-effective for the analysis of large sample numbers

Applicable to multiple species, including horses, birds and swine - please contact iDvet for more information

Efficient and robust on complex samples (faeces and cloacae swabs) which can generate false positive results with immunochromatographic tests

Easy-to-use and rapid, with results in under 1 hour

Specifications

Ordering information

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EQUINE

Product code STREPTOS-2P

Kit format 2 plates

Reactions 192

Plate format 12 x 8-well strips

STRANGLES / STREPToCoCCuS EQui

Indirect ELISA kit for the detection of anti-Streptococcus equi in equine serum and plasma.

ID Screen® Streptococcus equi Indirect

Associated Products

Method Indirect ELISA

Species Horses

Specimens Serum and plasma

Coated antigen Recombinant SeM

Conjugate Anti-horse IgG-HRP conjugate (concentrated 10X)

Easy-to-use, standardized assay, with results in just over 2 hours

Qualitative protocol to detect previous exposure to S. equi

Quantitative protocol to assess SeM-specific antibody levels (horses with high SeM-specific serum antibodies may be predisposed to purpura when vaccinated for S. equi subsp equi with an attenuated live intranasal S. equi vaccine 1,2,3).

Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes colour upon addition of the sera

Streptococcus equi positive freeze-dried serumProduct Code MRI-STREPTO

Format 0.5 ml vial (freeze-dried)

Description Freeze-dried positive horse serum to be used as internal reference material for quality control.

Specifications

Ordering information

References

(1) Boyle, Ashley G. Streptococcus equi subspecies equi Infection (Strangles) in Horses. Vetlearn.com March 2011. Compendium: Continuing education for veterinarians. E1-8.

(2) Sweeney CR, Timoney JF, Newton JR, Hines MT. Streptococcus equi infections in horses: guidelines for treatment, control and prevention of strangles. J Vet Intern Med 2005:19:123-134.

(3) Timoney, JF. Equine strangles. Proc 45th Annu Meet AAEP 1999:31-37.

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Product code TRICHIS-MS-2P

Kit format 2 plates

Reactions 192

Plate format 12 x 8-well strips

TRiCHiNELLoSiS

Indirect multi-species ELISA kit for the detection of anti-Trichinella antibodies (T. spiralis, pseudospiralis, britovi and nativa) in serum, plasma or meat juice from pigs, wild boar and horses. Please contact IDvet for use in other species.

ID Screen® Trichinella Indirect Multi-species

Method Indirect ELISA

Species Multiple species, including pigs , wild boar, horses and others

Specimens Serum, plasma or meat juice

Coated antigen Trichinella E/S antigen

Conjugate Anti-multi-species-HRP conjugate (concentrated 10X)

Highly sensitive and specific

Applicable to multiple species, including pigs, wild boar and horses

Serum, plasma and meat juice protocols

Specifications

Ordering information

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EQUINE

Product code WNC-1P WNC-2P

Kit format 1 plate 2 plates

Reactions 96 192

Plate format 12 x 8-well strips

WEST NiLE ViRuS

Competitive ELISA kit for the detection of anti-pr-E antibodies in multiple species. The monoclonal antibody used in the kit cross-reacts with viruses from the Japanese Encephalitis Viruses (JEV) and the Tick-Borne Encephalitis Viruses (TBEV) serocomplex. Please contact IDvet for more information.

ID Screen® West Nile Competition Multi-species

Associated Products

Method Competitive ELISA

Species Equine & avian – please contact IDvet for use on other species

Specimens Serum or plasma

Coated antigen pr-E antigen

Conjugate Anti-pr-E-HRP conjugate (concentrated 10X)

Highly sensitive and specific detection of WNV antibodies

Applicable to multiple species, including horses, birds and others.

Rapid and convenient protocol, with ready-to-use reagents and results in just over 2 hours

West Nile IgG positive freeze-dried serumProduct Code MRI-WN

Format 1 ml vial (freeze-dried)

Description Freeze-dried positive horse serum to be used as internal reference material for quality control.

Specifications

Ordering information

References

(1) OIE Terrestrial Manual 2009; 2.1.20; West Nile Fever.

Horses:

(2) Van Maanen, Kees et al. Surveillance of West Nile Virus incursions in the Netherlands: Validation of antibody detecting ELISAs in horses. Oral presentation at the 2010 Epizone meeting in Saint Malo, France.

(3) Wernery, U. et al. West Nile Fever in the United Arab Emirates. Wildlife in the Middle East. Vol. 2, Issue 3, December 2007.

(4) Ziegler et al. Monitoring of West Nile Virus Infections in Germany. Zoonoses and Public Health 59 (Suppl. 2) (2012) 95-101.

Birds:

(5) Diaz-Sanchez, S. et al. Use of a multispecies competitive ELISA for investigation of exposure to West Nile virus in multiple species of captive and wild birds. Presented at the WAVLD meeting, Madrid 2009.

(6) Garcia-Bocanegra et al. Serosurvey of West Nile Virus and Other Flaviviruses of the Japanese Encephalitis Antigenic Complex in Birds from Andalusia, Southern Spain. Vector-Borne and Zoonotic Diseases. Volume 11, Number 0, 2011.

(7) Ziegler et al. Pathogenesis of West Nile virus lineage 1 and 2 in experimentally infected large falcons. Vet. Microbiol. (2012),http://dx.doi.org/10.1016/j.vetmic.2012.07.041.

(8) Yipici et al. Serologic Investigation for West Nile Virus Infection in Commerical Domestic Chickens (Gallus gallus domesticus). Journalof Animal and Veterinary Advances 11 (13):221-2214,2012

Other species:(9) Lan et. al. 2011. Serological evidence of West Nile virus in dogs and cats in China; Arch Virol (2011) 156:893-895.

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Product code WNIGM-1P WNIGM-4P

Kit format 1 plate (biwell format*)

4 plates (biwell format*)

Reactions 48 192

Plate format 6 x 16-well strips

WEST NiLE ViRuS

IgM Antibody Capture ELISA (IMAC) kit for the detection of anti-pr-E IgM antibodies in horse serum and plasma. The detection of IgM antibodies indicates recent infection and WNV circulation. The monoclonal antibody used in the kit cross-reacts with viruses from the Japanese Encephalitis Viruses (JEV) and the Tick-Borne Encephalitis Viruses (TBEV) serocomplex. Please contact IDvet for more information.

ID Screen® West Nile IgM Capture

Associated Products

Method IMAC (IgM Antibody Capture) ELISA

Species Equine

Specimens Serum and plasma

Coated antibody Anti-horse IgM polyclonal antibody

Antigen Ready-to-use WNV pr-E antigen

Conjugate Anti-WNV pr-E HRP conjugate (10X)

Protocol* (1) Sample Incubation(2) Three washes(3) WNV ready-to-use antigen incubation(4) Anti-WNV prE-HRP conjugate incubation(5) Three washes(6) Substrate Incubation: 15 min

Sensitive and specific detection of WNV igm antibodies, indicators of recent infection

Rapid and convenient : all reagents are supplied ready-to-use, samples are diluted directly in the plate (no pre-dilutions required), and all reagents may be transported at room temperature and stored at 4°C.

West Nile IgM positive freeze-dried serumProduct Code MRI-WNIGM

Format 0.5 ml vial (freeze-dried)

Description Freeze-dried IgM-positive horse serum to be used as internal reference material for quality control.

Specifications

Ordering information

References

(1) Zeller, H.G. and Schuffenecker, I. West Nile Virus: An overview of its spread in Europe and the Mediterranean basin in contrast to its spread in the Americas. Eur J Clin Microbiol Infect Dis (2004) 23:147-156

(2) OIE Terrestrial Manual 2008 ; 2.1.20; West Nile Fever.

* Samples are deposited in duplicate in adjacent even and odd-numbered wells; the WNV pr-E is added only to even-numbered wells. Results are calculated with respect to the corrected sample oD value (even well oD – odd well oD).

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AViAN

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AVIAN

Product code IBVS-5P IBVS-10P

Kit format 5 plates 10 plates

Reactions 480 960

Plate format 12 x 8-well strips

iNFECTiouS bRoNCHiTiS

Indirect ELISA for the detection of antibodies against the Infectious Bronchitis Virus (IBV) in chicken sera.

May be used to detect infection or to quantify IBV antibody levels in vaccinated birds.

Note: The same convenient protocol (buffers, incubation times and temperatures) is used for most ID Screen® poultry kits.

ID Screen® Infectious Bronchitis Indirect

Associated Products

Method Indirect ELISA

Species Chicken

Specimens Serum

Coated antigen IBV purified antigen

Conjugate Anti-chicken-HRP conjugate (concentrated 10X)

Detects all IBV variants

High specificity and very low background

Short incubation times: results in 75 minutes (30-30-15 protocol)

Comprehensive data analysis with the iDSoft™ program

Freeze-dried positive and negative reference sera available as internal controls

The stop solution no longer contains H2SO4, meaning that it is less corrosive

IBV positive freeze-dried serumProduct Code MRI-IBV

Format 0.25 ml vial (freeze-dried)

Description Freeze-dried positive chicken serum to be used as internal reference material for quality control.

SPF negative freeze-dried serumProduct Code MRINEG-BIRD

Format 0.5 ml vial (freeze-dried)

Description Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.

Specifications

Ordering information

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Product code IBVC-2P

Kit format 2 plates

Reactions 192

Plate format 12 x 8-well strips

iNFECTiouS bRoNCHiTiS

Competitive ELISA kit for the detection of anti-IBV NP antibodies in chicken serum and egg yolk.

ID Screen® Infectious Bronchitis Competition

Associated Products

Method Competitive ELISA

Species Chickens

Specimens Serum or egg yolk

Coated antigen IBV purified antigen

Conjugate Anti-IBV-HRP conjugate (concentrated 10X)

High sensitivity and specificity

Protocols for serum and egg yolk

Earlier detection of infected animals

The stop solution no longer contains H2SO4, meaning that it is less corrosive

IBV positive freeze-dried serumProduct Code MRI-IBV

Format 0.25 ml vial (freeze-dried)

Description Freeze-dried positive chicken serum to be used as internal reference material for quality control.

SPF negative freeze-dried serumProduct Code MRINEG-BIRD

Format 0.5 ml vial (freeze-dried)

Description Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.

Specifications

Ordering information

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AVIAN

Product code IBDS-5P IBDS-10P

Kit format 5 plates 10 plates

Reactions 480 960

Plate format 12 x 8-well strips

iNFECTiouS buRSAL DiSEASE / GumboRo DiSEASE

Indirect ELISA for the detection of antibodies against the Infectious Bursal Disease Virus (IBDV) in chicken sera.

May be used to monitor the flock’s immune status, to quantify IBD antibody levels pre- and post-vaccination, or to determine the best time for vaccination by detecting maternal antibodies.

Note: The same convenient protocol (buffers, incubation times and temperatures) is used for most ID Screen® poultry kits.

ID Screen® IBD Indirect

Associated Products

Method Indirect ELISA

Species Chicken

Specimens Serum

Coated antigen IBD purified antigen

Conjugate Anti-chicken-HRP conjugate (concentrated 10X)

High specificity and very low background

Short incubation times: results in 75 minutes (30-30-15 protocol)

Comprehensive data analysis with the iDSoft™ program

Freeze-dried positive and negative reference sera available as internal controls

The stop solution no longer contains H2SO4, meaning that it is less corrosive

IBD positive freeze-dried serumProduct Code MRI-IBDS

Format 0.5 ml vial (freeze-dried)

Description Freeze-dried positive chicken serum to be used as internal reference material for quality control.

SPF negative freeze-dried serumProduct Code MRINEG-BIRD

Format 0.5 ml vial (freeze-dried)

Description Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.

Specifications

Ordering information

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Product code IBDVP2-5P IBDVP2-10P

Kit format 5 plates 10 plates

Reactions 480 960

Plate format 12 x 8-well strips

iNFECTiouS buRSAL DiSEASE / GumboRo DiSEASE

Indirect ELISA for the detection of anti-IBDV VP2 antibodies in chicken sera.

May be used to quantify IBD antibody levels pre- and post-vaccination.

This test is designed to be used with animals vaccinated with recombinant VP2 vector vaccines.

ID Screen® IBD VP2

Associated Products

Method Indirect ELISA

Species Chicken

Specimens Serum

Coated antigen Recombinant VP2 antigen

Conjugate Anti-immunoglobulin-HRP conjugate (concentrated 10X)

IBD VP2 positive freeze-dried serumProduct Code MRI-IBDVP2

Format 0.5 ml vial (freeze-dried)

Description Freeze-dried VP2 positive chicken serum to be used as internal reference material for quality control.

Specifications

Ordering information

May be used with animals vaccinated with recombinant VP2 vector vaccines

High specificity and very low background

Short incubation times: results in 75 minutes(30-30-15 protocol)

Comprehensive data analysis with the iDSoft™ program

Freeze-dried positive and negative reference sera available as internal controls

The stop solution no longer contains H2SO4, meaning that it is less corrosive

SPF negative freeze-dried serumProduct Code MRINEG-BIRD

Format 0.5 ml vial (freeze-dried)

Description Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.

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AVIAN

Product code ILTS-5P ILTS-10P

Kit format 5 plates 10 plates

Reactions 480 960

Plate format 12 x 8-well strips

iNFECTiouS LARyNGoTRACHEiTiS (iLT)

Indirect ELISA for the detection of antibodies directed against the avian Infectious Laryngotracheitis Virus (ILTV) in vaccinated and/or infected chicken sera.

ID Screen® ILT Indirect

Associated Products

Method Indirect ELISA

Species Chickens

Specimens Serum

Coated antigen ILTV purified antigen

Conjugate Anti-chicken-HRP conjugate (concentrated 10x)

High specificity and analytical sensitivity

Comprehensive data analysis with the iDSoft™ program

Freeze-dried positive and negative reference sera available as internal controls

The stop solution no longer contains H2SO4, meaning that it is less corrosive

ILT positive freeze-dried serumProduct Code MRI-ILT

Format 0.5 ml vial (freeze-dried)

Description Freeze-dried positive chicken serum to be used as internal reference material for quality control.

SPF negative freeze-dried serumProduct Code MRINEG-BIRD

Format 0.5 ml vial (freeze-dried)

Description Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.

Specifications

Ordering information

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Product code FLUACA-2P FLUACA-4P FLUACA-10P

Kit format 2 plates 4 plates 10 plates

Reactions 192 384 960

Plate format 12 x 8-well strips

iNFLuENzA A

Competitive ELISA kit for the detection of anti-nucleoprotein (NP) antibodies. Detects antibodies against all Influenza A subtypes and antigenic variants thanks to the use of a monoclonal antibody against a highly conserved epitope of the Influenza A virus nucleoprotein.

ID Screen® Influenza A Antibody Competition Multi-species

Associated Products

Method Competitive ELISA

Species Multiple species, including birds, horses, swine and others

Specimens Serum and plasma

Coated antigen Influenza A nucleoprotein (NP)

Conjugate Anti-NP-HRP conjugate (concentrated 10X)

Influenza A positive freeze-dried serumProduct Code MRI-FLUACA/N1

Format 1 ml vial (freeze-dried)

Description Freeze-dried positive chicken serum to be used as internal reference material for quality control.

Specifications

Ordering information

Highly sensitive and specific

Convenient multi-species test applicable to birds, horses, swine and other species - please contact iDvet for more information

Easy-to-use, with results in under 2 hours

References

Birds: (1) Bertran, K. et al. Pathogenesis and transmissibility of highly (H7N1) and low (H7N9) pathogenic avian influenza virus infection in red-legged partridge (Alectoris rufa). Veterinary Research 2011, 42:24.(2) Dundon, W.G., Terregino, C., Tuttoilmondo V., Pizzuto, M., Busani L., Mancin, M., Cattoli, G., Capua I.. Preliminary validation of a commercial Avian Influenza N1 antibody competitive Elisa kit that can be used as part of a Diva strategy. Epizone 2007, Poland.(3) Molia, S. et al. Avian influenza in backyard poultry of the Mopti region, Mali. Trop Anim Health Prod. 2009. (4) Terregino, C. Evaluation of sensitivity and specificity of a commercial competitive avian influenza type A antibody ELISA kit, the OIE-FAO and National Reference Laboratory for Newcastle Disease and Avian Influenza, IZS delle Venezie, Legnaro (Padova), Italy.Horses: (5) Kittelberger, R., McFadden, A.M.J., Hannah, M.J., Jenner, J., Bueno, R., Vait, J., Kirkland, P.D., Delbridge, G., Heine, H.G., Selleck, P.W., Pearce, T.W., Pigott, C.J., O’Keefe, J.S., Comparative evaluation of four competitive/blocking ELISAs for the detection of influenza A antibodies in horses, Veterinary Microbiology (2010), doi:10.1016/j.vetmic.2010.08.014.Swine :(6) Busquets, N. et al. Experimental infection with H1N1 European swine influenza virus protects pigs from an infection with the 2009 pandemic H1N1 human influenza virus. Vet. Res (2010) 41:74. (7) Lange, E. et al. Pathogenesis and transmission of the novel swine-origin influenza virus A/H1N1 after experimental infection of pigs. Journal of General Virology (2009), 90, 2119-2123.Dogs:(8) De Benedictis, P. et al. A diagnostic algorithm for detection of antibodies to influenza A viruses in dogs in Italy (2006-2008). J. Vet Diagn Invest 22:914-920 (2010).

SPF negative freeze-dried serumProduct Code MRINEG-BIRD

Format 0.5 ml vial (freeze-dried)

Description Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.

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AVIAN

Kit format 2 plates

Reactions 192

Plate format 12 x 8-well strips

iNFLuENzA A

Range of competitive ELISA kits for the detection of subtype-specific Influenza antibodies in avian serum or plasma.

ID Screen® Influenza subtype-specific kits

Method Competitive ELISA

Species Avian

Specimens Serum or plasma

Coated antigen Purified native inactivated subtype-specific antigen

Conjugate Anti-subtype-HRP Mab conjugate (concentrated 10X)

Specifications

Ordering information

May be used for DIVA diagnostics (Differentiation of infected and Vaccinated Animals)*

Applicable to both domestic & wild avian species

High sensitivity and specificity : validated by oiE labs, with excellent results

Easy-to-use, with results in under 2.5 hours

* For example, if vaccination is performed using a homologous he-magglutinin to the highly pathogenic field strain, but a heterologous neuraminidase subtype, serologic surveillance can be performed by testing for antibodies against the homologous N subtype as evidence of natural infection.

Associated Products

Influenza N1 positive freeze-dried chicken serum*

Product Code MRI-FLUACA/N1

Format 1 ml vial (freeze-dried)

SPF negative freeze-dried chicken serum*Product Code MRINEG-BIRD

Format 0.5 ml vial (freeze-dried)

Name Product codeID Screen® Influenza N1 Antibody Competition

FLUACN1-2P

ID Screen® Influenza N2 Antibody Competition

FLUACN2-2P

ID Screen® Influenza N9 Antibody Competition

FLUACN9-2P

ID Screen® Influenza H5 Antibody Competition

FLUACH5-2P

ID Screen® Influenza H7 Antibody Competition

FLUACH7-2P

Influenza H7 positive freeze-dried chicken serum*

Product Code MRI-FLUACH7

Format 0.5 ml vial (freeze-dried)

* Freeze-dried chicken sera to be used as internal reference mate-rial for quality control.

References

(1) Dundon, W.G,. Terregino, C., Tuttoilmondo, V., Pizzuto, M., Busani, L., Mancin, M., Cattoli, G., Capua, I. Preliminary validation of a commercial Avian Influenza N1 antibody competitive Elisa kit that can be used as part of a Diva strategy. Poster presented at EPIZONE 2007, Poland.

(2) Bötcher, L., Schöttker-Wegner, H.H., Nöckler, A. Summary of the German presentation at the AVID Meeting (September 15-17, 2010). Use of an H5-antibody ELISAs for the serological differentiation of AIV-antibodies. Niedersächsisches Landesamt für Verbrauchschutz und Lebensmittelsicherheit Veterinärinstitut, Oldenburg, Germany.

(3) Robles, F. et al. Implementation of « DIVA » Concept with specific ELISA kits ; When Subunit H5 Avian Influenza Vaccine is Used. World Academy of Science, Engineering and Technology 65 2010.

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Method Sandwich ELISA

Species Multiple species, including birds, pigs, horses and others.

Specimens -Broncho-pulmonary liquids from pigs, horses and birds.-Bird cloacae swab samples.-Bird faeces.

Coated antibody Anti-nucleoprotein (NP) monoclonal antibody

Conjugate Anti-NP-HRP conjugate (concentrated 10X)

Product code INFLAG-2P

Kit format 2 plates

Reactions 192

Plate format 96 individual wells

Capture ELISA for the detection of the Influenza A nucleoprotein (NP).

ID Screen® Influenza A Antigen Capture

Associated Products

Specifications

Ordering information

High sensitivity and specificity

Cost-effective for the analysis of large sample numbers

Applicable to multiple species, including birds, horses and swine - please contact iDvet for more information

Efficient and robust on complex samples (faeces and cloacae swabs) which can generate false positive results with immunochromatographic tests

Easy-to-use and rapid, with results in under 1 hour

iNFLuENzA A

SPF negative freeze-dried serumProduct Code MRINEG-BIRD

Format 0.5 ml vial (freeze-dried)

Description Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.

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AVIAN

Product code MG-5P MG-10P

Kit format 5 plates 10 plates

Reactions 480 960

Plate format 12 x 8-well strips

AViAN myCoPLASmoSiS

Recombinant protein-based indirect ELISA for the detection of anti-Mycoplasma gallisepticum antibodies in chicken and turkey sera to monitor the flock’s immune status.

Positive results should be confirmed by other methods such as Rapid Slide Agglutination (RSA).

Note: The same convenient protocol (buffers, incubation times and temperatures) is used for most ID Screen® poultry kits.

ID Screen® Mycoplasma gallisepticum Indirect

Associated Products

Method Indirect ELISA

Species Chicken and turkey

Specimens Serum

Coated antigen Recombinant MG antigen

Conjugate Anti-chicken-HRP conjugate (concentrated 10X)

MG positive freeze-dried serumProduct Code MRI-MG

Format 0.5 ml vial (freeze-dried)

Description Freeze-dried positive chicken serum to be used as internal reference material for quality control.

SPF negative freeze-dried serumProduct Code MRINEG-BIRD

Format 0.5 ml vial (freeze-dried)

Description Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.

Specifications

Ordering information

The use of recombinant proteins allows for improved specificity, low background, earlier detection of seroconversion, and higher analytical sensitivity

No cross-reactions with other mycoplasmas

Short incubation times: results in 75 minutes (30-30-15 protocol)

Applicable to chickens and turkeys

Comprehensive data analysis with the iDSoft™ program

Freeze-dried positive and negative reference sera available as internal controls

Detects anti-MG antibodies further to vaccination with the 6/85 strain

The stop solution no longer contains H2SO4, meaning that it is less corrosive

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Method Indirect ELISA

Species Chicken and turkey

Specimens Serum

Coated antigen Recombinant MS antigen

Conjugate Anti-chicken-HRP conjugate (concentrated 10X)

Product code MS-5P MS-10P

Kit format 5 plates 10 plates

Reactions 480 960

Plate format 12 x 8-well strips

Recombinant protein-based indirect ELISA for the detection of anti-Mycoplasma synoviae antibodies in chicken and turkey sera to monitor the flock’s immune status.

Positive results should be confirmed by other methods such as Rapid Slide Agglutination (RSA).

Note: The same convenient protocol (buffers, incubation times and temperatures) is used for most ID Screen® poultry kits.

ID Screen® Mycoplasma synoviae Indirect

Associated Products

Specifications

Ordering information

AViAN myCoPLASmoSiS

MS positive freeze-dried serumProduct Code MRI-MS

Format 1 ml vial (freeze-dried)

Description Freeze-dried positive chicken serum to be used as internal reference material for quality control.

SPF negative freeze-dried serumProduct Code MRINEG-BIRD

Format 0.5 ml vial (freeze-dried)

Description Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.

The use of recombinant proteins allows for improved specificity, low background, earlier detection of seroconversion, and higher analytical sensitivity

No cross-reactions with other mycoplasmas

Short incubation times: results in 75 minutes (30-30-15 protocol)

Applicable to chickens and turkeys

Comprehensive data analysis with the iDSoft™ program

Freeze-dried positive and negative reference sera available as internal controls

The stop solution no longer contains H2SO4, meaning that it is less corrosive

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AVIAN

Product code MG/MS-5P MG/MS-10P

Kit format 5 plates 10 plates

Reactions 480 960

Plate format 12 x 8-well strips

AViAN myCoPLASmoSiS

Recombinant protein-based indirect ELISA for the detection of anti-Mycoplasma gallisepticum and Mycoplasma synoviae antibodies in chicken and turkey sera to monitor the flock’s immune status.

Positive results should be confirmed using the separate ID Screen® MG or MS Indirect ELISAs, and then by other methods such as Rapid Slide Agglutination (RSA).

Note: The same convenient protocol (buffers, incubation times and temperatures) is used for most ID Screen® poultry kits.

ID Screen® MG/MS Indirect

Associated Products

Method Indirect ELISA

Species Chicken and turkey

Specimens Serum

Coated antigen MG and MS recombinant antigens

Conjugate Anti-chicken-HRP conjugate (concentrated 10X)

SPF negative serumProduct Code MRINEG-BIRD

Format 1 ml vial (freeze-dried)

Description Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.

Specifications

Ordering information

MS positive serumProduct Code MRI-MS

Format 1 ml vial (freeze-dried)

Description Freeze-dried positive chicken serum to be used as internal reference material for quality control.

MG positive serumProduct Code MRI-MG

Format 0.5 ml vial (freeze-dried)

Description Freeze-dried positive chicken serum to be used as internal reference material for quality control.

The use of recombinant proteins allows for improved specificity, low background, earlier detection of seroconversion, and higher analytical sensitivity

No cross-reactions with other mycoplasmas

Short incubation times: results in 75 minutes (30-30-15 protocol)

Applicable to chickens and turkeys

Comprehensive data analysis with the iDSoft™ program

Freeze-dried positive and negative reference sera available as internal controls

The stop solution no longer contains H2SO4, meaning that it is less corrosive

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AVIAN

Product code NDVS-5P NDVS-10P

Kit format 5 plates 10 plates

Reactions 480 960

Plate format 12 x 8-well strips

Indirect ELISA for the detection of antibodies against the Newcastle Disease Virus (NDV) in chicken sera.

May be used to detect infection or to quantify NDV antibody levels in vaccinated birds.

Note: The same convenient protocol (buffers, incubation times and temperatures) is used for most ID Screen® poultry kits.

ID Screen® Newcastle Disease Indirect

Associated Products

Method Indirect ELISA

Species Chicken

Specimens Serum

Coated antigen NDV purified antigen

Conjugate Anti-chicken-HRP conjugate (concentrated 10X)

NDV positive freeze-dried serumProduct Code MRI-NDV

Format 0.5 ml vial (freeze-dried)

Description Freeze-dried positive chicken serum to be used as internal reference material for quality control.

SPF negative freeze-dried serumProduct Code MRINEG-BIRD

Format 0.5 ml vial (freeze-dried)

Description Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.

Specifications

Ordering information

High specificity and very low background

Short incubation times: results in 75 minutes (30-30-15 protocol)

Excellent correlation with hemagglutination inhibition (Hi)

Comprehensive data analysis with the iDSoft™ program

Freeze-dried positive and negative reference sera available as internal controls

The stop solution no longer contains H2SO4, meaning that it is less corrosive

NEWCASTLE DiSEASE

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Product code NDVC-2P NDVC-5P NDVC-10P

Kit format 2 plates 5 plates 10 plates

Reactions 192 480 960

Plate format 12 x 8-well strips

Competitive ELISA kit for the detection of anti-NDV nucleoprotein antibodies in serum and egg yolk (domestic and wild avian species).

Applicable to both domestic and wild avian species, this test may be used to determine the immune status of individuals, flocks or populations.

ID Screen® Newcastle Disease Competition

Associated Products

Method Competitive ELISA

Species Avian (domestic and wild species)

Specimens Serum, plasma and egg yolk

Coated antigen NDV-purified antigen

Conjugate Anti-NDV-HRP conjugate (concentrated 10X)

NDV positive freeze-dried serumProduct Code MRI-NDV

Format 0.5 ml vial (freeze-dried)

Description Freeze-dried positive chicken serum to be used as internal reference material for quality control.

SPF negative freeze-dried serumProduct Code MRINEG-BIRD

Format 0.5 ml vial (freeze-dried)

Description Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.

Specifications

Ordering information

Multi-species test: the only commercial ELiSA applicable to both domestic and wild avian species

High sensitivity and specificity: • Earlier detection of seroconversion (7 dpi) compared with indirect ELiSAs • High analytical sensitivity • Detection of all strains • No cross-reactions with Avian Paramyxoviruses 3 (APmV-3).

Flexible and rapid protocol : applicable to serum, plasma and egg yolk samples, and results are obtained in just 70 minutes.

Used worldwide in government monitoring programs

The stop solution no longer contains H2SO4, meaning that it is less corrosive

NEWCASTLE DiSEASE

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AVIAN

Product code SALS-5P SALS-10P

Kit format 5 plates 10 plates

Reactions 480 960

Plate format 12 x 8-well strips

Indirect ELISA for the detection of anti-Salmonella group B and D antibodies in chicken sera.

Note: The same convenient protocol (buffers, incubation times and temperatures) is used for most ID Screen® poultry kits.

ID Screen® Avian Salmonella Indirect – Groups B & D

Associated Products

Method Indirect ELISA

Species Chicken

Specimens Serum

Coated antigen Purified Salmonella LPS

Conjugate Anti-chicken IgG-HRP conjugate (concentrated 10X)

SAL positive freeze-dried serumProduct Code MRI-SALS

Format 0.5 ml vial (freeze-dried)

Description Freeze-dried positive chicken serum to be used as internal reference material for quality control.

SPF negative freeze-dried serumProduct Code MRINEG-BIRD

Format 0.5 ml vial (freeze-dried)

Description Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.

Specifications

Ordering information

Very low background

Detects antibodies directed against Salmonella enteritidis (Se), S. thyphimurium (St), and the other avian salmonellas belonging to groups B and D.

Short incubation times with results in 75 minutes

Comprehensive data analysis with the iDSoft™ program

Freeze-dried positive and negative reference sera available as internal controls

The stop solution no longer contains H2SO4, meaning that it is less corrosive

SALmoNELLoSiS

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Product code WNC-1P WNC-2P

Kit format 1 plate 2 plates

Reactions 96 192

Plate format 12 x 8-well strips

WEST NiLE ViRuS

Competitive ELISA kit for the detection of anti-pr-E antibodies in multiple species. The monoclonal antibody used in the kit cross-reacts with viruses from the Japanese Encephalitis Viruses (JEV) and the Tick-Borne Encephalitis Viruses (TBEV) serocomplex. Please contact IDvet for more information.

ID Screen® West Nile Competition Multi-species

Associated Products

Method Competitive ELISA

Species Multiple species, including birds, horses, and others.

Specimens Serum or plasma

Coated antigen pr-E antigen

Conjugate Anti-pr-E-HRP conjugate (concentrated 10X)

Specifications

Ordering information

Highly sensitive and specific detection of WNV antibodies

Multi-species test for horses and birds

Rapid and convenient protocol, with ready-to-use reagents and results in just over 2 hours

References

(1) OIE Terrestrial Manual 2009; 2.1.20; West Nile Fever.

Birds:

(2) Diaz-Sanchez, S. et al. Use of a multispecies competitive ELISA for investigation of exposure to West Nile virus in multiple species of captive and wild birds. Presented at the WAVLD meeting, Madrid 2009.

(3) Garcia-Bocanegra et al. Serosurvey of West Nile Virus and Other Flaviviruses of the Japanese Encephalitis Antigenic Complex in Birds from Andalusia, Southern Spain. Vector-Borne and Zoonotic Diseases. Volume 11, Number 0, 2011.

(4) Ziegler et al. Pathogenesis of West Nile virus lineage 1 and 2 in experimentally infected large falcons. Vet. Microbiol. (2012),http://dx.doi.org/10.1016/j.vetmic.2012.07.041.

(5) Yipici et al. Serologic Investigation for West Nile Virus Infection in Commerical Domestic Chickens (Gallus gallus domesticus). Journalof Animal and Veterinary Advances 11 (13):221-2214,2012

Horses:

(6) Van Maanen, Kees et al. Surveillance of West Nile Virus incursions in the Netherlands: Validation of antibody detecting ELISAs in horses. Oral presentation at the 2010 Epizone meeting in Saint Malo, France.

(7) Wernery, U. et al. West Nile Fever in the United Arab Emirates. Wildlife in the Middle East. Vol. 2, Issue 3, December 2007.

(8) Ziegler et al. Monitoring of West Nile Virus Infections in Germany. Zoonoses and Public Health 59 (Suppl. 2) (2012) 95-101.

Other species:(9) Lan et. al. 2011. Serological evidence of West Nile virus in dogs and cats in China; Arch Virol (2011) 156:893-895.

SPF negative freeze-dried serumProduct Code MRINEG-BIRD

Format 0.5 ml vial (freeze-dried)

Description Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.

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SWiNE

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SWINE

Product code APPS-2P

Kit format 2 plates

Reactions 192

Plate format 12 x 8-well strips

ACTiNobACiLLuS PLEuRoPNEumoNiAE

Indirect ELISA kit for the detection of antibodies against APP serotypes 1 through 12 in swine serum, plasma and meat juice.

ID Screen® APP Screening Indirect (serotypes 1 through 12)

Method Indirect ELISA

Species Swine

Specimens Serum, plasma or meat juice

Coated antigen APP antigens

Conjugate Anti-swine IgG–HRP conjugate (concentrated 10X)

Specifications

Ordering information

Screening of 12 APP serotypes in a single test. Serotyping may be performed on positive samples using the iD Screen® serotyping ELiSAs (opposite page).

Fast and easy-to-use, no sample pre-dilution required, and results in just 75 minutes

The same straightforward protocol is used for all ID Screen® APP ELISAs, making it easier to use several tests at once

Serum, plasma and meat juice protocols

References

Pourquier, P., Lesceu, S. Use of the ID Screen APP indirect ELISA range for quick identification and serotyping of Actinobacillus pleuropneumoniae in domestic pigs. Poster presented at the 6th International Symposium on Emerging and Re-emerging Pig Diseases, June 2011, Madrid, Spain.

Associated Products

APP positive freeze-dried sera

Product Code MRI-APP-1-9-11 MRI-APP-2 MRI-APP-3-8 MRI-APP-4-7 MRI-APP-5 MRI-APP-6 MRI-APP-10 MRI-APP-12

Format 0.25 ml vial (freeze-dried)

0.25 ml vial (freeze-dried)

1 ml vial (freeze-dried)

0.25 ml vial (freeze-dried)

0.25 ml vial (freeze-dried)

1 ml vial (freeze-dried)

0.25 ml vial (freeze-dried)

0.25 ml vial (freeze-dried)

Description Freeze-dried positive swine serum to be used as internal reference material for quality control.

APP negative freeze-dried serum Product Code MRINEG-APP

Format 0.5 ml vial (freeze-dried)

Description Freeze-dried negative swine serum to be used as internal reference material for quality control.

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References

Pourquier, P., Lesceu, S. Use of the ID Screen APP indirect ELISA range for quick identification and serotyping of Actinobacillus pleuropneumoniae in domestic pigs. Poster presented at the 6th International Symposium on Emerging and Re-emerging Pig Diseases, June 2011, Madrid, Spain.

Kit format 2 plates

Reactions 192

Plate format 12 x 8-well strips

ACTiNobACiLLuS PLEuRoPNEumoNiAE

Comprehensive range of indirect ELISAs for the detection of serotype-specific APP antibodies in swine serum, plasma and meat juice.

ID Screen® APP serotyping kits

Associated Products

Method Indirect ELISA

Species Swine

Specimens Serum, plasma or meat juice

Coated antigen APP serotype specific antigens

Conjugate Anti-swine IgG – HRP conjugate (concentrated 10X)

APP positive freeze-dried sera

Product Code MRI-APP-1-9-11 MRI-APP-2 MRI-APP-3-8 MRI-APP-4-7 MRI-APP-5 MRI-APP-6 MRI-APP-10 MRI-APP-12

Format 0.25 ml vial (freeze-dried)

0.25 ml vial (freeze-dried)

1 ml vial (freeze-dried)

0.25 ml vial (freeze-dried)

0.25 ml vial (freeze-dried)

1 ml vial (freeze-dried)

0.25 ml vial (freeze-dried)

0.25 ml vial (freeze-dried)

Description Freeze-dried positive swine serum to be used as internal reference material for quality control.

Specifications

Ordering information

High serotype-specificity and sensitivity

Easy-to-use, as there is no sample pre-dilution step, and results are obtained in just 75 minutes

The same fast and convenient protocol is used for the entire range of ID Screen® APP ELISAs, making it easier to use several tests at once

Name Product codeID Screen® APP 1-9-11 Indirect APPS1-9-11-2P

ID Screen® APP 2 Indirect APPS2-2P

ID Screen® APP 2-6 Indirect APPS2-6-2P

ID Screen® APP 2-6-12 Indirect APPS2-6-12-2P

ID Screen® APP 3-6-8 Indirect APPS3-6-8-2P

ID Screen® APP 4-7 Indirect APPS4-7-2P

ID Screen® APP 5 Indirect APPS5-2P

ID Screen® APP 8 Indirect APPS8-2P

ID Screen® APP 10 Indirect APPS10-2P

ID Screen® APP 12 Indirect APPS12-2P

APP negative freeze-dried serum Product Code MRINEG-APP

Format 0.5 ml vial (freeze-dried)

Description Freeze-dried negative swine serum to be used as internal reference material for quality control.

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SWINE

Product code AUJESZKYGB-4P

Kit format 4 plates

Reactions 384

Plate format 12 x 8-well strips

AuJESzky’S DiSEASE / PSEuDoRAbiES

Competitive ELISA for the detection of anti-gB antibodies. Detects antibodies against all Aujeszky’s Disease Virus (ADV) strains further to natural infection or vaccination.

ID Screen® Aujeszky gB Competition

Associated Products

Method Competitive ELISA

Species Swine (domestic pigs and wild boar)

Specimens Serum or plasma

Coated antigen Antigenic suspension of the Aujeszky Disease Virus (ADV)

Conjugate Anti-gB-HRP conjugate (concentrated 10X)

Specifications

Ordering information

High sensitivity and specificity

Meets OIE analytical sensitivity requirements1: calibrated against the OIE ADV-1 reference serum

Convenient and flexible : short (results within 2 hours) or overnight protocols

Automatable for high throughput testing

Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes colour upon addition of the sera

ADV positive freeze-dried serum (vaccinated animal)Product Code MRI-AUJGB

Format 1 ml vial (freeze-dried)

Description Freeze-dried positive porcine serum from a vaccinated animal. To be used as internal reference material for quality control. This serum gives a negative result on the ID Screen® Aujeszky gE Competition ELISA.

References

(1) OIE Terrestrial Manual 2008; 2.1.2; Aujeszky’s Disease.

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Method Competitive ELISA

Species Swine (domestic pigs and wild boar)

Specimens Serum or plasma

Coated antigen Antigenic suspension of the Aujeszky Disease Virus (ADV)

Conjugate Ready to use anti-gE-HRP conjugate

Product code AUJESZKYGE-4P

Kit format 4 plates

Reactions 384

Plate format 12 x 8-well strips

AuJESzky’S DiSEASE / PSEuDoRAbiES

Competitive ELISA for the detection of anti-gE antibodies in swine serum and plasma. Detects naturally-infected animals in a vaccinated population, provided that animals are vaccinated with a vaccine that does not express the gE protein. For use in ADV vaccination-eradication programs in combination with gE-deleted vaccines.

ID Screen® Aujeszky gE Competition

Specifications

Ordering information

Sensitive and specific detection of anti-gE antibodies

Meets OIE analytical sensitivity requirements1: calibrated against the OIE International Standard Reference Serum

May be used as a DIVA test (Differentiation of Infected and Vaccinated Animals)

Convenient and flexible protocol : ready-to-use conjugate, with short and overnight protocols

Automatable for high throughput testing

Associated Products

ADV positive freeze-dried serum (naturally-infected animal)Product Code MRI-AUJGE

Format 1 ml vial (freeze-dried)

Description Freeze-dried positive porcine serum from a naturally-infected animal. To be used as internal reference material for quality control. This serum is also positive on the ID Screen® Aujeszky gB Competition ELISA.

References

(1) OIE Terrestrial Manual 2008; 2.1.2; Aujeszky’s Disease.

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SWINE

Method Indirect ELISA

Species Swine (domestic pigs and wild boar)

Specimens Serum, plasma, meat juice or blood filter paper samples

Coated antigen Mix of P32, P62 and P72recombinant proteins

Conjugate Anti-multi-species-HRP concentratedconjugate (10X)

AFRiCAN SWiNE FEVER

Multi-antigen indirect ELISA kit for the detection of antibodies against P32, P62 and P72 of the ASFV in porcine serum, plasma, meat juice or blood filter paper samples.

ID Screen® African Swine Fever Indirect

Specifications

Ordering information

The only commercial ELISA based on 3 recombinant antigens : P32, P62, and P72

High sensitivity and specificity, with clear separation of positive and negative results

Convenient and versatile, with ready-to-use components and protocols for serum, plasma, meat juice and blood filter paper samples.

Blood filter paper samples may be tested in 96-well deep-well plates, making sample processing faster and less prone to mix-ups

Associated Products

ASF positive freeze-dried serumProduct Code MRI-ASF

Format 0.5 ml vial (freeze-dried)

Description Freeze-dried positive porcine serum to be used as internal reference material for quality control.

* Each sample is tested in duplicate in a coated well (even columns) and in an adjacent non-coated well (odd columns). Results are calculated with respect to the corrected sample oD value (even well oD – odd well oD).

Confirmation format*

Product code ASFB-4P

Kit format 4 plates (bi-well)

Reactions 192

Plate format 6 x 16 well strips

Screening format

Product code ASFS-2P ASFS-4P

Kit format 2 plates 4 plates

Reactions 192 384

Plate format 12 x 8-well strips

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bRuCELLoSiS

ID Screen® Brucellosis Serum Indirect Multi-species

Indirect ELISA for the detection of antibodies against Brucella abortus, melitensis or suis in bovine, ovine, caprine and porcine serum and plasma (individual samples or pools of up to 10).

Calibrated according to OIE specifications and Annex C of the European directive CEE 64/432 to correctly detect the OIEELISASPISS standard serum.

Straightforward, versatile test, with short and overnight incubations for individual serum or plasma samples, or pools of up to 10 samples.

Product code BRUS-MS-4P BRUS-MS-10P

Kit format 4 plates 10 plates

Reactions 384 960

Plate format 12 x 8-well strips

Method Indirect ELISA

Species Bovine, ovine, caprine and porcine. Please contact IDvet for use in other species.

Specimens Individual serum or plasma samples, or pools of up to 10 samples.

Coated antigen Purified Brucella LPS

Conjugate Anti-multi-species-IgG-HRP conjugate (concentrated 10X)

Specifications

Ordering information

References

(1) OIE Terrestrial Manual 2009; 2.4.3; Bovine brucellosis.

(2) Annex C of the European directive CEE 64/432

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SWINE

Product code CSFE2C-4P

Kit format 4 plates

Reactions 384

Plate format 12 x 8-well strips

CLASSiCAL SWiNE FEVER / HoG CHoLERA

Competitive ELISA kit for the detection of anti-E2 antibodies in swine serum or plasma.

ID Screen® Classical Swine Fever E2 Competition

Associated Products

Method Competitive ELISA

Species Swine (domestic pigs and wild boar)

Specimens Serum or plasma

Coated antigen Recombinant E2 glycoprotein

Conjugate Ready-to-use anti-E2-HRP conjugate

Specifications

Ordering information

Sensitive and specific detection of anti-E2 antibodies

Fast and convenient protocol : ready-to-use conjugate, and results in 90 minutes.

Classical Swine Fever positivefreeze-dried serumProduct Code MRI-CSFE2

Format 1 ml vial (freeze-dried)

Description Freeze-dried highly positive swine serum to be used as internal reference material for quality control.

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Method Indirect ELISA

Species Swine and other species. Please contact IDvet for more information.

Specimens Serum or plasma

Coated antigen Recombinant genotype 3 capsid

Conjugate Anti-multi-species IgG-HRP conjugate (concentra-ted 10X)

HEPATiTiS E

Indirect ELISA for the detection of anti-Hepatitis E antibodies in serum and plasma from swine and other species. The use of genotype 3 antigens makes the test ideally suited for use in swine, as genotype 3 is the genotype which infects pigs (HEV ELISAs for humans use genotype 1 antigens).

ID Screen® Hepatitis E Indirect Multi-species

Specifications

High sensitivity and specificity in swine, thanks to the use of genotype 3 antigens

Applicable to multiple species, and validated on swine. Please contact IDvet for more information

Straightforward and rapid, with results in 90 minutes

References

Eiden M., Schlosser M., Groschup J. (Friedrich-Loeffler-Institut, Insel Riems, Germany), Pourquier P., Comtet L. (IDvet). Preliminary validation data of a commercial multi-species ELISA kit for detection of anti-Hepatitis E Virus antibodies in swine. Poster presented at the 6th International Symposium on Emerging and Re-emerging Pig Diseases, June 2011, Madrid, Spain.

Product code HEVB-MS-4P

Kit format 4 plates

Reactions 192 (Biwell format*)

Plate format 6 x 16-well strips

Ordering information

* Each sample is tested in duplicate in a coated well (even columns) and in an ad-jacent non-coated well (odd columns). Results are calculated with respect to the corrected sample oD value (even well oD – odd well oD).

Associated Products

HEV positive freeze-dried serumProduct Code MRI-HEV

Format 1 ml vial (freeze-dried)

Description Freeze-dried positive swine serum to be used as internal reference material for quality control.

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SWINE

Product code FLUACA-2P FLUACA-4P FLUACA-10P

Kit format 2 plates 4 plates 10 plates

Reactions 192 384 960

Plate format 12 x 8-well strips

iNFLuENzA A

Competitive ELISA kit for the detection of anti-nucleoprotein (NP) antibodies. Detects antibodies against all Influenza A subtypes and antigenic variants thanks to the use of a monoclonal antibody against a highly conserved epitope of the Influenza A virus nucleoprotein.

ID Screen® Influenza A Antibody Competition Multi-species

Method Competitive ELISA

Species Multiple species, including swine, birds, horses and others

Specimens Serum and plasma

Coated antigen Influenza A nucleoprotein (NP)

Conjugate Anti-NP-HRP conjugate (concentrated 10X)

Specifications

Ordering information

Highly sensitive and specific

Convenient multi-species test applicable to birds, horses, swine and other species - please contact IDvet for more information

Cost-effective for the analysis of large sample numbers, particularly in comparison with Haemagglutination Inhibition (HI)

Easy-to-use, with results in less than 2 hours

References

Swine :(1) Busquets, N. et al. Experimental infection with H1N1 European swine influenza virus protects pigs from an infection with the 2009 pandemic H1N1 human influenza virus. Vet. Res (2010) 41:74. (2) Lange, E. et al. Pathogenesis and transmission of the novel swine-origin influenza virus A/H1N1 after experimental infection of pigs. Journal of General Virology (2009), 90, 2119-2123. Horses: (3) Kittelberger, R., McFadden, A.M.J., Hannah, M.J., Jenner, J., Bueno, R., Vait, J., Kirkland, P.D., Delbridge, G., Heine, H.G., Selleck, P.W., Pearce, T.W., Pigott, C.J., O’Keefe, J.S., Comparative evaluation of four competitive/blocking ELISAs for the detection of influenza A antibodies in horses, Veterinary Microbiology (2010), doi:10.1016/j.vetmic.2010.08.014.Birds: (4) Bertran, K. et al. Pathogenesis and transmissibility of highly (H7N1) and low (H7N9) pathogenic avian influenza virus infection in red-legged partridge (Alectoris rufa). Veterinary Research 2011, 42:24.(5) Dundon, W.G., Terregino, C., Tuttoilmondo V., Pizzuto, M., Busani L., Mancin, M., Cattoli, G., Capua I.. Preliminary validation of a commercial Avian Influenza N1 antibody competitive Elisa kit that can be used as part of a Diva strategy. Epizone 2007, Poland.(6) Molia, S. et al. Avian influenza in backyard poultry of the Mopti region, Mali. Trop Anim Health Prod. 2009. (7) Terregino, C. Evaluation of sensitivity and specificity of a commercial competitive avian influenza type A antibody ELISA kit, the OIE-FAO and National Reference Laboratory for Newcastle Disease and Avian Influenza, IZS delle Venezie, Legnaro (Padova), Italy.Dogs:(8) De Benedictis, P. et al. A diagnostic algorithm for detection of antibodies to influenza A viruses in dogs in Italy (2006-2008). J. Vet Diagn Invest 22:914-920 (2010).

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Method Sandwich ELISA

Species Multiple species, including swine, birds, horses and others.

Specimens -Broncho-pulmonary liquids from pigs, horses and birds.-Bird cloacae swab samples.-Bird faeces.

Coated antibody Anti-nucleoprotein (NP) monoclonal antibody

Conjugate Anti-NP-HRP conjugate (concentrated 10X)

Product code INFLAG-2P

Kit format 2 plates

Reactions 192

Plate format 96 individual wells

iNFLuENzA A

Capture ELISA for the detection of the Influenza A nucleoprotein (NP).

ID Screen® Influenza A Antigen Capture

Specifications

Ordering information

High sensitivity and specificity

Cost-effective for the analysis of large sample numbers

Applicable to multiple species, including birds, horses and swine - please contact IDvet for more information

Efficient and robust on complex samples (faeces and cloacae swabs) which can generate false positive results with immunochromatographic tests

Easy-to-use and rapid, with results in less than 1 hour

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SWINE

Method Indirect ELISA

Species SwinePlease contact IDvet for use in other species

Specimens Serum, plasma or meat juice

Coated antigen Purified MAC extract

Conjugate Anti-multi-species IgG-HRP conjugate (concentrated 10X)

Product code MA-MS-4P

Kit format 4 plates

Reactions 384

Plate format 12 x 8-well strips

myCobACTERium AVium

Indirect multi-species ELISA for the detection of anti-Mycobacterium avium complex (MAC) antibodies in sera and plasma from swine.

ID Screen® Mycobacterium avium Indirect Multi-species

Specifications

Ordering information

Excellent specificity

This test is designed to detect circulation of M. avium subsp. hominissuis on the herd level (low test sensitivity for individuals)

Cost-effective for high throughput testing, notably in comparison to the incision of lymph nodes at slaughter

Convenient protocol, with ready-to-use components and results in 2 hours.

(1) Pourquier, P., Lesceu S. Preliminary validation of the ID Screen® Mycobacterium avium Multi-species Indirect ELISA. Poster presented at the AHVLA International Conference 2011 - Animal Diseases and their Consequences, Royal Holloway, England.

(2) Shade B et al. Diagnostic investigations on mycobacterial abortion in a sow herd. Poster presented at the 21st IPVS Congress, Vancouver, Canada, July, 2010.

References

Associated Products

Mycobacterium avium positive freeze-dried serumProduct Code MRI-MA

Format 1 ml vial (freeze-dried)

Description Freeze-dried positive swine serum to be used as internal reference material for quality control.

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Method Indirect ELISA

Species Swine

Specimens Serum or plasma

Coated antigen Recombinant P46 of M. hyopneumoniae

Conjugate Anti-swine IgG-HRP conjugate (concentrated 10X)

Product code MHYOPS-2P

Kit format 2 plates

Reactions 192

Plate format 12 x 8-well strips

myCoPLASmA HyoPNEumoNiAE / ENzooTiC PNEumoNiAE / myCoPLASmAL PNEumoNiA oF SWiNE

Indirect ELISA kit for the detection of anti-P46 antibodies in swine serum and plasma.

ID Screen® Mycoplasma hyopneumoniae Indirect

Specifications

Ordering information

Accurate and reliable, with high specificity and sensitivity

Easy-to-use : results in 90 minutes

Associated Products

Mycoplasma hyopneumoniae positive freeze-dried serumProduct Code MRI-MHYOP

Format 1 ml vial (freeze-dried)

Description Freeze-dried positive swine serum to be used as internal reference material for quality control.

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SWINE

Method Competitive ELISA

Species Swine

Specimens Serum

Coated antigen Purified PRRSV antigen

Conjugate Anti-nucleoprotein-HRP conjugate (concentrated 10X)

Product code PRRS-4P PRRS-10P

Kit format 4 plates 10 plates

Reactions 384 960

Plate format 12 x 8-well strips

PoRCiNE REPRoDuCTiVE AND RESPiRAToRy SyNDRomE (PRRS)

Competitive ELISA for the detection of PRRSV anti-nucleoprotein (ORF7) antibodies in swine serum samples. Detects antibodies against both the US and European strains.

ID Screen® PRRS Competition

Specifications

Ordering information

Detection of both US and European strains

Excellent specificity and sensitivity

Convenient protocol, with ready-to-use components and results in 2 hours

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Product code SVDC-4P

Kit format 4 plates

Reactions 384

Plate format 12 x 8-well strips

SWiNE VESiCuLAR DiSEASE

5B7 Mab Competitive ELISA1,2 for the detection of antibodies directed against the SVD virus in porcine serum or plasma.

ID Screen® Swine Vesicular Disease Competition

Method Competitive ELISA

Species Swine

Specimens Serum or plasma

Coated antigen SVDV purified antigen

Conjugate Mab 5B7-HRP conjugate (concentrated 10X)

Specifications

Ordering information

High sensitivity and specificity, with clear separation of positive and negative results

Calibrated to detect the Primary Reference Serum EU-RS4, provided by the OIE Reference Laboratory, Pirbright, UK2

Convenient and rapid test, with ready-to-use components and results within 2 hours.

(1) Brocchi, E., Berlinzani, A., Gamba, D. & Simone, F. (1995). Development of two novel monoclonal antibody-based ELISAs for the detection of antibodies and the identification of swine isotypes against swine vesicular disease virus. J. Virol.Methods, 52, 155-67.

(2) OIE Terrestrial Manual 2013; 2.8.9; Swine Vesicular Disease.

References

Associated Products

SVD positive freeze-dried serumProduct Code MRI-SVD

Format 0.5 ml vial (freeze-dried)

Description Freeze-dried positive swine serum to be used as internal reference material for quality control.

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SWINE

Method Indirect ELISA

Species Multiple species, including ruminants, swine, dogs, cats and others

Specimens Serum, plasma or meat juice

Coated antigen Toxoplasma gondii P30 antigen

Conjugate Anti-multi-species IgG-HRP conjugate (concentrated 10X)

Product code TOXOS-MS-2P

Kit format 2 plates

Reactions 192

Plate format 12 x 8-well strips

ToxoPLASmoSiS

Indirect multi-species ELISA for the detection of anti-Toxoplasma gondii antibodies in serum, plasma or meat juice from multiple species, including ruminants, swine, dogs and cats.

ID Screen® Toxoplasmosis Indirect Multi-species

Specifications

Ordering information

Easy-to-use, with results in 90 minutes

Versatile protocol for serum, plasma or meat juice

Applicable to multiple species, including ruminants, swine, dogs and cats

Associated Products

Toxoplasmosis positive freeze-dried serumProduct Code MRI-TOXO

Format 1 ml vial (freeze-dried)

Description Freeze-dried positive porcine serum (wild boar) to be used as internal reference material for quality control.

References

Swine(1) Hotea, I. et al. Seroprevalence of Toxoplasma gondii infection in pigs reared in intensive system from Timis country. Lucrari Stiintice Medicina veterinara Vol. XLIII(1), 2010, Timisoara.(2) Görlich, K. et al. Validation, optimisation and standardisation of an automated test system for monitoring and surveillance of Toxoplasmosa gondii in pigs. 2010. Poster presented at Nationales Zoonose Symposium, Berlin, 2009.(3) Gómez-Laguna, J. et al. Seroprevalence of zoonotic diseases in Iberian pigs. SUIS N° 74, Enero/Febrero 2011.Sheep(4) Proctor AF et al. Detection of antibodies to Toxoplasma gondii in serum from experimentally infected pregnant ewes. University College Dublin, 2008(5) Mangili, P.M. et al. Development and evaluation of the performance of an in-house ELISA to be used for the indirect diagnosis of Toxoplasmosis in sheep. Poster presented at the SIDILV meeting in Parma, Italy, 2009.Deer(6) Gaffuri, A., et al. Toxoplasma gondii in wild boar and roe deer in Northern Italy: serosurvey and PCR-RFLP. 9th Ewda Conference, 2010.(7) Renzi, M. et al. Serological investigation on the spread of Toxoplasma gondii in roe deer (Capreolus capreolus) Emilia-Romagna. Poster presented at the 3rd National SIEF Congress, Torino, 2009.Dogs and cats(8) Scarpulla, M. et al. Comparison of indirect immunofluorescence and ID Screen® Toxoplasmosis indirect ELISA for the detection of antibodies against Toxoplasma gondii in cat and dog sera. Poster presented at the WAVLD meeting, Madrid, 2009.

Wild & domestic animals(9) Roqueplo, C. et al. Toxoplasma gondii in wild and domestic animals from New Caledonia. Parasite, 2011, 18, 345-348.

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Product code TRICHIS-MS-2P

Kit format 2 plates

Reactions 192

Plate format 12 x 8-well strips

TRiCHiNELLoSiS

Indirect multi-species ELISA kit for the detection of anti-Trichinella antibodies (T. spiralis, pseudospiralis, britovi and nativa) in serum, plasma or meat juice from pigs, wild boar and horses. Please contact IDvet for use with other species.

ID Screen® Trichinella Indirect Multi-species

Associated Products

Method Indirect ELISA

Species Multiple species, including pigs , wild boar, horses and others

Specimens Serum, plasma or meat juice

Coated antigen Trichinella E/S antigen

Conjugate Anti-multi-species-HRP conjugate (concentrated 10X)

Specifications

Ordering information

Highly sensitive and specific

Applicable to multiple species, including pigs, wild boars and horses

Serum, plasma and meat juice protocols

Trichinellosis positive freeze-dried serumProduct Code MRI-TRICHI

Format 1 ml vial (freeze-dried)

Description Freeze-dried positive porcine (wild boar) serum to be used as internal reference material for quality control.

Trichinellosis negative freeze-dried serumProduct Code MRINEG-TRICHI

Format 1 ml vial (freeze-dried)

Description Freeze-dried negative porcine (wild boar) serum to be used as internal reference material for quality control.

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ComPANioN ANimALS

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ComPANioN ANimALS

Method Indirect ELISA

Species Dogs

Specimens Serum and plasma

Coated antigen L. infantum purified antigen

Conjugate Anti-canine IgG–HRP conjugate (concentrated 10X)

Product code LEISHS-2P

Kit format 2 plates

Reactions 192

Plate format 12 x 8-well strips

CANiNE LEiSHmANiASiS

Indirect ELISA kit for the detection of anti-Leishmania infantum antibodies in canine serum and plasma.

ID Screen® Leishmaniasis Indirect

Specifications

Ordering information

Particularly rapid, notably in comparison with indirect immunofluorescence

Improved specificity thanks to the use of a L. infantum purified antigen

Easy-to-use, with results in 90 minutes

Associated Products

Product Code IFI-LEISH

Format 10 slides, 10 reactions per slide.

Description Slides for the serodiagnosis of Leishmania by indirect immunofluorescence (IIF) in dog sera.

Leishmania infantum Immunofluorescent Slides

Leishmania infantum positive freeze-dried serumProduct Code MRI-LEISH

Format 1 ml vial (freeze-dried)

Description Freeze-dried, highly-positive serum from a naturally-infected dog.

Produces a positive reaction when analysed by ELISA or by IFAT (Indirect Fluorescent Antibody Test)

To be used as internal reference material for quality control.

Leishmania infantum negative freeze-dried serumProduct Code MRINEG-LEISH

Format 2 ml vial (freeze-dried)

Description Freeze-dried negative canine serum.

Produces a negative reaction when analysed by ELISA or by IFAT (Indirect Fluorescent Antibody Test)

To be used as internal reference material for quality control.

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ALL SPECiES

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ALL SPECiES

ComPLEmENT FixATioN TEST

Guinea pig complement

Veronal buffer

Product Code CPLT-2x5ML

Format 2 x 5 ml vials

Product Code VB-4L

Format 4 vials (powder form). Reconstitute each vial with 1L of distilled water

Freeze-dried guinea pig complement to be used in any

complement fixation test (CFT)

Once reconstituted, the complement is stable for two

weeks at 4°C without loss of activity.

The complement system is a system of serum proteins that react with antigen-antibody complexes. If this reaction occurs on a cell surface, it will result in the formation of trans-membrane pores and therefore destruction of the cell.This characteristic is used in the complement fixation test (CFT) to detect antibodies against infectious animal diseases.

The complement fixation test requires complementary reagents that are not included in the kit. (Calcium Magnesium veronal buffer, haemolysin, sheep red blood cells, and specific antigen for CFT).

The Veronal Calcium Magnesium buffer is used to dilute

sera and reagents for the complement fixation test.

Once reconstituted, the veronal buffer is stable for 2

months at 2 – 8°C without loss of activity.

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Fully integrated disease management system Determine titres, vaccination age and baseline values for better preventative medicine for poultry.

Easily share your results with others OD results may be exported to other formats, such as Excel.

Optimize lab time and plan your testing sche-dule

The software provides you with an overview of the different tests to be run for each sample.

Flexible Collect data and edit reports from multiple work stations.

Customize your templates and reports, and incorporate your own company information and logo.

Easy access IDSoft™ may be downloaded from the IDvet website – no discs are required.

1) Download ID Soft™ from the IDvet website: www.id-vet.com/support/software.

2) Contact [email protected] to obtain the User Guide and your personal login and password.

3) Use your login and password to download kits to your repertory or to install updates as they become available.

DATA ANALySiS PRoGRAm

ID Soft™ is a fully integrated, easy-to-use software program providing convenient, flexible and comprehensive data analysis for ID Screen® ELISA kits.

ID Soft™

Features

Installing ID Soft™

TECAN Sunrise

MOLECULAR DEVICES EMax

BIOTEK

EL311

EL800

EL808

ELx800

ELx808

Reader compatibility

Microsoft Windows 2000, XP, Vista, Windows 7, Windows 8

50 MB of free hard-disk space required

Internet Connection

Minimum system requirements

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* Contain 0.03% Merthiolate as a preservative** IDvet product code or technique

Disease Status Type Species Volume Product code For use with**

RUMINANTS

AKABANE Positive Serum Sheep 1 ml MRI-AKA AKAC

BESNOITIOSIS Positive Serum Cattle 1 ml MRI-BSNT BSNTS

BHV-4 Positive Serum Catlle 0.5 ml MRI-BHV4 BHV4S

BLUETONGUE Positive Serum Cattle 1 ml MRI-BTC BTC, BTED

Positive Milk Cattle 1 ml MRI-BTMILK BTSMILK

BLV Positive Serum Cattle 1 ml MRI-BLV BLVC, BLVS

BRUCELLOSIS Positive Serum Cattle 1 ml MRI-BRU BRUS-MS, Rose Bengal, CFT, SAW

Negative Serum Cattle 1 ml MRINEG-BRU BRUS-MS, Rose Bengal, CFT

Positive Milk Cattle COMING SOON

BVD/BD/MD Positive Serum Cattle 1 ml MRI-BVD BVDOS, BVDC

Positive Milk Cattle COMING SOON

CHLAMYDOPHILA ABORTUS Positive Serum Sheep 1 ml MRI-CHLM CHLMS-MS

IBR Positive Serum Cattle 1 ml MRI-IBR IBRS, IBRGBC, IBRGEC

Positive Milk Cattle COMING SOON

INTERFERON GAMMA

(NATIVE)

Positive Plasma Cattle 0.5 ml MRI-IFNG-B IFNG

Positive Plasma Sheep 0.5 ml MRI-IFNG-O IFNG

Positive Plasma Goat 0.5 ml MRI-IFNG-C IFNG

Positive Plasma Buffalo COMING SOON

MVV / CAEV Positive Serum Sheep and Goat 0.5 ml MRI-VISNA/CAEV VISNAS/VISNAB

NEOSPORA CANINUM Positive Serum Cattle 0.5 ml MRI-NC NCS-MS

PARATUBERCULOSIS Positive Serum Cattle 1 ml MRI-PARA PARAS, PARAB

Negative Serum Cattle 2 ml MRINEG-PARA PARAS, PARAB

PPR Positive Serum Goat 1 ml MRI-PPR PPRC

Positive Antigen - 1 ml MRI-PPRAG PPRAG

Q FEVER Positive Serum Cattle 1 ml MRI-FQ FQS-MS

RIFT VALLEY FEVER IgG-positive Serum Cattle 1 ml MRI-RIFT RIFTC

IgM-positive Serum Cattle 0.5 ml MRI-RIFTIGM RIFTIGM

SCHMALLENBERG VIRUS Positive Serum Cattle 0.5 ml MRI-SBV SBVC, SBVS, SBVB

NEGATIVE BOVINE SERUM Negative Serum Cattle 0.5 ml MRINEG-BOV ID Screen® kits for Aka, BLV, Bru, BTV, IBR, Paratb, NC, SBV

AVIANIBD Positive Serum Chicken 0.5 ml MRI-IBDS IBDS

Positive Serum Chicken 0.5 ml MRI-IBDVP2 IBDVP2

IBV Positive Serum Chicken 0.25 ml MRI-IBV IBVS, IBVC

ILT Positive Serum Chicken 0.5 ml MRI-ILT ILTS

INFLUENZA A Positive Serum Chicken 1 ml MRI-FLUACA/N1 FLUACA, FLUACN1

Positive Serum Chicken 0.5 ml MRI-FLUACH7 FLUACH7

Positive and negative freeze-dried sera and milk to be used as internal reference material for quality control*

ALL SPECiES

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Disease Status Type Species Volume Product code For use with*

MYCOPLASMA

GALLISEPTICUMPositive Serum Chicken 0.5 ml MRI-MG MG/MS, MG, RSA-MG

MYCOPLASMA

MELEAGRIDISPositive Serum Turkey 1 ml MRI-MM RSA-MM

MYCOPLASMA SYNOVIAE Positive Serum Chicken 1 ml MRI-MS MG/MS, MS, RSA-MS

NDV Positive Serum Chicken 0.5 ml MRI-NDV NDVC, NDVS

SALMONELLA B & D GROUPS Positive Serum Chicken 0.5 ml MRI-SALS SALS

NEGATIVE SPF CHICKEN

SERUM

Negative Serum Chicken 0.5 ml MRINEG-BIRD ID Screen® ELISAs for avian

diseases

EQUINE

EIA Positive Serum Horse 1 ml MRI-EIA EIAED

EVA Positive Serum Horse 0.5 ml MRI-EVA EVAB

STRANGLES Positive Serum Horse 0.5 ml MRI-STREPTO STREPTOS

WEST NILE IgG-positive Serum Horse 1 ml MRI-WN WNC

IgM-positive Serum Horse 0.5 ml MRI-WNIGM WNIGM

PORCINE

AFRICAN SWINE FEVER Positive Serum Pig 0.5 ml MRI-ASF ASFB

APP Negative Serum Pig 0.5 ml MRINEG-APP ID Screen® APP ELISAs

Positive Serum Pig 0.25 ml MRI-APP-2 APPS2

Positive Serum Pig 0.25 ml MRI-APP-1-9-11 APPS1-9-11

Positive Serum Pig 0.25 ml MRI-APP-4-7 APPS4-7

Positive Serum Pig 1 ml MRI-APP-3-8 APPS3-8

Positive Serum Pig 0.25 ml MRI-APP-5 APPS5

Positive Serum Pig 1 ml MRI-APP-6 APPS6

Positive Serum Pig 0.25 ml MRI-APP-10 APPS10

Positive Serum Pig 0.25 ml MRI-APP-12 APPS12

AUJESZKY’S DISEASE Positive Serum Pig (vaccinated) 1 ml MRI-AUJGB AUJESZKYGB

Positive Serum Pig (infected) 1 ml MRI-AUJGE AUJESZKYGE

CSF Positive Serum Pig 1 ml MRI-CSFE2 CSFE2C

HEV Positive Serum Pig 1 ml MRI-HEV HEVB-MS

MYCOBACTERIUM AVIUM Positive Serum Pig 1 ml MRI-MA MA-MS

MYCOPLASMA

HYOPNEUMONIAE

Positive Serum Pig 1 ml MRI-MHYOP MHYOPS

SVD Positive Serum Pig 0.5 ml MRI-SVD SVDC

TOXOPLASMOSIS*** Positive Serum Pig 1 ml MRI-TOXO TOXOS-MS

TRICHINELLOSIS Positive Serum Pig 1 ml MRI-TRICHI TRICHIS-MS

Negative Serum Pig 1 ml MRINEG-TRICHI TRICHIS-MS

COMPANION ANIMALS

CANINE LEISHMANIASIS Positive Serum Dog 1 ml MRI-LEISH LEISHS, IFI-LEISH

Negative Serum Dog 2 ml MRINEG-LEISH LEISHS, IFI-LEISH

*** Note: The negative APP serum (MRINEG-APP) is also negative for Toxoplasmosis.

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310 rue Louis Pasteur, 34790 Grabels - FRANCE

Phone + 33 (0) 4 67 41 49 33Fax + 33 (0) 4 67 45 36 95

[email protected] www.id-vet.com

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