farm animal - AsianNet · 2014. 12. 19. · 2014 Product Catalogue. 310, rue Louis Pasteur 34790...
Transcript of farm animal - AsianNet · 2014. 12. 19. · 2014 Product Catalogue. 310, rue Louis Pasteur 34790...
With you at every step
www.id-vet.com
farm animalimmunodiagnostics
2014 Product Catalogue
310, rue Louis Pasteur34790 Grabels - FRANCE
Phone + 33 (0) 4 67 41 49 33Fax + 33 (0) 4 67 45 36 95
[email protected] www.id-vet.com
Ordering information
IDvet’s sales team and worldwide distribution network are at your service.
Please contact IDvet for technical support, or to locate the representative nearest you.
INNOVATIVE FARM ANIMAL DIAGNOSTICS
Located in Montpellier, France, IDvet develops, manufactures and sells diagnostic reagents for the detection of infectious diseases in farm animals.
With a focus on immunodiagnostics, and ELISA kits in particular, IDvet offers an extensive range of products for emerging diseases and diseases included in major prevention programs.
IDvet’s tests are used by private and public laboratories in surveillance programs and prevalence studies in over 70 countries.
HIGH-QUALITY PRODUCTS
IDvet ELISA kits, marketed under the tradename ID Screen®, contain all necessary reagents for sample testing, and are standardized to detect national and international reference sera.
The tests are robust and easy-to-use, and show innovation through simplified test protocols, ready-to-use reagents, and improved test performance.
Thanks to a highly-skilled scientific team, kit components are produced on-site to guarantee reagent quality.
Certified ISO 9001 : 2008, the company’s commitment to quality covers all activities, from Research & Development, to Production, Quality Control and Sales.
WITH YOU AT EVERY STEP
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SCIENTIFIC KNOW-HOW
IDvet strives to be at the cutting edge of veterinary diagnostics.
IDvet is the industrial partner in a number of European research programs, and collaborates closely with many international and national reference laboratories.
WITH YOU AT EVERY STEP
IDvet’s multi-lingual sales and technical team aims to provide the highest-level of customer service, offering efficient, professional technical support.
By adapting products to meet your needs, rapidly responding to your queries, providing free supplementary liquid reagents, and assisting you in kit use and result interpretation, IDvet is with you at every step.
WITH YOU AT EVERY STEP
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CoNTENTSRUMINANTS .............................................................................. 7Akabane ...............................................................................................................................8Besnoitiosis ..........................................................................................................................9BHV-4 .................................................................................................................................10Bluetongue .........................................................................................................................11Bovine Leukosis Virus ........................................................................................................14Bovine Viral Diarrhoea (BVD) / Border Disease (BD) / Mucosal Disease (MD) .................17Brucellosis ..........................................................................................................................20Chlamydophila abortus / Enzootic abortion ........................................................................24Interferon gamma ...............................................................................................................25Infectious Bovine Rhinotracheitis (IBR) ..............................................................................26Maedi Visna Virus / Caprine Arthritis Encephalitis Virus (CAEV) .......................................30Neospora caninum .............................................................................................................31Parainfluenza 3...................................................................................................................32Paratuberculosis / Johne’s Disease ...................................................................................33Peste des Petits Ruminants ...............................................................................................34Q Fever...............................................................................................................................37Rift Valley Fever .................................................................................................................38Schmallenberg virus ...........................................................................................................40Toxoplasmosis ....................................................................................................................43
EQUINE .................................................................................... 45Equine Infectious Anemia ...................................................................................................46Equine Viral Arteritis ...........................................................................................................49Influenza A ..........................................................................................................................50Strangles / Streptococcus equi ...........................................................................................52Trichinellosis .......................................................................................................................53West Nile Virus ...................................................................................................................54
AVIAN ....................................................................................... 57Infectious Bronchitis ...........................................................................................................58Infectious Bursal Disease / Gumboro Disease ...................................................................60Infectious Laryngotracheitis ................................................................................................62Influenza A ..........................................................................................................................63Avian Mycoplasmosis .........................................................................................................66Newcastle Disease .............................................................................................................70Salmonellosis .....................................................................................................................72West Nile Virus ...................................................................................................................73
SWINE ...................................................................................... 75Actinobacillus pleuropneumoniae .......................................................................................76Aujeszky’s Disease / Pseudorabies ....................................................................................78 African Swine Fever ...........................................................................................................80Brucellosis ..........................................................................................................................81Classical Swine Fever / Hog Cholera .................................................................................82Hepatitis E ..........................................................................................................................83Influenza A ..........................................................................................................................84Mycobacterium avium.........................................................................................................86Mycoplasma hyopneumoniae / Enzootic pneumoniae .......................................................87Swine Vesicular Disease ....................................................................................................89Toxoplasmosis ....................................................................................................................90Trichinellosis .......................................................................................................................91
COMPANION ANIMALS .......................................................... 93Canine Leishmaniasis ........................................................................................................94
ALL SPECIES .......................................................................... 95Complement Fixation Test ..................................................................................................96ID Soft™ Data Analysis Programme ..................................................................................97Positive and negative freeze-dried sera and milk ...............................................................98
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RumiNANTS
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Ruminants
Product code AKAC-4P AKAC-10P
Kit format 4 plates 10 plates
Reactions 384 960
Plate format 12 x 8-well strips
AkAbANE
Competitive ELISA for the detection of anti-G1 antibodies in ruminant serum and plasma.
ID Screen® Akabane Competition
Method Competitive ELISA
Species Ruminants
Specimens Serum and plasma
Coated antigen Purified virus
Conjugate Anti-G1-HRP conjugate (concentrated 10X)
Specifications
Ordering information
References
(1) Lunt, RA, White, JR, Newberry, KM, (CSIRO Livestock Industries, Geelong, Australia), Pourquier, P & Comtet, L (IDvet, Montpellier, France). Development of a commercial ELISA for the detection of serum antibody to Akabane virus. Poster presented at the 2007 WAVLD meeting, Melbourne, Australia.
(2) Comtet L. & Pourquier P. (IDvet, France). ID Screen® Schmallenberg virus Competition Multi-species: validation of a competitive ELISA for the detection of antibodies directed against the Schmallenberg virus nucleoprotein. Poster presented at the Epizone 7th Annual meeting, brussels, belgium, 2013.
High correlation with the virus neutralisation test (VNT)
Sensitive and specific, with clear separation of positive and negative results
Fast and convenient : ready-to-use components, with results in just over 2 hours.
Cost-effective for the analysis of large numbers of samples, notably in comparison with VNT.
No cross-reactions with SBV (2)
Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes colour upon addition of the sera
Associated Products
Akabane positive freeze-dried serumProduct Code MRI-AKA
Format 1 ml vial (freeze-dried)
Description Positive freeze-dried serum from an experimentally infected sheep, to be used as internal reference material for quality control.
Negative bovine freeze-dried serumProduct Code MRINEG-BOV
Format 0.5 ml vial (freeze-dried)
Description Negative freeze-dried bovine serum to be used as internal reference material for quality control.
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bESNoiTioSiS
Indirect ELISA kit for the detection of anti-Besnoitia besnoiti antibodies in bovine serum or plasma.
ID Screen® Besnoitia Indirect
Method Indirect ELISA
Species Cattle
Specimens Serum or plasma
Coated antigen Besnoitia besnoiti purified antigen
Conjugate Anti-ruminant IgG-HRP conjugate (concentrated 10X)
Specifications
Optimal specificity
High sensitivity
No sample pre-dilution required : sera are diluted directly in the ELISA plate
Easy-to-use, with results in just 90 minutes
References
Garcia-Lunar, P. et al. An Inter-Laboratory Comparative Study of Serological Tools Employed in the Diagnosis of Besnoitia besnoiti Infection in Bovines. Transboundary and Emerging Diseases, 2012. doi:10.1111/j.1865-1682.2012.01318.x
Ordering information
Product code BSNTS-2P
Kit format 2 plates
Reactions 192
Plate format 12 x 8-well strips
Associated Products
Besnoitia besnoiti positive freeze-dried serumProduct Code MRI-BSNT
Format 1 ml vial (freeze-dried)
Description Positive freeze-dried bovine serum to be used as internal reference material for quality control.
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Ruminants
Product code BHV4S-2P
Kit format 2 plates
Reactions 192
Plate format 12 x 8-well strips
bHV-4
Indirect ELISA kit for the detection of antibodies against Bovine Herpes Virus type 4 (BHV-4) in bovine serum or plasma.
ID Screen® BHV-4 Indirect
Method Indirect ELISA
Species Cattle
Specimens Serum or plasma
Coated antigen BHV-4 purified antigen
Conjugate Anti-ruminant IgG-HRP conjugate (concentrated 10X)
Specifications
Ordering information
References
Carpinschi, V, Treilles, M. (Departmental veterinary lab (LVD 50), Saint-Lo, France), Leboeuf, C. (Groupement de Défense Sanitaire, Saint-Lo, France), and Pourquier, P. (IDvet, France). Comparison of ELISA and IIF methods for the detection of BHV-4 antibodies in Bovine Serum. Poster presented at the WAVLD meeting, Madrid, 2009.
Accurate and reliable : clearly separates positive and negative results, with high sensitivity and specificity
Fast, easy-to-use, and robust, with results in 90 minutes
Associated Products
BHV-4 monoclonal antibodyProduct Code MAB-BHV4
Format 1 ml of purified monoclonal antibody, 1mg/ml.
Description Monoclonal antibody against Bovine Herpes Virus type 4 (BHV-4) for indirect immunofluorescence.
BHV-4 positive freeze-dried serumProduct Code MRI-BHV4
Format 0.5 ml vial (freeze-dried)
Description Positive freeze-dried bovine serum to be used as internal reference material for quality control.
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bLuEToNGuE
Competitive ELISA kit for the detection of anti-VP7 antibodies in serum or plasma from multiple species.
ID Screen® Bluetongue Competition
Method Competitive ELISA
Species Multiple species, including sheep, goats, cattle, buffalo, deer, and others
Specimens Serum or plasma
Coated antigen Recombinant VP7
Conjugate Anti-VP7-HRP conjugate (concentrated 10X)
Specifications
Proven specificity and sensitivity and widespread use in recent outbreaks
Detection of antibodies against all BTV serotypes, at least 5-8 days after natural infection, thanks to the use of a monoclonal antibody against the highly conserved VP7 protein
Easy-to-use : only one wash step, with results in 90 minutes
References
Vandenbussche, F., et al., Evaluation of antibody-ELISA and real-time RT-PCR for the diagnosis and profiling of bluetongue virus serotype 8 during the epidemic in Belgium in 2006., Vet. Microbiol. (2007), doi:10.1016 / j.vetmic.2007.10.029.
Ordering information
Product code BTC-4P BTC-10P
Kit format 4 plates 10 plates
Reactions 384 960
Plate format 12 x 8-well strips
Associated Products
Bluetongue positive freeze-dried serumProduct Code MRI-BTC
Format 1 ml vial (freeze-dried)
Description Positive freeze-dried bovine serum to be used as internal reference material for quality control.
Negative bovine freeze-dried serumProduct Code MRINEG-BOV
Format 0.5 ml vial (freeze-dried)
Description Negative freeze-dried bovine serum to be used as internal reference material for quality control.
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Ruminants
bLuEToNGuE
Double antigen ELISA for the early detection of anti-VP7 antibodies in serum or plasma from ruminants and other species. Efficiently detects both IgG and IgM antibodies against all BTV serotypes.
ID Screen® Bluetongue Early Detection*
Method Double antigen ELISA
Species Multiple species, including sheep, goat, cattle, buffalo, deer and others
Specimens Serum or plasma
Coated antigen Recombinant VP7
Conjugate Recombinant VP7 antigen-HRP conjugate (concentrated 10X)
Specifications
Early identifcation of infected and vaccinated animals thanks to the efficient detection of IgM antibodies and low antibody titres (IgG or IgM). Antibodies are detected 5-8 days after natural infection, and at least 7 days post- vaccination
Accurate and reliable : high specificity and sensitivity for all BTV serotypes
Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes colour upon addition of the sera
Ordering information
Product code BTED-4P
Kit format 4 plates
Reactions 384
Plate format 12 x 8-well strips
Associated Products
Bluetongue positive freeze-dried serumProduct Code MRI-BTC
Format 1 ml vial (freeze-dried)
Description Positive freeze-dried bovine serum to be used as internal reference material for quality control.
References
Oura, CAL. et al. Colostral antibody protection and interference with immunity in lambs born from sheep vaccinated with an inactivated Bluetongue serotype 8 vaccine. Vaccine (2010), doi: 10.1016/j.vaccine. 2010.01.028.
* Not available in Spain
Negative bovine freeze-dried serumProduct Code MRINEG-BOV
Format 0.5 ml vial (freeze-dried)
Description Negative freeze-dried bovine serum to be used as internal reference material for quality control.
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Product code BTSMILK-4P
Kit format 4 plates
Reactions 384
Plate format 12 x 8-well strips
bLuEToNGuE
Indirect ELISA for the detection of anti-VP7 antibodies in individual or bulk milk samples.
ID Screen® Bluetongue Milk Indirect
Method Indirect ELISA
Species Sheep, goat, cattle or buffalo
Specimens Individual or bulk milk samples.
Coated antigen Recombinant VP7
Conjugate Anti-ruminant IgG-HRP conjugate (concentrated 10X)
Specifications
Ordering information
References
Kramps, J.A, et al., Validation of a commercial ELISA for the detection of bluetongue virus (BTV)-specific antibodies in individual milk samples of Dutch dairy cows, Vet. Microbiol. (2008), doi: 10.1016/j.vetmic.2008.01.004.
The only commercial ELISA for the detection of BTV antibodies in milk
Cost-effective : the use of milk samples makes sample collection easier and more economical
Accurate, robust and reliable : high specificity and sensitivity, and excellent correlation with serum testing
Associated Products
Bluetongue positive freeze-dried milkProduct Code MRI-BTMILK
Format 1 ml vial (freeze-dried)
Description Freeze-dried positive bovine milk to be used as internal reference material for quality control.
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Ruminants
Product code BLVS-4P BLVS-10P
Kit format 4 plates 10 plates
Reactions 384 960
Plate format 12 x 8-well strips
boViNE LEukoSiS ViRuS
Indirect ELISA for the detection of anti-BLV antibodies in bovine or buffalo sera and plasma (individual and pools of ten samples).
ID Screen® BLV Indirect
Method Indirect ELISA
Species Cattle or buffalo
Specimens Serum and plasma (individual or pools of up to 10 samples)
Coated antigen Purified virus
Conjugate Anti-ruminant IgG-HRP Conjugate (concentrated 10x)
Specifications
Ordering information
References
(1) OIE Terrestrial Manual 2012; 2.4.11; Enzootic Bovine Leukosis.
(2) European directive CEE 64/432
Excellent specificity and sensitivity
Calibrated to detect the E05 serum standard1
Straightforward, versatile test, with short or overnight incubations for individual or pooled serum and plasma.
Negative bovine freeze-dried serumProduct Code MRINEG-BOV
Format 0.5 ml vial (freeze-dried)
Description Negative freeze-dried bovine serum to be used as internal reference material for quality control.
Associated Products
BLV positive freeze-dried serumProduct Code MRI-BLV
Format 1 ml vial (freeze-dried)
Description Positive freeze-dried bovine serum to be used as internal reference material for quality control
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Product code BLVC-4P BLVC-10P
Kit format 4 plates 10 plates
Reactions 384 960
Plate format 12 x 8-well strips
boViNE LEukoSiS ViRuS
Competitive ELISA kit for the detection of antibodies against the gp51 envelope protein of the Enzootic Bovine Leukosis Virus.
ID Screen® BLV Competition
Method Competitive ELISA
Species Cattle or buffalo
Specimens Serum or plasma (individual or pools of up to 10 samples)
Coated antigen Purified virus
Conjugate Anti-gp51-HRP Conjugate (concentrated 10x)
Specifications
Ordering information
References
(1) OIE Terrestrial Manual 2012; 2.4.11; Enzootic Bovine Leukosis.
(2) European directive CEE 64/432
Excellent specificity and sensitivity
Calibrated to detect the E05 serum standard1
Straightforward, versatile test, with short or overnight incubations for individual or pooled serum or plasma.
Negative bovine freeze-dried serumProduct Code MRINEG-BOV
Format 0.5 ml vial (freeze-dried)
Description Negative freeze-dried bovine serum to be used as internal reference material for quality control.
Associated Products
BLV positive freeze-dried serumProduct Code MRI-BLV
Format 1 ml vial (freeze-dried)
Description Positive freeze-dried bovine serum to be used as internal reference material for quality control
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Ruminants
Product code BLV-AGID BLV-AGID-NOGEL
Kit format Includes agar gel Agar gel not included
Reactions 240 240
boViNE LEukoSiS ViRuS
Agar gel immunodiffusion (AGID) test for the detection of BLV anti-gP51 antibodies in bovine serum.
IDvet BLV AGID
Method AGID
Species Bovine
Specimens Serum
Specifications
Ordering information
References
(1) OIE Terrestrial Manual 2012; 2.4.11; Enzootic Bovine Leukosis.
(2) European directive CEE 64/432
Bright, distinct precipitation lines facilitate test interpretation
Standardised against the European Standard E05
Associated Products
BLV AGID CUTTERProduct Code BLVCUTTER
Format As per the European Commission Decision of 15 December 2009 amending Annex D:• Diameter of central well: 4 mm• Diameter of peripheral wells: 6 mm• Distance between central and peripheral wells: 3 mm
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References
(1) OIE Terrestrial Manual 2008; 2.4.8; Bovine Viral Diarrhoea
boViNE ViRAL DiARRHoEA (bVD) / muCoSAL DiSEASE (mD)/ boRDER DiSEASE (bD)
Sandwich ELISA for the detection of the p80-125 protein of Bovine Viral Diarrhoea, Musocal Disease and Border Disease, in leukocyte fractions or ear notch samples from ruminants and all susceptible species.
ID Screen® BVD p80 Antigen
Method Sandwich ELISA
Species Bovine, ovine, caprine, and all susceptible species
Specimens Leukocyte fractions, ear notch samples
Coated antibody Anti-p80/125 monoclonal antibody
Conjugate Anti-p80/125-HRP conjugate (concentrated 10X)
Specifications
Ordering information
Product code BVDAG-4P BVDAG-10P
Kit format 4 plates 10 plates
Reactions 384 960
Plate format 12 x 8-well strip
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Ruminants
References
(1) OIE Terrestrial Manual 2008; 2.4.8; Bovine Viral Diarrhoea
boViNE ViRAL DiARRHoEA (bVD) / muCoSAL DiSEASE (mD)/ boRDER DiSEASE (bD)
One-stepTM ELISA for the detection of Bovine Viral Diarrhoea / Mucosal Disease / Border Disease anti-p80-125 (anti-NSP2-3) antibodies in serum and plasma from cattle, sheep, goats and other susceptible species.
ID Screen® BVD p80 Antibody One-Step™
Method One-StepTM ELISA
Species Cattle, sheep, goats and other susceptible species.
Specimens Serum or plasma
Coated antibody Anti-p80/125 Mab
Conjugate p80/125 antigen-HRP conjugate (concentrated 10X)
Specifications
Fast and easy-to-use : only one incubation step (co-incubation of serum and conjugate), with results in under 90 minutes
Accurate and reliable : high specificity and sensitivity
Ordering information
Product code BVDOS-4P
Kit format 4 plates
Reactions 384
Plate format 12 x 8-well strips
Associated Products
BVD/MD/BD positive freeze-dried serumProduct Code MRI-BVD
Format 1 ml vial (freeze-dried)
Description Antibody-positive freeze-dried bovine serum to be used as internal reference material for quality control.
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References
(1) OIE Terrestrial Manual 2008; 2.4.8; Bovine Viral Diarrhoea
boViNE ViRAL DiARRHoEA (bVD) / muCoSAL DiSEASE (mD)/ boRDER DiSEASE (bD)
Competitive ELISA for the detection of Bovine Viral Diarrhoea / Mucosal Disease / Border Disease anti-p80-125 (anti-NSP2-3) antibodies in serum, plasma and milk from cattle, sheep, goats and other susceptible species.
ID Screen® BVD p80 Antibody Competition
Fast and easy-to-use: ready-to-use conjugate and results in 90 minutes
Flexible : short and overnight protocols for serum, plasma or milk
Applicable to multiple species, including cattle, sheep, and goats
Accurate and reliable: high specificity and sensitivity
Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes colour upon addition of the sera
Method Competitive ELISA
Species Cattle, sheep, goats and other susceptible species.
Specimens Individual serum and plasma samplesMilk (individual samples or pools of up to 50)
Incubations Short and overnight incubations for serum, plasma and milk
Coated antigen p80/125 purified antigen
Conjugate Ready-to-use anti-p80/125-HRP monoclonal antibody
Specifications
Ordering information
Product code BVDC-4P BVDC-10P
Kit format 4 plates 10 plates
Reactions 384 960
Plate format 12 x 8-well strip
Associated Products
BVD/MD/BD positive freeze-dried serumProduct Code MRI-BVD
Format 1 ml vial (freeze-dried)
Description Antibody-positive freeze-dried bovine serum to be used as internal reference material for quality control.
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Ruminants
Product code AG-BRU
Kit format 100 ml
Reactions ~ 40000 reactions
bRuCELLoSiS
Brucella abortus antigen for the Complement Fixation Test.
Brucellosis Antigen for the Complement Fixation Test
Ordering information
References
(1) OIE Terrestrial Manual 2009; 2.4.3; Bovine brucellosis.
Suspension of Brucella abortus biovar 1 Weybridge strain No 99, inactivated by heat and phenol.
Calibrated to give 50% haemolysis with the second international standard serum (OIESS) diluted1/200
Associated Products
Brucellosis positive freeze-dried serumProduct Code MRI-BRU
Format 1 ml vial (freeze-dried)
Description Freeze-dried highly positive bovine serum to be used as internal reference material for quality control.
Brucellosis negative freeze-dried serumProduct Code MRINEG-BRU
Format 1 ml vial (freeze-dried)
Description Freeze-dried negative bovine serum to be used as internal reference material for quality control.
Guinea pig complementProduct Code CPLT-2x5ML
Format 2 x 5 ml vials
Description Freeze-dried guinea pig complement to be used in any complement fixation test (CFT).
Veronal bufferProduct Code VB-4L
Format 4 vials (powder form). Reconstitute each vial with 1L of distilled water
Description The Veronal Calcium Magnesium buffer is used to dilute sera and reagents for the complement fixation test.
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References
(1) OIE Terrestrial Manual 2009; 2.4.3; Bovine brucellosis.
(2) Annex C of the European directive CEE 64/432
bRuCELLoSiS
Rapid slide agglutination antigen for the detection of anti-Brucella abortus, melitensis, or suis antibodies in bovine, ovine, caprine, and porcine species.
Rose Bengal (Rapid slide agglutination antigen)
Suspension of Brucella abortus biovar 1 Weybridge strain No 99, inactivated by heat and phenol and coloured with rose bengal stain in an acidified buffer.
Calibrated on the OIEISS International Brucella standard serum and the national secondary serum to be positive against a 1/45 dilution and negative against a 1/55 dilution, as per the European directive CEE 64/432 and OIE requirements.
Ordering information
Product code RSA-RB
Kit format 10 ml
Reactions 330 reactions (30 µl per test)
Associated Products
Brucellosis positive freeze-dried serumProduct Code MRI-BRU
Format 1 ml vial (freeze-dried)
Description Freeze-dried highly positive bovine serum to be used as internal reference material for quality control.Positive when tested with Rose Bengal.
Brucellosis negative freeze-dried serumProduct Code MRINEG-BRU
Format 1 ml vial (freeze-dried)
Description Freeze-dried negative bovine serum to be used as internal reference material for quality control.Negative when tested with Rose Bengal.
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Ruminants
Product code BRUS-MS-4P BRUS-MS-10P
Kit format 4 plates 10 plates
Reactions 384 960
Plate format 12 x 8-well strips
bRuCELLoSiS
Indirect ELISA for the detection of antibodies against Brucella abortus, melitensis or suis in bovine, ovine, caprine and porcine serum and plasma (individual samples or pools of up to 10).
ID Screen® Brucellosis Serum Indirect Multi-species
Method Indirect ELISA
Species Bovine, ovine, caprine and porcine. Please contact IDvet for use in other species.
Specimens Individual serum or plasma samples, or pools of up to 10 samples
Coated antigen Purified Brucella LPS
Conjugate Anti-multi-species-IgG-HRP conjugate (concentrated 10X)
Specifications
Ordering information
References
(1) OIE Terrestrial Manual 2009; 2.4.3; Bovine brucellosis.
(2) Annex C of the European directive CEE 64/432
Calibrated according to OIE specifications and Annex C of the European directive CEE 64/432 to correctly detect the OIEELISASPISS standard serum.
Straightforward, versatile test, with short and overnight incubations for individual serum or plasma samples, or pools of up to 10 samples.
Multi-species test for ruminants and swine
Associated Products
Brucellosis positive freeze-dried serumProduct Code MRI-BRU
Format 1 ml vial (freeze-dried)
Description Freeze-dried highly positive bovine serum to be used as internal reference material for quality control.
Brucellosis negative freeze-dried serumProduct Code MRINEG-BRU
Format 1 ml vial (freeze-dried)
Description Freeze-dried negative bovine serum to be used as internal reference material for quality control.
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References
(1) OIE Terrestrial Manual 2009; 2.4.3; Bovine brucellosis.
(2) Annex C European directive CEE 64/432
bRuCELLoSiS
Indirect ELISA for the detection of antibodies against Brucella abortus or melitensis in bovine, ovine and caprine milk. The test may be used on individual or bulk milks*.The assessment of the diagnostic performance of this ELISA kit on field samples has shown that positive bovine milk samples may be detected in pools of up to 250 samples.
* Note: The analysis of milk from small ruminants and the analysis of individual milks are not official EU tests.
ID Screen® Brucellosis Milk Indirect
Calibrated according to the Annex C European directive CEE 64/432 and OIE requirements
Straightforward test for individual or pooled milks.
Ordering information
Product code BRUMILK-4P
Kit format 4 plates
Reactions 384
Plate format 12 x 8-well strips
Method Indirect ELISA
Species Ruminants
Specimens Bovine (cattle and buffalo), ovine and caprine milk (individual or up to 250 pooled samples).
Coated antigen Purified Brucella LPS
Conjugate Anti-ruminant IgG-HRP conjugate (concentrated 10X)
Specifications
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Ruminants
References
(1) Horigan, MW et al. (Veterinary Laboratories Agency, UK). Poster : Chlamydophila abortus – An Evaluation of Three Commercial ELISAs. Presented at the WAVLD Conference in madrid, Spain, 2009.
(2) Marques, PX et al. (University College, Dublin, Ireland). Poster: A comparison of three serological methods for the identification of pregnant ewes infected with Chlamydophila abortus. 2008.
(3) Pourquier, P. (IDvet, France), Rodalakis, A and Mohamad, KY (INRA, Nouzilly, France). Preliminary validation of a new commercial ELISA kit for the detection of antibodies directed against C. abortus. Presented at the WAVLD Conference, 2007.
CHLAmyDoPHiLA AboRTuS / ENzooTiC AboRTioN
This indirect multi-species ELISA detects anti-MOMP (Major Outer Membrane Protein) antibodies in ruminant serum and plasma. Please contact IDvet for use on other species.
ID Screen® Chlamydophila abortus Indirect Multi-species
Method Indirect ELISA
Species Ruminants and other species
Specimens Serum and plasma
Coated antigen MOMP peptide
Conjugate Anti-multi-species IgG–HRP conjugate (concentrated 10X)
Specifications
High sensitivity and superior specificity with respect to C. pecorum thanks to the use of peptide antigens
Applicable to multiple species, including sheep, goats and cattle. Please consult IDvet for use with other species
Ordering information
Product code CHLMS-MS-2P
Kit format 2 plates
Reactions 192
Plate format 12 x 8-well strips
Associated Products
Chlamydophila abortus positive freeze-dried serum
Product Code MRI-CHLM
Format 1 ml vial (freeze-dried)
Description Positive freeze-dried ovine serum to be used as internal reference material for quality control.
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Product code IFNG-2P IFNG-4P IFNG-10P
Kit format 2 plates 4 plates 10 plates
Reactions 192 384 960
Plate format 12 x 8-well strips
iNTERFERoN GAmmA
Sandwich ELISA for the detection of ovine, bovine and caprine interferon gamma (IFN-g ) in activated plasma or in culture supernatant.
ID Screen® Ruminant IFN-g
Method Sandwich ELISA
Species Bovine, ovine and caprine
Specimens Activated plasma or culture supernatant
Coated antibody Anti-ruminant IFN-g monoclonal antibody
Conjugate Anti-ruminant IFN-g HRP Mab conjugate
Specifications
Ordering information
References
Comtet, L., Pourquier, P. (IDvet). ID Screen® Interferon gamma ELISA: improvement of analytical sensitivity and introduction of a standard reference control to improve result interpretation. Poster presented at the 2012 International Colloquium on Paratuberculosis, Sydney, Australia.
Standardized results thanks to a freeze-dried positive reference control used to calculate S/P ratios for each sample.
Flexible: the same kit may be used with both native antigens (standard protocol) and recombinant / peptide antigens (alternative protocol)
Practical: the standard protocol uses only 10ul per sample, meaning that less sample volume is required for activation
Associated Products
Interferon gamma positive activated freeze-dried plasmasSpecies Bovine Ovine Caprine Buffalo
Product Code MRI-IFNG-B MRI-IFNG-O MRI-IFNG-C MRI-IFNG-BF
Format 0.5 ml vial (freeze-dried)
Reagents for in vitro cell activationProduct Code PPDPACK
Format Reagents for 5000 reactions
Description In vitro cell activation pack for 5000 reactions containing avian and bovine tuberculin PPD and mitogen (pokeweed). Mitogen is used as a positive activation control
26
Ruminants
Product code IBRS-4P IBRS-10P
Kit format 4 plates 10 plates
Reactions 384 960
Plate format 12 x 8-well strips
iNFECTiouS boViNE RHiNoTRACHEiTiS (ibR)
Indirect ELISA for the detection of anti-BHV-1 (Bovine Herpesvirus type 1) antibodies in bovine serum and plasma.
ID Screen® IBR Indirect
Method Indirect ELISA
Species Cattle and buffalo
Specimens Serum and plasma
Coated antigen BHV-1 purified lysat
Conjugate Anti-ruminant IgG-HRP conjugate (concentrated 10X)
Specifications
Ordering information
References
(1) OIE Terrestrial Manual 2010; 2.4.13; Infectious bovine rhinotracheitis/infectious pustular vulvovaginitis.
Calibrated to detect the EU2 (weak-positive) standard serum 1
Straightforward and rapid, with short and overnight incubations and results in 90 minutes.
Associated Products
IBR positive freeze-dried serumProduct Code MRI-IBR
Format 1 ml vial (freeze-dried)
Description Freeze-dried positive bovine serum to be used as internal reference material for quality control.
Negative bovine freeze-dried serumProduct Code MRINEG-BOV
Format 0.5 ml vial (freeze-dried)
Description Negative freeze-dried bovine serum to be used as internal reference material for quality control.
27
References
(1) OIE Terrestrial Manual 2010; 2.4.13; Infectious bovine rhinotracheitis/infectious pustular vulvovaginitis.
iNFECTiouS boViNE RHiNoTRACHEiTiS (ibR)
Indirect ELISA for the detection of anti-BHV-1 (Bovine Herpesvirus type 1) antibodies in bovine bulk milk.
ID Screen® IBR Milk Indirect
Method Indirect ELISA
Species Cattle and buffalo
Specimens Bulk milk
Coated antigen BHV-1 purified lysat
Conjugate Anti-ruminant IgG – HRP (concentrated 10X)
Specifications
Cost-effective: the use of milk samples makes sample collection easier and more economical.
Accurate, robust and reliable : high specificity and sensitivity, and excellent correlation with serumtesting
Rapid and easy-to-use
Short and overnight protocols
Ordering information
Product code IBRMILK-4P IBRMILK-10P
Kit format 4 plates 10 plates
Reactions 384 960
Plate format 12 x 8-well strips
28
Ruminants
References
(1) OIE Terrestrial Manual 2010; 2.4.13; Infectious bovine rhinotracheitis/infectious pustular vulvovaginitis.
iNFECTiouS boViNE RHiNoTRACHEiTiS (ibR)
Competitive ELISA for the detection of anti-gB antibodies in serum and plasma.
ID Screen® IBR gB Competition
Method Competitive ELISA
Species Cattle and buffalo
Specimens Serum and plasma
Incubations Short and overnight
Coated antigen BHV-1 purified lysat
Conjugate Ready-to-use anti-gB-HRP conjugate
Specifications
Calibrated to detect the EU2 (weak-positive) standard serum1.
Flexible test: short and overnight protocols.
Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes colour upon addition of the sera
The stop solution no longer contains H2SO4, meaning that it is less corrosive
Ordering information
Product code IBRGBC-4P IBRGBC-10P
Kit format 4 plates 10 plates
Reactions 384 960
Plate format 12 x 8-well strips
Associated Products
IBR positive freeze-dried serumProduct Code MRI-IBR
Format 1 ml vial (freeze-dried)
Description Freeze-dried positive bovine serum to be used as internal reference material for quality control.
Negative bovine freeze-dried serumProduct Code MRINEG-BOV
Format 0.5 ml vial (freeze-dried)
Description Negative freeze-dried bovine serum to be used as internal reference material for quality control.
29
References
(1) OIE Terrestrial Manual 2010; 2.4.13; Infectious bovine rhinotracheitis/infectious pustular vulvovaginitis.
iNFECTiouS boViNE RHiNoTRACHEiTiS (ibR)
Competitive ELISA for the detection of anti-BHV-1 gE antibodies in bovine serum, plasma and milk (individual, bulk or condensed milk samples).Detects naturally-infected animals in a vaccinated population provided that animals are vaccinated with BHV-1 gE-deleted vaccines.
ID Screen® IBR gE Competition
Method Competitive ELISA
Species Bovine
Specimens Serum, plasma and milk (individual, bulk or condensed milk samples)
Coated antigen BHV-1 purified lysat
Conjugate Anti-gE-HRP conjugate (concentrated 10X)
Specifications
May be used as a DIVA test (Differentiation of Infected and Vaccinated Animals)
Calibrated to detect the EU2 (weak-positive) standard serum1.
Ordering information
Product code IBRGEC-4P IBRGEC-10P
Kit format 4 plates 10 plates
Reactions 384 960
Plate format 12 x 8-well strips
Associated Products
IBR positive freeze-dried serumProduct Code MRI-IBR
Format 1 ml vial (freeze-dried)
Description Freeze-dried positive bovine serum to be used as internal reference material for quality control.
Negative bovine freeze-dried serumProduct Code MRINEG-BOV
Format 0.5 ml vial (freeze-dried)
Description Negative freeze-dried bovine serum to be used as internal reference material for quality control.
30
Ruminants
References
(1) Comtet, L. (IDvet), Feliziani, F. (CEREL – IZS Umbria e Marche, Italy), Lesceu, S. (IDvet). Validation of the ID Screen® Maedi Visna Indirect ELISA: specificity on BTV-8 vaccinated sheep and detection of seroconversion. Poster presented at the 2010 EAVLD meeting, Lelystad, Holland.
mAEDi ViSNA ViRuS / CAPRiNE ARTHRiTiS ENCEPHALiTiS ViRuS (CAEV)
Indirect ELISA kit for the detection of anti-Maedi Visna Virus (MVV) and anti-CAEV antibodies in ovine and caprine serum. The use of a mix of peptide antigens results in superior test performance (sensitivity, specificity, and differentiation between positive and negative results).
ID Screen® MVV / CAEV Indirect
Method Indirect ELISA
Species Sheep and goats
Specimens Serum or plasma
Coated antigen Mix of peptides from the GAG, TM and ENV genes.
Conjugate Anti-ruminant IgG-HRP conjugate (concentrated 10X)
Specifications
Reliable and accurate : clearly separates positive and negative results, with high sensitivity and specificity
No non-specific reactions due to BTV-vaccination, thanks to the use of peptide antigens. Other ELISAs based on recombinant or purified viral antigens have given non-specific reactions in BTV-vaccinated populations 1
Easy-to-use, with results in 90 minutes
Ordering information
Associated Products
MVV / CAEV positive freeze-dried serumProduct Code MRI-VISNA / CAEV
Format 0.5 ml vial (freeze-dried)
Description Freeze-dried positive ovine and caprine serum pool to be used as internal reference material for quality control.
Confirmation format
Product code VISNAB-4P
Kit format 4 plates (bi-well)
Reactions 192
Plate format 6 x 16-well strips
Screening format
Product code VISNAS-4P
Kit format 4 plates
Reactions 384
Plate format 12 x 8-well strips
* Each sample is tested in duplicate in a coated well (even columns) and in an adjacent non-coated well (odd columns). Results are cal-culated with respect to the corrected sample oD value (even well oD – odd well oD).
31
Product code NCS-MS-2P NCS-MS-5P
Kit format 2 plates 5 plates
Reactions 192 480
Plate format 12 x 8-well strips
NEoSPoRA CANiNum
Indirect multi-species ELISA kit for the detection of anti-Neospora caninum antibodies in serum or milk.
ID Screen® Neospora caninum Indirect Multi-species
Method Indirect ELISA
Species Multiple species, including ruminants and dogs.
Specimens Serum, plasma or milk
Coated antigen Purified Neospora caninum extract
Conjugate Anti-multi-species IgG –HRP conjugate (concen-trated 10X)
Specifications
Ordering information
Cost-effective method for disease monitoring, with high specificity and sensitivity
Fast and convenient protocol with results in 60 minutes and protocols for both serum and milk samples
Applicable to multiple species, including ruminants and dogs.
Associated Products
N. caninum positive freeze-dried serumProduct Code MRI-NC
Format 0.5 ml vial (freeze-dried)
Description Freeze-dried positive bovine serum to be used as internal reference material for quality control
Negative bovine freeze-dried serumProduct Code MRINEG-BOV
Format 0.5 ml vial (freeze-dried)
Description Negative freeze-dried bovine serum to be used as internal reference material for quality control.
References
Alvarez-García, G., et al., Serological diagnosis of bovine neosporosis: A comparative study of commercially available ELISA tests. Vet. Parasitol. (2013), http://dx.doi.org/10.1016/j.vetpar.2013.07.033
32
Ruminants
PARAiNFLuENzA 3
PI-3 monoclonal antibody
Product code MAB-PI3
Description Monoclonal antibody against Parainfluenza-3 virus for indirect immunofluorescence
Format 1 ml of purified monoclonal antibody, 1mg/ml
33
PARATubERCuLoSiS / JoHNE’S DiSEASE
Indirect absorbed(3) ELISA for the detection of anti-Mycobacterium avium subsp. paratuberculosis (Map) antibodies in ruminant serum, plasma and milk.
ID Screen® Paratuberculosis Indirect
Method Indirect absorbed ELISA
Species Ovine, caprine, bovine (cattle or buffalo)
Specimens Serum, plasma or bovine milk
Coated antigen Purified MAP extract
Conjugate Anti-ruminant-HRP conjugate (concentrated 10X)
Incubations Short or overnight
Specifications
Ideal for herd certification and disease eradication programs thanks to high sensitivity, specificity and clear separation of positive and negative results
Automatable and rapid : results in 90 minutes.
Screening and confirmation formats available
Short or overnight incubations
Screening format
Product code PARAS-4P PARAS-10P
Kit format 4 plates 10 plates
Reactions 384 960
Plate format 12 x 8-well strips
Confirmation format*
Product code PARAB-4P
Kit format 4 plates (bi-well)
Reactions 192
Plate format 6 x 16-well strips
Associated Products
Mycobactine JProduct Code MYCO
Format 2 mg vial (freeze-dried)
Description Ferric Mycobactin J is an iron-chelated substance used as a growth factor for the isolation of mycobactin-dependent Mycobacterium.
Paratuberculosis positive freeze-dried serumProduct Code MRI-PARA
Format 1 ml vial (freeze-dried)
Description Freeze-dried positive bovine serum to be used as internal reference material for quality control.
Ordering information
References
(1) Köhler, Heike et al. Evaluation of five ELISA test kits for the measurement of antibodies against Mycobacterium avium subspecies paratuberculosis in bovine serum. Berl. Münch. Tierärztl. Wochenschr. 121, Heft 5/6, 203-210 (2008).
(2) Fry, M., Kruze, J., Collins, M., Evaluation of four commercial enzyme-linked immunosorbent assays for the diagnosis of bovine paratuberculosis in Chilean dairy herds. J Vet Diagn Invest 20:329-332 (2008).
(3) OIE recommendations: Manual of Recommended Diagnostic Techniques and Requirements volume III -Paratuberculosis 5B/009.
* Each sample is tested in a coated well (even columns) and in an adjacent non-coated well (odd columns). Results are calculated with respect to the corrected sample oD (even well oD – odd well oD).
Paratuberculosis negative freeze-dried serumProduct Code MRINEG-PARA
Format 2 ml vial (freeze-dried)
Description Freeze-dried negative bovine serum to be used as internal reference material for quality control.
34
Ruminants
Product code PPRC-4P PPRC-10P
Kit format 4 plates 10 plates
Reactions 384 960
Plate format 12 x 8-well strips
PESTE DES PETiTS RumiNANTS
Competitive ELISA kit for the detection of anti-PPRV nucleoprotein (NP) antibodies in sheep and goat serum or plasma. Please contact IDvet for use on other species.
The only commercial ELISA approved by the FAO / OIE CIRAD laboratory, and based on the CIRAD biological components (antigen and conjugate).
This test does not contain any virus particles.
ID Screen® PPR Competition
Method Competitive ELISA
Species Multiple species, including sheep, goats and others
Specimens Serum or plasma
Coated antigen Recombinant PPRV nucleoprotein (NP)
Conjugate Anti-NP-HRP conjugate (concentrated 10X)
Specifications
Ordering information
References
Libeau, G., Préhaud, C., Lancelot, R., Colas, F., Guerre, L., Bishop, D.H., Diallo, A. Development of a competitive ELISA for detecting antibodies to the Peste des Petits Ruminants virus using a recombinant nucleoprotein. Res Vet Sci. 1995 Jan;58(1):50-5.
Convenient format: The only commercial ELISA to supply ready-to-use, coated plates, and all other components in liquid format. Plates are supplied in strips
Straightforward and rapid protocol : each sample is deposited only once (screening format), and results are obtained in 90 minutes
High specificity and sensitivity
Associated Products
PPR positive freeze-dried serumProduct Code MRI-PPR
Format 1 ml vial (freeze-dried)
Description Freeze-dried positive caprine serum to be used as internal reference material for quality control.
35
Product code PPRAG-1P PPRAG-2P
Kit format 1 plate 2 plates
Reactions 96 192
Plate format 12 x 8-well strips
PESTE DES PETiTS RumiNANTS
Sandwich ELISA for the detection of Peste des Petits Ruminants virus in live animals or for post-mortem diagnosis.
Based on reagents developed at a FAO/OIE PPRV reference laboratory (CIRAD, Montpellier, France). IDvet contributed to the improvement of this test by optimizing test performance and component stability.
This test does not contain any virus particles.
ID Screen® PPR Antigen Capture
Method Sandwich ELISA
Species Ovine and caprine
Specimens Variety of sample types, including oral, nasal or rectal swabs as well as tears, tissue samples, gum debris or PBMC
Coated antibody Anti-PPRV NP antibody
Conjugate Anti-PPRV NP Mab–HRP conjugate (concentrated 10X)
Specifications
Ordering information
References
(1) O. Kwiatek, Y.H. Ali, I.K. Saeed, A.I. Khalafalla, O.I. Mohamed, A.A. Obeida, M.B. Abdelrahman, H.M. Osman, K.M. Taha, Z. Abbas, M. El Harrak, Y. Lhor, A. Diallo, R. Lancelot, E. Albina, G. Libeau. Asian lineage of peste des petits ruminants virus, Africa. Emerg. Infect. Dis., 17 (2011), pp. 1223–1231
(2) Recognizing peste des petits ruminants - A field manual, FAO, section: diagnosis of PPR. URL link: http://www.fao.org/docrep/003/x1703e/x1703e00.htm#Diagnosis of PPR
(3) Libeau, G., Diallo, A., Colas, F., Guerre, L., 1994. Rapid differential diagnosis of rinderpest and peste des petits ruminants using an immunocapture ELISA. Vet.Rec. 19, 300–304
(4) Abubakar, M., et al. Comparison of antigen detection methods of peste des petits ruminants virus in clinical samples of small ruminants. Bulgarian Journal of Veterinary Medicine (2011), 14, No 2, 103-108
(5) Couacy-Hymann, E., et al. The early detection of peste-des-petits-ruminants (PPR) virus antigens and nucleic acid from experimentally infected goats using RT-PCR and immunocapture ELISA techniques. Research in Veterinary Science, Volume 87, Issue 2, October 2009, 332335
(6) Muhammad M., et al. (2012). Serodiagnosis of Peste des Petits Ruminants Virus, Serological Diagnosis of Certain Human, Animal and Plant Diseases, Dr. Moslih Al-Moslih (Ed.), ISBN: 978-953-51-0370-7, InTech, Available from: http://www.intechopen.com/books/serologicaldiagnosis-of-certain-human-animal-and-plant-diseases/serodiagnosis-of-peste-des-petits-ruminants-virus
(7) Comtet, L., Pourquier, P. (IDvet, France), Kwiatek, O., Libeau, G. (CIRAD, France). Validation of the ID Screen® Peste des Petits Ruminants Antigen Capture ELISA. Poster presented at the Epizone 7th Annual meeting, brussels, belgium, 2013.
Equivalent performance to RT-PCR 7
Improved sensitivity for samples with weak or strong PPRV titres
Flexible test for use with a wide variety of matrices, including oral, nasal or rectal swabs as well as tears, tissue samples, gum debris or PbmC.
Convenient and easy-to-use: microplates are supplied coated in strips of 8 wells.
Straightforward and rapid protocol, with results in 90 minutes
Associated Products
Freeze-dried PPR AntigenProduct Code MRI-PPRAG
Format 1 ml vial (freeze-dried)
Description Freeze-dried inactivated PPR antigen to be used as internal reference material for quality control.
36
Ruminants
37
Q FEVER
Indirect multi-species ELISA for the detection of anti-Coxiella burnetii antibodies in serum and plasma or in individual or bulk milk samples from multiple species.
ID Screen® Q Fever Indirect Multi-species
Method Indirect ELISA
Species Multiple species, including cattle, sheep, goatsand others
Specimens Serum, plasma or milk
Coated antigen Inactivated Coxiella burnetii antigen from a bovine isolate
Conjugate Anti-multi-species IgG-HRP conjugate (concentrated 10X)
Specifications
Applicable to multiple species, including cattle, sheep and goats. Please contact iDvet for use in other species.
Straightforward, easy-to-use, with results in 90 minutes
Ordering information
Product code FQS-MS-2P
Kit format 2 plates
Reactions 192
Plate format 12 x 8-well strips
Associated Products
Q Fever positive freeze-dried serumProduct Code MRI-FQ
Format 1 ml vial (freeze-dried)
Description Freeze-dried positive bovine serum to be used as internal reference material for quality control
38
Ruminants
Product code RIFTC-4P RIFTC-10P
Kit format 4 plates 10 plates
Reactions 384 960
Plate format 12 x 8-well strips
RiFT VALLEy FEVER
Competitive ELISA kit for the detection of anti-Rift Valley Fever (RVFV) nucleoprotein antibodies in ruminant serum or plasma.
This test does not contain any virus particles.
ID Screen® Rift Valley Fever Competition Multi-species
Method Competitive ELISA
Species Multiple species, including ruminants, camels, horses, dogs and others
Specimens Serum or plasma
Coated antigen RVF Virus recombinant nucleoprotein (NP)
Conjugate Anti-NP-HRP conjugate (concentrated 10X)
Specifications
Ordering information
References
(1) Comtet, L. et Pourquier, P. (IDvet), Marié, J-L. & Davoust, B. (French Defence Medical Service, Working group of animal epidemiology, Marseille, France), Cêtre-Sossah, C. (CIRAD, Montpellier, France). Preliminary validation of the ID Screen® Rift Valley Fever Competition Multi-species ELISA. Poster presented at the 2010 EAVLD meeting, Lelystad, The Netherlands.
(2) Ahmed B. et al. Unexpected Rift Valley Fever Outbreak, Northern Mauritania. Emerging Infectious Diseases. Vol. 17, No. 10. October 2011.
(3) Kortekaas, J. et al. European ring trial to evaluate ELISAs for the diagnosis of infection with Rift Valley fever virus. Journal of Virological Methods. September 2012.
(4) Antonis, A.F.G. et al. Vertical Transmission of Rift Valley Fever Virus Without Detectable Maternal Viremia. Vector-borne and zoonotic diseases. Vol. 13, No. X. May 2013.
Excellent analytical sensitivity, sensitivity and specificity, as proven in recent outbreaks
Multi-species test : may be used on ruminants, horses, dogs and other species
Convenient and rapid format: ready-to-use, coated plates in strips, and all other components in liquid format. Results in less than 2 hours
Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes colour upon addition of the sera
Associated Products
Rift Valley Fever IgG-positive freeze-dried serumProduct Code MRI-RIFT
Format 1 ml vial (freeze-dried)
Description Freeze-dried positive bovine serum to be used as internal reference material for quality control
39
RiFT VALLEy FEVER
IgM Antibody Capture (IMAC) ELISA for the detection of anti-nucleoprotein (NP) IgM antibodies in ruminant serum and plasma. The detection of anti-IgM antibodies indicates recent infection.
This test does not contain any virus particles.
ID Screen® Rift Valley Fever IgM Capture
Specifications
Accurate and reliable : excellent specificity and analytical and diagnostic sensitivity
Convenient format: Ready-to-use, coated plates in strips, and all other components in liquid format.
Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes colour upon addition of the sera
Product code RIFTIGM-4P RIFTIGM-10P
Kit format 4 plates (biwell format*)
10 plates (biwell format*)
Reactions 192 480
Plate format 6 x 16 well strips
Ordering information
Method IMAC (IgM Antibody Capture) ELISA
Species Ruminants
Specimens Serum or plasma
Coated antibody Anti-bovine-ovine-caprine IgM antibody
Antigen RVFV recombinant nucleoprotein (freeze-dried)
Conjugate Anti-NP-HRP conjugate (concentrated 10X)
Protocol* (1) Sample Incubation(2) Three washes(3) RVF nucleoprotein incubation(4) Three washes(5) Anti-NP-HRP conjugate incubation (6) Three washes(7) Substrate Incubation
* Each sample is tested in duplicate in an antigen-coated well (even columns) and in an adjacent non-coated well (odd columns). Results are calculated with respect to the corrected sample oD value (even well oD – odd well oD).
Associated Products
Rift Valley Fever IgM-positive freeze-dried serumProduct Code MRI-RIFTIGM
Format 0.5 ml vial (freeze-dried)
Description Freeze-dried anti-RVFV recombinant NP ovine serum to be used as internal reference material for quality control
40
Ruminants
SCHmALLENbERG ViRuS
Indirect ELISA kit for the detection of antibodies directed against the Schmallenberg virus (SBV) nucleoprotein (NP) in serum and plasma from multiple species, including cattle, sheep and goats. Please contact IDvet for use on other species.
ID Screen® Schmallenberg virus Indirect Multi-species
Method Indirect ELISA
Species Ruminants and other species
Specimens Serum, plasma
Coated antigen Recombinant SBV nucleoprotein antigen
Conjugate Anti-multi-species IgG-HRP conjugate (concentrated 10X)
Specifications
Ordering information
The first commercial ELISA for SBV serology
High sensitivity and specificity in external validation studies
Approved by the ANSES (France) and FLI (Germany) laboratories
Automatable and therefore suited to high throughput testing
Screening and confirmation formats available
* Each sample is tested in duplicate in a coated well (even columns) and in an adjacent non-coated well (odd columns). Results are calculated with respect to the corrected sample oD value (even well oD – odd well oD).
Confirmation format*
Product code SBVB-4P SBVB-10P
Kit format 4 plates (bi well) 10 plates (bi well)
Reactions 192 480
Plate format 6 x 16 well strips
Screening format
Product code SBVS-2P SBVS-4P
Kit format 2 plates 4 plates
Reactions 192 384
Plate format 12 x 8-well strips
References
(1) Jack C., Anstaett O, Adams J, Noad R, Browlie J., Mertens P. Evidence of seroconversion to SBV in camelids. Veterinary Record 2012 170:603 doi: 10.1136/vr.e3939
(2) Kaba et al. Schmallenberg Virus Antibodies Detected in Poland. Transboundary and Emerging Diseases. Rapid Communication. 2012.
(3) Linden A, Desmecht D, Volpe R, Wirtgen M, Pirson J, Paternostre J, et al. Epizootic Spreads of Schmallenberg virus among wild cervids, Belgium, fall 2011. Emerg Infect Dis (Internet). 2012 Dec.
(4) Bréard E, Lara E, Comtet L, Viarouge C, Doceul V, et al. (2013) Validation of a Commercially Available Indirect Elisa Using a Nucleocapside Recombinant Protein for Detection of Schmallenberg Virus Antibodies. PLoS ONE 8(1): e53446. doi:10.1371/journal.pone.0053446
Schmallenberg virus positive freeze-dried serumProduct Code MRI-SBV
Format 0.5 ml vial (freeze-dried)
Description Freeze-dried positive bovine serum to be used as internal reference material for quality control.
Associated Products
Negative bovine freeze-dried serumProduct Code MRINEG-BOV
Format 0.5 ml vial (freeze-dried)
Description Negative freeze-dried bovine serum to be used as internal reference material for quality control.
41
SCHmALLENbERG ViRuS
Competitive ELISA for the detection of antibodies directed against the Schmallenberg virus (SBV) nucleoprotein (NP) in serum or plasma from multiple-species .
ID Screen® Schmallenberg virus Competition Multi-species
Specifications
The only competitive ELISA for SBV serology
Applicable to multiple species, including cattle, sheep and goats
Improved sensitivity for sera with low VNT titres
Earlier detection of seroconversion
Improved specificity
Robust and easy-to-use: the conjugate is supplied ready-to-use, and results are obtained in 90 minutes
Product code SBVC-2P SBVC-4P
Kit format 2 plates 4plates
Reactions 192 384
Plate format 12 x 8 well strips
Ordering information
Method Competitive ELISA
Species Ruminants and other species
Specimens Serum and plasma
Coated antigen Recombinant SBV nucleoprotein antigen
Conjugate Ready-to-use anti-SBV nucleoprotein-HRP conjugate
Schmallenberg virus positive freeze-dried serumProduct Code MRI-SBV
Format 0.5 ml vial (freeze-dried)
Description Freeze-dried positive bovine serum to be used as internal reference material for quality control.
Associated Products
Negative bovine freeze-dried serumProduct Code MRINEG-BOV
Format 0.5 ml vial (freeze-dried)
Description Negative freeze-dried bovine serum to be used as internal reference material for quality control.
(1) Comtet L. & Pourquier P. (IDvet, France). ID Screen® Schmallenberg virus Competition Multi-species: validation of a competitive ELISA for the detection of antibodies directed against the Schmallenberg virus nucleoprotein. Poster presented at the Epizone 7th Annual meeting, brussels, belgium, 2013.
References
42
Ruminants
SCHmALLENbERG ViRuS
Indirect ELISA kit for the detection of antibodies directed against the Schmallenberg virus (SBV) nucleoprotein (NP) in ruminant individual and bulk milks.
ID Screen® Schmallenberg virus Milk Indirect
Specifications
The only commercial ELISA for the detection of SBV NP antibodies in milk
Ideal for monitoring the spread of the virus in disease-free regions
Cost-effective: the use of milk samples makes sample collection easier and more economical.
Accurate, robust and reliable : high specificity and sensitivity, and excellent correlation with serumtesting
Product code SBVMILK-2P SBVMILK-4P
Kit format 2 plates 4plates
Reactions 192 384
Plate format 12 x 8 well strips
Ordering information
Method Indirect ELISA
Species Bovine, ovine and caprine
Specimens Individual or bulk milks
Coated antigen Recombinant SBV antigen
Conjugate Anti-multi-species IgG-HRP conjugate(concentrated 10X)
43
Ordering information
References
Sheep (1) Proctor AF et al. Detection of antibodies to Toxoplasma gondii in serum from experimentally infected pregnant ewes. University College Dublin, 2008(2) Mangili, P.M. et al. Development and evaluation of the performance of an in-house ELISA to be used for the indirect diagnosis of Toxoplasmosis in sheep. Poster presented at the SIDILV meeting in Parma, Italy, 2009.
Swine(3) Hotea, I. et al. Seroprevalence of Toxoplasma gondii infection in pigs reared in intensive system from Timis country. Lucrari Stiintice Medicina veterinara Vol. XLIII(1), 2010, Timisoara.(4) Görlich, K. et al. Validation, optimisation and standardisation of an automated test system for monitoring and surveillance of Toxoplasmosa gondii in pigs. 2010. Poster presented at Nationales Zoonose Symposium, Berlin, 2009.(5) Gómez-Laguna, J. et al. Seroprevalence of zoonotic diseases in Iberian pigs. SUIS N° 74, Enero/Febrero 2011.
Deer(6) Gaffuri, A., et al. Toxoplasma gondii in wild boar and roe deer in Northern Italy: serosurvey and PCR-RFLP. 9th Ewda Conference, 2010.(7) Renzi, M. et al. Serological investigation on the spread of Toxoplasma gondii in roe deer (Capreolus capreolus) Emilia-Romagna. Poster presented at the 3rd National SIEF Congress, Torino, 2009.
Dogs and cats(8) Scarpulla, M. et al. Comparison of indirect immunofluorescence and ID Screen® Toxoplasmosis indirect ELISA for the detection of antibodies against Toxoplasma gondii in cat and dog sera. Poster presented at the WAVLD meeting, Madrid, 2009.
Wild & domestic animals(9) Roqueplo, C. et al. Toxoplasma gondii in wild and domestic animals from New Caledonia. Parasite, 2011, 18, 345-348.
ToxoPLASmoSiS
Indirect multi-species ELISA for the detection of anti-Toxoplasma gondii antibodies in serum, plasma or meat juice from multiple species, including ruminants, swine, and cats. Please contact IDvet for use with other species.
ID Screen® Toxoplasmosis Indirect Multi-species
Method Indirect ELISA
Species Ruminants, swine, dogs and cats.
Specimens Serum, plasma or meat juice
Coated antigen Toxoplasma gondii P30 antigen
Conjugate Anti-multi-species IgG-HRP conjugate (concentrated 10X)
Specifications
Easy-to-use, with results in 90 minutes
Versatile protocol for serum, plasma or meat juice, and applicable to multiple species, including ruminants, swine, dogs and cats
Product code TOXOS-MS-2P
Kit format 2 plates
Reactions 192
Plate format 12 x 8-well strips
44
Birds: Bertran, K. et al. Pathogenesis and transmissibility of highly (H7N1) and low (H7N9) pathogenic avian influenza virus infection in red-legged partridge (Alectoris rufa). Veterinary Research 2011, 42:24.Dundon, W.G., Terregino, C., Tuttoilmondo V., Pizzuto, M., Busani L., Mancin, M., Cattoli, G., Capua I.. Preliminary validation of a commercial Avian Influenza N1 antibody competitive Elisa kit that can be used as part of a Diva strategy. Epizone 2007, Poland.Molia, S. et al. Avian influenza in backyard poultry of the Mopti region, Mali. Trop Anim Health Prod. 2009. Terregino, C. Evaluation of sensitivity and specificity of a commercial competitive avian influenza type A antibody ELISA kit, the OIE-FAO and National Reference Laboratory for Newcastle Disease and Avian Influenza, IZS delle Venezie, Legnaro (Padova), Italy.Horses: Kittelberger, R., McFadden, A.M.J., Hannah, M.J., Jenner, J., Bueno, R., Vait, J., Kirkland, P.D., Delbridge, G., Heine, H.G., Selleck, P.W., Pearce, T.W., Pigott, C.J., O’Keefe, J.S., Comparative evaluation of four competitive/blocking ELISAs for the detection of influenza A antibodies in horses, Veterinary Microbiology (2010), doi:10.1016/j.vetmic.2010.08.014.Swine :Busquets, N. et al. Experimental infection with H1N1 European swine influenza virus protects pigs from an infection with the 2009 pandemic H1N1 human influenza virus. Vet. Res (2010) 41:74. Lange, E. et al. Pathogenesis and transmission of the novel swine-origin influenza virus A/H1N1 after experimental infection of pigs. Journal of General Virology (2009), 90, 2119-2123.Dogs:7 De Benedictis, P. et al. A diagnostic algorithm for detection of antibodies to influenza A viruses in dogs in Italy (2006-2008). J. Vet Diagn Invest 22:914-920 (2010).
45
EQuiNE
46
EQUINE
Product code EIAED-1P EIAED-4P
Kit format 1 plate 4 plates
Reactions 96 384
Plate format 12 x 8-well strips
EQuiNE iNFECTiouS ANEmiA
Double antigen ELISA kit for the detection of antibodies directed against the p26 protein of the Equine Infectious Anemia Virus (EIAV) in horse serum and plasma.
ID Screen® Equine Infectious Anemia Double Antigen
Associated Products
Method Double antigen ELISA
Species Equine
Specimens Serum and plasma
Coated antigen p26 recombinant antigen
Conjugate p26 antigen-HRP conjugate (concentrated 10X)
Highly sensitive and specific
Calibrated to detect the OIE standard positive anti-EIAV serum
Fast and easy-to-use, with results in 90 minutes
EIA positive freeze-dried serumProduct Code MRI-EIA
Format 1 ml vial (freeze-dried)
Description Freeze-dried positive horse serum to be used as internal reference material for quality control.
Specifications
Ordering information
References
Pourquier, P. et al. Validation of the ID Screen® Equine Infectious Anemia Double Antigen ELISA. Poster presented at the 2010 EAVLD meeting, Lelystad, The Netherlands.
47
Associated Products
Product code EIA-AGID EIA-AGID-NOGEL
Kit format Includes agar gel Agar gel not included
Reactions 200 200
EQuiNE iNFECTiouS ANEmiA
Agar gel immunodiffusion (AGID) test for the detection of EIA anti-p26 antibodies in equine serum.
IDvet EIA AGID
Method AGID (Coggins-test)
Species Equine
Specimens Serum
Antigen EIAV p26
Clear, bright lines for easy reading
OIE-prescribed protocol1
24-month shelf-life
May be purchased with or without agar gel (the iDvet agar gel allows for clearer precipitation lines)
Specifications
Ordering information
References
OIE Terrestrial Manual 2013; 2.5.6; Equine Infectious Anemia
EIA AGID CUTTERProduct Code EIACUTTER
Format As per OIE specifications: • A seven-well pattern is used with one centre well encircled by 6 wells• The wells are 2.4 mm apart and 5.3 mm in diameter
48
EQUINE
49
Product code EVAB-4P
Kit format 4 plates (Biwell format)*
Reactions 192
Plate format 6 x 16-well strips
EQuiNE ViRAL ARTERiTiS
Indirect confirmation ELISA kit for the detection of anti-EVA antibodies in horse serum and plasma.
ID Screen® Equine Viral Arteritis Indirect*
Associated Products
Method Indirect ELISA
Species Equine
Specimens Serum and plasma
Coated antigen Specific EVA peptide
Conjugate Anti-horse IgG-HRP conjugate (concentrated 10X)
Compatible with cytotoxic sera1 which cannot be tested with the virus neutralization test (VNT)
Easy-to-use, with results in just over 2 hours
EVA positive freeze-dried serumProduct Code MRI-EVA
Format 0.5 ml vial (freeze-dried)
Description Freeze-dried positive horse serum to be used as internal reference material for quality control.
Specifications
Ordering information
References
(1) Legrand, L., Pitel, P.H., Fotier, G., Pronost, S. Testing for antibodies to equine arteritis virus. The Veterinary Record, April 4th, 2009.
* Each sample is tested in duplicate in a coated well (even columns) and in an adjacent non-coated well (odd columns). Results are calculated with respect to the corrected sample oD value (even well oD – odd well oD).
* Not available in France
50
EQUINE
Product code FLUACA-2P FLUACA-4P FLUACA-10P
Kit format 2 plates 4 plates 10 plates
Reactions 192 384 960
Plate format 12 x 8-well strips
iNFLuENzA A
Competitive ELISA kit for the detection of anti-nucleoprotein (NP) antibodies. Detects antibodies against all Influenza A subtypes and antigenic variants in multiple species thanks to the use of a monoclonal antibody against a highly conserved epitope of the Influenza A virus nucleoprotein.
ID Screen® Influenza A Antibody Competition Multi-species
Method Competitive ELISA
Species Multiple species, including horses, birds, swine and others.
Specimens Serum and plasma
Coated antigen Influenza A nucleoprotein (NP)
Conjugate Anti-NP-HRP conjugate (concentrated 10X)
Highly sensitive and specific
Convenient multi-species test applicable to birds, horses, swine and others
Easy-to-use, with results in under 2 hours
Specifications
Ordering information
References
Horses: (1) Kittelberger, R., McFadden, A.M.J., Hannah, M.J., Jenner, J., Bueno, R., Vait, J., Kirkland, P.D., Delbridge, G., Heine, H.G., Selleck, P.W., Pearce, T.W., Pigott, C.J., O’Keefe, J.S., Comparative evaluation of four competitive/blocking ELISAs for the detection of influenza A antibodies in horses, Veterinary Microbiology (2010), doi:10.1016/j.vetmic.2010.08.014.Birds: (2) Bertran, K. et al. Pathogenesis and transmissibility of highly (H7N1) and low (H7N9) pathogenic avian influenza virus infection in red-legged partridge (Alectoris rufa). Veterinary Research 2011, 42:24.(3) Dundon, W.G., Terregino, C., Tuttoilmondo V., Pizzuto, M., Busani L., Mancin, M., Cattoli, G., Capua I.. Preliminary validation of a commercial Avian Influenza N1 antibody competitive Elisa kit that can be used as part of a Diva strategy. Epizone 2007, Poland.(4) Molia, S. et al. Avian influenza in backyard poultry of the Mopti region, Mali. Trop Anim Health Prod. 2009. (5) Terregino, C. Evaluation of sensitivity and specificity of a commercial competitive avian influenza type A antibody ELISA kit, the OIE-FAO and National Reference Laboratory for Newcastle Disease and Avian Influenza, IZS delle Venezie, Legnaro (Padova), Italy.Swine :(6) Busquets, N. et al. Experimental infection with H1N1 European swine influenza virus protects pigs from an infection with the 2009 pandemic H1N1 human influenza virus. Vet. Res (2010) 41:74. (7) Lange, E. et al. Pathogenesis and transmission of the novel swine-origin influenza virus A/H1N1 after experimental infection of pigs. Journal of General Virology (2009), 90, 2119-2123.Dogs:(8) De Benedictis, P. et al. A diagnostic algorithm for detection of antibodies to influenza A viruses in dogs in Italy (2006-2008). J. Vet Diagn Invest 22:914-920 (2010).
51
Product code INFLAG-2P
Kit format 2 plates
Reactions 192
Plate format 96 individual wells
iNFLuENzA A
Capture ELISA for the detection of the Influenza A nucleoprotein (NP).
ID Screen® Influenza A Antigen Capture
Method Sandwich ELISA
Species Avian, porcine, equine
Specimens -Broncho-pulmonary liquids from pigs,horses and birds.-Bird cloacae swab samples.-Bird faeces.
Coated antibody Anti-nucleoprotein (NP) monoclonalantibody
Conjugate Anti-NP-HRP conjugate (concentrated 10X)
High sensitivity and specificity
Cost-effective for the analysis of large sample numbers
Applicable to multiple species, including horses, birds and swine - please contact iDvet for more information
Efficient and robust on complex samples (faeces and cloacae swabs) which can generate false positive results with immunochromatographic tests
Easy-to-use and rapid, with results in under 1 hour
Specifications
Ordering information
52
EQUINE
Product code STREPTOS-2P
Kit format 2 plates
Reactions 192
Plate format 12 x 8-well strips
STRANGLES / STREPToCoCCuS EQui
Indirect ELISA kit for the detection of anti-Streptococcus equi in equine serum and plasma.
ID Screen® Streptococcus equi Indirect
Associated Products
Method Indirect ELISA
Species Horses
Specimens Serum and plasma
Coated antigen Recombinant SeM
Conjugate Anti-horse IgG-HRP conjugate (concentrated 10X)
Easy-to-use, standardized assay, with results in just over 2 hours
Qualitative protocol to detect previous exposure to S. equi
Quantitative protocol to assess SeM-specific antibody levels (horses with high SeM-specific serum antibodies may be predisposed to purpura when vaccinated for S. equi subsp equi with an attenuated live intranasal S. equi vaccine 1,2,3).
Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes colour upon addition of the sera
Streptococcus equi positive freeze-dried serumProduct Code MRI-STREPTO
Format 0.5 ml vial (freeze-dried)
Description Freeze-dried positive horse serum to be used as internal reference material for quality control.
Specifications
Ordering information
References
(1) Boyle, Ashley G. Streptococcus equi subspecies equi Infection (Strangles) in Horses. Vetlearn.com March 2011. Compendium: Continuing education for veterinarians. E1-8.
(2) Sweeney CR, Timoney JF, Newton JR, Hines MT. Streptococcus equi infections in horses: guidelines for treatment, control and prevention of strangles. J Vet Intern Med 2005:19:123-134.
(3) Timoney, JF. Equine strangles. Proc 45th Annu Meet AAEP 1999:31-37.
53
Product code TRICHIS-MS-2P
Kit format 2 plates
Reactions 192
Plate format 12 x 8-well strips
TRiCHiNELLoSiS
Indirect multi-species ELISA kit for the detection of anti-Trichinella antibodies (T. spiralis, pseudospiralis, britovi and nativa) in serum, plasma or meat juice from pigs, wild boar and horses. Please contact IDvet for use in other species.
ID Screen® Trichinella Indirect Multi-species
Method Indirect ELISA
Species Multiple species, including pigs , wild boar, horses and others
Specimens Serum, plasma or meat juice
Coated antigen Trichinella E/S antigen
Conjugate Anti-multi-species-HRP conjugate (concentrated 10X)
Highly sensitive and specific
Applicable to multiple species, including pigs, wild boar and horses
Serum, plasma and meat juice protocols
Specifications
Ordering information
54
EQUINE
Product code WNC-1P WNC-2P
Kit format 1 plate 2 plates
Reactions 96 192
Plate format 12 x 8-well strips
WEST NiLE ViRuS
Competitive ELISA kit for the detection of anti-pr-E antibodies in multiple species. The monoclonal antibody used in the kit cross-reacts with viruses from the Japanese Encephalitis Viruses (JEV) and the Tick-Borne Encephalitis Viruses (TBEV) serocomplex. Please contact IDvet for more information.
ID Screen® West Nile Competition Multi-species
Associated Products
Method Competitive ELISA
Species Equine & avian – please contact IDvet for use on other species
Specimens Serum or plasma
Coated antigen pr-E antigen
Conjugate Anti-pr-E-HRP conjugate (concentrated 10X)
Highly sensitive and specific detection of WNV antibodies
Applicable to multiple species, including horses, birds and others.
Rapid and convenient protocol, with ready-to-use reagents and results in just over 2 hours
West Nile IgG positive freeze-dried serumProduct Code MRI-WN
Format 1 ml vial (freeze-dried)
Description Freeze-dried positive horse serum to be used as internal reference material for quality control.
Specifications
Ordering information
References
(1) OIE Terrestrial Manual 2009; 2.1.20; West Nile Fever.
Horses:
(2) Van Maanen, Kees et al. Surveillance of West Nile Virus incursions in the Netherlands: Validation of antibody detecting ELISAs in horses. Oral presentation at the 2010 Epizone meeting in Saint Malo, France.
(3) Wernery, U. et al. West Nile Fever in the United Arab Emirates. Wildlife in the Middle East. Vol. 2, Issue 3, December 2007.
(4) Ziegler et al. Monitoring of West Nile Virus Infections in Germany. Zoonoses and Public Health 59 (Suppl. 2) (2012) 95-101.
Birds:
(5) Diaz-Sanchez, S. et al. Use of a multispecies competitive ELISA for investigation of exposure to West Nile virus in multiple species of captive and wild birds. Presented at the WAVLD meeting, Madrid 2009.
(6) Garcia-Bocanegra et al. Serosurvey of West Nile Virus and Other Flaviviruses of the Japanese Encephalitis Antigenic Complex in Birds from Andalusia, Southern Spain. Vector-Borne and Zoonotic Diseases. Volume 11, Number 0, 2011.
(7) Ziegler et al. Pathogenesis of West Nile virus lineage 1 and 2 in experimentally infected large falcons. Vet. Microbiol. (2012),http://dx.doi.org/10.1016/j.vetmic.2012.07.041.
(8) Yipici et al. Serologic Investigation for West Nile Virus Infection in Commerical Domestic Chickens (Gallus gallus domesticus). Journalof Animal and Veterinary Advances 11 (13):221-2214,2012
Other species:(9) Lan et. al. 2011. Serological evidence of West Nile virus in dogs and cats in China; Arch Virol (2011) 156:893-895.
55
Product code WNIGM-1P WNIGM-4P
Kit format 1 plate (biwell format*)
4 plates (biwell format*)
Reactions 48 192
Plate format 6 x 16-well strips
WEST NiLE ViRuS
IgM Antibody Capture ELISA (IMAC) kit for the detection of anti-pr-E IgM antibodies in horse serum and plasma. The detection of IgM antibodies indicates recent infection and WNV circulation. The monoclonal antibody used in the kit cross-reacts with viruses from the Japanese Encephalitis Viruses (JEV) and the Tick-Borne Encephalitis Viruses (TBEV) serocomplex. Please contact IDvet for more information.
ID Screen® West Nile IgM Capture
Associated Products
Method IMAC (IgM Antibody Capture) ELISA
Species Equine
Specimens Serum and plasma
Coated antibody Anti-horse IgM polyclonal antibody
Antigen Ready-to-use WNV pr-E antigen
Conjugate Anti-WNV pr-E HRP conjugate (10X)
Protocol* (1) Sample Incubation(2) Three washes(3) WNV ready-to-use antigen incubation(4) Anti-WNV prE-HRP conjugate incubation(5) Three washes(6) Substrate Incubation: 15 min
Sensitive and specific detection of WNV igm antibodies, indicators of recent infection
Rapid and convenient : all reagents are supplied ready-to-use, samples are diluted directly in the plate (no pre-dilutions required), and all reagents may be transported at room temperature and stored at 4°C.
West Nile IgM positive freeze-dried serumProduct Code MRI-WNIGM
Format 0.5 ml vial (freeze-dried)
Description Freeze-dried IgM-positive horse serum to be used as internal reference material for quality control.
Specifications
Ordering information
References
(1) Zeller, H.G. and Schuffenecker, I. West Nile Virus: An overview of its spread in Europe and the Mediterranean basin in contrast to its spread in the Americas. Eur J Clin Microbiol Infect Dis (2004) 23:147-156
(2) OIE Terrestrial Manual 2008 ; 2.1.20; West Nile Fever.
* Samples are deposited in duplicate in adjacent even and odd-numbered wells; the WNV pr-E is added only to even-numbered wells. Results are calculated with respect to the corrected sample oD value (even well oD – odd well oD).
57
AViAN
58
AVIAN
Product code IBVS-5P IBVS-10P
Kit format 5 plates 10 plates
Reactions 480 960
Plate format 12 x 8-well strips
iNFECTiouS bRoNCHiTiS
Indirect ELISA for the detection of antibodies against the Infectious Bronchitis Virus (IBV) in chicken sera.
May be used to detect infection or to quantify IBV antibody levels in vaccinated birds.
Note: The same convenient protocol (buffers, incubation times and temperatures) is used for most ID Screen® poultry kits.
ID Screen® Infectious Bronchitis Indirect
Associated Products
Method Indirect ELISA
Species Chicken
Specimens Serum
Coated antigen IBV purified antigen
Conjugate Anti-chicken-HRP conjugate (concentrated 10X)
Detects all IBV variants
High specificity and very low background
Short incubation times: results in 75 minutes (30-30-15 protocol)
Comprehensive data analysis with the iDSoft™ program
Freeze-dried positive and negative reference sera available as internal controls
The stop solution no longer contains H2SO4, meaning that it is less corrosive
IBV positive freeze-dried serumProduct Code MRI-IBV
Format 0.25 ml vial (freeze-dried)
Description Freeze-dried positive chicken serum to be used as internal reference material for quality control.
SPF negative freeze-dried serumProduct Code MRINEG-BIRD
Format 0.5 ml vial (freeze-dried)
Description Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.
Specifications
Ordering information
59
Product code IBVC-2P
Kit format 2 plates
Reactions 192
Plate format 12 x 8-well strips
iNFECTiouS bRoNCHiTiS
Competitive ELISA kit for the detection of anti-IBV NP antibodies in chicken serum and egg yolk.
ID Screen® Infectious Bronchitis Competition
Associated Products
Method Competitive ELISA
Species Chickens
Specimens Serum or egg yolk
Coated antigen IBV purified antigen
Conjugate Anti-IBV-HRP conjugate (concentrated 10X)
High sensitivity and specificity
Protocols for serum and egg yolk
Earlier detection of infected animals
The stop solution no longer contains H2SO4, meaning that it is less corrosive
IBV positive freeze-dried serumProduct Code MRI-IBV
Format 0.25 ml vial (freeze-dried)
Description Freeze-dried positive chicken serum to be used as internal reference material for quality control.
SPF negative freeze-dried serumProduct Code MRINEG-BIRD
Format 0.5 ml vial (freeze-dried)
Description Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.
Specifications
Ordering information
60
AVIAN
Product code IBDS-5P IBDS-10P
Kit format 5 plates 10 plates
Reactions 480 960
Plate format 12 x 8-well strips
iNFECTiouS buRSAL DiSEASE / GumboRo DiSEASE
Indirect ELISA for the detection of antibodies against the Infectious Bursal Disease Virus (IBDV) in chicken sera.
May be used to monitor the flock’s immune status, to quantify IBD antibody levels pre- and post-vaccination, or to determine the best time for vaccination by detecting maternal antibodies.
Note: The same convenient protocol (buffers, incubation times and temperatures) is used for most ID Screen® poultry kits.
ID Screen® IBD Indirect
Associated Products
Method Indirect ELISA
Species Chicken
Specimens Serum
Coated antigen IBD purified antigen
Conjugate Anti-chicken-HRP conjugate (concentrated 10X)
High specificity and very low background
Short incubation times: results in 75 minutes (30-30-15 protocol)
Comprehensive data analysis with the iDSoft™ program
Freeze-dried positive and negative reference sera available as internal controls
The stop solution no longer contains H2SO4, meaning that it is less corrosive
IBD positive freeze-dried serumProduct Code MRI-IBDS
Format 0.5 ml vial (freeze-dried)
Description Freeze-dried positive chicken serum to be used as internal reference material for quality control.
SPF negative freeze-dried serumProduct Code MRINEG-BIRD
Format 0.5 ml vial (freeze-dried)
Description Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.
Specifications
Ordering information
61
Product code IBDVP2-5P IBDVP2-10P
Kit format 5 plates 10 plates
Reactions 480 960
Plate format 12 x 8-well strips
iNFECTiouS buRSAL DiSEASE / GumboRo DiSEASE
Indirect ELISA for the detection of anti-IBDV VP2 antibodies in chicken sera.
May be used to quantify IBD antibody levels pre- and post-vaccination.
This test is designed to be used with animals vaccinated with recombinant VP2 vector vaccines.
ID Screen® IBD VP2
Associated Products
Method Indirect ELISA
Species Chicken
Specimens Serum
Coated antigen Recombinant VP2 antigen
Conjugate Anti-immunoglobulin-HRP conjugate (concentrated 10X)
IBD VP2 positive freeze-dried serumProduct Code MRI-IBDVP2
Format 0.5 ml vial (freeze-dried)
Description Freeze-dried VP2 positive chicken serum to be used as internal reference material for quality control.
Specifications
Ordering information
May be used with animals vaccinated with recombinant VP2 vector vaccines
High specificity and very low background
Short incubation times: results in 75 minutes(30-30-15 protocol)
Comprehensive data analysis with the iDSoft™ program
Freeze-dried positive and negative reference sera available as internal controls
The stop solution no longer contains H2SO4, meaning that it is less corrosive
SPF negative freeze-dried serumProduct Code MRINEG-BIRD
Format 0.5 ml vial (freeze-dried)
Description Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.
62
AVIAN
Product code ILTS-5P ILTS-10P
Kit format 5 plates 10 plates
Reactions 480 960
Plate format 12 x 8-well strips
iNFECTiouS LARyNGoTRACHEiTiS (iLT)
Indirect ELISA for the detection of antibodies directed against the avian Infectious Laryngotracheitis Virus (ILTV) in vaccinated and/or infected chicken sera.
ID Screen® ILT Indirect
Associated Products
Method Indirect ELISA
Species Chickens
Specimens Serum
Coated antigen ILTV purified antigen
Conjugate Anti-chicken-HRP conjugate (concentrated 10x)
High specificity and analytical sensitivity
Comprehensive data analysis with the iDSoft™ program
Freeze-dried positive and negative reference sera available as internal controls
The stop solution no longer contains H2SO4, meaning that it is less corrosive
ILT positive freeze-dried serumProduct Code MRI-ILT
Format 0.5 ml vial (freeze-dried)
Description Freeze-dried positive chicken serum to be used as internal reference material for quality control.
SPF negative freeze-dried serumProduct Code MRINEG-BIRD
Format 0.5 ml vial (freeze-dried)
Description Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.
Specifications
Ordering information
63
Product code FLUACA-2P FLUACA-4P FLUACA-10P
Kit format 2 plates 4 plates 10 plates
Reactions 192 384 960
Plate format 12 x 8-well strips
iNFLuENzA A
Competitive ELISA kit for the detection of anti-nucleoprotein (NP) antibodies. Detects antibodies against all Influenza A subtypes and antigenic variants thanks to the use of a monoclonal antibody against a highly conserved epitope of the Influenza A virus nucleoprotein.
ID Screen® Influenza A Antibody Competition Multi-species
Associated Products
Method Competitive ELISA
Species Multiple species, including birds, horses, swine and others
Specimens Serum and plasma
Coated antigen Influenza A nucleoprotein (NP)
Conjugate Anti-NP-HRP conjugate (concentrated 10X)
Influenza A positive freeze-dried serumProduct Code MRI-FLUACA/N1
Format 1 ml vial (freeze-dried)
Description Freeze-dried positive chicken serum to be used as internal reference material for quality control.
Specifications
Ordering information
Highly sensitive and specific
Convenient multi-species test applicable to birds, horses, swine and other species - please contact iDvet for more information
Easy-to-use, with results in under 2 hours
References
Birds: (1) Bertran, K. et al. Pathogenesis and transmissibility of highly (H7N1) and low (H7N9) pathogenic avian influenza virus infection in red-legged partridge (Alectoris rufa). Veterinary Research 2011, 42:24.(2) Dundon, W.G., Terregino, C., Tuttoilmondo V., Pizzuto, M., Busani L., Mancin, M., Cattoli, G., Capua I.. Preliminary validation of a commercial Avian Influenza N1 antibody competitive Elisa kit that can be used as part of a Diva strategy. Epizone 2007, Poland.(3) Molia, S. et al. Avian influenza in backyard poultry of the Mopti region, Mali. Trop Anim Health Prod. 2009. (4) Terregino, C. Evaluation of sensitivity and specificity of a commercial competitive avian influenza type A antibody ELISA kit, the OIE-FAO and National Reference Laboratory for Newcastle Disease and Avian Influenza, IZS delle Venezie, Legnaro (Padova), Italy.Horses: (5) Kittelberger, R., McFadden, A.M.J., Hannah, M.J., Jenner, J., Bueno, R., Vait, J., Kirkland, P.D., Delbridge, G., Heine, H.G., Selleck, P.W., Pearce, T.W., Pigott, C.J., O’Keefe, J.S., Comparative evaluation of four competitive/blocking ELISAs for the detection of influenza A antibodies in horses, Veterinary Microbiology (2010), doi:10.1016/j.vetmic.2010.08.014.Swine :(6) Busquets, N. et al. Experimental infection with H1N1 European swine influenza virus protects pigs from an infection with the 2009 pandemic H1N1 human influenza virus. Vet. Res (2010) 41:74. (7) Lange, E. et al. Pathogenesis and transmission of the novel swine-origin influenza virus A/H1N1 after experimental infection of pigs. Journal of General Virology (2009), 90, 2119-2123.Dogs:(8) De Benedictis, P. et al. A diagnostic algorithm for detection of antibodies to influenza A viruses in dogs in Italy (2006-2008). J. Vet Diagn Invest 22:914-920 (2010).
SPF negative freeze-dried serumProduct Code MRINEG-BIRD
Format 0.5 ml vial (freeze-dried)
Description Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.
64
AVIAN
Kit format 2 plates
Reactions 192
Plate format 12 x 8-well strips
iNFLuENzA A
Range of competitive ELISA kits for the detection of subtype-specific Influenza antibodies in avian serum or plasma.
ID Screen® Influenza subtype-specific kits
Method Competitive ELISA
Species Avian
Specimens Serum or plasma
Coated antigen Purified native inactivated subtype-specific antigen
Conjugate Anti-subtype-HRP Mab conjugate (concentrated 10X)
Specifications
Ordering information
May be used for DIVA diagnostics (Differentiation of infected and Vaccinated Animals)*
Applicable to both domestic & wild avian species
High sensitivity and specificity : validated by oiE labs, with excellent results
Easy-to-use, with results in under 2.5 hours
* For example, if vaccination is performed using a homologous he-magglutinin to the highly pathogenic field strain, but a heterologous neuraminidase subtype, serologic surveillance can be performed by testing for antibodies against the homologous N subtype as evidence of natural infection.
Associated Products
Influenza N1 positive freeze-dried chicken serum*
Product Code MRI-FLUACA/N1
Format 1 ml vial (freeze-dried)
SPF negative freeze-dried chicken serum*Product Code MRINEG-BIRD
Format 0.5 ml vial (freeze-dried)
Name Product codeID Screen® Influenza N1 Antibody Competition
FLUACN1-2P
ID Screen® Influenza N2 Antibody Competition
FLUACN2-2P
ID Screen® Influenza N9 Antibody Competition
FLUACN9-2P
ID Screen® Influenza H5 Antibody Competition
FLUACH5-2P
ID Screen® Influenza H7 Antibody Competition
FLUACH7-2P
Influenza H7 positive freeze-dried chicken serum*
Product Code MRI-FLUACH7
Format 0.5 ml vial (freeze-dried)
* Freeze-dried chicken sera to be used as internal reference mate-rial for quality control.
References
(1) Dundon, W.G,. Terregino, C., Tuttoilmondo, V., Pizzuto, M., Busani, L., Mancin, M., Cattoli, G., Capua, I. Preliminary validation of a commercial Avian Influenza N1 antibody competitive Elisa kit that can be used as part of a Diva strategy. Poster presented at EPIZONE 2007, Poland.
(2) Bötcher, L., Schöttker-Wegner, H.H., Nöckler, A. Summary of the German presentation at the AVID Meeting (September 15-17, 2010). Use of an H5-antibody ELISAs for the serological differentiation of AIV-antibodies. Niedersächsisches Landesamt für Verbrauchschutz und Lebensmittelsicherheit Veterinärinstitut, Oldenburg, Germany.
(3) Robles, F. et al. Implementation of « DIVA » Concept with specific ELISA kits ; When Subunit H5 Avian Influenza Vaccine is Used. World Academy of Science, Engineering and Technology 65 2010.
65
Method Sandwich ELISA
Species Multiple species, including birds, pigs, horses and others.
Specimens -Broncho-pulmonary liquids from pigs, horses and birds.-Bird cloacae swab samples.-Bird faeces.
Coated antibody Anti-nucleoprotein (NP) monoclonal antibody
Conjugate Anti-NP-HRP conjugate (concentrated 10X)
Product code INFLAG-2P
Kit format 2 plates
Reactions 192
Plate format 96 individual wells
Capture ELISA for the detection of the Influenza A nucleoprotein (NP).
ID Screen® Influenza A Antigen Capture
Associated Products
Specifications
Ordering information
High sensitivity and specificity
Cost-effective for the analysis of large sample numbers
Applicable to multiple species, including birds, horses and swine - please contact iDvet for more information
Efficient and robust on complex samples (faeces and cloacae swabs) which can generate false positive results with immunochromatographic tests
Easy-to-use and rapid, with results in under 1 hour
iNFLuENzA A
SPF negative freeze-dried serumProduct Code MRINEG-BIRD
Format 0.5 ml vial (freeze-dried)
Description Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.
66
AVIAN
Product code MG-5P MG-10P
Kit format 5 plates 10 plates
Reactions 480 960
Plate format 12 x 8-well strips
AViAN myCoPLASmoSiS
Recombinant protein-based indirect ELISA for the detection of anti-Mycoplasma gallisepticum antibodies in chicken and turkey sera to monitor the flock’s immune status.
Positive results should be confirmed by other methods such as Rapid Slide Agglutination (RSA).
Note: The same convenient protocol (buffers, incubation times and temperatures) is used for most ID Screen® poultry kits.
ID Screen® Mycoplasma gallisepticum Indirect
Associated Products
Method Indirect ELISA
Species Chicken and turkey
Specimens Serum
Coated antigen Recombinant MG antigen
Conjugate Anti-chicken-HRP conjugate (concentrated 10X)
MG positive freeze-dried serumProduct Code MRI-MG
Format 0.5 ml vial (freeze-dried)
Description Freeze-dried positive chicken serum to be used as internal reference material for quality control.
SPF negative freeze-dried serumProduct Code MRINEG-BIRD
Format 0.5 ml vial (freeze-dried)
Description Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.
Specifications
Ordering information
The use of recombinant proteins allows for improved specificity, low background, earlier detection of seroconversion, and higher analytical sensitivity
No cross-reactions with other mycoplasmas
Short incubation times: results in 75 minutes (30-30-15 protocol)
Applicable to chickens and turkeys
Comprehensive data analysis with the iDSoft™ program
Freeze-dried positive and negative reference sera available as internal controls
Detects anti-MG antibodies further to vaccination with the 6/85 strain
The stop solution no longer contains H2SO4, meaning that it is less corrosive
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Method Indirect ELISA
Species Chicken and turkey
Specimens Serum
Coated antigen Recombinant MS antigen
Conjugate Anti-chicken-HRP conjugate (concentrated 10X)
Product code MS-5P MS-10P
Kit format 5 plates 10 plates
Reactions 480 960
Plate format 12 x 8-well strips
Recombinant protein-based indirect ELISA for the detection of anti-Mycoplasma synoviae antibodies in chicken and turkey sera to monitor the flock’s immune status.
Positive results should be confirmed by other methods such as Rapid Slide Agglutination (RSA).
Note: The same convenient protocol (buffers, incubation times and temperatures) is used for most ID Screen® poultry kits.
ID Screen® Mycoplasma synoviae Indirect
Associated Products
Specifications
Ordering information
AViAN myCoPLASmoSiS
MS positive freeze-dried serumProduct Code MRI-MS
Format 1 ml vial (freeze-dried)
Description Freeze-dried positive chicken serum to be used as internal reference material for quality control.
SPF negative freeze-dried serumProduct Code MRINEG-BIRD
Format 0.5 ml vial (freeze-dried)
Description Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.
The use of recombinant proteins allows for improved specificity, low background, earlier detection of seroconversion, and higher analytical sensitivity
No cross-reactions with other mycoplasmas
Short incubation times: results in 75 minutes (30-30-15 protocol)
Applicable to chickens and turkeys
Comprehensive data analysis with the iDSoft™ program
Freeze-dried positive and negative reference sera available as internal controls
The stop solution no longer contains H2SO4, meaning that it is less corrosive
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AVIAN
Product code MG/MS-5P MG/MS-10P
Kit format 5 plates 10 plates
Reactions 480 960
Plate format 12 x 8-well strips
AViAN myCoPLASmoSiS
Recombinant protein-based indirect ELISA for the detection of anti-Mycoplasma gallisepticum and Mycoplasma synoviae antibodies in chicken and turkey sera to monitor the flock’s immune status.
Positive results should be confirmed using the separate ID Screen® MG or MS Indirect ELISAs, and then by other methods such as Rapid Slide Agglutination (RSA).
Note: The same convenient protocol (buffers, incubation times and temperatures) is used for most ID Screen® poultry kits.
ID Screen® MG/MS Indirect
Associated Products
Method Indirect ELISA
Species Chicken and turkey
Specimens Serum
Coated antigen MG and MS recombinant antigens
Conjugate Anti-chicken-HRP conjugate (concentrated 10X)
SPF negative serumProduct Code MRINEG-BIRD
Format 1 ml vial (freeze-dried)
Description Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.
Specifications
Ordering information
MS positive serumProduct Code MRI-MS
Format 1 ml vial (freeze-dried)
Description Freeze-dried positive chicken serum to be used as internal reference material for quality control.
MG positive serumProduct Code MRI-MG
Format 0.5 ml vial (freeze-dried)
Description Freeze-dried positive chicken serum to be used as internal reference material for quality control.
The use of recombinant proteins allows for improved specificity, low background, earlier detection of seroconversion, and higher analytical sensitivity
No cross-reactions with other mycoplasmas
Short incubation times: results in 75 minutes (30-30-15 protocol)
Applicable to chickens and turkeys
Comprehensive data analysis with the iDSoft™ program
Freeze-dried positive and negative reference sera available as internal controls
The stop solution no longer contains H2SO4, meaning that it is less corrosive
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70
AVIAN
Product code NDVS-5P NDVS-10P
Kit format 5 plates 10 plates
Reactions 480 960
Plate format 12 x 8-well strips
Indirect ELISA for the detection of antibodies against the Newcastle Disease Virus (NDV) in chicken sera.
May be used to detect infection or to quantify NDV antibody levels in vaccinated birds.
Note: The same convenient protocol (buffers, incubation times and temperatures) is used for most ID Screen® poultry kits.
ID Screen® Newcastle Disease Indirect
Associated Products
Method Indirect ELISA
Species Chicken
Specimens Serum
Coated antigen NDV purified antigen
Conjugate Anti-chicken-HRP conjugate (concentrated 10X)
NDV positive freeze-dried serumProduct Code MRI-NDV
Format 0.5 ml vial (freeze-dried)
Description Freeze-dried positive chicken serum to be used as internal reference material for quality control.
SPF negative freeze-dried serumProduct Code MRINEG-BIRD
Format 0.5 ml vial (freeze-dried)
Description Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.
Specifications
Ordering information
High specificity and very low background
Short incubation times: results in 75 minutes (30-30-15 protocol)
Excellent correlation with hemagglutination inhibition (Hi)
Comprehensive data analysis with the iDSoft™ program
Freeze-dried positive and negative reference sera available as internal controls
The stop solution no longer contains H2SO4, meaning that it is less corrosive
NEWCASTLE DiSEASE
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Product code NDVC-2P NDVC-5P NDVC-10P
Kit format 2 plates 5 plates 10 plates
Reactions 192 480 960
Plate format 12 x 8-well strips
Competitive ELISA kit for the detection of anti-NDV nucleoprotein antibodies in serum and egg yolk (domestic and wild avian species).
Applicable to both domestic and wild avian species, this test may be used to determine the immune status of individuals, flocks or populations.
ID Screen® Newcastle Disease Competition
Associated Products
Method Competitive ELISA
Species Avian (domestic and wild species)
Specimens Serum, plasma and egg yolk
Coated antigen NDV-purified antigen
Conjugate Anti-NDV-HRP conjugate (concentrated 10X)
NDV positive freeze-dried serumProduct Code MRI-NDV
Format 0.5 ml vial (freeze-dried)
Description Freeze-dried positive chicken serum to be used as internal reference material for quality control.
SPF negative freeze-dried serumProduct Code MRINEG-BIRD
Format 0.5 ml vial (freeze-dried)
Description Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.
Specifications
Ordering information
Multi-species test: the only commercial ELiSA applicable to both domestic and wild avian species
High sensitivity and specificity: • Earlier detection of seroconversion (7 dpi) compared with indirect ELiSAs • High analytical sensitivity • Detection of all strains • No cross-reactions with Avian Paramyxoviruses 3 (APmV-3).
Flexible and rapid protocol : applicable to serum, plasma and egg yolk samples, and results are obtained in just 70 minutes.
Used worldwide in government monitoring programs
The stop solution no longer contains H2SO4, meaning that it is less corrosive
NEWCASTLE DiSEASE
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AVIAN
Product code SALS-5P SALS-10P
Kit format 5 plates 10 plates
Reactions 480 960
Plate format 12 x 8-well strips
Indirect ELISA for the detection of anti-Salmonella group B and D antibodies in chicken sera.
Note: The same convenient protocol (buffers, incubation times and temperatures) is used for most ID Screen® poultry kits.
ID Screen® Avian Salmonella Indirect – Groups B & D
Associated Products
Method Indirect ELISA
Species Chicken
Specimens Serum
Coated antigen Purified Salmonella LPS
Conjugate Anti-chicken IgG-HRP conjugate (concentrated 10X)
SAL positive freeze-dried serumProduct Code MRI-SALS
Format 0.5 ml vial (freeze-dried)
Description Freeze-dried positive chicken serum to be used as internal reference material for quality control.
SPF negative freeze-dried serumProduct Code MRINEG-BIRD
Format 0.5 ml vial (freeze-dried)
Description Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.
Specifications
Ordering information
Very low background
Detects antibodies directed against Salmonella enteritidis (Se), S. thyphimurium (St), and the other avian salmonellas belonging to groups B and D.
Short incubation times with results in 75 minutes
Comprehensive data analysis with the iDSoft™ program
Freeze-dried positive and negative reference sera available as internal controls
The stop solution no longer contains H2SO4, meaning that it is less corrosive
SALmoNELLoSiS
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Product code WNC-1P WNC-2P
Kit format 1 plate 2 plates
Reactions 96 192
Plate format 12 x 8-well strips
WEST NiLE ViRuS
Competitive ELISA kit for the detection of anti-pr-E antibodies in multiple species. The monoclonal antibody used in the kit cross-reacts with viruses from the Japanese Encephalitis Viruses (JEV) and the Tick-Borne Encephalitis Viruses (TBEV) serocomplex. Please contact IDvet for more information.
ID Screen® West Nile Competition Multi-species
Associated Products
Method Competitive ELISA
Species Multiple species, including birds, horses, and others.
Specimens Serum or plasma
Coated antigen pr-E antigen
Conjugate Anti-pr-E-HRP conjugate (concentrated 10X)
Specifications
Ordering information
Highly sensitive and specific detection of WNV antibodies
Multi-species test for horses and birds
Rapid and convenient protocol, with ready-to-use reagents and results in just over 2 hours
References
(1) OIE Terrestrial Manual 2009; 2.1.20; West Nile Fever.
Birds:
(2) Diaz-Sanchez, S. et al. Use of a multispecies competitive ELISA for investigation of exposure to West Nile virus in multiple species of captive and wild birds. Presented at the WAVLD meeting, Madrid 2009.
(3) Garcia-Bocanegra et al. Serosurvey of West Nile Virus and Other Flaviviruses of the Japanese Encephalitis Antigenic Complex in Birds from Andalusia, Southern Spain. Vector-Borne and Zoonotic Diseases. Volume 11, Number 0, 2011.
(4) Ziegler et al. Pathogenesis of West Nile virus lineage 1 and 2 in experimentally infected large falcons. Vet. Microbiol. (2012),http://dx.doi.org/10.1016/j.vetmic.2012.07.041.
(5) Yipici et al. Serologic Investigation for West Nile Virus Infection in Commerical Domestic Chickens (Gallus gallus domesticus). Journalof Animal and Veterinary Advances 11 (13):221-2214,2012
Horses:
(6) Van Maanen, Kees et al. Surveillance of West Nile Virus incursions in the Netherlands: Validation of antibody detecting ELISAs in horses. Oral presentation at the 2010 Epizone meeting in Saint Malo, France.
(7) Wernery, U. et al. West Nile Fever in the United Arab Emirates. Wildlife in the Middle East. Vol. 2, Issue 3, December 2007.
(8) Ziegler et al. Monitoring of West Nile Virus Infections in Germany. Zoonoses and Public Health 59 (Suppl. 2) (2012) 95-101.
Other species:(9) Lan et. al. 2011. Serological evidence of West Nile virus in dogs and cats in China; Arch Virol (2011) 156:893-895.
SPF negative freeze-dried serumProduct Code MRINEG-BIRD
Format 0.5 ml vial (freeze-dried)
Description Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.
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75
SWiNE
76
SWINE
Product code APPS-2P
Kit format 2 plates
Reactions 192
Plate format 12 x 8-well strips
ACTiNobACiLLuS PLEuRoPNEumoNiAE
Indirect ELISA kit for the detection of antibodies against APP serotypes 1 through 12 in swine serum, plasma and meat juice.
ID Screen® APP Screening Indirect (serotypes 1 through 12)
Method Indirect ELISA
Species Swine
Specimens Serum, plasma or meat juice
Coated antigen APP antigens
Conjugate Anti-swine IgG–HRP conjugate (concentrated 10X)
Specifications
Ordering information
Screening of 12 APP serotypes in a single test. Serotyping may be performed on positive samples using the iD Screen® serotyping ELiSAs (opposite page).
Fast and easy-to-use, no sample pre-dilution required, and results in just 75 minutes
The same straightforward protocol is used for all ID Screen® APP ELISAs, making it easier to use several tests at once
Serum, plasma and meat juice protocols
References
Pourquier, P., Lesceu, S. Use of the ID Screen APP indirect ELISA range for quick identification and serotyping of Actinobacillus pleuropneumoniae in domestic pigs. Poster presented at the 6th International Symposium on Emerging and Re-emerging Pig Diseases, June 2011, Madrid, Spain.
Associated Products
APP positive freeze-dried sera
Product Code MRI-APP-1-9-11 MRI-APP-2 MRI-APP-3-8 MRI-APP-4-7 MRI-APP-5 MRI-APP-6 MRI-APP-10 MRI-APP-12
Format 0.25 ml vial (freeze-dried)
0.25 ml vial (freeze-dried)
1 ml vial (freeze-dried)
0.25 ml vial (freeze-dried)
0.25 ml vial (freeze-dried)
1 ml vial (freeze-dried)
0.25 ml vial (freeze-dried)
0.25 ml vial (freeze-dried)
Description Freeze-dried positive swine serum to be used as internal reference material for quality control.
APP negative freeze-dried serum Product Code MRINEG-APP
Format 0.5 ml vial (freeze-dried)
Description Freeze-dried negative swine serum to be used as internal reference material for quality control.
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References
Pourquier, P., Lesceu, S. Use of the ID Screen APP indirect ELISA range for quick identification and serotyping of Actinobacillus pleuropneumoniae in domestic pigs. Poster presented at the 6th International Symposium on Emerging and Re-emerging Pig Diseases, June 2011, Madrid, Spain.
Kit format 2 plates
Reactions 192
Plate format 12 x 8-well strips
ACTiNobACiLLuS PLEuRoPNEumoNiAE
Comprehensive range of indirect ELISAs for the detection of serotype-specific APP antibodies in swine serum, plasma and meat juice.
ID Screen® APP serotyping kits
Associated Products
Method Indirect ELISA
Species Swine
Specimens Serum, plasma or meat juice
Coated antigen APP serotype specific antigens
Conjugate Anti-swine IgG – HRP conjugate (concentrated 10X)
APP positive freeze-dried sera
Product Code MRI-APP-1-9-11 MRI-APP-2 MRI-APP-3-8 MRI-APP-4-7 MRI-APP-5 MRI-APP-6 MRI-APP-10 MRI-APP-12
Format 0.25 ml vial (freeze-dried)
0.25 ml vial (freeze-dried)
1 ml vial (freeze-dried)
0.25 ml vial (freeze-dried)
0.25 ml vial (freeze-dried)
1 ml vial (freeze-dried)
0.25 ml vial (freeze-dried)
0.25 ml vial (freeze-dried)
Description Freeze-dried positive swine serum to be used as internal reference material for quality control.
Specifications
Ordering information
High serotype-specificity and sensitivity
Easy-to-use, as there is no sample pre-dilution step, and results are obtained in just 75 minutes
The same fast and convenient protocol is used for the entire range of ID Screen® APP ELISAs, making it easier to use several tests at once
Name Product codeID Screen® APP 1-9-11 Indirect APPS1-9-11-2P
ID Screen® APP 2 Indirect APPS2-2P
ID Screen® APP 2-6 Indirect APPS2-6-2P
ID Screen® APP 2-6-12 Indirect APPS2-6-12-2P
ID Screen® APP 3-6-8 Indirect APPS3-6-8-2P
ID Screen® APP 4-7 Indirect APPS4-7-2P
ID Screen® APP 5 Indirect APPS5-2P
ID Screen® APP 8 Indirect APPS8-2P
ID Screen® APP 10 Indirect APPS10-2P
ID Screen® APP 12 Indirect APPS12-2P
APP negative freeze-dried serum Product Code MRINEG-APP
Format 0.5 ml vial (freeze-dried)
Description Freeze-dried negative swine serum to be used as internal reference material for quality control.
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SWINE
Product code AUJESZKYGB-4P
Kit format 4 plates
Reactions 384
Plate format 12 x 8-well strips
AuJESzky’S DiSEASE / PSEuDoRAbiES
Competitive ELISA for the detection of anti-gB antibodies. Detects antibodies against all Aujeszky’s Disease Virus (ADV) strains further to natural infection or vaccination.
ID Screen® Aujeszky gB Competition
Associated Products
Method Competitive ELISA
Species Swine (domestic pigs and wild boar)
Specimens Serum or plasma
Coated antigen Antigenic suspension of the Aujeszky Disease Virus (ADV)
Conjugate Anti-gB-HRP conjugate (concentrated 10X)
Specifications
Ordering information
High sensitivity and specificity
Meets OIE analytical sensitivity requirements1: calibrated against the OIE ADV-1 reference serum
Convenient and flexible : short (results within 2 hours) or overnight protocols
Automatable for high throughput testing
Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes colour upon addition of the sera
ADV positive freeze-dried serum (vaccinated animal)Product Code MRI-AUJGB
Format 1 ml vial (freeze-dried)
Description Freeze-dried positive porcine serum from a vaccinated animal. To be used as internal reference material for quality control. This serum gives a negative result on the ID Screen® Aujeszky gE Competition ELISA.
References
(1) OIE Terrestrial Manual 2008; 2.1.2; Aujeszky’s Disease.
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Method Competitive ELISA
Species Swine (domestic pigs and wild boar)
Specimens Serum or plasma
Coated antigen Antigenic suspension of the Aujeszky Disease Virus (ADV)
Conjugate Ready to use anti-gE-HRP conjugate
Product code AUJESZKYGE-4P
Kit format 4 plates
Reactions 384
Plate format 12 x 8-well strips
AuJESzky’S DiSEASE / PSEuDoRAbiES
Competitive ELISA for the detection of anti-gE antibodies in swine serum and plasma. Detects naturally-infected animals in a vaccinated population, provided that animals are vaccinated with a vaccine that does not express the gE protein. For use in ADV vaccination-eradication programs in combination with gE-deleted vaccines.
ID Screen® Aujeszky gE Competition
Specifications
Ordering information
Sensitive and specific detection of anti-gE antibodies
Meets OIE analytical sensitivity requirements1: calibrated against the OIE International Standard Reference Serum
May be used as a DIVA test (Differentiation of Infected and Vaccinated Animals)
Convenient and flexible protocol : ready-to-use conjugate, with short and overnight protocols
Automatable for high throughput testing
Associated Products
ADV positive freeze-dried serum (naturally-infected animal)Product Code MRI-AUJGE
Format 1 ml vial (freeze-dried)
Description Freeze-dried positive porcine serum from a naturally-infected animal. To be used as internal reference material for quality control. This serum is also positive on the ID Screen® Aujeszky gB Competition ELISA.
References
(1) OIE Terrestrial Manual 2008; 2.1.2; Aujeszky’s Disease.
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SWINE
Method Indirect ELISA
Species Swine (domestic pigs and wild boar)
Specimens Serum, plasma, meat juice or blood filter paper samples
Coated antigen Mix of P32, P62 and P72recombinant proteins
Conjugate Anti-multi-species-HRP concentratedconjugate (10X)
AFRiCAN SWiNE FEVER
Multi-antigen indirect ELISA kit for the detection of antibodies against P32, P62 and P72 of the ASFV in porcine serum, plasma, meat juice or blood filter paper samples.
ID Screen® African Swine Fever Indirect
Specifications
Ordering information
The only commercial ELISA based on 3 recombinant antigens : P32, P62, and P72
High sensitivity and specificity, with clear separation of positive and negative results
Convenient and versatile, with ready-to-use components and protocols for serum, plasma, meat juice and blood filter paper samples.
Blood filter paper samples may be tested in 96-well deep-well plates, making sample processing faster and less prone to mix-ups
Associated Products
ASF positive freeze-dried serumProduct Code MRI-ASF
Format 0.5 ml vial (freeze-dried)
Description Freeze-dried positive porcine serum to be used as internal reference material for quality control.
* Each sample is tested in duplicate in a coated well (even columns) and in an adjacent non-coated well (odd columns). Results are calculated with respect to the corrected sample oD value (even well oD – odd well oD).
Confirmation format*
Product code ASFB-4P
Kit format 4 plates (bi-well)
Reactions 192
Plate format 6 x 16 well strips
Screening format
Product code ASFS-2P ASFS-4P
Kit format 2 plates 4 plates
Reactions 192 384
Plate format 12 x 8-well strips
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bRuCELLoSiS
ID Screen® Brucellosis Serum Indirect Multi-species
Indirect ELISA for the detection of antibodies against Brucella abortus, melitensis or suis in bovine, ovine, caprine and porcine serum and plasma (individual samples or pools of up to 10).
Calibrated according to OIE specifications and Annex C of the European directive CEE 64/432 to correctly detect the OIEELISASPISS standard serum.
Straightforward, versatile test, with short and overnight incubations for individual serum or plasma samples, or pools of up to 10 samples.
Product code BRUS-MS-4P BRUS-MS-10P
Kit format 4 plates 10 plates
Reactions 384 960
Plate format 12 x 8-well strips
Method Indirect ELISA
Species Bovine, ovine, caprine and porcine. Please contact IDvet for use in other species.
Specimens Individual serum or plasma samples, or pools of up to 10 samples.
Coated antigen Purified Brucella LPS
Conjugate Anti-multi-species-IgG-HRP conjugate (concentrated 10X)
Specifications
Ordering information
References
(1) OIE Terrestrial Manual 2009; 2.4.3; Bovine brucellosis.
(2) Annex C of the European directive CEE 64/432
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SWINE
Product code CSFE2C-4P
Kit format 4 plates
Reactions 384
Plate format 12 x 8-well strips
CLASSiCAL SWiNE FEVER / HoG CHoLERA
Competitive ELISA kit for the detection of anti-E2 antibodies in swine serum or plasma.
ID Screen® Classical Swine Fever E2 Competition
Associated Products
Method Competitive ELISA
Species Swine (domestic pigs and wild boar)
Specimens Serum or plasma
Coated antigen Recombinant E2 glycoprotein
Conjugate Ready-to-use anti-E2-HRP conjugate
Specifications
Ordering information
Sensitive and specific detection of anti-E2 antibodies
Fast and convenient protocol : ready-to-use conjugate, and results in 90 minutes.
Classical Swine Fever positivefreeze-dried serumProduct Code MRI-CSFE2
Format 1 ml vial (freeze-dried)
Description Freeze-dried highly positive swine serum to be used as internal reference material for quality control.
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Method Indirect ELISA
Species Swine and other species. Please contact IDvet for more information.
Specimens Serum or plasma
Coated antigen Recombinant genotype 3 capsid
Conjugate Anti-multi-species IgG-HRP conjugate (concentra-ted 10X)
HEPATiTiS E
Indirect ELISA for the detection of anti-Hepatitis E antibodies in serum and plasma from swine and other species. The use of genotype 3 antigens makes the test ideally suited for use in swine, as genotype 3 is the genotype which infects pigs (HEV ELISAs for humans use genotype 1 antigens).
ID Screen® Hepatitis E Indirect Multi-species
Specifications
High sensitivity and specificity in swine, thanks to the use of genotype 3 antigens
Applicable to multiple species, and validated on swine. Please contact IDvet for more information
Straightforward and rapid, with results in 90 minutes
References
Eiden M., Schlosser M., Groschup J. (Friedrich-Loeffler-Institut, Insel Riems, Germany), Pourquier P., Comtet L. (IDvet). Preliminary validation data of a commercial multi-species ELISA kit for detection of anti-Hepatitis E Virus antibodies in swine. Poster presented at the 6th International Symposium on Emerging and Re-emerging Pig Diseases, June 2011, Madrid, Spain.
Product code HEVB-MS-4P
Kit format 4 plates
Reactions 192 (Biwell format*)
Plate format 6 x 16-well strips
Ordering information
* Each sample is tested in duplicate in a coated well (even columns) and in an ad-jacent non-coated well (odd columns). Results are calculated with respect to the corrected sample oD value (even well oD – odd well oD).
Associated Products
HEV positive freeze-dried serumProduct Code MRI-HEV
Format 1 ml vial (freeze-dried)
Description Freeze-dried positive swine serum to be used as internal reference material for quality control.
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SWINE
Product code FLUACA-2P FLUACA-4P FLUACA-10P
Kit format 2 plates 4 plates 10 plates
Reactions 192 384 960
Plate format 12 x 8-well strips
iNFLuENzA A
Competitive ELISA kit for the detection of anti-nucleoprotein (NP) antibodies. Detects antibodies against all Influenza A subtypes and antigenic variants thanks to the use of a monoclonal antibody against a highly conserved epitope of the Influenza A virus nucleoprotein.
ID Screen® Influenza A Antibody Competition Multi-species
Method Competitive ELISA
Species Multiple species, including swine, birds, horses and others
Specimens Serum and plasma
Coated antigen Influenza A nucleoprotein (NP)
Conjugate Anti-NP-HRP conjugate (concentrated 10X)
Specifications
Ordering information
Highly sensitive and specific
Convenient multi-species test applicable to birds, horses, swine and other species - please contact IDvet for more information
Cost-effective for the analysis of large sample numbers, particularly in comparison with Haemagglutination Inhibition (HI)
Easy-to-use, with results in less than 2 hours
References
Swine :(1) Busquets, N. et al. Experimental infection with H1N1 European swine influenza virus protects pigs from an infection with the 2009 pandemic H1N1 human influenza virus. Vet. Res (2010) 41:74. (2) Lange, E. et al. Pathogenesis and transmission of the novel swine-origin influenza virus A/H1N1 after experimental infection of pigs. Journal of General Virology (2009), 90, 2119-2123. Horses: (3) Kittelberger, R., McFadden, A.M.J., Hannah, M.J., Jenner, J., Bueno, R., Vait, J., Kirkland, P.D., Delbridge, G., Heine, H.G., Selleck, P.W., Pearce, T.W., Pigott, C.J., O’Keefe, J.S., Comparative evaluation of four competitive/blocking ELISAs for the detection of influenza A antibodies in horses, Veterinary Microbiology (2010), doi:10.1016/j.vetmic.2010.08.014.Birds: (4) Bertran, K. et al. Pathogenesis and transmissibility of highly (H7N1) and low (H7N9) pathogenic avian influenza virus infection in red-legged partridge (Alectoris rufa). Veterinary Research 2011, 42:24.(5) Dundon, W.G., Terregino, C., Tuttoilmondo V., Pizzuto, M., Busani L., Mancin, M., Cattoli, G., Capua I.. Preliminary validation of a commercial Avian Influenza N1 antibody competitive Elisa kit that can be used as part of a Diva strategy. Epizone 2007, Poland.(6) Molia, S. et al. Avian influenza in backyard poultry of the Mopti region, Mali. Trop Anim Health Prod. 2009. (7) Terregino, C. Evaluation of sensitivity and specificity of a commercial competitive avian influenza type A antibody ELISA kit, the OIE-FAO and National Reference Laboratory for Newcastle Disease and Avian Influenza, IZS delle Venezie, Legnaro (Padova), Italy.Dogs:(8) De Benedictis, P. et al. A diagnostic algorithm for detection of antibodies to influenza A viruses in dogs in Italy (2006-2008). J. Vet Diagn Invest 22:914-920 (2010).
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Method Sandwich ELISA
Species Multiple species, including swine, birds, horses and others.
Specimens -Broncho-pulmonary liquids from pigs, horses and birds.-Bird cloacae swab samples.-Bird faeces.
Coated antibody Anti-nucleoprotein (NP) monoclonal antibody
Conjugate Anti-NP-HRP conjugate (concentrated 10X)
Product code INFLAG-2P
Kit format 2 plates
Reactions 192
Plate format 96 individual wells
iNFLuENzA A
Capture ELISA for the detection of the Influenza A nucleoprotein (NP).
ID Screen® Influenza A Antigen Capture
Specifications
Ordering information
High sensitivity and specificity
Cost-effective for the analysis of large sample numbers
Applicable to multiple species, including birds, horses and swine - please contact IDvet for more information
Efficient and robust on complex samples (faeces and cloacae swabs) which can generate false positive results with immunochromatographic tests
Easy-to-use and rapid, with results in less than 1 hour
86
SWINE
Method Indirect ELISA
Species SwinePlease contact IDvet for use in other species
Specimens Serum, plasma or meat juice
Coated antigen Purified MAC extract
Conjugate Anti-multi-species IgG-HRP conjugate (concentrated 10X)
Product code MA-MS-4P
Kit format 4 plates
Reactions 384
Plate format 12 x 8-well strips
myCobACTERium AVium
Indirect multi-species ELISA for the detection of anti-Mycobacterium avium complex (MAC) antibodies in sera and plasma from swine.
ID Screen® Mycobacterium avium Indirect Multi-species
Specifications
Ordering information
Excellent specificity
This test is designed to detect circulation of M. avium subsp. hominissuis on the herd level (low test sensitivity for individuals)
Cost-effective for high throughput testing, notably in comparison to the incision of lymph nodes at slaughter
Convenient protocol, with ready-to-use components and results in 2 hours.
(1) Pourquier, P., Lesceu S. Preliminary validation of the ID Screen® Mycobacterium avium Multi-species Indirect ELISA. Poster presented at the AHVLA International Conference 2011 - Animal Diseases and their Consequences, Royal Holloway, England.
(2) Shade B et al. Diagnostic investigations on mycobacterial abortion in a sow herd. Poster presented at the 21st IPVS Congress, Vancouver, Canada, July, 2010.
References
Associated Products
Mycobacterium avium positive freeze-dried serumProduct Code MRI-MA
Format 1 ml vial (freeze-dried)
Description Freeze-dried positive swine serum to be used as internal reference material for quality control.
87
Method Indirect ELISA
Species Swine
Specimens Serum or plasma
Coated antigen Recombinant P46 of M. hyopneumoniae
Conjugate Anti-swine IgG-HRP conjugate (concentrated 10X)
Product code MHYOPS-2P
Kit format 2 plates
Reactions 192
Plate format 12 x 8-well strips
myCoPLASmA HyoPNEumoNiAE / ENzooTiC PNEumoNiAE / myCoPLASmAL PNEumoNiA oF SWiNE
Indirect ELISA kit for the detection of anti-P46 antibodies in swine serum and plasma.
ID Screen® Mycoplasma hyopneumoniae Indirect
Specifications
Ordering information
Accurate and reliable, with high specificity and sensitivity
Easy-to-use : results in 90 minutes
Associated Products
Mycoplasma hyopneumoniae positive freeze-dried serumProduct Code MRI-MHYOP
Format 1 ml vial (freeze-dried)
Description Freeze-dried positive swine serum to be used as internal reference material for quality control.
88
SWINE
Method Competitive ELISA
Species Swine
Specimens Serum
Coated antigen Purified PRRSV antigen
Conjugate Anti-nucleoprotein-HRP conjugate (concentrated 10X)
Product code PRRS-4P PRRS-10P
Kit format 4 plates 10 plates
Reactions 384 960
Plate format 12 x 8-well strips
PoRCiNE REPRoDuCTiVE AND RESPiRAToRy SyNDRomE (PRRS)
Competitive ELISA for the detection of PRRSV anti-nucleoprotein (ORF7) antibodies in swine serum samples. Detects antibodies against both the US and European strains.
ID Screen® PRRS Competition
Specifications
Ordering information
Detection of both US and European strains
Excellent specificity and sensitivity
Convenient protocol, with ready-to-use components and results in 2 hours
89
Product code SVDC-4P
Kit format 4 plates
Reactions 384
Plate format 12 x 8-well strips
SWiNE VESiCuLAR DiSEASE
5B7 Mab Competitive ELISA1,2 for the detection of antibodies directed against the SVD virus in porcine serum or plasma.
ID Screen® Swine Vesicular Disease Competition
Method Competitive ELISA
Species Swine
Specimens Serum or plasma
Coated antigen SVDV purified antigen
Conjugate Mab 5B7-HRP conjugate (concentrated 10X)
Specifications
Ordering information
High sensitivity and specificity, with clear separation of positive and negative results
Calibrated to detect the Primary Reference Serum EU-RS4, provided by the OIE Reference Laboratory, Pirbright, UK2
Convenient and rapid test, with ready-to-use components and results within 2 hours.
(1) Brocchi, E., Berlinzani, A., Gamba, D. & Simone, F. (1995). Development of two novel monoclonal antibody-based ELISAs for the detection of antibodies and the identification of swine isotypes against swine vesicular disease virus. J. Virol.Methods, 52, 155-67.
(2) OIE Terrestrial Manual 2013; 2.8.9; Swine Vesicular Disease.
References
Associated Products
SVD positive freeze-dried serumProduct Code MRI-SVD
Format 0.5 ml vial (freeze-dried)
Description Freeze-dried positive swine serum to be used as internal reference material for quality control.
90
SWINE
Method Indirect ELISA
Species Multiple species, including ruminants, swine, dogs, cats and others
Specimens Serum, plasma or meat juice
Coated antigen Toxoplasma gondii P30 antigen
Conjugate Anti-multi-species IgG-HRP conjugate (concentrated 10X)
Product code TOXOS-MS-2P
Kit format 2 plates
Reactions 192
Plate format 12 x 8-well strips
ToxoPLASmoSiS
Indirect multi-species ELISA for the detection of anti-Toxoplasma gondii antibodies in serum, plasma or meat juice from multiple species, including ruminants, swine, dogs and cats.
ID Screen® Toxoplasmosis Indirect Multi-species
Specifications
Ordering information
Easy-to-use, with results in 90 minutes
Versatile protocol for serum, plasma or meat juice
Applicable to multiple species, including ruminants, swine, dogs and cats
Associated Products
Toxoplasmosis positive freeze-dried serumProduct Code MRI-TOXO
Format 1 ml vial (freeze-dried)
Description Freeze-dried positive porcine serum (wild boar) to be used as internal reference material for quality control.
References
Swine(1) Hotea, I. et al. Seroprevalence of Toxoplasma gondii infection in pigs reared in intensive system from Timis country. Lucrari Stiintice Medicina veterinara Vol. XLIII(1), 2010, Timisoara.(2) Görlich, K. et al. Validation, optimisation and standardisation of an automated test system for monitoring and surveillance of Toxoplasmosa gondii in pigs. 2010. Poster presented at Nationales Zoonose Symposium, Berlin, 2009.(3) Gómez-Laguna, J. et al. Seroprevalence of zoonotic diseases in Iberian pigs. SUIS N° 74, Enero/Febrero 2011.Sheep(4) Proctor AF et al. Detection of antibodies to Toxoplasma gondii in serum from experimentally infected pregnant ewes. University College Dublin, 2008(5) Mangili, P.M. et al. Development and evaluation of the performance of an in-house ELISA to be used for the indirect diagnosis of Toxoplasmosis in sheep. Poster presented at the SIDILV meeting in Parma, Italy, 2009.Deer(6) Gaffuri, A., et al. Toxoplasma gondii in wild boar and roe deer in Northern Italy: serosurvey and PCR-RFLP. 9th Ewda Conference, 2010.(7) Renzi, M. et al. Serological investigation on the spread of Toxoplasma gondii in roe deer (Capreolus capreolus) Emilia-Romagna. Poster presented at the 3rd National SIEF Congress, Torino, 2009.Dogs and cats(8) Scarpulla, M. et al. Comparison of indirect immunofluorescence and ID Screen® Toxoplasmosis indirect ELISA for the detection of antibodies against Toxoplasma gondii in cat and dog sera. Poster presented at the WAVLD meeting, Madrid, 2009.
Wild & domestic animals(9) Roqueplo, C. et al. Toxoplasma gondii in wild and domestic animals from New Caledonia. Parasite, 2011, 18, 345-348.
91
Product code TRICHIS-MS-2P
Kit format 2 plates
Reactions 192
Plate format 12 x 8-well strips
TRiCHiNELLoSiS
Indirect multi-species ELISA kit for the detection of anti-Trichinella antibodies (T. spiralis, pseudospiralis, britovi and nativa) in serum, plasma or meat juice from pigs, wild boar and horses. Please contact IDvet for use with other species.
ID Screen® Trichinella Indirect Multi-species
Associated Products
Method Indirect ELISA
Species Multiple species, including pigs , wild boar, horses and others
Specimens Serum, plasma or meat juice
Coated antigen Trichinella E/S antigen
Conjugate Anti-multi-species-HRP conjugate (concentrated 10X)
Specifications
Ordering information
Highly sensitive and specific
Applicable to multiple species, including pigs, wild boars and horses
Serum, plasma and meat juice protocols
Trichinellosis positive freeze-dried serumProduct Code MRI-TRICHI
Format 1 ml vial (freeze-dried)
Description Freeze-dried positive porcine (wild boar) serum to be used as internal reference material for quality control.
Trichinellosis negative freeze-dried serumProduct Code MRINEG-TRICHI
Format 1 ml vial (freeze-dried)
Description Freeze-dried negative porcine (wild boar) serum to be used as internal reference material for quality control.
92
93
ComPANioN ANimALS
94
ComPANioN ANimALS
Method Indirect ELISA
Species Dogs
Specimens Serum and plasma
Coated antigen L. infantum purified antigen
Conjugate Anti-canine IgG–HRP conjugate (concentrated 10X)
Product code LEISHS-2P
Kit format 2 plates
Reactions 192
Plate format 12 x 8-well strips
CANiNE LEiSHmANiASiS
Indirect ELISA kit for the detection of anti-Leishmania infantum antibodies in canine serum and plasma.
ID Screen® Leishmaniasis Indirect
Specifications
Ordering information
Particularly rapid, notably in comparison with indirect immunofluorescence
Improved specificity thanks to the use of a L. infantum purified antigen
Easy-to-use, with results in 90 minutes
Associated Products
Product Code IFI-LEISH
Format 10 slides, 10 reactions per slide.
Description Slides for the serodiagnosis of Leishmania by indirect immunofluorescence (IIF) in dog sera.
Leishmania infantum Immunofluorescent Slides
Leishmania infantum positive freeze-dried serumProduct Code MRI-LEISH
Format 1 ml vial (freeze-dried)
Description Freeze-dried, highly-positive serum from a naturally-infected dog.
Produces a positive reaction when analysed by ELISA or by IFAT (Indirect Fluorescent Antibody Test)
To be used as internal reference material for quality control.
Leishmania infantum negative freeze-dried serumProduct Code MRINEG-LEISH
Format 2 ml vial (freeze-dried)
Description Freeze-dried negative canine serum.
Produces a negative reaction when analysed by ELISA or by IFAT (Indirect Fluorescent Antibody Test)
To be used as internal reference material for quality control.
95
ALL SPECiES
96
ALL SPECiES
ComPLEmENT FixATioN TEST
Guinea pig complement
Veronal buffer
Product Code CPLT-2x5ML
Format 2 x 5 ml vials
Product Code VB-4L
Format 4 vials (powder form). Reconstitute each vial with 1L of distilled water
Freeze-dried guinea pig complement to be used in any
complement fixation test (CFT)
Once reconstituted, the complement is stable for two
weeks at 4°C without loss of activity.
The complement system is a system of serum proteins that react with antigen-antibody complexes. If this reaction occurs on a cell surface, it will result in the formation of trans-membrane pores and therefore destruction of the cell.This characteristic is used in the complement fixation test (CFT) to detect antibodies against infectious animal diseases.
The complement fixation test requires complementary reagents that are not included in the kit. (Calcium Magnesium veronal buffer, haemolysin, sheep red blood cells, and specific antigen for CFT).
The Veronal Calcium Magnesium buffer is used to dilute
sera and reagents for the complement fixation test.
Once reconstituted, the veronal buffer is stable for 2
months at 2 – 8°C without loss of activity.
97
Fully integrated disease management system Determine titres, vaccination age and baseline values for better preventative medicine for poultry.
Easily share your results with others OD results may be exported to other formats, such as Excel.
Optimize lab time and plan your testing sche-dule
The software provides you with an overview of the different tests to be run for each sample.
Flexible Collect data and edit reports from multiple work stations.
Customize your templates and reports, and incorporate your own company information and logo.
Easy access IDSoft™ may be downloaded from the IDvet website – no discs are required.
1) Download ID Soft™ from the IDvet website: www.id-vet.com/support/software.
2) Contact [email protected] to obtain the User Guide and your personal login and password.
3) Use your login and password to download kits to your repertory or to install updates as they become available.
DATA ANALySiS PRoGRAm
ID Soft™ is a fully integrated, easy-to-use software program providing convenient, flexible and comprehensive data analysis for ID Screen® ELISA kits.
ID Soft™
Features
Installing ID Soft™
TECAN Sunrise
MOLECULAR DEVICES EMax
BIOTEK
EL311
EL800
EL808
ELx800
ELx808
Reader compatibility
Microsoft Windows 2000, XP, Vista, Windows 7, Windows 8
50 MB of free hard-disk space required
Internet Connection
Minimum system requirements
98
* Contain 0.03% Merthiolate as a preservative** IDvet product code or technique
Disease Status Type Species Volume Product code For use with**
RUMINANTS
AKABANE Positive Serum Sheep 1 ml MRI-AKA AKAC
BESNOITIOSIS Positive Serum Cattle 1 ml MRI-BSNT BSNTS
BHV-4 Positive Serum Catlle 0.5 ml MRI-BHV4 BHV4S
BLUETONGUE Positive Serum Cattle 1 ml MRI-BTC BTC, BTED
Positive Milk Cattle 1 ml MRI-BTMILK BTSMILK
BLV Positive Serum Cattle 1 ml MRI-BLV BLVC, BLVS
BRUCELLOSIS Positive Serum Cattle 1 ml MRI-BRU BRUS-MS, Rose Bengal, CFT, SAW
Negative Serum Cattle 1 ml MRINEG-BRU BRUS-MS, Rose Bengal, CFT
Positive Milk Cattle COMING SOON
BVD/BD/MD Positive Serum Cattle 1 ml MRI-BVD BVDOS, BVDC
Positive Milk Cattle COMING SOON
CHLAMYDOPHILA ABORTUS Positive Serum Sheep 1 ml MRI-CHLM CHLMS-MS
IBR Positive Serum Cattle 1 ml MRI-IBR IBRS, IBRGBC, IBRGEC
Positive Milk Cattle COMING SOON
INTERFERON GAMMA
(NATIVE)
Positive Plasma Cattle 0.5 ml MRI-IFNG-B IFNG
Positive Plasma Sheep 0.5 ml MRI-IFNG-O IFNG
Positive Plasma Goat 0.5 ml MRI-IFNG-C IFNG
Positive Plasma Buffalo COMING SOON
MVV / CAEV Positive Serum Sheep and Goat 0.5 ml MRI-VISNA/CAEV VISNAS/VISNAB
NEOSPORA CANINUM Positive Serum Cattle 0.5 ml MRI-NC NCS-MS
PARATUBERCULOSIS Positive Serum Cattle 1 ml MRI-PARA PARAS, PARAB
Negative Serum Cattle 2 ml MRINEG-PARA PARAS, PARAB
PPR Positive Serum Goat 1 ml MRI-PPR PPRC
Positive Antigen - 1 ml MRI-PPRAG PPRAG
Q FEVER Positive Serum Cattle 1 ml MRI-FQ FQS-MS
RIFT VALLEY FEVER IgG-positive Serum Cattle 1 ml MRI-RIFT RIFTC
IgM-positive Serum Cattle 0.5 ml MRI-RIFTIGM RIFTIGM
SCHMALLENBERG VIRUS Positive Serum Cattle 0.5 ml MRI-SBV SBVC, SBVS, SBVB
NEGATIVE BOVINE SERUM Negative Serum Cattle 0.5 ml MRINEG-BOV ID Screen® kits for Aka, BLV, Bru, BTV, IBR, Paratb, NC, SBV
AVIANIBD Positive Serum Chicken 0.5 ml MRI-IBDS IBDS
Positive Serum Chicken 0.5 ml MRI-IBDVP2 IBDVP2
IBV Positive Serum Chicken 0.25 ml MRI-IBV IBVS, IBVC
ILT Positive Serum Chicken 0.5 ml MRI-ILT ILTS
INFLUENZA A Positive Serum Chicken 1 ml MRI-FLUACA/N1 FLUACA, FLUACN1
Positive Serum Chicken 0.5 ml MRI-FLUACH7 FLUACH7
Positive and negative freeze-dried sera and milk to be used as internal reference material for quality control*
ALL SPECiES
99
Disease Status Type Species Volume Product code For use with*
MYCOPLASMA
GALLISEPTICUMPositive Serum Chicken 0.5 ml MRI-MG MG/MS, MG, RSA-MG
MYCOPLASMA
MELEAGRIDISPositive Serum Turkey 1 ml MRI-MM RSA-MM
MYCOPLASMA SYNOVIAE Positive Serum Chicken 1 ml MRI-MS MG/MS, MS, RSA-MS
NDV Positive Serum Chicken 0.5 ml MRI-NDV NDVC, NDVS
SALMONELLA B & D GROUPS Positive Serum Chicken 0.5 ml MRI-SALS SALS
NEGATIVE SPF CHICKEN
SERUM
Negative Serum Chicken 0.5 ml MRINEG-BIRD ID Screen® ELISAs for avian
diseases
EQUINE
EIA Positive Serum Horse 1 ml MRI-EIA EIAED
EVA Positive Serum Horse 0.5 ml MRI-EVA EVAB
STRANGLES Positive Serum Horse 0.5 ml MRI-STREPTO STREPTOS
WEST NILE IgG-positive Serum Horse 1 ml MRI-WN WNC
IgM-positive Serum Horse 0.5 ml MRI-WNIGM WNIGM
PORCINE
AFRICAN SWINE FEVER Positive Serum Pig 0.5 ml MRI-ASF ASFB
APP Negative Serum Pig 0.5 ml MRINEG-APP ID Screen® APP ELISAs
Positive Serum Pig 0.25 ml MRI-APP-2 APPS2
Positive Serum Pig 0.25 ml MRI-APP-1-9-11 APPS1-9-11
Positive Serum Pig 0.25 ml MRI-APP-4-7 APPS4-7
Positive Serum Pig 1 ml MRI-APP-3-8 APPS3-8
Positive Serum Pig 0.25 ml MRI-APP-5 APPS5
Positive Serum Pig 1 ml MRI-APP-6 APPS6
Positive Serum Pig 0.25 ml MRI-APP-10 APPS10
Positive Serum Pig 0.25 ml MRI-APP-12 APPS12
AUJESZKY’S DISEASE Positive Serum Pig (vaccinated) 1 ml MRI-AUJGB AUJESZKYGB
Positive Serum Pig (infected) 1 ml MRI-AUJGE AUJESZKYGE
CSF Positive Serum Pig 1 ml MRI-CSFE2 CSFE2C
HEV Positive Serum Pig 1 ml MRI-HEV HEVB-MS
MYCOBACTERIUM AVIUM Positive Serum Pig 1 ml MRI-MA MA-MS
MYCOPLASMA
HYOPNEUMONIAE
Positive Serum Pig 1 ml MRI-MHYOP MHYOPS
SVD Positive Serum Pig 0.5 ml MRI-SVD SVDC
TOXOPLASMOSIS*** Positive Serum Pig 1 ml MRI-TOXO TOXOS-MS
TRICHINELLOSIS Positive Serum Pig 1 ml MRI-TRICHI TRICHIS-MS
Negative Serum Pig 1 ml MRINEG-TRICHI TRICHIS-MS
COMPANION ANIMALS
CANINE LEISHMANIASIS Positive Serum Dog 1 ml MRI-LEISH LEISHS, IFI-LEISH
Negative Serum Dog 2 ml MRINEG-LEISH LEISHS, IFI-LEISH
*** Note: The negative APP serum (MRINEG-APP) is also negative for Toxoplasmosis.
310 rue Louis Pasteur, 34790 Grabels - FRANCE
Phone + 33 (0) 4 67 41 49 33Fax + 33 (0) 4 67 45 36 95
[email protected] www.id-vet.com
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