bull

Extremophiles These microbes thrive zmder corditioflS ha

would kil other creatures The moecu les Ihot enable extremophiles to prosper

aTC becoming useful ta industry

- 1=Il diUlIlnlO bull rr~

cool immi~ pltlOl Nltgtw INnk qud of plowtnc inIo lff dur

IJ boillaquo lIeU ampCUlIWo OranMln jwnfJirl in Drp~~ ~mshy

la Of ClIlllaquoIlIrIlN brino Tht IclIp OOIl dilasfro for bull pnoJD Yet mmr~n makw in sudl btlildrt mirombullbull nc minot In albJ ~lo t shycgt rhq rhn ~COlldocircrialSm rom Ibo h arc crly lX

tr=Ilt Amuintll thlt OfFn do mr~ lbrir lhlty do 11lt0 id rholr PUIII~ bitnd IR 1

ltlI Iftl UfrtmoS in ordtflO~~Id

Somr G1Ii ho brm kmwa for 40 ~I$ lIIll dtlt ltdI Ihltm hagt 1mUIiltd rllt Iumngruud m_n ~ orn bull j 10 br 0lUiIt ~bltM ith mimiddot cn1lU1 61 n buno tu ho boa lumiddot dnl doc Pf 1fUt br iMuf IJY) ruliz rIut thlt - 1kbull ~~~ltlllpotnl

uuy _ in ruycipp1anonbullbull Ofpaniltular inl=M me mzfmtO

(bOoL 01 ltIllgt1r-gt1 du hqgt ltXba

piles 10 tuncnollll bnPl tirtumItI n likc f1lthlttic clIltllfgtO ~mn

h an l ltperd up chom 1ilittlolll bol lfefnl tilt ton ~-r hkwiampdIIirIdDIl odwr lndIlllM OltIdwidt 1tI llOft lIun SU 1gtilI ltntymltS F appli a rllllllirom rht lnd ni alOi onltI~- 1O m FfC odm- of cnnurulo one dw dii of rftmouo onlt pshynltric ~ Yltt gtlttnlthrd mI)Cgt

tm whm ~ 10 h or ltJI gtol nuNmgt rdv nn doom cim ltpr lm ro plaquonlt1 dw lftM do molt Of Il Br 4IKshynv CltttlJI 11 foo~ ymos fnIm mdOlllllol obbN ~clturmiddotmltI ~-om putontulv mm_ dw v b mr addoJ

sCopJn m

mmy~dicicny oncl rnNcshyIumllI- Thoyaonhobmm of mlirdy new lttlyrM-blIlold jICOCltgtW$

~2(I~pwpc us Jpn GltrmocIy oad ~ _ ny 1iCI li nhltlifliob and cllltit ~Ah~ only bull iltw D_ l~rmegt hJ bullbull modo hrif _ inru If flr ~ 1ltgt io ttW ofIWJducl_rmtt lnIrlliocmmiddot a _Iy Id1ld ~ inrn bIe p lior can h SCnl aD

SoucI of Oh hltgthrlronl mlnw dw MlIuIiuull lm of ~rc N mil do hdd ~ CtIlgtld imo WtI ho-oie lateril timplemlo Iodt nicw and Yo odIo complu The _ wotIrltnd Il n 1pregtshy1m 1 ft rhonI mm- ltXDflt ANIIybullbulln ladlt bull IoIcIy bilt ~nothltt NId m rdIM1 pnn hJ ufI holnia ft 5lII dw PfO Iunrnoa

PlINlSI-lLlG fNVIRONMINT5 1ft bull ~ n ht-r-d 1000lt_ dtpicted

H middot ~middot~ phi III -Dl - ondiod

ol utraIIogtpIugraveln l1Mml gtoJ ur tUdilr Iollmlpmmiddot mres ~a mll 4$ cnw II Il dovltn Flvmhm and ltli rhrm t=d hl~ ro lomIp--3- 10 dosr- C (116 q FI Sltwnr h~ ph ~- lhm Il 11_ 100bull ln Iwo 100 ltleyeltr CIll drrHt f 1Iw hagrave-l POO nl ~ ~bulleo ri III pgtrioœ prdm]Inlttv hamshyrU puw fau in lUffJ -m 1J and lt10 ltltlIren C (77 -J 104 ~ Ft r~ no mllulJ 1gt pLul h~C hmI foonJ 0 NImiddot 3~ rltrm-n ~hobullbullbullbu sa LIemiddot c (lU sr- flbullbullnd _ bul fi d~-I Cln roIn-

bull rm 1lfIampU m remrrnNlllJher dwI bout 6(1 dqren C (HO ~awenI penlWD fi 10 6(1 LIepeo C hu-n a- b bur plusmn 10 lwmtI in aream hul-wftlt lino d ~ _boat JO JU n D Idlt motltd ampc l1nIlt-a of IV _~- op 1__ ml m fPlaquogt_ ltJuriaC bull ~-ltcna -=rob1 61on hot ~ oi NoaonoI Pul W~

no __p iDuDd 1Iw _

- PU1ed Iik III 1 196Oo liwy drnnMdhe for ~aplblr ofYOolW~ mpertumo JlegtaMa ro~Cb_J-1n__

Itd TMnltl dlgtI wouW btct mak _ibIe iltItopKld ltli 3 rr ord (ltIlo met dIol gt(lgtlft (PCItl buuc lbagt me Ilme thlt am kgtUDIi rhltint hy 10 Il D Il hound ltOdic_ l1ul 4 dwnhgtemiddot

CopyrlaIll lm Sdonllllt uMrilt

0 sJ(uIuln tUltinu IW pllfiaRy bullbull ptmNfft -1IiP _ as ~ c n ~ 1 anI con ht pr-ricircn boiIinf shy

Iltodlt (0Il(1IJeJ rom lIw (urin ~ Iueterio funcrion II

JNhelt ptUlU hao ovlcol andpowJlhor~_kl

lilltdr bo id ~ter ~ Otheo 11 iltduJIcircolI h thoo __ m ure 1971llt k ICishy_ fO mon J on -J hyIrothnshy1 -m lIIOnS 10 H iliiIl_ ctIcd -_ 1Ilaquol1 dmn radlt cNnwyo ltwuIh 1UdI mopI pedlnlfd aoirInraquorido lluod bullbull hot )JO-c

10- mon +0 saae al hy pmhomaogtphib lu hem -wd -r~O s-n cUshy~ mUao-yal~

~~~-= 11 Icircltl rhc h al Ir rqgtOOshy

SclDltTlIlC AgtoUICUi AIriII l

bull

duca loe Ih ltJi oOouf lOf ~CIaJ1 nllpIy __ p al P 1U dcpel C Ileshy~IcircI PO lf_bull t1ftI bdow 10 ~C (lM shyf1 Il ltllI1d1 -t-Ioypathctshyophie lNllmJ dup tel dlim~ fiIo _ -pInriclaquoœ-o M 0001) _ dro mld1 ana Iiooo il litI dot fOOIl il the of Iiir anaIyoio ni aod Iioilif if UpenN 10 btlp dariIr how

Noe Too Hoc III H-IIo

R-- 1Cfed fiIo -bOre oi 1 IcircIlI shy

IcircII rirr _ ff71IlIlOdrncand how in Iorinamp -roba lld odoltr umpu io fwlcoOlUI r - lNbull odoolOd biolP bull~ ~

_pcnrr CUumlIHL 1111 il shydolt _ft o1dlouch IcircI _ _

~I diHu -l Ir dn For lltUlIIl bUl-1ooonIIl afnlll shy0I1fIle$ raaDbk lthoir beo-ocircoodonnl

dw _ O J _I-a 1r1Ugraveu middotoct io -- ~cl)lkal nIo il -IimI fi (- bull for HJV ~Rd~ - ru FOcirc OIOItIepacircbility 10 _____bull 1-shy shy

In lC1 - DNA poIltUJIOcirclIlur1onipptt01 DNA prodllQ 0__ p p)l Tho _011 roiaor 10 lIecyccd Ioorwcco lowarwl _W1IaoMshylion iol -l ~ohcpoty-- _ Ire- aobct tIlIf bullbulln ROC l Licnd 00 0f01lltd kmiddot

lbc Iwx plll 01 plcnd~bull Tlaquohnitwu hod rplmilh lhe en-IlMI -urIm le

To pcJ 11lQl oacnrion ecn plodcd 1 f shyampcm dculqhouoc wllen Ilrock kad dcpooiled pIa oupIy 10 fCIIlt lier The iIIlon Theft iooLotcd doo= m~ DNA poI lT poIr

Copytlplt llln $dfUIc ~l F

-1 1 ~ Ile for 11 IN lu 101 oIlUJIlly 0Uf0m2I

cd rat ~b-m rIOCDC of PCll ho roploced dw T~

r-gtln- idI 1ftlt pol Th cnqmc ioulMed Icircfltlm die hypcnhlaquoshymolloiIf lroct (degflmi lircWIj Itlt -1 100 C

Adom-bc commcnioJ ~ fi linrtIcy w ~ allN C(~IR procMood fnIm tch CydoMlrxtrino ~ 10 lfabiliK talCultoIIo io frlnlthl In P doc ll4lab 01 mrdishyciDn br tIc body and hl -_ shy

odon onalitinet

cow an

_nr __Iho -lIr-shybodl - oI_mm drpaC(w JI F) a 0- Mlf lM oanhampco AoIlIIl1oftd __ cl dw Nnic lOlo Mshy1fUio pl wly dor for sshyp )r- plloshyn likc rhe tlorlfIf PPG Iik oh - oi MlthroplWoo (ltDld Iowcn~

Ju- T Sulqo ud Uuml -

~t=~ obioJ=-me lfUlI AntllClic bgtshyocean bullbullIf lhaI ffllllino froun for mudl cl yult TbrocCCOlll1WllIcirctico lashydudo uumldilcrilt karyabIJ alpe od dlofOllIS Il wdI Il bull ftlircy of borio 0 bolttnillbUMmiddot 1br Sulqo 1fOIIP oJ-p___dnmiddot io plinu ltpfe5ltnlgtti 01 PJIChro pIiIo oprisul prnnue lor -m io C od io fiumlogtdI -puashybom Il dtpeaCfOO kir uproclucocircoL CIl kbc OfF bull Iflllal fO im lUcircoawm who nccrl CDIJIbI dool rfriImiddot lIfQlltonar-- faocl __ 1-- produca uquifc w _~ ~

aukm oIlnampn_lhidl po

Il ~ ~11JtCl 01 Imiddotwbullbull tclry WPB A- exvemopQiln DO

uuml ll

bull

7 c en c Anlrri

1

) _ ln__

tItIIdtIls Ihtllm riI INad__ itIArchatll Makn Thrlaquo ___edlnlUl

no_ odo lIpoœit1lo Ill shy rill1oove ~-cl --Ss _ril il =0 i bull oIlltf1tlo wldl ft bull oM Il 4~_M -9ftft _ lot

Il 0-_ shy__-ftlIdNIl= r ------- = 7 1 11~ _111

_ ~ Fon) sJi t bullbull1 r U1 __0_ _ CIII_as__e- ~ bach _ _ lht I _ ~ d Itlt ~

--M__ ri 1 IS 1 d__01 100- =

t ~ bullbull 1Il~- shy lIcoi Noct ____No

lht s _ -__ _~_ --_---1

1CMn_ bull _

10 ___

0001( bull ~ 1J77 _ bull s _AJlIlN~ _~l _4 _dao1lo 1- _- -Mrt6shyIl -cuIwi tIUgraveMbIC-

Il _ m-Il~

na toi oH pblt fO fcK ~ nxIE III fonz Ullft IIgt dit llbIy foaMd ~ 71w fatiilalCl r ltIiopnIioIl encbl pupo ~ lItIon UcircIc oil WI pat

tc===4r$r IcircI tddaI jgoc bdo - jomI dolt shy1Ind _ Tf ca _ Ibo LfeOshy

liF - _ J ~ Aa bull 1 rio tJ __0 middotd ---a ~œbull Rr- -- ~ iwc S ncILIioiot __ -M

st _ca

w IId - tmiddot II _ = ~ loItroiNunli s Uoi ~

Il -u_)_~IcltoIc_

middotlJlllshy_ jIftIiNo woooW _--

F- Jt-o 1 e--tIodl _ 01 DN- J IU) ~ a - am_ _ az AI _ J - shy ro- __bull __ 01 cu bull 5 -~ -b _ nr ~ bull DNA 4a ad_

f T_~iuml+

bull fi n ldi-l- po- ltuhm 01 na rn u -If _ IR Iookont fol M ~

wli __

t Pt ho

11 7lltooblc

~DNA _~ 1 1 11_ doc 1 01 _ 1

loouumlo bull

-0 tz -d i1ioolI ~

-m t~iI~ 1 Icirc1idoou fi It_ bull dG) 100 = _alf

1CtdiIy aiochal_ Md ~ ~d r~~ ~ docl ---

bull i ~ J a 1ctW wlioltDfd Olt Il ad lLCiicy if ole 1 _hen 10 otl hnicoJ_ _ ~ mpoMlWo kir cho_ Doriry _g ha lmiddot_ _ doc LU lied rift iD oloocr M JUIcircOI doshy

bull ot in S

~bull--r CAO S 11lq dot DNA rr-~ _ -001 -wl n-n exshy_ao bullrn-_ __ DNA -1 u~

--li bull - shy -a_ _ li COlI i _

__ rilll _ shy IlIIHwI

Mt

S - -

~1997k~IIt_ -

~ altcNCfIn_~lt-flIlhIt_b1IliI ~(Mlbl_lollhe _1) IIH ioMlt ~ II aphlic OIll9flMII$lIM 9 _ Mon fA lIMue nNNl9tfle$ ~

_ - 1IIri Insighu lnta _ 1Ulttgt 1Ifo- pl 10laquo_1 JqJiIo IboocWrioL d cd ~ œnoIn ail _ -anboonollclf ~IDfODC SdiwIIlIU OW ID n IN _ bIrbo8OislJdbdtcI~9In__bullbull 0I1O

u ID _ Gmn bIutpmu ifonI _ PIfbm -11lt1 induumllt __rltlIldampul ail he-N

- 1

i 1 1 1

1 hdp tIIZJGlel from onlimrp rnicngtba inm anilicial U1twfId

DiIrofflY cl~OC- 0jlfkW MImiddotmiddot br_de 1 i aI au_dM _ pobiluumla Y for lIeOI cuyme lU pin ~=pta-itomair ilI lia~ 10 kttp down Ihc (Otef 0 pt(gtshy

dacrion m~~~tIw -1 xm_r= prodllCilll tamporr will rdUbly mm al PfOI Tho dtfkpllilaquo of ~ ufKlUriaa trdIniquco and thc le-

Illqanceol iod Wltwe JJQ tl Uead e-bIr wd0llUId ~lItUJof -u (CiIlIa li _ ilwgtillb ~

or tbcilt _r aIP na nad proote irrnioriblo c

pm PfOIiUI Degtoota 01 he wshyet ~ known dirtard cYltgtlushy

OIlOl froaI

bon k Of 1 rudom nashyna the ~~al-

~ llW acnerthoo do of diffnm of the enzynw T1wn Ihey 0ClfII0l1hc shylU hnhee of lhe bullbullriItioGo pintd topod-b futurlt- Th Lui IUllteV il aloo Wd 10 he PdioonLon co ncn Ed -pt bull -loI 10 u bull 6ItmmT br doc liahlbtlb he lriod ~ ilable m bombou 10 oiIIr ttado nd dlott br_I WOIked bat

iCI far _ ntnlOltf1OtO in shyaM lime olœrod choir iampioal Mm n raQoltlaJ dollp NId Edooniaa ppruiOCha prumi la

halllaquo emlII(Iamp)lIl Tbey aloo

Loi

_____

-ER

Extremophiles as a source for novel enzymes Bertus van den BlXg

M_ lObe_ shy on bull _

bo_~ __ _ ft _--lI)r_oI_pH TI-__ _0 No IIogtOIIbool lnl

Cono po _al___ --10__ ~bull Ol_~ PlOC N __ 0l

J= bull _ EMII__ i __

1 __plt _ W_do bullbull_ - 01 _11__ _ - =ozrno _ __ lol 01 0 -pl _ _- r

_l _ Il4tIo_lIIllO_ _ Tho ----_---___ ____ la _11

1Ol __---shy~ 00 br l-lq lodulriIoI dc d fOI bioatayJ do lM COI _ cooodiltions id obIlt cfllwto ~ Nu -od co lh ~ rOI _h uyma cmoaI arpalc tloaio bIcircolIc31alyI emn lM boette cheinl pl oohilth con kod 10 _ cl5acircelo pnodian 01 10 gttltragtIumlODltIgtltB (cr bull tt iffNIrtlltlllll nkn Il ~ ~ilC Ibc ampa do ID do _ dun JOOO dlll_ euyma lM oori6ed or thcfCl dleir-~ iDdtauiol pIi_ he pracA UZ)1IM bon niU _ 1Iccediloe bull de A0a_ dl iam foct _y Iob euyma lN _ wilttltond ml ~

-Ji As rauk dM lt~ iu almia Ole ~ i~_C1I famp_ hot igt pcoc dcugrave oudo zac-phila gtIe _ cl ~1U 1~J~

Eure QOnd fCfc 10 pII)l coIre le _pc Je ndirlti_ bult ID _

_ u1gt Ii~ad pH (rhI Il loi vt doc couemovtoilco h bec idltn~d Olle bclonJ 0 1 01 Ile AIclI bull _bullbull bullbullny nunoophilc r dgtc ria ond uy roc i bullbull o Illy ItIcntlllcd od dlancuriud

TIoc _ion ilco C090ble rviY uncla no eootchfoollt __lion1 shy_nu h Icd _ doc _nies Mw _ becn Yod roc_ oondm- 1 d bull -idaob6c r do t do hbullbull hcgtIotood d IIlncucircofldy cboruotiocd r- lU pH bull HP _ lIcircIIn

1 ~ l aDd diocua 0CCCm pl bull shydlo d-IJ iIoNoliobullbullnd ~ 01 ~ r _pt-_shyAi illu cd ln Tobie 1 lM el licol COllall numiddot lcr widc iEry d~ middot diliom Iugravech pnilm odoptcd nc b Iyo i_ m- doc =p cauld bclBmiddot

IIIlpliablc ibltty dio-ne fToble 1) F do dep cL po)mcn dli _ bull _ r- dobullbull _ aetiw bull and - 10 ltiIlt ~nfCIl ohe prcfcrrcd Us- the _ lb ooIwl Ugtd __tIy oIIc litlt libiliq tbc boltnf p_ AltanoIJ ir lgtftC necbullbull pcltshyfoc RCf_peooilk odIl1cUcircltXI 0( ltOnfIOOlnd lof tbc lDdlais cL bull phomuautioaIly le pouduco Ofpnilt oohc IJ diIf nl ~i ply bull the 1Hoo1you AI II Icirci rclthcc --- ocorilty tIZflH m- loIcgtpIlilif le OIIOIld bc _ ibIc clioicc r pptaiDn i__-dia 1~5I n Ji ~ ofn i_mc lOI ~ di ltIfICplile c dopltcd off yaciriof fIlin rOf 0 ~riuy 01 oppli(otiofto

_ hM TIocpliilt CJIrcmophilco ~ cd _ ne rion kul umophlllc ptOIlt_ li poIy-dqndil lt110) IUCh n dlul h bull d yI ~Vll rotlCl lb IAlI Inod il _10 IThIc 1~ Tho fCO-o 1)1

Otobk nd oeil ol ~cd PfM- obtmiddot A icnd nou he oolubilify 01 noy ion laquogtngtpOfInll in penk ltr po)merlc oubouo bull liancly cd MOfco~ dobullbullioIr 01 11oft ludinl 0 undctifld 00000pliulOf 1 uh ed hipMr

_ fin _ _ _ Il -1

bull

PlINlSI-lLlG fNVIRONMINT5 1ft bull ~ n ht-r-d 1000lt_ dtpicted

H middot ~middot~ phi III -Dl - ondiod

ol utraIIogtpIugraveln l1Mml gtoJ ur tUdilr Iollmlpmmiddot mres ~a mll 4$ cnw II Il dovltn Flvmhm and ltli rhrm t=d hl~ ro lomIp--3- 10 dosr- C (116 q FI Sltwnr h~ ph ~- lhm Il 11_ 100bull ln Iwo 100 ltleyeltr CIll drrHt f 1Iw hagrave-l POO nl ~ ~bulleo ri III pgtrioœ prdm]Inlttv hamshyrU puw fau in lUffJ -m 1J and lt10 ltltlIren C (77 -J 104 ~ Ft r~ no mllulJ 1gt pLul h~C hmI foonJ 0 NImiddot 3~ rltrm-n ~hobullbullbullbu sa LIemiddot c (lU sr- flbullbullnd _ bul fi d~-I Cln roIn-

bull rm 1lfIampU m remrrnNlllJher dwI bout 6(1 dqren C (HO ~awenI penlWD fi 10 6(1 LIepeo C hu-n a- b bur plusmn 10 lwmtI in aream hul-wftlt lino d ~ _boat JO JU n D Idlt motltd ampc l1nIlt-a of IV _~- op 1__ ml m fPlaquogt_ ltJuriaC bull ~-ltcna -=rob1 61on hot ~ oi NoaonoI Pul W~

no __p iDuDd 1Iw _

- PU1ed Iik III 1 196Oo liwy drnnMdhe for ~aplblr ofYOolW~ mpertumo JlegtaMa ro~Cb_J-1n__

Itd TMnltl dlgtI wouW btct mak _ibIe iltItopKld ltli 3 rr ord (ltIlo met dIol gt(lgtlft (PCItl buuc lbagt me Ilme thlt am kgtUDIi rhltint hy 10 Il D Il hound ltOdic_ l1ul 4 dwnhgtemiddot

CopyrlaIll lm Sdonllllt uMrilt

0 sJ(uIuln tUltinu IW pllfiaRy bullbull ptmNfft -1IiP _ as ~ c n ~ 1 anI con ht pr-ricircn boiIinf shy

Iltodlt (0Il(1IJeJ rom lIw (urin ~ Iueterio funcrion II

JNhelt ptUlU hao ovlcol andpowJlhor~_kl

lilltdr bo id ~ter ~ Otheo 11 iltduJIcircolI h thoo __ m ure 1971llt k ICishy_ fO mon J on -J hyIrothnshy1 -m lIIOnS 10 H iliiIl_ ctIcd -_ 1Ilaquol1 dmn radlt cNnwyo ltwuIh 1UdI mopI pedlnlfd aoirInraquorido lluod bullbull hot )JO-c

10- mon +0 saae al hy pmhomaogtphib lu hem -wd -r~O s-n cUshy~ mUao-yal~

~~~-= 11 Icircltl rhc h al Ir rqgtOOshy

SclDltTlIlC AgtoUICUi AIriII l

bull

duca loe Ih ltJi oOouf lOf ~CIaJ1 nllpIy __ p al P 1U dcpel C Ileshy~IcircI PO lf_bull t1ftI bdow 10 ~C (lM shyf1 Il ltllI1d1 -t-Ioypathctshyophie lNllmJ dup tel dlim~ fiIo _ -pInriclaquoœ-o M 0001) _ dro mld1 ana Iiooo il litI dot fOOIl il the of Iiir anaIyoio ni aod Iioilif if UpenN 10 btlp dariIr how

Noe Too Hoc III H-IIo

R-- 1Cfed fiIo -bOre oi 1 IcircIlI shy

IcircII rirr _ ff71IlIlOdrncand how in Iorinamp -roba lld odoltr umpu io fwlcoOlUI r - lNbull odoolOd biolP bull~ ~

_pcnrr CUumlIHL 1111 il shydolt _ft o1dlouch IcircI _ _

~I diHu -l Ir dn For lltUlIIl bUl-1ooonIIl afnlll shy0I1fIle$ raaDbk lthoir beo-ocircoodonnl

dw _ O J _I-a 1r1Ugraveu middotoct io -- ~cl)lkal nIo il -IimI fi (- bull for HJV ~Rd~ - ru FOcirc OIOItIepacircbility 10 _____bull 1-shy shy

In lC1 - DNA poIltUJIOcirclIlur1onipptt01 DNA prodllQ 0__ p p)l Tho _011 roiaor 10 lIecyccd Ioorwcco lowarwl _W1IaoMshylion iol -l ~ohcpoty-- _ Ire- aobct tIlIf bullbulln ROC l Licnd 00 0f01lltd kmiddot

lbc Iwx plll 01 plcnd~bull Tlaquohnitwu hod rplmilh lhe en-IlMI -urIm le

To pcJ 11lQl oacnrion ecn plodcd 1 f shyampcm dculqhouoc wllen Ilrock kad dcpooiled pIa oupIy 10 fCIIlt lier The iIIlon Theft iooLotcd doo= m~ DNA poI lT poIr

Copytlplt llln $dfUIc ~l F

-1 1 ~ Ile for 11 IN lu 101 oIlUJIlly 0Uf0m2I

cd rat ~b-m rIOCDC of PCll ho roploced dw T~

r-gtln- idI 1ftlt pol Th cnqmc ioulMed Icircfltlm die hypcnhlaquoshymolloiIf lroct (degflmi lircWIj Itlt -1 100 C

Adom-bc commcnioJ ~ fi linrtIcy w ~ allN C(~IR procMood fnIm tch CydoMlrxtrino ~ 10 lfabiliK talCultoIIo io frlnlthl In P doc ll4lab 01 mrdishyciDn br tIc body and hl -_ shy

odon onalitinet

cow an

_nr __Iho -lIr-shybodl - oI_mm drpaC(w JI F) a 0- Mlf lM oanhampco AoIlIIl1oftd __ cl dw Nnic lOlo Mshy1fUio pl wly dor for sshyp )r- plloshyn likc rhe tlorlfIf PPG Iik oh - oi MlthroplWoo (ltDld Iowcn~

Ju- T Sulqo ud Uuml -

~t=~ obioJ=-me lfUlI AntllClic bgtshyocean bullbullIf lhaI ffllllino froun for mudl cl yult TbrocCCOlll1WllIcirctico lashydudo uumldilcrilt karyabIJ alpe od dlofOllIS Il wdI Il bull ftlircy of borio 0 bolttnillbUMmiddot 1br Sulqo 1fOIIP oJ-p___dnmiddot io plinu ltpfe5ltnlgtti 01 PJIChro pIiIo oprisul prnnue lor -m io C od io fiumlogtdI -puashybom Il dtpeaCfOO kir uproclucocircoL CIl kbc OfF bull Iflllal fO im lUcircoawm who nccrl CDIJIbI dool rfriImiddot lIfQlltonar-- faocl __ 1-- produca uquifc w _~ ~

aukm oIlnampn_lhidl po

Il ~ ~11JtCl 01 Imiddotwbullbull tclry WPB A- exvemopQiln DO

uuml ll

bull

7 c en c Anlrri

1

) _ ln__

tItIIdtIls Ihtllm riI INad__ itIArchatll Makn Thrlaquo ___edlnlUl

no_ odo lIpoœit1lo Ill shy rill1oove ~-cl --Ss _ril il =0 i bull oIlltf1tlo wldl ft bull oM Il 4~_M -9ftft _ lot

Il 0-_ shy__-ftlIdNIl= r ------- = 7 1 11~ _111

_ ~ Fon) sJi t bullbull1 r U1 __0_ _ CIII_as__e- ~ bach _ _ lht I _ ~ d Itlt ~

--M__ ri 1 IS 1 d__01 100- =

t ~ bullbull 1Il~- shy lIcoi Noct ____No

lht s _ -__ _~_ --_---1

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10 ___

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Il _ m-Il~

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tc===4r$r IcircI tddaI jgoc bdo - jomI dolt shy1Ind _ Tf ca _ Ibo LfeOshy

liF - _ J ~ Aa bull 1 rio tJ __0 middotd ---a ~œbull Rr- -- ~ iwc S ncILIioiot __ -M

st _ca

w IId - tmiddot II _ = ~ loItroiNunli s Uoi ~

Il -u_)_~IcltoIc_

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f T_~iuml+

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wli __

t Pt ho

11 7lltooblc

~DNA _~ 1 1 11_ doc 1 01 _ 1

loouumlo bull

-0 tz -d i1ioolI ~

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1CtdiIy aiochal_ Md ~ ~d r~~ ~ docl ---

bull i ~ J a 1ctW wlioltDfd Olt Il ad lLCiicy if ole 1 _hen 10 otl hnicoJ_ _ ~ mpoMlWo kir cho_ Doriry _g ha lmiddot_ _ doc LU lied rift iD oloocr M JUIcircOI doshy

bull ot in S

~bull--r CAO S 11lq dot DNA rr-~ _ -001 -wl n-n exshy_ao bullrn-_ __ DNA -1 u~

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__ rilll _ shy IlIIHwI

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_ - 1IIri Insighu lnta _ 1Ulttgt 1Ifo- pl 10laquo_1 JqJiIo IboocWrioL d cd ~ œnoIn ail _ -anboonollclf ~IDfODC SdiwIIlIU OW ID n IN _ bIrbo8OislJdbdtcI~9In__bullbull 0I1O

u ID _ Gmn bIutpmu ifonI _ PIfbm -11lt1 induumllt __rltlIldampul ail he-N

- 1

i 1 1 1

1 hdp tIIZJGlel from onlimrp rnicngtba inm anilicial U1twfId

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dacrion m~~~tIw -1 xm_r= prodllCilll tamporr will rdUbly mm al PfOI Tho dtfkpllilaquo of ~ ufKlUriaa trdIniquco and thc le-

Illqanceol iod Wltwe JJQ tl Uead e-bIr wd0llUId ~lItUJof -u (CiIlIa li _ ilwgtillb ~

or tbcilt _r aIP na nad proote irrnioriblo c

pm PfOIiUI Degtoota 01 he wshyet ~ known dirtard cYltgtlushy

OIlOl froaI

bon k Of 1 rudom nashyna the ~~al-

~ llW acnerthoo do of diffnm of the enzynw T1wn Ihey 0ClfII0l1hc shylU hnhee of lhe bullbullriItioGo pintd topod-b futurlt- Th Lui IUllteV il aloo Wd 10 he PdioonLon co ncn Ed -pt bull -loI 10 u bull 6ItmmT br doc liahlbtlb he lriod ~ ilable m bombou 10 oiIIr ttado nd dlott br_I WOIked bat

iCI far _ ntnlOltf1OtO in shyaM lime olœrod choir iampioal Mm n raQoltlaJ dollp NId Edooniaa ppruiOCha prumi la

halllaquo emlII(Iamp)lIl Tbey aloo

Loi

_____

-ER

Extremophiles as a source for novel enzymes Bertus van den BlXg

M_ lObe_ shy on bull _

bo_~ __ _ ft _--lI)r_oI_pH TI-__ _0 No IIogtOIIbool lnl

Cono po _al___ --10__ ~bull Ol_~ PlOC N __ 0l

J= bull _ EMII__ i __

1 __plt _ W_do bullbull_ - 01 _11__ _ - =ozrno _ __ lol 01 0 -pl _ _- r

_l _ Il4tIo_lIIllO_ _ Tho ----_---___ ____ la _11

1Ol __---shy~ 00 br l-lq lodulriIoI dc d fOI bioatayJ do lM COI _ cooodiltions id obIlt cfllwto ~ Nu -od co lh ~ rOI _h uyma cmoaI arpalc tloaio bIcircolIc31alyI emn lM boette cheinl pl oohilth con kod 10 _ cl5acircelo pnodian 01 10 gttltragtIumlODltIgtltB (cr bull tt iffNIrtlltlllll nkn Il ~ ~ilC Ibc ampa do ID do _ dun JOOO dlll_ euyma lM oori6ed or thcfCl dleir-~ iDdtauiol pIi_ he pracA UZ)1IM bon niU _ 1Iccediloe bull de A0a_ dl iam foct _y Iob euyma lN _ wilttltond ml ~

-Ji As rauk dM lt~ iu almia Ole ~ i~_C1I famp_ hot igt pcoc dcugrave oudo zac-phila gtIe _ cl ~1U 1~J~

Eure QOnd fCfc 10 pII)l coIre le _pc Je ndirlti_ bult ID _

_ u1gt Ii~ad pH (rhI Il loi vt doc couemovtoilco h bec idltn~d Olle bclonJ 0 1 01 Ile AIclI bull _bullbull bullbullny nunoophilc r dgtc ria ond uy roc i bullbull o Illy ItIcntlllcd od dlancuriud

TIoc _ion ilco C090ble rviY uncla no eootchfoollt __lion1 shy_nu h Icd _ doc _nies Mw _ becn Yod roc_ oondm- 1 d bull -idaob6c r do t do hbullbull hcgtIotood d IIlncucircofldy cboruotiocd r- lU pH bull HP _ lIcircIIn

1 ~ l aDd diocua 0CCCm pl bull shydlo d-IJ iIoNoliobullbullnd ~ 01 ~ r _pt-_shyAi illu cd ln Tobie 1 lM el licol COllall numiddot lcr widc iEry d~ middot diliom Iugravech pnilm odoptcd nc b Iyo i_ m- doc =p cauld bclBmiddot

IIIlpliablc ibltty dio-ne fToble 1) F do dep cL po)mcn dli _ bull _ r- dobullbull _ aetiw bull and - 10 ltiIlt ~nfCIl ohe prcfcrrcd Us- the _ lb ooIwl Ugtd __tIy oIIc litlt libiliq tbc boltnf p_ AltanoIJ ir lgtftC necbullbull pcltshyfoc RCf_peooilk odIl1cUcircltXI 0( ltOnfIOOlnd lof tbc lDdlais cL bull phomuautioaIly le pouduco Ofpnilt oohc IJ diIf nl ~i ply bull the 1Hoo1you AI II Icirci rclthcc --- ocorilty tIZflH m- loIcgtpIlilif le OIIOIld bc _ ibIc clioicc r pptaiDn i__-dia 1~5I n Ji ~ ofn i_mc lOI ~ di ltIfICplile c dopltcd off yaciriof fIlin rOf 0 ~riuy 01 oppli(otiofto

_ hM TIocpliilt CJIrcmophilco ~ cd _ ne rion kul umophlllc ptOIlt_ li poIy-dqndil lt110) IUCh n dlul h bull d yI ~Vll rotlCl lb IAlI Inod il _10 IThIc 1~ Tho fCO-o 1)1

Otobk nd oeil ol ~cd PfM- obtmiddot A icnd nou he oolubilify 01 noy ion laquogtngtpOfInll in penk ltr po)merlc oubouo bull liancly cd MOfco~ dobullbullioIr 01 11oft ludinl 0 undctifld 00000pliulOf 1 uh ed hipMr

_ fin _ _ _ Il -1

bull

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Noe Too Hoc III H-IIo

R-- 1Cfed fiIo -bOre oi 1 IcircIlI shy

IcircII rirr _ ff71IlIlOdrncand how in Iorinamp -roba lld odoltr umpu io fwlcoOlUI r - lNbull odoolOd biolP bull~ ~

_pcnrr CUumlIHL 1111 il shydolt _ft o1dlouch IcircI _ _

~I diHu -l Ir dn For lltUlIIl bUl-1ooonIIl afnlll shy0I1fIle$ raaDbk lthoir beo-ocircoodonnl

dw _ O J _I-a 1r1Ugraveu middotoct io -- ~cl)lkal nIo il -IimI fi (- bull for HJV ~Rd~ - ru FOcirc OIOItIepacircbility 10 _____bull 1-shy shy

In lC1 - DNA poIltUJIOcirclIlur1onipptt01 DNA prodllQ 0__ p p)l Tho _011 roiaor 10 lIecyccd Ioorwcco lowarwl _W1IaoMshylion iol -l ~ohcpoty-- _ Ire- aobct tIlIf bullbulln ROC l Licnd 00 0f01lltd kmiddot

lbc Iwx plll 01 plcnd~bull Tlaquohnitwu hod rplmilh lhe en-IlMI -urIm le

To pcJ 11lQl oacnrion ecn plodcd 1 f shyampcm dculqhouoc wllen Ilrock kad dcpooiled pIa oupIy 10 fCIIlt lier The iIIlon Theft iooLotcd doo= m~ DNA poI lT poIr

Copytlplt llln $dfUIc ~l F

-1 1 ~ Ile for 11 IN lu 101 oIlUJIlly 0Uf0m2I

cd rat ~b-m rIOCDC of PCll ho roploced dw T~

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Adom-bc commcnioJ ~ fi linrtIcy w ~ allN C(~IR procMood fnIm tch CydoMlrxtrino ~ 10 lfabiliK talCultoIIo io frlnlthl In P doc ll4lab 01 mrdishyciDn br tIc body and hl -_ shy

odon onalitinet

cow an

_nr __Iho -lIr-shybodl - oI_mm drpaC(w JI F) a 0- Mlf lM oanhampco AoIlIIl1oftd __ cl dw Nnic lOlo Mshy1fUio pl wly dor for sshyp )r- plloshyn likc rhe tlorlfIf PPG Iik oh - oi MlthroplWoo (ltDld Iowcn~

Ju- T Sulqo ud Uuml -

~t=~ obioJ=-me lfUlI AntllClic bgtshyocean bullbullIf lhaI ffllllino froun for mudl cl yult TbrocCCOlll1WllIcirctico lashydudo uumldilcrilt karyabIJ alpe od dlofOllIS Il wdI Il bull ftlircy of borio 0 bolttnillbUMmiddot 1br Sulqo 1fOIIP oJ-p___dnmiddot io plinu ltpfe5ltnlgtti 01 PJIChro pIiIo oprisul prnnue lor -m io C od io fiumlogtdI -puashybom Il dtpeaCfOO kir uproclucocircoL CIl kbc OfF bull Iflllal fO im lUcircoawm who nccrl CDIJIbI dool rfriImiddot lIfQlltonar-- faocl __ 1-- produca uquifc w _~ ~

aukm oIlnampn_lhidl po

Il ~ ~11JtCl 01 Imiddotwbullbull tclry WPB A- exvemopQiln DO

uuml ll

bull

7 c en c Anlrri

1

) _ ln__

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no_ odo lIpoœit1lo Ill shy rill1oove ~-cl --Ss _ril il =0 i bull oIlltf1tlo wldl ft bull oM Il 4~_M -9ftft _ lot

Il 0-_ shy__-ftlIdNIl= r ------- = 7 1 11~ _111

_ ~ Fon) sJi t bullbull1 r U1 __0_ _ CIII_as__e- ~ bach _ _ lht I _ ~ d Itlt ~

--M__ ri 1 IS 1 d__01 100- =

t ~ bullbull 1Il~- shy lIcoi Noct ____No

lht s _ -__ _~_ --_---1

1CMn_ bull _

10 ___

0001( bull ~ 1J77 _ bull s _AJlIlN~ _~l _4 _dao1lo 1- _- -Mrt6shyIl -cuIwi tIUgraveMbIC-

Il _ m-Il~

na toi oH pblt fO fcK ~ nxIE III fonz Ullft IIgt dit llbIy foaMd ~ 71w fatiilalCl r ltIiopnIioIl encbl pupo ~ lItIon UcircIc oil WI pat

tc===4r$r IcircI tddaI jgoc bdo - jomI dolt shy1Ind _ Tf ca _ Ibo LfeOshy

liF - _ J ~ Aa bull 1 rio tJ __0 middotd ---a ~œbull Rr- -- ~ iwc S ncILIioiot __ -M

st _ca

w IId - tmiddot II _ = ~ loItroiNunli s Uoi ~

Il -u_)_~IcltoIc_

middotlJlllshy_ jIftIiNo woooW _--

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f T_~iuml+

bull fi n ldi-l- po- ltuhm 01 na rn u -If _ IR Iookont fol M ~

wli __

t Pt ho

11 7lltooblc

~DNA _~ 1 1 11_ doc 1 01 _ 1

loouumlo bull

-0 tz -d i1ioolI ~

-m t~iI~ 1 Icirc1idoou fi It_ bull dG) 100 = _alf

1CtdiIy aiochal_ Md ~ ~d r~~ ~ docl ---

bull i ~ J a 1ctW wlioltDfd Olt Il ad lLCiicy if ole 1 _hen 10 otl hnicoJ_ _ ~ mpoMlWo kir cho_ Doriry _g ha lmiddot_ _ doc LU lied rift iD oloocr M JUIcircOI doshy

bull ot in S

~bull--r CAO S 11lq dot DNA rr-~ _ -001 -wl n-n exshy_ao bullrn-_ __ DNA -1 u~

--li bull - shy -a_ _ li COlI i _

__ rilll _ shy IlIIHwI

Mt

S - -

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_ - 1IIri Insighu lnta _ 1Ulttgt 1Ifo- pl 10laquo_1 JqJiIo IboocWrioL d cd ~ œnoIn ail _ -anboonollclf ~IDfODC SdiwIIlIU OW ID n IN _ bIrbo8OislJdbdtcI~9In__bullbull 0I1O

u ID _ Gmn bIutpmu ifonI _ PIfbm -11lt1 induumllt __rltlIldampul ail he-N

- 1

i 1 1 1

1 hdp tIIZJGlel from onlimrp rnicngtba inm anilicial U1twfId

DiIrofflY cl~OC- 0jlfkW MImiddotmiddot br_de 1 i aI au_dM _ pobiluumla Y for lIeOI cuyme lU pin ~=pta-itomair ilI lia~ 10 kttp down Ihc (Otef 0 pt(gtshy

dacrion m~~~tIw -1 xm_r= prodllCilll tamporr will rdUbly mm al PfOI Tho dtfkpllilaquo of ~ ufKlUriaa trdIniquco and thc le-

Illqanceol iod Wltwe JJQ tl Uead e-bIr wd0llUId ~lItUJof -u (CiIlIa li _ ilwgtillb ~

or tbcilt _r aIP na nad proote irrnioriblo c

pm PfOIiUI Degtoota 01 he wshyet ~ known dirtard cYltgtlushy

OIlOl froaI

bon k Of 1 rudom nashyna the ~~al-

~ llW acnerthoo do of diffnm of the enzynw T1wn Ihey 0ClfII0l1hc shylU hnhee of lhe bullbullriItioGo pintd topod-b futurlt- Th Lui IUllteV il aloo Wd 10 he PdioonLon co ncn Ed -pt bull -loI 10 u bull 6ItmmT br doc liahlbtlb he lriod ~ ilable m bombou 10 oiIIr ttado nd dlott br_I WOIked bat

iCI far _ ntnlOltf1OtO in shyaM lime olœrod choir iampioal Mm n raQoltlaJ dollp NId Edooniaa ppruiOCha prumi la

halllaquo emlII(Iamp)lIl Tbey aloo

Loi

_____

-ER

Extremophiles as a source for novel enzymes Bertus van den BlXg

M_ lObe_ shy on bull _

bo_~ __ _ ft _--lI)r_oI_pH TI-__ _0 No IIogtOIIbool lnl

Cono po _al___ --10__ ~bull Ol_~ PlOC N __ 0l

J= bull _ EMII__ i __

1 __plt _ W_do bullbull_ - 01 _11__ _ - =ozrno _ __ lol 01 0 -pl _ _- r

_l _ Il4tIo_lIIllO_ _ Tho ----_---___ ____ la _11

1Ol __---shy~ 00 br l-lq lodulriIoI dc d fOI bioatayJ do lM COI _ cooodiltions id obIlt cfllwto ~ Nu -od co lh ~ rOI _h uyma cmoaI arpalc tloaio bIcircolIc31alyI emn lM boette cheinl pl oohilth con kod 10 _ cl5acircelo pnodian 01 10 gttltragtIumlODltIgtltB (cr bull tt iffNIrtlltlllll nkn Il ~ ~ilC Ibc ampa do ID do _ dun JOOO dlll_ euyma lM oori6ed or thcfCl dleir-~ iDdtauiol pIi_ he pracA UZ)1IM bon niU _ 1Iccediloe bull de A0a_ dl iam foct _y Iob euyma lN _ wilttltond ml ~

-Ji As rauk dM lt~ iu almia Ole ~ i~_C1I famp_ hot igt pcoc dcugrave oudo zac-phila gtIe _ cl ~1U 1~J~

Eure QOnd fCfc 10 pII)l coIre le _pc Je ndirlti_ bult ID _

_ u1gt Ii~ad pH (rhI Il loi vt doc couemovtoilco h bec idltn~d Olle bclonJ 0 1 01 Ile AIclI bull _bullbull bullbullny nunoophilc r dgtc ria ond uy roc i bullbull o Illy ItIcntlllcd od dlancuriud

TIoc _ion ilco C090ble rviY uncla no eootchfoollt __lion1 shy_nu h Icd _ doc _nies Mw _ becn Yod roc_ oondm- 1 d bull -idaob6c r do t do hbullbull hcgtIotood d IIlncucircofldy cboruotiocd r- lU pH bull HP _ lIcircIIn

1 ~ l aDd diocua 0CCCm pl bull shydlo d-IJ iIoNoliobullbullnd ~ 01 ~ r _pt-_shyAi illu cd ln Tobie 1 lM el licol COllall numiddot lcr widc iEry d~ middot diliom Iugravech pnilm odoptcd nc b Iyo i_ m- doc =p cauld bclBmiddot

IIIlpliablc ibltty dio-ne fToble 1) F do dep cL po)mcn dli _ bull _ r- dobullbull _ aetiw bull and - 10 ltiIlt ~nfCIl ohe prcfcrrcd Us- the _ lb ooIwl Ugtd __tIy oIIc litlt libiliq tbc boltnf p_ AltanoIJ ir lgtftC necbullbull pcltshyfoc RCf_peooilk odIl1cUcircltXI 0( ltOnfIOOlnd lof tbc lDdlais cL bull phomuautioaIly le pouduco Ofpnilt oohc IJ diIf nl ~i ply bull the 1Hoo1you AI II Icirci rclthcc --- ocorilty tIZflH m- loIcgtpIlilif le OIIOIld bc _ ibIc clioicc r pptaiDn i__-dia 1~5I n Ji ~ ofn i_mc lOI ~ di ltIfICplile c dopltcd off yaciriof fIlin rOf 0 ~riuy 01 oppli(otiofto

_ hM TIocpliilt CJIrcmophilco ~ cd _ ne rion kul umophlllc ptOIlt_ li poIy-dqndil lt110) IUCh n dlul h bull d yI ~Vll rotlCl lb IAlI Inod il _10 IThIc 1~ Tho fCO-o 1)1

Otobk nd oeil ol ~cd PfM- obtmiddot A icnd nou he oolubilify 01 noy ion laquogtngtpOfInll in penk ltr po)merlc oubouo bull liancly cd MOfco~ dobullbullioIr 01 11oft ludinl 0 undctifld 00000pliulOf 1 uh ed hipMr

_ fin _ _ _ Il -1

bull

7 c en c Anlrri

1

) _ ln__

tItIIdtIls Ihtllm riI INad__ itIArchatll Makn Thrlaquo ___edlnlUl

no_ odo lIpoœit1lo Ill shy rill1oove ~-cl --Ss _ril il =0 i bull oIlltf1tlo wldl ft bull oM Il 4~_M -9ftft _ lot

Il 0-_ shy__-ftlIdNIl= r ------- = 7 1 11~ _111

_ ~ Fon) sJi t bullbull1 r U1 __0_ _ CIII_as__e- ~ bach _ _ lht I _ ~ d Itlt ~

--M__ ri 1 IS 1 d__01 100- =

t ~ bullbull 1Il~- shy lIcoi Noct ____No

lht s _ -__ _~_ --_---1

1CMn_ bull _

10 ___

0001( bull ~ 1J77 _ bull s _AJlIlN~ _~l _4 _dao1lo 1- _- -Mrt6shyIl -cuIwi tIUgraveMbIC-

Il _ m-Il~

na toi oH pblt fO fcK ~ nxIE III fonz Ullft IIgt dit llbIy foaMd ~ 71w fatiilalCl r ltIiopnIioIl encbl pupo ~ lItIon UcircIc oil WI pat

tc===4r$r IcircI tddaI jgoc bdo - jomI dolt shy1Ind _ Tf ca _ Ibo LfeOshy

liF - _ J ~ Aa bull 1 rio tJ __0 middotd ---a ~œbull Rr- -- ~ iwc S ncILIioiot __ -M

st _ca

w IId - tmiddot II _ = ~ loItroiNunli s Uoi ~

Il -u_)_~IcltoIc_

middotlJlllshy_ jIftIiNo woooW _--

F- Jt-o 1 e--tIodl _ 01 DN- J IU) ~ a - am_ _ az AI _ J - shy ro- __bull __ 01 cu bull 5 -~ -b _ nr ~ bull DNA 4a ad_

f T_~iuml+

bull fi n ldi-l- po- ltuhm 01 na rn u -If _ IR Iookont fol M ~

wli __

t Pt ho

11 7lltooblc

~DNA _~ 1 1 11_ doc 1 01 _ 1

loouumlo bull

-0 tz -d i1ioolI ~

-m t~iI~ 1 Icirc1idoou fi It_ bull dG) 100 = _alf

1CtdiIy aiochal_ Md ~ ~d r~~ ~ docl ---

bull i ~ J a 1ctW wlioltDfd Olt Il ad lLCiicy if ole 1 _hen 10 otl hnicoJ_ _ ~ mpoMlWo kir cho_ Doriry _g ha lmiddot_ _ doc LU lied rift iD oloocr M JUIcircOI doshy

bull ot in S

~bull--r CAO S 11lq dot DNA rr-~ _ -001 -wl n-n exshy_ao bullrn-_ __ DNA -1 u~

--li bull - shy -a_ _ li COlI i _

__ rilll _ shy IlIIHwI

Mt

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_ - 1IIri Insighu lnta _ 1Ulttgt 1Ifo- pl 10laquo_1 JqJiIo IboocWrioL d cd ~ œnoIn ail _ -anboonollclf ~IDfODC SdiwIIlIU OW ID n IN _ bIrbo8OislJdbdtcI~9In__bullbull 0I1O

u ID _ Gmn bIutpmu ifonI _ PIfbm -11lt1 induumllt __rltlIldampul ail he-N

- 1

i 1 1 1

1 hdp tIIZJGlel from onlimrp rnicngtba inm anilicial U1twfId

DiIrofflY cl~OC- 0jlfkW MImiddotmiddot br_de 1 i aI au_dM _ pobiluumla Y for lIeOI cuyme lU pin ~=pta-itomair ilI lia~ 10 kttp down Ihc (Otef 0 pt(gtshy

dacrion m~~~tIw -1 xm_r= prodllCilll tamporr will rdUbly mm al PfOI Tho dtfkpllilaquo of ~ ufKlUriaa trdIniquco and thc le-

Illqanceol iod Wltwe JJQ tl Uead e-bIr wd0llUId ~lItUJof -u (CiIlIa li _ ilwgtillb ~

or tbcilt _r aIP na nad proote irrnioriblo c

pm PfOIiUI Degtoota 01 he wshyet ~ known dirtard cYltgtlushy

OIlOl froaI

bon k Of 1 rudom nashyna the ~~al-

~ llW acnerthoo do of diffnm of the enzynw T1wn Ihey 0ClfII0l1hc shylU hnhee of lhe bullbullriItioGo pintd topod-b futurlt- Th Lui IUllteV il aloo Wd 10 he PdioonLon co ncn Ed -pt bull -loI 10 u bull 6ItmmT br doc liahlbtlb he lriod ~ ilable m bombou 10 oiIIr ttado nd dlott br_I WOIked bat

iCI far _ ntnlOltf1OtO in shyaM lime olœrod choir iampioal Mm n raQoltlaJ dollp NId Edooniaa ppruiOCha prumi la

halllaquo emlII(Iamp)lIl Tbey aloo

Loi

_____

-ER

Extremophiles as a source for novel enzymes Bertus van den BlXg

M_ lObe_ shy on bull _

bo_~ __ _ ft _--lI)r_oI_pH TI-__ _0 No IIogtOIIbool lnl

Cono po _al___ --10__ ~bull Ol_~ PlOC N __ 0l

J= bull _ EMII__ i __

1 __plt _ W_do bullbull_ - 01 _11__ _ - =ozrno _ __ lol 01 0 -pl _ _- r

_l _ Il4tIo_lIIllO_ _ Tho ----_---___ ____ la _11

1Ol __---shy~ 00 br l-lq lodulriIoI dc d fOI bioatayJ do lM COI _ cooodiltions id obIlt cfllwto ~ Nu -od co lh ~ rOI _h uyma cmoaI arpalc tloaio bIcircolIc31alyI emn lM boette cheinl pl oohilth con kod 10 _ cl5acircelo pnodian 01 10 gttltragtIumlODltIgtltB (cr bull tt iffNIrtlltlllll nkn Il ~ ~ilC Ibc ampa do ID do _ dun JOOO dlll_ euyma lM oori6ed or thcfCl dleir-~ iDdtauiol pIi_ he pracA UZ)1IM bon niU _ 1Iccediloe bull de A0a_ dl iam foct _y Iob euyma lN _ wilttltond ml ~

-Ji As rauk dM lt~ iu almia Ole ~ i~_C1I famp_ hot igt pcoc dcugrave oudo zac-phila gtIe _ cl ~1U 1~J~

Eure QOnd fCfc 10 pII)l coIre le _pc Je ndirlti_ bult ID _

_ u1gt Ii~ad pH (rhI Il loi vt doc couemovtoilco h bec idltn~d Olle bclonJ 0 1 01 Ile AIclI bull _bullbull bullbullny nunoophilc r dgtc ria ond uy roc i bullbull o Illy ItIcntlllcd od dlancuriud

TIoc _ion ilco C090ble rviY uncla no eootchfoollt __lion1 shy_nu h Icd _ doc _nies Mw _ becn Yod roc_ oondm- 1 d bull -idaob6c r do t do hbullbull hcgtIotood d IIlncucircofldy cboruotiocd r- lU pH bull HP _ lIcircIIn

1 ~ l aDd diocua 0CCCm pl bull shydlo d-IJ iIoNoliobullbullnd ~ 01 ~ r _pt-_shyAi illu cd ln Tobie 1 lM el licol COllall numiddot lcr widc iEry d~ middot diliom Iugravech pnilm odoptcd nc b Iyo i_ m- doc =p cauld bclBmiddot

IIIlpliablc ibltty dio-ne fToble 1) F do dep cL po)mcn dli _ bull _ r- dobullbull _ aetiw bull and - 10 ltiIlt ~nfCIl ohe prcfcrrcd Us- the _ lb ooIwl Ugtd __tIy oIIc litlt libiliq tbc boltnf p_ AltanoIJ ir lgtftC necbullbull pcltshyfoc RCf_peooilk odIl1cUcircltXI 0( ltOnfIOOlnd lof tbc lDdlais cL bull phomuautioaIly le pouduco Ofpnilt oohc IJ diIf nl ~i ply bull the 1Hoo1you AI II Icirci rclthcc --- ocorilty tIZflH m- loIcgtpIlilif le OIIOIld bc _ ibIc clioicc r pptaiDn i__-dia 1~5I n Ji ~ ofn i_mc lOI ~ di ltIfICplile c dopltcd off yaciriof fIlin rOf 0 ~riuy 01 oppli(otiofto

_ hM TIocpliilt CJIrcmophilco ~ cd _ ne rion kul umophlllc ptOIlt_ li poIy-dqndil lt110) IUCh n dlul h bull d yI ~Vll rotlCl lb IAlI Inod il _10 IThIc 1~ Tho fCO-o 1)1

Otobk nd oeil ol ~cd PfM- obtmiddot A icnd nou he oolubilify 01 noy ion laquogtngtpOfInll in penk ltr po)merlc oubouo bull liancly cd MOfco~ dobullbullioIr 01 11oft ludinl 0 undctifld 00000pliulOf 1 uh ed hipMr

_ fin _ _ _ Il -1

bull

1

) _ ln__

tItIIdtIls Ihtllm riI INad__ itIArchatll Makn Thrlaquo ___edlnlUl

no_ odo lIpoœit1lo Ill shy rill1oove ~-cl --Ss _ril il =0 i bull oIlltf1tlo wldl ft bull oM Il 4~_M -9ftft _ lot

Il 0-_ shy__-ftlIdNIl= r ------- = 7 1 11~ _111

_ ~ Fon) sJi t bullbull1 r U1 __0_ _ CIII_as__e- ~ bach _ _ lht I _ ~ d Itlt ~

--M__ ri 1 IS 1 d__01 100- =

t ~ bullbull 1Il~- shy lIcoi Noct ____No

lht s _ -__ _~_ --_---1

1CMn_ bull _

10 ___

0001( bull ~ 1J77 _ bull s _AJlIlN~ _~l _4 _dao1lo 1- _- -Mrt6shyIl -cuIwi tIUgraveMbIC-

Il _ m-Il~

na toi oH pblt fO fcK ~ nxIE III fonz Ullft IIgt dit llbIy foaMd ~ 71w fatiilalCl r ltIiopnIioIl encbl pupo ~ lItIon UcircIc oil WI pat

tc===4r$r IcircI tddaI jgoc bdo - jomI dolt shy1Ind _ Tf ca _ Ibo LfeOshy

liF - _ J ~ Aa bull 1 rio tJ __0 middotd ---a ~œbull Rr- -- ~ iwc S ncILIioiot __ -M

st _ca

w IId - tmiddot II _ = ~ loItroiNunli s Uoi ~

Il -u_)_~IcltoIc_

middotlJlllshy_ jIftIiNo woooW _--

F- Jt-o 1 e--tIodl _ 01 DN- J IU) ~ a - am_ _ az AI _ J - shy ro- __bull __ 01 cu bull 5 -~ -b _ nr ~ bull DNA 4a ad_

f T_~iuml+

bull fi n ldi-l- po- ltuhm 01 na rn u -If _ IR Iookont fol M ~

wli __

t Pt ho

11 7lltooblc

~DNA _~ 1 1 11_ doc 1 01 _ 1

loouumlo bull

-0 tz -d i1ioolI ~

-m t~iI~ 1 Icirc1idoou fi It_ bull dG) 100 = _alf

1CtdiIy aiochal_ Md ~ ~d r~~ ~ docl ---

bull i ~ J a 1ctW wlioltDfd Olt Il ad lLCiicy if ole 1 _hen 10 otl hnicoJ_ _ ~ mpoMlWo kir cho_ Doriry _g ha lmiddot_ _ doc LU lied rift iD oloocr M JUIcircOI doshy

bull ot in S

~bull--r CAO S 11lq dot DNA rr-~ _ -001 -wl n-n exshy_ao bullrn-_ __ DNA -1 u~

--li bull - shy -a_ _ li COlI i _

__ rilll _ shy IlIIHwI

Mt

S - -

~1997k~IIt_ -

~ altcNCfIn_~lt-flIlhIt_b1IliI ~(Mlbl_lollhe _1) IIH ioMlt ~ II aphlic OIll9flMII$lIM 9 _ Mon fA lIMue nNNl9tfle$ ~

_ - 1IIri Insighu lnta _ 1Ulttgt 1Ifo- pl 10laquo_1 JqJiIo IboocWrioL d cd ~ œnoIn ail _ -anboonollclf ~IDfODC SdiwIIlIU OW ID n IN _ bIrbo8OislJdbdtcI~9In__bullbull 0I1O

u ID _ Gmn bIutpmu ifonI _ PIfbm -11lt1 induumllt __rltlIldampul ail he-N

- 1

i 1 1 1

1 hdp tIIZJGlel from onlimrp rnicngtba inm anilicial U1twfId

DiIrofflY cl~OC- 0jlfkW MImiddotmiddot br_de 1 i aI au_dM _ pobiluumla Y for lIeOI cuyme lU pin ~=pta-itomair ilI lia~ 10 kttp down Ihc (Otef 0 pt(gtshy

dacrion m~~~tIw -1 xm_r= prodllCilll tamporr will rdUbly mm al PfOI Tho dtfkpllilaquo of ~ ufKlUriaa trdIniquco and thc le-

Illqanceol iod Wltwe JJQ tl Uead e-bIr wd0llUId ~lItUJof -u (CiIlIa li _ ilwgtillb ~

or tbcilt _r aIP na nad proote irrnioriblo c

pm PfOIiUI Degtoota 01 he wshyet ~ known dirtard cYltgtlushy

OIlOl froaI

bon k Of 1 rudom nashyna the ~~al-

~ llW acnerthoo do of diffnm of the enzynw T1wn Ihey 0ClfII0l1hc shylU hnhee of lhe bullbullriItioGo pintd topod-b futurlt- Th Lui IUllteV il aloo Wd 10 he PdioonLon co ncn Ed -pt bull -loI 10 u bull 6ItmmT br doc liahlbtlb he lriod ~ ilable m bombou 10 oiIIr ttado nd dlott br_I WOIked bat

iCI far _ ntnlOltf1OtO in shyaM lime olœrod choir iampioal Mm n raQoltlaJ dollp NId Edooniaa ppruiOCha prumi la

halllaquo emlII(Iamp)lIl Tbey aloo

Loi

_____

-ER

Extremophiles as a source for novel enzymes Bertus van den BlXg

M_ lObe_ shy on bull _

bo_~ __ _ ft _--lI)r_oI_pH TI-__ _0 No IIogtOIIbool lnl

Cono po _al___ --10__ ~bull Ol_~ PlOC N __ 0l

J= bull _ EMII__ i __

1 __plt _ W_do bullbull_ - 01 _11__ _ - =ozrno _ __ lol 01 0 -pl _ _- r

_l _ Il4tIo_lIIllO_ _ Tho ----_---___ ____ la _11

1Ol __---shy~ 00 br l-lq lodulriIoI dc d fOI bioatayJ do lM COI _ cooodiltions id obIlt cfllwto ~ Nu -od co lh ~ rOI _h uyma cmoaI arpalc tloaio bIcircolIc31alyI emn lM boette cheinl pl oohilth con kod 10 _ cl5acircelo pnodian 01 10 gttltragtIumlODltIgtltB (cr bull tt iffNIrtlltlllll nkn Il ~ ~ilC Ibc ampa do ID do _ dun JOOO dlll_ euyma lM oori6ed or thcfCl dleir-~ iDdtauiol pIi_ he pracA UZ)1IM bon niU _ 1Iccediloe bull de A0a_ dl iam foct _y Iob euyma lN _ wilttltond ml ~

-Ji As rauk dM lt~ iu almia Ole ~ i~_C1I famp_ hot igt pcoc dcugrave oudo zac-phila gtIe _ cl ~1U 1~J~

Eure QOnd fCfc 10 pII)l coIre le _pc Je ndirlti_ bult ID _

_ u1gt Ii~ad pH (rhI Il loi vt doc couemovtoilco h bec idltn~d Olle bclonJ 0 1 01 Ile AIclI bull _bullbull bullbullny nunoophilc r dgtc ria ond uy roc i bullbull o Illy ItIcntlllcd od dlancuriud

TIoc _ion ilco C090ble rviY uncla no eootchfoollt __lion1 shy_nu h Icd _ doc _nies Mw _ becn Yod roc_ oondm- 1 d bull -idaob6c r do t do hbullbull hcgtIotood d IIlncucircofldy cboruotiocd r- lU pH bull HP _ lIcircIIn

1 ~ l aDd diocua 0CCCm pl bull shydlo d-IJ iIoNoliobullbullnd ~ 01 ~ r _pt-_shyAi illu cd ln Tobie 1 lM el licol COllall numiddot lcr widc iEry d~ middot diliom Iugravech pnilm odoptcd nc b Iyo i_ m- doc =p cauld bclBmiddot

IIIlpliablc ibltty dio-ne fToble 1) F do dep cL po)mcn dli _ bull _ r- dobullbull _ aetiw bull and - 10 ltiIlt ~nfCIl ohe prcfcrrcd Us- the _ lb ooIwl Ugtd __tIy oIIc litlt libiliq tbc boltnf p_ AltanoIJ ir lgtftC necbullbull pcltshyfoc RCf_peooilk odIl1cUcircltXI 0( ltOnfIOOlnd lof tbc lDdlais cL bull phomuautioaIly le pouduco Ofpnilt oohc IJ diIf nl ~i ply bull the 1Hoo1you AI II Icirci rclthcc --- ocorilty tIZflH m- loIcgtpIlilif le OIIOIld bc _ ibIc clioicc r pptaiDn i__-dia 1~5I n Ji ~ ofn i_mc lOI ~ di ltIfICplile c dopltcd off yaciriof fIlin rOf 0 ~riuy 01 oppli(otiofto

_ hM TIocpliilt CJIrcmophilco ~ cd _ ne rion kul umophlllc ptOIlt_ li poIy-dqndil lt110) IUCh n dlul h bull d yI ~Vll rotlCl lb IAlI Inod il _10 IThIc 1~ Tho fCO-o 1)1

Otobk nd oeil ol ~cd PfM- obtmiddot A icnd nou he oolubilify 01 noy ion laquogtngtpOfInll in penk ltr po)merlc oubouo bull liancly cd MOfco~ dobullbullioIr 01 11oft ludinl 0 undctifld 00000pliulOf 1 uh ed hipMr

_ fin _ _ _ Il -1

bull

~ altcNCfIn_~lt-flIlhIt_b1IliI ~(Mlbl_lollhe _1) IIH ioMlt ~ II aphlic OIll9flMII$lIM 9 _ Mon fA lIMue nNNl9tfle$ ~

_ - 1IIri Insighu lnta _ 1Ulttgt 1Ifo- pl 10laquo_1 JqJiIo IboocWrioL d cd ~ œnoIn ail _ -anboonollclf ~IDfODC SdiwIIlIU OW ID n IN _ bIrbo8OislJdbdtcI~9In__bullbull 0I1O

u ID _ Gmn bIutpmu ifonI _ PIfbm -11lt1 induumllt __rltlIldampul ail he-N

- 1

i 1 1 1

1 hdp tIIZJGlel from onlimrp rnicngtba inm anilicial U1twfId

DiIrofflY cl~OC- 0jlfkW MImiddotmiddot br_de 1 i aI au_dM _ pobiluumla Y for lIeOI cuyme lU pin ~=pta-itomair ilI lia~ 10 kttp down Ihc (Otef 0 pt(gtshy

dacrion m~~~tIw -1 xm_r= prodllCilll tamporr will rdUbly mm al PfOI Tho dtfkpllilaquo of ~ ufKlUriaa trdIniquco and thc le-

Illqanceol iod Wltwe JJQ tl Uead e-bIr wd0llUId ~lItUJof -u (CiIlIa li _ ilwgtillb ~

or tbcilt _r aIP na nad proote irrnioriblo c

pm PfOIiUI Degtoota 01 he wshyet ~ known dirtard cYltgtlushy

OIlOl froaI

bon k Of 1 rudom nashyna the ~~al-

~ llW acnerthoo do of diffnm of the enzynw T1wn Ihey 0ClfII0l1hc shylU hnhee of lhe bullbullriItioGo pintd topod-b futurlt- Th Lui IUllteV il aloo Wd 10 he PdioonLon co ncn Ed -pt bull -loI 10 u bull 6ItmmT br doc liahlbtlb he lriod ~ ilable m bombou 10 oiIIr ttado nd dlott br_I WOIked bat

iCI far _ ntnlOltf1OtO in shyaM lime olœrod choir iampioal Mm n raQoltlaJ dollp NId Edooniaa ppruiOCha prumi la

halllaquo emlII(Iamp)lIl Tbey aloo

Loi

_____

-ER

Extremophiles as a source for novel enzymes Bertus van den BlXg

M_ lObe_ shy on bull _

bo_~ __ _ ft _--lI)r_oI_pH TI-__ _0 No IIogtOIIbool lnl

Cono po _al___ --10__ ~bull Ol_~ PlOC N __ 0l

J= bull _ EMII__ i __

1 __plt _ W_do bullbull_ - 01 _11__ _ - =ozrno _ __ lol 01 0 -pl _ _- r

_l _ Il4tIo_lIIllO_ _ Tho ----_---___ ____ la _11

1Ol __---shy~ 00 br l-lq lodulriIoI dc d fOI bioatayJ do lM COI _ cooodiltions id obIlt cfllwto ~ Nu -od co lh ~ rOI _h uyma cmoaI arpalc tloaio bIcircolIc31alyI emn lM boette cheinl pl oohilth con kod 10 _ cl5acircelo pnodian 01 10 gttltragtIumlODltIgtltB (cr bull tt iffNIrtlltlllll nkn Il ~ ~ilC Ibc ampa do ID do _ dun JOOO dlll_ euyma lM oori6ed or thcfCl dleir-~ iDdtauiol pIi_ he pracA UZ)1IM bon niU _ 1Iccediloe bull de A0a_ dl iam foct _y Iob euyma lN _ wilttltond ml ~

-Ji As rauk dM lt~ iu almia Ole ~ i~_C1I famp_ hot igt pcoc dcugrave oudo zac-phila gtIe _ cl ~1U 1~J~

Eure QOnd fCfc 10 pII)l coIre le _pc Je ndirlti_ bult ID _

_ u1gt Ii~ad pH (rhI Il loi vt doc couemovtoilco h bec idltn~d Olle bclonJ 0 1 01 Ile AIclI bull _bullbull bullbullny nunoophilc r dgtc ria ond uy roc i bullbull o Illy ItIcntlllcd od dlancuriud

TIoc _ion ilco C090ble rviY uncla no eootchfoollt __lion1 shy_nu h Icd _ doc _nies Mw _ becn Yod roc_ oondm- 1 d bull -idaob6c r do t do hbullbull hcgtIotood d IIlncucircofldy cboruotiocd r- lU pH bull HP _ lIcircIIn

1 ~ l aDd diocua 0CCCm pl bull shydlo d-IJ iIoNoliobullbullnd ~ 01 ~ r _pt-_shyAi illu cd ln Tobie 1 lM el licol COllall numiddot lcr widc iEry d~ middot diliom Iugravech pnilm odoptcd nc b Iyo i_ m- doc =p cauld bclBmiddot

IIIlpliablc ibltty dio-ne fToble 1) F do dep cL po)mcn dli _ bull _ r- dobullbull _ aetiw bull and - 10 ltiIlt ~nfCIl ohe prcfcrrcd Us- the _ lb ooIwl Ugtd __tIy oIIc litlt libiliq tbc boltnf p_ AltanoIJ ir lgtftC necbullbull pcltshyfoc RCf_peooilk odIl1cUcircltXI 0( ltOnfIOOlnd lof tbc lDdlais cL bull phomuautioaIly le pouduco Ofpnilt oohc IJ diIf nl ~i ply bull the 1Hoo1you AI II Icirci rclthcc --- ocorilty tIZflH m- loIcgtpIlilif le OIIOIld bc _ ibIc clioicc r pptaiDn i__-dia 1~5I n Ji ~ ofn i_mc lOI ~ di ltIfICplile c dopltcd off yaciriof fIlin rOf 0 ~riuy 01 oppli(otiofto

_ hM TIocpliilt CJIrcmophilco ~ cd _ ne rion kul umophlllc ptOIlt_ li poIy-dqndil lt110) IUCh n dlul h bull d yI ~Vll rotlCl lb IAlI Inod il _10 IThIc 1~ Tho fCO-o 1)1

Otobk nd oeil ol ~cd PfM- obtmiddot A icnd nou he oolubilify 01 noy ion laquogtngtpOfInll in penk ltr po)merlc oubouo bull liancly cd MOfco~ dobullbullioIr 01 11oft ludinl 0 undctifld 00000pliulOf 1 uh ed hipMr

_ fin _ _ _ Il -1

bull

_____

-ER

Extremophiles as a source for novel enzymes Bertus van den BlXg

M_ lObe_ shy on bull _

bo_~ __ _ ft _--lI)r_oI_pH TI-__ _0 No IIogtOIIbool lnl

Cono po _al___ --10__ ~bull Ol_~ PlOC N __ 0l

J= bull _ EMII__ i __

1 __plt _ W_do bullbull_ - 01 _11__ _ - =ozrno _ __ lol 01 0 -pl _ _- r

_l _ Il4tIo_lIIllO_ _ Tho ----_---___ ____ la _11

1Ol __---shy~ 00 br l-lq lodulriIoI dc d fOI bioatayJ do lM COI _ cooodiltions id obIlt cfllwto ~ Nu -od co lh ~ rOI _h uyma cmoaI arpalc tloaio bIcircolIc31alyI emn lM boette cheinl pl oohilth con kod 10 _ cl5acircelo pnodian 01 10 gttltragtIumlODltIgtltB (cr bull tt iffNIrtlltlllll nkn Il ~ ~ilC Ibc ampa do ID do _ dun JOOO dlll_ euyma lM oori6ed or thcfCl dleir-~ iDdtauiol pIi_ he pracA UZ)1IM bon niU _ 1Iccediloe bull de A0a_ dl iam foct _y Iob euyma lN _ wilttltond ml ~

-Ji As rauk dM lt~ iu almia Ole ~ i~_C1I famp_ hot igt pcoc dcugrave oudo zac-phila gtIe _ cl ~1U 1~J~

Eure QOnd fCfc 10 pII)l coIre le _pc Je ndirlti_ bult ID _

_ u1gt Ii~ad pH (rhI Il loi vt doc couemovtoilco h bec idltn~d Olle bclonJ 0 1 01 Ile AIclI bull _bullbull bullbullny nunoophilc r dgtc ria ond uy roc i bullbull o Illy ItIcntlllcd od dlancuriud

TIoc _ion ilco C090ble rviY uncla no eootchfoollt __lion1 shy_nu h Icd _ doc _nies Mw _ becn Yod roc_ oondm- 1 d bull -idaob6c r do t do hbullbull hcgtIotood d IIlncucircofldy cboruotiocd r- lU pH bull HP _ lIcircIIn

1 ~ l aDd diocua 0CCCm pl bull shydlo d-IJ iIoNoliobullbullnd ~ 01 ~ r _pt-_shyAi illu cd ln Tobie 1 lM el licol COllall numiddot lcr widc iEry d~ middot diliom Iugravech pnilm odoptcd nc b Iyo i_ m- doc =p cauld bclBmiddot

IIIlpliablc ibltty dio-ne fToble 1) F do dep cL po)mcn dli _ bull _ r- dobullbull _ aetiw bull and - 10 ltiIlt ~nfCIl ohe prcfcrrcd Us- the _ lb ooIwl Ugtd __tIy oIIc litlt libiliq tbc boltnf p_ AltanoIJ ir lgtftC necbullbull pcltshyfoc RCf_peooilk odIl1cUcircltXI 0( ltOnfIOOlnd lof tbc lDdlais cL bull phomuautioaIly le pouduco Ofpnilt oohc IJ diIf nl ~i ply bull the 1Hoo1you AI II Icirci rclthcc --- ocorilty tIZflH m- loIcgtpIlilif le OIIOIld bc _ ibIc clioicc r pptaiDn i__-dia 1~5I n Ji ~ ofn i_mc lOI ~ di ltIfICplile c dopltcd off yaciriof fIlin rOf 0 ~riuy 01 oppli(otiofto

_ hM TIocpliilt CJIrcmophilco ~ cd _ ne rion kul umophlllc ptOIlt_ li poIy-dqndil lt110) IUCh n dlul h bull d yI ~Vll rotlCl lb IAlI Inod il _10 IThIc 1~ Tho fCO-o 1)1

Otobk nd oeil ol ~cd PfM- obtmiddot A icnd nou he oolubilify 01 noy ion laquogtngtpOfInll in penk ltr po)merlc oubouo bull liancly cd MOfco~ dobullbullioIr 01 11oft ludinl 0 undctifld 00000pliulOf 1 uh ed hipMr

_ fin _ _ _ Il -1

bull

----

t ~tbullbull

shy- --

_Idi llI~_~-- shy-__ shy-~~~~-~~--- shyn 1 - 10 _ bull _Ill bullbullbull---shy--~_c P_ ~ __

i nl~CC _

bull bullC P__ i- shybull 10 _o p _

_ 1 bull- bullCP

_- r 1

~ shy

p=j

--shy1 _ bullbull

CIOshy~-0 __0-_bull 1shyn 00nI- 0

_~c1 il

_~

- bull P -~ ~

bull bull bull 1-

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