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Sample Collection: • Fish were collected in the summer fall and winter of 2011 and the spring of 2012 from 8 sites along the Athabasca Slave and Peace Rivers. • Target sample size for each site was 30 individuals of each of 5 species (Goldeye - Hiodon alosoides, Whitefish - Coregonus clupeaformis, Northern Pike - Esox lucius, Walleye - Sander vitreus, Burbot - Lota lota). • A total of 1498 fish were collected during these samplings. • Each fish was examined externally and internally. • Muscle, liver, bile, and blood plasma samples were taken for further analysis of indicators of condition and health.
Introduc4on Metal Site BB Mean ± SE (n) * p-‐value GE Mean ± SE (n)* p-‐value JF Mean ± SE (n)* p-‐value WE Mean ± SE (n)* p-‐value WF Mean ±SE (n)* p-‐value Hg FMU 540±81 (3) 0.712 1251±252 (11) A 0.678 1416±256 (10) A 0.240 2475±733 (12) A <0.001 514±134 (10) A <0.001
FM 691±162 (2) 1022±95 (20) A 1397±371 (19) A 1309±139 (20) A 179±21 (20) C FC 340±24 (4) 952±102 (19) A 1294±158 (20) A 811±78 (15) B 190±38 (20) C FS 734±114 (8) A 973±125 (20) A 1427±311 (21) X 0.010 1813±264 (20) A 216±32 (18) B FR 839±116 (30) A 1241±188 (12) A 1031±190 (21) A 1399±306 (10) A 353±44 (20) A
Se FMU 1848±166 (3) 0.308 3060±340 (11) AB 0.005 1205±106 (13) B <0.001 1717±146 (13) BC <0.001 1612±133 (10) BC <0.001 FM 1476±121 (2) 2397±205 (20) B 843±118 (19) C 1432±137 (20) C 1546±78 (19) C FC 2282±164 (4) 3262±216 (19) AB 1639±105 (20) A 2041±106 (15) AB 1509±105 (20) C FS 1984±201 (8) A 3549±353 (20) A 1879±80 (21) A 2338±108 (20) A 2069±136 (18) AB FR 1806±71 (30) A 4053±456 (12) A 1869±94 (21) A 2303±94 (10) AB 2233±89 (20) A
Tl FMU 8.94±0.42 (3) 0.003 11.51±1.50 (11) A 0.234 14.45±2.36 (13) B <0.001 20.94±3.61 (13) C <0.001 6.75±1.51 (10) B <0.001 FM 0.05 (2) 9.26±1.28 (20) A 8.02±1.32 (19) B 19.96±3.10 (20) C 6.32±0.70 (20) B FC 8.88±3.38 (4) 12.04±2.13 (19) A 16.84±2.22 (20) B 37.4±2.00 (15) B 6.85±1.26 (20) B FS 8.43±1.36 (8) B 13.70±1.82 (20) A 32.82±4.00 (21) A 66.16±6.97 (20) A 20.30±2.70 (18) A FR 15.99±1.53 (30) A 15.44±2.98 (12) A 47.72±5.41 (21) A 78.54±7.96 (10) A 17.59±1.19 (20) A
V FMU 46.6±2.0 (3) 0.031 22.3±5.4 (11) A 0.141 22.3±4.4 (13) A 0.208 25.3±5.8 (13) A 0.265 35.7±8.0 (10) A 0.096 FM 20.5±2.1 (2) 20.9±3.4 (20) A 16.3±2.8 (19) A 16.8±3.6 (20) A 18.8±3.4 (20) A FC 12.7±3.2 (4) 18.5±3.8 (19) A 23.7±5.1 (20) A 25.5±8.0 (15) A 37.0±5.6 (20) A FS 66.3±16.4 (8) A 35.6±8.7 (20) A 25.0±5.7 (21) A 34.1±6.4 (20) A 40.7±8.4 (18) A FR 28.8±3.6 (30) B 36.0±4.2 (12) A 14.0±4.0 (21) A 17.7±5.7 (10) A 49.2±16.5 (20) A
Objec4ves
• Much of the current open-pit mining activities in the Alberta oilsand are adjacent to the Athabasca River. • Contaminants associated with oilsands include metals, polycyclic aromatic hydrocarbons (PAHs), and naphthenic acids, some of which are persistent, toxic, mutagenic and potentially carcinogenic. • Concerns have been expressed about the potential effects of emissions on the environment and human health by communities in the Athabasca River basin as well as by communities in the downstream Slave and Mackenzie river basins. • Local communities have also reported an increase in the occurrence of lesions and deformities in fishes which has raised their concerns • These concerns have been heightened by uncertainties concerning the origin of contaminants, natural or anthropogenic, and magnitudes of exposure.
Methods
Conclusions • Concentrations of Se were different in summer and fall. The increased
concentration in summer could be caused by the influx of contaminants after snow melt.
• Thallium, but not V or Se appear to biomagnify, since fishes of higher trophic levels have equal or higher concentrations than those at lower trophic levels.
• V and Tl have been previously associated with discharges from petroleum based activities, including oil sands activities.
• Greater concentrations of metals along the Slave River are potentially due to upstream activities.
• Increases in V and Tl at the downstream sites pose interesting questions of bioaccumulation and chemodynamics, further research is planned.
• Spring sampling has been completed and metals are being quantified to better understand spatial and temporal distribution of their concentrations.
• Two sites were added downstream of Athabasca Basin for the spring sampling (FF, PP) to better understand metal distribution.
Acknowledgements This project would not have been possible without the assistance provided by First Nations and Métis communities of Ft Resolution, Ft Smith, Ft Chipewyan, Ft MacKay and Ft McMurray and by numerous Provincial and Federal agencies. Funding was provided by the Boreal Songbird Initiative (BSI), Aboriginal Affairs and Northern Development Canada (AANDC) and the Government of the Northwest Territories, which supported sampling in the NWT. Tim Barrett (UNB) provided assistance with statistical analysis.
This research was conducted to describe spatial and temporal distributions of metals in tissues of fishes from selected locations along the Athabasca and Slave Rivers.
• Most of the metals analysed showed little or no location associated variability for the summer and fall samplings. • Thallium(Tl), Vanadium(V), Selenium(Se), and Mercury(Hg) did show location associated variability. • Se concentrations varied little among sites in summer, but in the fall were greater on the Slave River. • A steady increase in the concentration of thallium in fish muscle was noted at sites proceeding down the Athabasca/Slave system. • Thallium also showed a relationship between concentration and trophic level as the higher trophic level JF and WE had greater concentrations.
Results
Summer
B. Tendler1, E. Ohiozebau,2 A. Hill1, G. Codling2, J.P. Giesy1,3,4, E. Kelly5, P. Hodson6, J. Short7, P.D. Jones1,2 1.Toxicology Centre, University of Saskatchewan, Saskatoon, Canada 2.School of Environment & Sustainability, University of Saskatchewan, Saskatoon, Canada 3. Dept. of Biomedical Veterinary Sciences, University of Saskatchewan, Saskatoon, Canada
4. Dept. of Biology and Chemistry, City University of Hong Kong, Hong Kong, SAR, China 5. Environment and Natural Resources, Government of the Northwest Territories, Canada 6. Department of Biology, Queens University, Kingston ON, Canada 7. DJWS Consul.ng LLC, Juneau AK, USA
Exposure of Fish in the Athabasca and Slave Rivers to Metals Poten4ally Derived from Oilsands Opera4ons
Quantification of Metals: • Approximately 0.1 g of dry muscle was added to a 15 mL Teflon vial and digested in concentrated nitric acid (69%) and hydrogen peroxide (30%). • Digests were evaporated until dry at approximately 70ᴼC. • 5 mL of 2% nitric acid was added to dried digest which was then filtered (0.45 micrometre pore size) and transferred into 8 mL HDPE vial for storage and analysis. • Concentrations of 20 metals were measured by ICP-MS.
Methods
Pike or Jackfish (Esox lucius) JF
Loche Mariah, Burbot (Lota lota) BB
Goldeye (Hiodon alosoides) GE
Whitefish (Coregonus clupeaformis) WF
Walleye or Pickeral (Sander vitreus) WE
Figure 3. Concentrations of metals by species and site. Summary statistics (means, SE = standard error of the mean; n = sample size). Sites sharing a letter (A,B,C), within a metal/species grouping are not significantly different at α = 0.05 (Tukey family-wise error rate for multiple comparisons). Hypothesis tests conducted using ANOVA after data transformation (rank, log base 10, or power). Hg analyses conducted using ANCOVA with log base 10 transformed data (response and covariate) using length or weight as a covariate). X = significant interaction in ANCOVA (p = 0.05). Text in red addresses to subjectively assess trends across species.
Fall
BB GE JF WE WF decrease downriver
Hg increase at FS
Se increase at FR
Tl increase downriver
V increase at FS, not high upstream Athabasca
Results
Figure 1. Metal Concentration trends for metals of interest. Figure 2. Metal concentrations (V, Tl, Se) for all Species by Season.
• Concentrations of Vanadium (V) were significantly (p<0.05) greater at Fort Resolution and Fort Smith on the Slave River than those in fish from sites on the Athabasca River. • Concentrations of Selenium (Se) varied little among locations during summer. During the fall, selenium concentrations were greater in Slave River than Athabasca River. • Concentrations of Mercury (Hg) were greater in JF and WE on the Slave River.
Results