Exon 1
1
Exon 1 Exon 2 Exon 1 - Exon 2 Exon 1- Exon 2 Hybridization: - mix the two purified exon DNAs - program : 5 min at 95°C; - then 15 X 45s at 95°C, 1 min at 55°C, 1 min 30 sec at 72°C. Amplification by PCR: - addition of the flanking oligos (red) - canonical PCR program. Exon 1- Exon 2
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Exon 1. Exon 2. Hybridization: - mix the two purified exon DNAs program : 5 min at 95°C; then 15 X 45s at 95°C, 1 min at 55°C, 1 min 30 sec at 72°C. Exon 1- Exon 2. Exon 1- Exon 2. Amplification by PCR: - addition of the flanking oligos (red) - canonical PCR program. - PowerPoint PPT Presentation
Transcript of Exon 1
Exon 1 Exon 2
Exon 1 - Exon 2
Exon 1- Exon 2
Hybridization:- mix the two purified exon DNAs- program : 5 min at 95°C; - then 15 X 45s at 95°C, 1 min at 55°C, 1 min 30 sec at 72°C.
Amplification by PCR:- addition of the flanking oligos (red)- canonical PCR program.
Exon 1- Exon 2
![CRISPR/Cas9-mediated genome editing induces exon skipping ... · HeLa cells can cause skipping of exon 3, exon 4, or exons 3, 4, and 5 [18]. We also detected infrequent exon skipping](https://static.fdocuments.net/doc/165x107/60db8f117fb86d112c69c947/crisprcas9-mediated-genome-editing-induces-exon-skipping-hela-cells-can-cause.jpg)
