Ex. 15 Oxidase Test Objectives: ?? Cytochrome oxidases help with transfer of electrons from...
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Transcript of Ex. 15 Oxidase Test Objectives: ?? Cytochrome oxidases help with transfer of electrons from...
Ex. 15 Oxidase Test
Objectives: ??
Cytochrome oxidases help with transfer of electrons from cytochromes to oxygen.
Electron Transport Phosphorylation
Textbook Fig 5.16
Oxidase Test
• Determines presence of certain cytochrome oxidases
• Use whenever Gram-neg. bacteria have to be identified
• When test reagent gets oxidized it changes color.
• Oxidase test is positive if color change within 15 seconds!
Only use on young cultures
Table 15-1: Oxidase Test Results and Interpretation
Result Interpretation Symbol
Dark blue within 15 seconds Cytochrome c oxidase is present +
No color change to blue or blue after 15 seconds
Cytochrome c oxidase is absent
Oxistrip
The End
Materials needed per table: Oxidase reagent (bottle with
dropper) Small pieces of paper towel sterile Q-tips (2 per student)
Pure slant culture needed per table:1. E. coli2. Pseudomonas aeruginosa
Each student should individually do the oxidase test for both bacterial species.