Evolution at the Nucleotide Level
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Transcript of Evolution at the Nucleotide Level
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Evolution at the Nucleotide Level
Rapid Evolution of HIV/SIV and Microsatellites in Candida albicans
Matthew D. Pagel
NSCORT/Exobiology Fellow
Christopher Wills Lab
Department of Biology, University of California - San Diego
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HIV/SIV Phylogenetic Trees
A Model for Rapid Evolution
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Sample Sequence Alignment
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HIV/SIV Env gene Neighbor-
Joining Tree(from Yellow Fever, Black Goddess
by C. Wills)
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Neighbor-Joining Tree of Env gene of HIV/SIV - NCBI/Los Alamos Complete Sequences
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Pol Neighbor-Joining Tree
HIV1
HIV2
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Neighbor-Joining
Tree of Gag Gene of HIV/SIV
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NJ Tree of Gag after Unverified Sequences
Removed
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Cloning of Candida albicans genes involved in pathogenicity
Plasmid Construction of Erk1
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Microsatellite Slippage Mutations (Glutamine)
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The Genetic Code--The Translation of mRNA to Amino Acids--
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Recombination-Mediated Plasmid Construction
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Action of Restriction Enzymes(Eco RI example clipped from Molecular Cell Biology CD-Rom)
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Plasmid Constructionvia Restriction Digest
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Blue/White Screen
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Future Steps
• Site-directed mutagenesis producing variant microsatellite length
• Re-insertion of plasmid into knockout strains
• Analysis of morphological form (hyphal)
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Acknowledgements
Chris Wills
Nikki Freed
Dave Metzgar
Sol Guiterrez
Hattie Oswald
Lois Lane
Bill Baity
Carlos Flores, Vincent Starai, Carlos Peralta, & William Engels
The NSCORT/Exobiology Program