Evolution 300Evolution 600

UV-Vis Spectrophotometers

Life Science

Material Science

Pharmaceutical

m o l e c u l a r s p e c t r o s c o p y

Part of Thermo Fisher Scientific

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Reliable, High-Performance UV-Visible Spectrophotometers

For over 60 years, customers in world-renowned research institutions, highly regulated QC laboratories and teaching institutions have relied on Thermo Scientific UV-Visible (UV-Vis) spectrophotometers. The Evolution™ UV-Visible 300/600 spectrophotometer series is founded on the successful legacy of the Pye-Unicam, Bausch & Lomb, Philips Scientific,and Spectronic products. Many customers today say they made their first absorbance measurement on a SPECTRONIC™ 20 spectrophotometer, a product on the market since1953. The Evolution 300/600 series continues in this tradition by delivering the solid, high-performance you need for your UV-Vis applications.

The PU8800, designed by Pye Unicam inthe 1980’s, was one of the industry-leadingresearch instruments of its time.

Contents

Optical Design 4

System Configuration 6

Life Science Biochemical 8

Pharmaceutical 10& QC Analysis

Material Science 12

Smart Accessories 14

Thermo Scientific UV-VisSpectrophotometer Product LinesSPECTRONIC 20/20D, SPECTRONIC Educator™,and GENESYS™ 20

• Basic teaching• Easy sample and data handling

GENESYS 10 Series• Visible, UV-Vis, and UV-Vis scanning options• Routine quantitative analysis and common bio assays• Easy-to-use accessories

Helios™, Evolution 60, and Evolution 100• Routine quantitative analysis and bio assays• More advanced accessories and software

Evolution 300/600 Series• Wide range of accessories• Advanced quantitative analysis and bio assays• Extensive software offerings• Tablet dissolution• Material science

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Life Science

Material SciencePharmaceutical

QC

BiochemicalThe Evolution 300/600 spectrophotometer seriesincorporates a high-performance optical design, versatileVISION™ software packages, and the highest qualityaccessories for the most demanding applications. Thesystems offer a multitude of advantages for a wide variety of applications including:

• Life Science Biochemical• Pharmaceutical & QC• Material Science

Innovative Smart Accessories™ offer ease of use andincreased productivity by providing:

• Snap-and-go installation• Seamless software integration• Serial number tracking for the regulated environment

System configuration choices offer the ultimate in flexibility for your laboratory. Choose from:

• Stand-alone Local Control version• PC Control version• Combination of both

Evolution 300 & Evolution 600

UV-Vis Spectrophotom

eters

High-Performance Optical Design for Reliable Results

Maintaining high performance is the ongoing legacy you can count onwith the Thermo Scientific Evolution 300/600 spectrophotometer series.

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Mercury Lamp Primary referencestandard, allows

seamless validationand calibration of

the instrument

Light SourceXenon flash lamp

is only on when taking measurementsextending its lifetime

Optics Quartz overcoatedoptics for long-lasting reflectivity and cleaning withoutdamaging the optical surface

ChassisRigid, cast-aluminum base with opticalmounts bolted directly onto the base providessuperior system stability and reliable results

Detectors Silicon photodiodes forextended wavelengthrange to 1100 nm

Micrometer MonochromatorDrive with Sliding CouplingProvides excellent accuracy, reproducibility and minimizes temperature effects

Slit MechanismPhoto-etched slits for greater accuracy.Stepper-motor driven slit drive with intelligent optimization

Grating 1200 lines/mm, 240 nm blazed, holographic grating, for exceptional stray light performance

Evolution 300Optics

The Evolution 300 optical design includes a long lifetime xenon flash lamp and extended,

wavelength-range silicon photodiode detectors. This system is an excellent choice for the routine

and research laboratories in the Life Science and Pharmaceutical markets.

Evolution 600 Optics

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Light SourcesDeuterium and

Halogen lamps foradded stability

when measuringminute absorbance

changes

MonochromatorBoth models feature

research grade, Ebert monochromator

for excellent energythroughput

Source OpticsAuto-aligningsource optics automatically

optimize throughputwhen the lamp is

changed

Smart SamplingPlug-in, instrument-

controlled accessoriesfor ease of use

and reliability

Detector High-performance,

red-sensitive photomultiplier

(PMT) detector fordemanding samples

ChopperFour-segment chopper runningat 33 Hz eliminates possibleoscillation conflicts with globalmains AC power frequencies of 60 or 50 Hz

Sample Compartment Large sample compartmentand wide beam separation

with interchangeable baseplates for full

sampling versatility

The optical design of the Evolution 600 includes dual deuterium and tungsten sources and a

high-performance photomultiplier detector. These features provide enhanced high-level

performance for the Material Science market and general research applications.

Evolution 300 &Evolution 600 U

V-VisSpectrophotom

eters

Operational Flexibility to Fit Your Lab Needs

The Evolution 300/600 series offers the flexibility to choose a configuration suited to your needs: Local Control, PC software control,or Local Control with PC software control. Each of these configurationsoffers various levels of operational flexibility to fit the individual ormulti-user laboratory environment.

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Local ControlThe Local Control configuration offers complete instrument control, comprehensivemethod and data file manipulation, and savesvaluable bench space. It can also be pass-word protected preventing unwanted accessfrom outside your laboratory. For presentationpurposes, the Local Control screen displaycan be viewed on an external monitor orprojection system using the DVI output.

Local and PC ControlThe Local Control with PC software controlconfiguration gives you the freedom tochoose the configuration that matches yourexperimental needs. For example, use LocalControl to make a quick absorption meas-urement to determine the purity of a DNAsample. You do not need to wait for the PC toboot and the software to load. Later, to runa kinetics experiment based on a methodstored on a network drive, simply openVISION software, and switch the instrumentto PC control. This configuration is ideal forlabs with a variety of experimental needs.

PC ControlThe PC Control configuration allows users tocollect data and distribute it to third-partyapplications for advanced processing andarchival. In multi-user environments, LocalControl with PC software control gives youthe ultimate flexibility.

The PC Software Control configurationuses the powerful VISION suite of softwareto control the Evolution 300/600 instruments.This software allows you to collect, store,recall, and analyze your data, using oneconvenient program. From QC quantitativeanalysis measurements to complex enzymaticassays, the right VISION software packageis available for your application.

The large, color display and tactile keypadmake the Evolution Local Control system convenient and easy-to-use.

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Smart Start SmartStart™ allows each user to set up theSmartStart menu with the applications they usemost often. This increases the throughput of everyuser by eliminating the need to navigate menusand by decreasing the amount of time required totrain users. Load the SmartStart menu with anyapplication, including your stored methods, andwatch the productivity of your lab increase.

The standard VISIONpro software offers advanced scanning, multiple fixed wavelength measurements,and multi-wavelength measurements with customized UVcalc. Quantitative analysis and multicomponentanalysis applications are also included.

VISION Software SuiteVISIONpro™ is a flexible software packagedesigned for the general research and QClaboratory. When used with VISIONpro, theVISIONlife™ software package adds the abilityto monitor and calculate reaction kineticsand to perform DNA melting experiments.For those laboratories requiring 21 CFR Part11 compliance, VISIONsecurity ™ sets thestandard for UV-Vis instrumentation.

Additional application software packages for the Evolution 300/600 UV-Visseries include: VISION Chroma for color measurements and EnzLab for EnzymaticFood Analysis.

VISION Software Application Comparison Chart

Software Scanning Fixed Abs/%T Quant Multicomponent Rate DNA

VISIONpro ✔ ✔ ✔ ✔

VISIONpro + VISIONlife ✔ ✔ ✔ ✔ ✔ ✔

VISIONsecurity ✔ ✔ ✔ ✔

VISIONsecurity + VISIONlife ✔ ✔ ✔ ✔ ✔ ✔

Customized ReportingThe Evolution advanced Report Composer inVISIONpro, VISIONlife and VISIONsecurity software allows you to precisely configure theformat of reports. These templates can be linked toan application method and used to automaticallyproduce reports in your customized format.

Nucleic Acid TestsThe Evolution series provides all the necessarytests for determining DNA purity in thepresence of protein or phenol. DNA/RNAconcentrations can be determined by simpleratios or by wavelength scanning. Thewavelength scanning option allows theadded flexibility of checking for potentialcontaminations.

Various quantization methods are available to determine the concentration ofpurified double-stranded and single-strandedDNA and RNA. Based on user-entered factors,the Evolution instrument measures theabsorbance of the DNA or RNA and calcu-lates the appropriate concentration.

The Oligo (Calc Factor) function allowsyou to enter a specific oligonucleotidesequence and from a subsequent absorbancemeasurement, the system calculates the%GC content, MW of the oligo, molar(pmol/ul) concentration of the sample andan estimate of the Tm value.

Evolution 300 & Evolution 600

UV-Vis Spectrophotom

eters

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With no external circulating water required, the Evolution spectrophotometerwith the Smart Peltier accessory can reach a maximum temperature of 110 °C. This extended range provides added flexibility for making accurate Tm measurements on very long oligonucleotides.

Protein ConcentrationIf your research involves the determination ofprotein concentration assays, the EvolutionLocal Control systems provide the standardprotein assays at the push of a button.

Thermal Denaturation/RenaturationWhether your research examines short orlong DNA or RNA sequences, duplexes ortriplexes, the Evolution VISIONlife ThermalDenaturation/Renaturation system will meetall of your experimental needs. The systemprovides all the tools necessary to controlcomplex heating and cooling profiles withuser defined hold times and ramp ratesdown to 0.1 °C/min. Multiple built-in fittingalgorithms are available to calculate the Tm

value from all types of melting curves.

Comprehensive Solutions for Life Science Biochemical Applications

All of the major, industry-standard protein concentration assays at your fingertips.

The Evolution 300/600 series is the natural fit for any multi-user, life-scienceresearch laboratory. For the user performing fast or routine assays, such asNucleic Acid tests, to users performing more advanced studies, such asMichaelis-Menton kinetics or Thermal Denaturation/Renaturation assays,these instruments offer a configuration ideal for your laboratory.

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KineticsBoth the Local Control and PC Control configurationscater to the user performing single or multi-celldouble-beam kinetics. The Local Control colorscreen makes it easy to distinguish the individualresults from multiple kinetics reactions. Up toseven active reference cuvettes can be used in aseven-sample/seven-reference configuration. Forlaboratories performing more complex assays, suchas inhibitor studies, VISIONlife software providescalculations for the Michaelis-Menton Km and Vmax

parameters using methods such as Lineweaver-Burke and Eadie-Hoftsee.

All of the routine biological assays, including abuilt-in Oligo calculator, are accessible in onlytwo keystrokes.

Quantitative Analysis Made SimpleThe Evolution Quant application is availablein both the Local Control and PC Controlconfigurations. For very quick and simplequant analysis in a routine QC laboratory,the Local Control configuration with built-in,soft-key menu operation is an excellentchoice. In a regulated environment, theEvolution series provides the tools toachieve 21 CFR Part 11 compliance.

The Quant application can use the datafrom up to 50 standards (3 replicates each)to analyze 999 samples (3 replicates each).VISIONpro software provides automatedweight and volume correction eliminatingadditional time-consuming calculations. Ifyour laboratory is performing analysis onmixtures, then the multicomponent analysisapplication, standard with VISIONpro, is ideal.

UVcalc for Automated UserCalculationsIf your analyses routinely demand automatedcalculations on experimental data, you will find the easy-to-use spreadsheet format of the UVcalc function beneficial.UVcalc allows you to enter and store the calculation formulas as you develop dataacquisition methods. When the method isrecalled, both the data acquisition and thecalculations are performed automatically,saving valuable time.

Evolution 300 & Evolution 600

UV-Vis Spectrophotom

eters

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Complete Tools for Pharmaceutical & QC Analysis

Remote Fiber Optics MeasurementsA remote fiber optic probe allows the user to perform absorption measurements inside almost any vessel. A simplerinse between each measurement makes sample carryovernonexistent. Taking the light to any sample container insteadof wasting time bringing the sample to the instrument in acuvette or test tube, increases sample throughput.

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The US FDA Regulation 21 CFR Part 11describes the criteria under which the FDAwill consider electronic records equivalentto paper records and electronic signaturesequivalent to handwritten signatures. Thisregulation applies to all GxP manufacturersand industries that are FDA-regulated.

VISIONsecurity software provides allthe tools necessary to help your laboratoryachieve 21 CFR Part 11 compliance.

Whether compliance requires a manuallyinserted, traceable standard or fully automatedintelligent verification, the Thermo Scientificproprietary Smart Calibration ValidationCarousel (CVC) traceable to NIST1 or NPL2

standards fully automates testing for:• Wavelength accuracy• Photometric accuracy• Stray light• Photometric noise• Photometric stability• Baseline flatness

To obtain the best possible accuracy acrossthe entire wavelength range each instrumentis factory calibrated at multiple wavelengthsfrom the ultraviolet to the near-IR region usinga mercury lamp. An optional mercury lampaccessory is available and user installable.

1. National Institute of Standards and Technology, USA2. National Physical Laboratory, UK

Tablet DissolutionAn important application where complianceissues are critical is in the testing of soliddosage forms. Thermo Fisher Scientific andSOTAX have entered into a relationship toprovide semi-automated and fully automatedTablet Dissolution testing systems supportingApparatus I, II, IV, V and VI. The systemcombination provides a complete and seam-less solution for any dissolution laboratory.

Evolution 300 with the SOTAX Apparatus IV flow-through system offers a unique solution fora variety of Tablet Dissolution assays

With continually changing requirements to keepyour Evolution spectrophotometer up to regulatorystandards, no supplier in the industry can providea more comprehensive selection of calibrationand verification tools.

VISIONsecurity not only has all of the acquisition,analysis, and reporting capabilities found in theVISIONpro software, but also has the tools necessary to control individual or group access to the instrument, data, and records. Userauthentication at start-up allows event audittrails, electronic signatures, and prevents usewhile operator is away.

By replacing paper-based archival storage withelectronic data storage and data transfer, theproper use of VISIONsecurity software with your Thermo Scientific spectrophotometer willhelp you pass FDA audits while reducing costsand increasing security.

Comprehensive Solutions for Regulatory Compliance

Evolution 300 & Evolution 600

UV-Vis Spectrophotom

eters

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VN Absolute Specular ReflectanceAccessoryThe VN accessory offers significant designadvantages over other designs such as rela-tive specular and VW specular reflectanceaccessories. With relative designs, knowncalibrated specular standards, such as afirst-surface aluminum mirror, are required.These designs require finding a suitablestandard and maintaining the quality of thefront surface. VN has an advantage overVW designs because the light beam onlystrikes the sample surface once. The “W”configuration means the sample strikes thesample surface twice. With the innovativeVN accessory, it is easier than ever tomeasure small samples.

Diffuse Reflectance AccessoryThe Diffuse Reflectance accessory opens thedoor to measuring many samples that werealmost impossible to measure in the past.This accessory offers a horizontal mountingreflectance port to conveniently measureboth delicate and large samples. The doublebeam Spectralon® integrating sphere ensuresthe highest possible energy throughput andaccuracy. A transmittance port is included fordiffuse transmittance measurement of solidor turbid liquid materials. The integratingsphere is fully CIE compliant for performingcolor measurements.

Solid Sample Holder AccessoryThe Solid Sample Holder provides thecapacity to measure a variety of solid samples in % Transmission mode. Anassortment of sample holders are availablefor round, square and odd shaped samples.The 2"x 3" sample slides allow other industry accessories, such as a relativespecular reflectance accessory, to be usedat various angles of incidence.

High-performance Measurements for Material Science

The Evolution 300/600 UV-Vis spectrophotometers offer a variety of accessories designed to meet your solid sample testing needs. With thehorizontal mounting of our Diffuse Reflectance accessory (DRA) or our single-bounce VN Absolute Specular Reflectance accessory, a new world ofmaterial science measurements is available with the Evolution spectropho-tometers. Whatever your routine high-performance measurements require,we have a variety of sample handling accessories catering to your needs.

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The horizontal sample port of the DRAprovides almost unlimited sample sizemeasurements. Diffuse Reflectance samples using the DRA in combinationwith the VISIONlite ColorCalc softwareprovides routine solutions for CIE Colorand APHA Color compliant measurements.

The VN accessory can easilymeasure sample sizes down to0.5" (12.5 mm) diameter with anangle of incidence of 10°

The VISIONlite MaterialsCalc software provides an array ofcalculations ranging from Luminous Transmittance, Haze,

UV protection and UPF protection of Fabrics. One of the keycalculations is the luminous transmittance of sunglasses.

An example of a pair of high end sunglasses is shown herealong with the luminous transmittance calculation.

The Evolution 300/600 UV-Vis series providesSmart Accessories with auto-intelligence. BothLocal Control and PC Control configurationsrecognize individual accessories and auto-matically identify the accessory’s uniqueserial number. This saves time and enhancesproductivity in regulatory environments.Kinematic mounts allow easy interchangeand reproducible alignment each time theaccessory is removed and installed.

Smart Accessories intelligently interactwith software menus and local control.Simply snap the accessory in place and theappropriate software menu for that accessorywill appear on the screen. All other accessorysoftware menus are hidden eliminating possible confusion.

Evolution 300 & Evolution 600

UV-Vis Spectrophotom

eters

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Smart Accessories for Enhanced Productivity

Smart Calibration Validation Carousel (CVC)Cylindrical Cell Holder

VN Absolute Specular Reflectance Accessory

Smart Thermostatted Linear 8-Cell Changer

Adjustable Microcell Holder

Praying Mantis Accessory

Smart Thermostatted Rotary 7-Cell Changer Smart Peltier Thermostatted Single Cell Holder

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Smart Sipper Accessory

Test Tube and Vial Holder

VERSA Fiber Optic Probe

Long Pathlength Rectangular Cell Holder

15°, 30°, 45°, and 60° Specular Reflectance Accessories

Solid Sample HolderDRA-EV-600 Diffuse

Reflectance Accessory

Temperature Probe

RX2000 Rapid Mix Accessory

nanoCellAccessory

Tap our expertise throughout the life of your instrument. Thermo Scientific Services

extends its support throughout our worldwide network of highly trained and certified

engineers who are experts in laboratory technologies and applications. Put our team

of experts to work for you in a range of disciplines – from system installation, training

and technical support, to complete asset management and regulatory compliance

consulting. Improve your productivity and lower the cost of instrument ownership

through our product support services. Maximize uptime while eliminating the

uncontrollable cost of unplanned maintenance and repairs. When it’s time to

enhance your system, we also offer certified parts and a range of accessories and

consumables suited to your application.

To learn more about our products and comprehensive service offerings,

visit us at www.thermo.com.

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