Eve PJ4 Figure 1: evePJ4 behaves as a hypomorphic allele
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Figure 1: evePJ4 behaves as a hypomorphic allele. Comparison of wingless protein expression from immunofluorescent microscopy. Anti-wingless antibody was used to probe for wingless protein (wg) expression. eve 3 is a known amorph resulting in a loss of wg expression. eve PJ4 exhibits loss of wg patterning resembling the eve 3 amorphic phenotype; however wg is still produced. wg NE2 eve PJ4 exhibits wg patterning that more resembles the wild-type phenotype. Embryos are orientated with anterior to left and dorsal up. (Ko, M., Lau, J., and Mendoza-Elias, J. Bio205L September 2006) Stage 9 WT eve 3 eve PJ4 wg NE2 eve PJ4 Stage 10 Stage 11
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Figure of 4 representative genotypes at 3 developmental stages (Bownes Stages): WT (control), eve3 (null mutant), evePJ4, evePJ4wgNE2.
Transcript of Eve PJ4 Figure 1: evePJ4 behaves as a hypomorphic allele
Figure 1: evePJ4 behaves as a hypomorphic allele. Comparison of wingless protein expression from immunofluorescent microscopy. Anti-wingless antibody was used to probe for wingless protein (wg) expression. eve3 is a known amorph resulting in a loss of wg expression. evePJ4 exhibits loss of wg patterning resembling the eve3 amorphic phenotype; however wg is still produced. wgNE2evePJ4 exhibits wg patterning that more resembles the wild-type phenotype. Embryos are orientated with anterior to left and dorsal up. (Ko, M., Lau, J., and Mendoza-Elias, J. Bio205L September 2006)
Stage 9
WT
eve3
evePJ4
wgNE2evePJ4
Stage 10 Stage 11