European Union Reference Laboratory for GM Food & Feed - The...
Transcript of European Union Reference Laboratory for GM Food & Feed - The...
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The state of GMO analysis
in Africa
including regional networks
Chris Viljoen
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Basic Needs and Gaps
for GM detection in Africa
No technical support for GM detection
No mechanism for cooperation between GM detection laboratories
No guidelines for best practice / minimum criteria
No mechanism to facilitate training
No proficiency appraisal of laboratories
No links with other Networks
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Regional GM detection Networks
in Africa
West African informal Network
East African informal Network
Southern African Network
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Participating Countries
• Botswana
• Namibia
• Madagascar
• Malawi
• Mozambique
• Swaziland
• South Africa
• Tanzania
• Zambia
• Zimbabwe
17 laboratories
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SANGL Coordination
• Chris Viljoen (South Africa)
• Dahlia Garwe (Zimbabwe)
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Characteristics of the Network
• Non-political
• Participation is voluntary
• Non-prescriptive
• Participation is endorsed by the managing authority of the laboratory and the National Biosafety Focal Point
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Structure of the Network
• Laboratories are represented equally
• The Network consists of a central coordinating
reference laboratory (HUB) and participating labs
(NODES)
• Laboratories nominate a participant in the
Network
• Decision making is based on consensus
• Network is not limited to but operated with
logistical support from RAEIN-Africa
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1. To build and strengthen capacities for GM
detection
2. To establish guidelines and harmonised GM
detection methods
3. To achieve international recognition in GM
detection
4. To compile technical guidance papers on GM
detection
SANGL Objectives
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5. To establish links with other international GM
detection laboratories and Networks
6. To establish an interactive communication
platform for SANGL laboratories
7. To establish inter-laboratory collaboration
between SANGL laboratories
8. To mobilise resources to support SANGL
activities
SANGL Objectives cont.
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SANGL Activities
• 2009: Workshop to establish SANGL
• 2010: Training workshop in GM detection
• 2011: Strategic planning workshop and training
in quality management
• 2012: 1st Proficiency round
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Status
Level
Functional level of
laboratory (L) Equipment (E)
Quality Management
Documents (Q)
Expertise of
personnel (P)
1 Laboratories that can
perform PCR based GMO
screening and Real-time
PCR GMO quantification
PCR System
Real-Time PCR System
Gel electrophoresis
Gel documentation system
Optional: ELISA Plate reader
Quality system that
includes a quality Manual,
safety manual and standard
operating procedures
Personnel are trained
in PCR based GMO
screening and GMO
quantification
2 Laboratories that can
perform PCR based GMO
screening
PCR System
Gel electrophoresis
Gel documentation system
Optional: ELISA plate reader
GMO testing is performed
according to validated
methods
Personnel are trained
in PCR based GMO
screening
3 Laboratories that use strip
and/or ELISA based testing
to detect GMOs
PCR System or ELISA plate
reader
Methods are not validated Personnel are trained
in strip / ELISA
testing
4 Laboratories that are not
able to apply any form of
GMO testing
No specialized equipment No methods Personnel have no
training
Mapping the status
of SANGL laboratories
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Summary of functional status
of SANGL laboratories
L: Functional level of lab
E: Equipment
Q: Quality management
P: Expertise of personnel
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Summary of functional status
of SANGL laboratories
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Summary of functional status
of SANGL laboratories
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Summary of functional status
of SANGL laboratories
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Summary of functional status
of SANGL laboratories
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Summary of functional status
of SANGL laboratories
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SANGL laboratories are at different levels of progress
Challenge: To provide different levels of support
There is a continued need to support the development of expertise in GM detection
Conclusions
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Mobilise resources to continue to maintain SANGL
Mobilise resources to support SANGL activities
Challenges
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Training Workshop
in GM Detection
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International Workshop of GMO-analysis networking
8 – 9 April 2013
JRC Ispra (VA) Italy
Regional meetings – Region AFRICA
Discussion point:
1. Forthcoming regional technical challenges for GMO analysis
Outcome of the meeting: • Different laboratories are at different levels in terms of capability, infrastructure
and expertise • Need for regional capacity building in GM detection • Need for infrastructure development in some countries • Lack of commercial product support and access to equipment and reagents in some
countries • Lack of a mandate to perform GM detection in terms of regulatory frameworks in
some countries
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International Workshop of GMO-analysis networking
8 – 9 April 2013
JRC Ispra (VA) Italy Regional meetings – Region
Discussion point:
2. Regional needs to meet these challenges
Outcome of the meeting: • Infrastructure development where necessary • Capacity building:
- GM detection - Lab management and quality management
• Need to develop regional Networks to support GM detection capacity building initiatives
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International Workshop of GMO-analysis networking
8 – 9 April 2013
JRC Ispra (VA) Italy Regional meetings – Region
Discussion point:
3. Road map / Prioritisation of necessary actions
Outcome of the meeting: • Actions that need to be prioritized at regional level:
- Political awareness development - Mapping of labs involved in GM detection in terms of capacity, infrastructure,
expertise and capacity building needs - Developing regional networks through capacity building
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International Workshop of GMO-analysis networking
8 – 9 April 2013
JRC Ispra (VA) Italy Regional meetings – Region
Discussion point:
4. Next steps at regional level / training needs that JRC could help to meet
Outcome of the meeting:
• Continue with “Train the trainer” approach • Training depending on level of expertise of the lab:
- Sampling, DNA extraction, GM detection, GM quantification, data management, lab management and quality management
• Follow-up training with proficiency trials targeted to the level that would be relevant to that region/group of labs
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International Workshop of GMO-analysis networking
8 – 9 April 2013
JRC Ispra (VA) Italy
Regional meetings – Region
Discussion point:
5. Identification of up to 3 regional representative(s) as contact person(s)
Outcome of the meeting: (4 regions identified) • West Africa: Ousmane Koita (Mali) • East Africa: Joyce Malinga (Kenya) • Southern Africa: Chris Viljoen (South Africa) • Central Africa: Roger Darmam (Cameroon) • African Union as cc in communication