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![Page 1: Establishment and evaluation of garlic collection from Central Asia for fertility potential and organo-S compounds EU project “Garlic and Health” - Work.](https://reader036.fdocuments.net/reader036/viewer/2022062500/56649e415503460f94b32daa/html5/thumbnails/1.jpg)
Establishment and evaluation of garlic collection
from Central Asia
for fertility potential and organo-S compounds
EU project “Garlic and Health” - Work Package 1Israel: Rina Kamenetsky and Haim D. Rabinowitch
Idit London, Marina Baizerman, Ada Harazy and Amit Shiftan
The Netherlands: Chris Kik, A.W. van Heusden, R. Vrielink-van
Ginkel and K. Burger-Meijer
Uzbekistan: Furkat Khassanov and Habibullo Shomuradov
France: Jacques Auger and Ingrid Arnault
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Workpackage1: Genetic Resources
Objectives• Construct a large collection of garlic landraces and of
close wild relatives, in order to expand the available diversity;
• Construction and maintenance of a core collection; • Screening for fertility;
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Workpackage1: Genetic Resources
Objective 1Construct a large collection of garlic landraces and of close wild relatives, in order to expand the available diversity. Israel: Rina Kamenetsky and Haim D. Rabinowitch
Idit London, Marina Baizerman, Ada Harazy and Amit ShiftanUzbekistan: Furkat Khassanov
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Rationale
Biochemical and molecular studies suggest that the highest level of heterogeneity prevails within the Central Asian cultivar group (Hong, 1999; Maaβ & Klaas, 1993; Pooler &
Simon, 1995), which could serve as the most important source for garlic genetic variation and improvement (Etoh & Simon, 2002; Kamenetsky
et al., 2003a; 2003b).
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Collection periods and number of accessions
Garlic entries in the Israeli collection
Sources Year No. accessions
Previous collections 1996-1999 ~100
1st G&H collection 2000 120
2nd G&H collection 2001 121
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Garlic living collection in Israel
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Sharing and distribution of genetic material for further use in the project
• 2000: Bulbs of 115 accessions sent from Israel to C. Kik (P1; The Netherlands) for phenological evaluation and DNA typing.
• 2000: Bulbs of 10 accessions were sent from Israel to J. Auger (P5; France) for CSO analysis
• 2000: Bulbs sent from Israel to Veronique Chovelon (P9 France) for generation of virus free propagules.
• 2002: Bulbs of 32 accessions sent from Israel to J. Auger (P5; France) for CSO analysis.
• 2002: Seed of two garlic accessions sent from Israel to Meriel Jones (P3, UK), for biochemical analysis of seedlings.
• 2002: Phytotron raised seedlings sent from Israel to Meriel Jones (P3, UK), for biochemical analysis.
• 2002: Bulbs of 53 accessions sent from The Netherlands to J. Auger (P5; France), for CSO analysis.
• 2003: Samples sent from Israel to Rolf Gebhardt (P11, Germany) for experimentation.
Constructio
n of a la
rge collection
Mission co
mpleted
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Workpackage1: Genetic Resources
Objective 2
Construction and maintenance of a core collection;
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Evaluation of garlic collections from Central Asia
for fertility potential and other economic traits
EU project “Garlic and Health”
Israel: Rina Kamenetsky and Haim D. Rabinowitch
Idit London, Marina Baizerman, Ada Harazy and Amit Shiftan
The Netherlands: Chris Kik, A.W. van Heusden, R. Vrielink-van
Ginkel and K. Burger-Meijer
Uzbekistan: Furkat Khassanov and Habibullo Shomuradov
France: Jacques Auger and Ingrid Arnault
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Recent studies indicate that garlic species complex consists of five groups (Fritsch and Friesen, 2002):
•Sativum
•Longicuspis
•Ophioscorodon
•Subtropical
•Pekinense
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subtropical subgroup
Ophioscordongroup
subgroup Pekinense
Sativum group
Intraspecific classification of garlic, three major groups and two
subgroups were proposed (Fritsch and Friesen, 2002)
Longicuspis group
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Evaluation
Geography Phenology
DW andPA Content Morphology
Fertility & Fertility Restoration
Collecting in Central Asia
Israel The Netherlands
Evaluation
DNA Phenology
Biochemical analyses - France
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Missions to Central Asia in 2000 and 2001, collected 241 accessions, including wild populations and land-races.
Garlic clones vary in most vegetative characteristics (leaf number, bulb size and structure), as well as in floral scape elongation and inflorescence development.
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Garlic clones are classified according to their ability to bolt:
1. Complete bolting: long stalk with many flowers and topsets
2. Incomplete bolting: thin short stalk with a few large topsets
3. Nonbolting: producing only cloves inside incomplete scape
(Takagi, 1990)
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Incomplete-bolting accession
Bolting accession
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Missions to Central Asia in 200 and 2001, resulted in a collection of 241 accessions, including wild populations and land-races.
Garlic clones vary in most vegetative characteristics (leaf number, bulb size and structure), as well as in floral scape elongation and inflorescence development.
Genetic fingerprinting by means of AFLP markers revealed three distinct groups within this collection, differing in flowering ability and organo-S content (Chris Kik and co-workers)
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AFLP fingerprinting of garlic
collections
in Wageningen and Gatersleben,
Germany
9707697078
99005
9900699007
99008
9906699067
99068
99069
97015
9707697078
99005
9900699007
99008
9906699067
99068
99069
97015
1 1 1 1 1 1 0 1 1 1 1
0 0 0 0 0 0 1 0 0 0 00 0 0 0 0 0 0 1 1 1 0
1 1 1 0 1 0 1 1 1 1 1
1 1 1 0 0 0 1 0 0 0 1
Part of AFLP-fingerprint pattern for 13 garlic accessions
(Chris Kik et al., G&H annual report, 2000)
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Based on AFLP fingerprinting and one enzyme primer combination, the collection was subdivided into three distinct groups: Sativum, Longicuspis A and B.
The groups differed considerably in AFLP variation: The variation within the Sativum group was significantly low compared to that in the Longicuspis groups.
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76%
15%
9%
AFLP fingerprinting assisted classification and separation of garlic accessions collected in Central Asia in 2000 (n=120).Chris Kik, A.W van Heusden, R Vrielink-van Ginkel and K. Burger-Meijer
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Recent studies indicate that garlic species complex consists of five groups (Fritsch and Friesen, 2002):
•Sativum
•Longicuspis
•Ophioscorodon
•Subtropical
•Pekinense
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Missions to Central Asia in 200 and 2001, resulted in a collection of 241 accessions, including wild populations and land-races.
Garlic clones vary in most vegetative characteristics (leaf number, bulb size and structure), as well as in floral scape elongation and inflorescence development.
Genetic fingerprinting by means of AFLP markers revealed three distinct groups within this collection, differing in flowering ability and organo-S content.
Field evaluation for flowering and other plant traits revealed distinct differences between populations of incomplete- and bolting plants.
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Leaf Scape Flower/topset Pollen
number length, cm index* germination, %
Early (15/5 - 5/6) 10.6±0.17 61.2±1.8 T 0
Mid (5/6 - 25/6) 13.4±0.46 80.7±2.7 F 94.70
Late (25/6 - 5/7) 15.7±1.02 78.4±6.8 F 100
Date of spathe breaking
Physiological parameters of bolting garlic (42 accessions) under
Israeli environmental conditions
*T = mostly topsets; F = mostly flowers
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Phenotypic variability
• Garlic clones vary in most vegetative characteristics (leaf number, bulb size and structure), as well as in floral scape elongation and inflorescence development. Clear distinction was made between semi-bolting and bolting accessions. Most of the latter accessions produced flowers with fertile pollen and receptive stigma.
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Missions to Central Asia in 200 and 2001, resulted in a collection of 241 accessions, including wild populations and land-races.
Garlic clones vary in most vegetative characteristics (leaf number, bulb size and structure), as well as in floral scape elongation and inflorescence development.
Genetic fingerprinting by means of AFLP markers revealed three distinct groups within this collection, differing in flowering ability and organo-S content.
A clear distinction was made between incomplete bolting and bolting populations;
Wide variations were recorded with regard to differentiation of topsets, their size, number and rapidity of development.
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Inflorescence performance
mainly topset development mainly flower development
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Missions to Central Asia in 200 and 2001, resulted in a collection of 241 accessions, including wild populations and land-races.
Garlic clones vary in most vegetative characteristics (leaf number, bulb size and structure), as well as in floral scape elongation and inflorescence development.
Genetic fingerprinting by means of AFLP markers revealed three distinct groups within this collection, differing in flowering ability and organo-S content.
A clear distinction was made between incomplete bolting and bolting populations;
Wide variations were recorded with regard to differentiation of topsets, their size, number and rapidity of development.
Significant variation in organo-S compounds (alliin, isoalliin, allicin and related dipeptides) was found within garlic collections and between plants grown under differing environmental conditions.
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Raw material: Samples raised in the Netherlands and
Israel.
Procedure: HPLC-UV analyses.
Jacques Auger and Ingrid Arnault
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Individuals (axes F1 and F2 : 84 %)
L/B
-2,5
-2,0
-1,5
-1,0
-0,5
0
0,5
1,0
1,5
2,0
-4 -3 -2 -1 0 1 2 3 4
-- axis F1 (59 %) -->
-- a
xis
F2
(24
%)
-->
L/B
L/B
L/B
L/B
L/A
L/A
L/A
L/A
S
S
S
S
SS
Principal Component Analysis based on the variation of five organo-S compounds for 15 garlic accessions. Bulbs were collected in Central Asia in 2000 and grown in Holland in 2001/2002. Measurements were made in September, on mature, freshly harvested bulbs of the Sativum (S), and Longicuspis A (L/A) and Longicuspis B (L/B) subgoups.
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Effect of origin on organo-S compounds in six garlic accessions grown in parallel in Holland and Israel
Acc. numberalliinGLUALCSIso
GLUALCSallicinisoalliin
Isr. 250533.3125.8521.9016.240.24
Dutch 7309535.667.65 16.9611.640.66
Isr 250746.3023.3224.9616.520.29
Dutch 7309750.866.7313.029.363.23
Isr 251043.9610.6318.5319.201.54
Dutch 7310028.987.3716.4712.160.76
Isr 251742.6821.6424.3924.500.51
Dutch 7310746.956.8716.178.180.68
Isr 243945.2321.7120.9819.951.01
Dutch 7302939.885.3812.447.302.97
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Results:
1) Within the collection, a large quantitative variation in organo-sulphur compounds: alliin, isoalliin, allicin, and related dipeptides was found.
2) Environmental effects were evident.
Jacques Auger and Ingrid Arnault
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Main Goal :
Evaluation of the collected accessions
and construction of the representative
core collection
Evaluatio
n and constructio
n of t
he data
Evaluatio
n and constructio
n of t
he data
base necessary
for c
ore colle
ction
base necessary
for c
ore colle
ction
Mission co
mpleted
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Workpackage1: Genetic Resources
Objective 3
Screening for fertility;
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Flowering performance of the garlic collection-2000 in the 2001 and 2002 seasons
27
34
56
4
25
37
49
10
0
20
40
60
80
100
2001 2002
Dead*
Non bolters
Semi bolters
Bolters T>F
Bolters F>T
*Under Israeli conditions plants did not bulb and died at the end of the season T = mostly topsets; F = mostly flowers
%
Growing season
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Publications:
• Kamenetsky R, London Shafir I., Baizerman M, Khassanov F, Kik C. and H.D. Rabinowitch Garlic (Allium sativum L.) and its wild relatives from Central Asia: evaluation for fertility potential . XXVIth International Horticultural Congress, Toronto, Canada (In press).
• Kamenetsky R, London Shafir I., Khassanov F, Kik C, J. Auger and H.D. Rabinowitch
Garlic from Central Asia: genetic resources and fertility potential. 4th International Symposium on Edible Alliaceae (to be presented in China)
• Kamenetsky, R., London Shafir, I., Khassanov, F., Kik, C., van Heusden, A.W., Vrielink-van Ginkel, M., Burger-Meijer, K., Auger, J., Arnault, I. and H.D. RabinowitchDiversity in fertility potential and organo-sulphur compounds among garlics from Central Asia. J. Biodiversity Conservation (In press).