Epithelium and Junctions

VIBS 443/602

EM 3: region of basal lamina and

connective tissue beneath intestinal

epithelial absorptive cells

1. Plasma membrane

2. Basal lamina

3. Connective tissue

EM 17; capillary in the lamina

propria of the intestine

(duodenum); 13,500x

1. Smooth muscle cell

2. Capillary

3. Endothelial cell

Renin granules in Kidney (PAS)

258

Ileum, monkey (PAS)

249

Trachea, monkey 133

Esophagus and trachea, monkey –

glands in trachea

242

Larynx (Gallego's stain) 429

429

SPECIALIZATION OF EPITHELIA

MAINTAIN EXTENSIVE

CONTACTS AMONG CELLS

STRUCTURALLY AND

FUNCTIONALLY POLARIZED

JUNCTIONS

ZONULA OCCLUDENS - TIGHT

JUNCTION (BELT)

ZONULA ADHERENS –

ADHERING BELT

DESMOSOME (MACULA

ADHERENS) - SPOT ATTACHMENT

GAP JUNCTIONS - COMMUNICATION

Terminal bars

32409

EM 6b: Region showing zonula

occludens, zonula adherents,

and macula adherents junctions

between cells.

1. Zonula occludens

2. Zonula adherens

3. Desmosome EM 6b

EM 6b

EM 6c

EM 4: Continuity between junctional

complex and terminal web

1. Terminal web

2. Interdigitation of plasma

membrane

3. Terminal bar

EM 4a: Ridges and grooves of

zonula occludens visualized

by freeze cleave microscopy

1. Zonula occludens

Inside the cell Outside the cell

P = Protoplasmic side E = Ectoplasmic side

EM 4a

EM 2a: Particles and pits of gap

junction visualized by freeze

cleave microscopy

1. Gap junction

EM 2a

Triad with bile duct Liver & spleen with

colloidal carbon, rat

118

EM 2: portion of two cells including

gap junction

1. Desmosome

2. Gap junction

3. Rough endoplasmic reticulum

absorptive cells of Ileum, monkey

250

Ileum, monkey (PAS)

249

argentaffin cells of Ileum, monkey

250

Large intestine or Colon, monkey 153

EM 4c: intestinal absorptive cell

(super nuclear region); 60,000x

1. Budding RER

2. Coated vesicle

3. Golgi

4. Mitochondria

5. Nucleus

6. Plasma membrane

7. Primary lysosome

Endothelial cells lining small blood vessel lumen with smooth muscle cells

and fibrolasts in the vessel wall.

1. Lumen

2. Endothelial cell

3. Fibrolast

EM 10a: Endothelial cells lining

capillary with pericyte in the vessel

wall.

1. Lumen

2. Endothelial cell

3. Tight junction

Skeletal muscle in spermatic cord

UT 196

196

UT196 valves in large vein of

spermatic cord

34456

EM 10f: arteriolar wall; 30,000x

1. Apical caeolae

2. Collagen

3. Dense bodies

4. Elastic fiber

5. Endothelial cell

6. Nuclear pore

7. RER

EM 8h: macrophage in testis; 30,000x.

1. Enlarged basal lamina

2. Heterophagic vacuole

3. Leydig cell cytoplasm

4. Nucleolus

5. Myoid cell

Skin abdomen 206

Skin, scalp 209

Skin hand monkey 109

Skin hand monkey 109

Skin 111

Skin, foot 408

Vagina

178

109

EM 8g: epidermis; 7,000x. Note

desmosomal connections

between cells

1. Keratinocyte

2. Desmosome

3. Melanocyte

EM 16: surface mucosa of stomach;

13,500x

1. Granules of surface mucous cells

2. Mitochondria

3. Nuclei

4. Lumen

Urinary bladder, monkey

160

Ureter – transitional epithelium

and smooth muscle

262

Penis – transitional epithelium and

surrounding spongy cavernous of penal urethra

277

Penis - transitional epithelium of penal urethra 277