Epidermal papilloma in a gold spot plecostomus (Pterygoplichthys joselimaianus Weber, 1991)

H. Rahmati-holasoo1*, S. Shokrpoor2, H. A. Ebrahimzadeh Mousavi1 and M. Ahmadpoor1

1Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran; 2Department of Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Abstract-

tomus Pterygoplichthys joselimaianus

P. joselimaianus.

* Corresponding author’s e-mail: rahmatih@ut.ac.ir

Introduction

epidermal papillomas (Peters and Watermann,

virus-induced neoplastic skin disease, occur-

Hippoglossoides elas-sodonobscuracultured Atlantic salmon Salmo salar L. (Carlisle,

(Mustelus canisbrown bullhead (Ameiurus nebulosus), white sucker (Catostomus commersoni) (Sonstegard,

Ameiurus melas) (Grizzle (Osmerus eperlanus(Cyprinus carpio) (Hedrick et al., 1990; Calle et al.,

1999), cultured turbot (Scophthalmus maximus) (Lamas et al., 1990), rainbow smelt (Osmerus mordax mordax) Kuroga-rei (Pleuronectes obscuresand Atlantic halibut, (Hippoglossus hippoglossus)

epidermal papilloma is reported in a gold spot plecostomus (Pterygoplichthys joselimaianus).

Materials and methodsIn January 2014, an indoor gold spot plecosto-mus (P. joselimaianus Weber, 1991) was presented

-lomatous growth protruding on the lip (Figure

2-1

The diagnosis was established by histological

-

-

stained with haematoxylin-eosin (H&E).

Results

with papillary projections on connective tissue (Figure 2). Epidermal cells were tightly packed, disorganized and had eosinophilic cytoplasm and large, round to oval nuclei. Additionally, individual cells in the hyperplastic epithelium were swollen with a clear cytoplasm and large, round to oval vesicular nucleus (koilocytes)

individual cell necrosis was common (Figure

nucleus but margination at the nuclear mem-brane was evident (Figure 4). As such, we clas-

Histologically, internal organs such as liver, kidney and spleen were normal.

DiscussionIn many cases, virus or virus-like particles

However, oncogenicity correlation has been -

Anders and Yoshimizu, 1994; Calle et al., 1999). In contrast, viral agents have not been observed

Premdas

Figure 1. Gold spot plecostomus (P. joselimaianus) with

Bull. Eur. Ass. Fish Pathol., 34(4) 2014, 119

Figure 2.

Figure 3.

120, Bull. Eur. Ass. Fish Pathol., 34(4) 2014

that chemical and biotic pollutants (Sonstegard,

-

1992; Kortet et al., 2002), crowding (Premdas -

(Premdas et al., 2001), sex steroid injections in white sucker (Catostomus commersoni) (Premdas

-one in roach (Rutilus rutilus) (Kortet et al., 2003)

- (P. joselima-

ianus

suckers by contrast brown bullheads had only a

in their oral cavity, these lesions were somewhat intermediate between the plaques and papil-

lip tumours and both plaques and papillomas

Lesions observed in this study have similari-ties to those described by Hedrick et al., (1990). However, those authors observed herpesvirus-

Cyprinus carpio) by electron microscopy which we did

Figure 4. Nuclei with marginated chromatin (arrowheads) at the nuclear membrane are evident (H&E).

Bull. Eur. Ass. Fish Pathol., 34(4) 2014, 121

not undertake in the present study. Similarly, Mustelus

canis) papilloma epidermal cells revealed no particles or structures that might be construed as viral. However, it should be pointed out

-malin and re-embedded in Epon (Wolke and

liver neoplasia and papillomas observed in

-plasms. However, in this study, chemical and biotic pollutants, environmental stress and crowding were absent and no histologic ab-normalities were observed in the liver. Histo-logically the lesion described in this study is

AcknowledgementsR. Toyneghli, F. Amiri and A. Baghdadi are

References

an epizootic epidermal papillomatosis in European smelt (Osmerus eperlanus). In “Viruses of lower vertebrates” (W. Ahne

Berlin.

Diseases of Aquatic Organisms 19

environmental carcinogenesis in Niagara Journal of great Lakes Research

9

Hazard Assessment of Chemicals 3

BS (1991). Epizootic papillomas in brown bullheads Ictalurus nebulosusStream Reservoir, New York. Diseases of Aquatic Organisms 11

Observations on epidermal papillomata in wild and cultured Atlantic salmon Salmo salar L. in Finland. Journal of Fish Diseases 3

Calle PP, McNamara T and Kress Y (1999). Herpesvirus-associated papillomas in koi carp, Cyprinus carpio. Journal of Zoo and Wildlife Medicine 30

Salmo salar). A

Stirlmg, Stirling.

Fujimoto Y, Madarame H, Yoshida H, Moriguchi R, Kodama H and Iizawa H

obscura). Japanese Journal of Veterinary Research 34

Journal of Aquatic Animal Health 10, 191-201.

Journal of the National Cancer Institute 73, 1133-1142.

Ictalurus melas sewage pond. Journal of Fish Diseases 4,

122, Bull. Eur. Ass. Fish Pathol., 34(4) 2014

Hayes MA, Smith IR, Rushmore TH, Crane TL, Thorn C, Kocal TE and Ferguson HW (1990).

areas in Lake Ontario. The Science of the Total Environment 94

Hedrick RP, “Viruses of lower vertebrates”

Springer-Verlag, Berlin, Federal Republic

McDowell TS (1990). Herpes viruses

koi Carp (Cyprinus carpio). Journal of Wildlife Diseases 26,

associated virus. Journal of Wildlife Diseases 33

Korkea-aho TL, Partanen JM, Kukkonen JVK

roach Rutilus rutilus. Diseases of Aquatic Organisms 78

Korkea-aho TL, Partanen JM, Kiviniemi

Association between environmental stress

Rutilus rutilus. Diseases of Aquatic Organisms 72

Kortet R, Vainikka A and Taskinen J (2002) Epizootic cutaneous papillomatosis in roach (Rutilus rutilus): sex and size dependence, seasonal occurrence and between

Diseases of Aquatic Organisms 52

Kortet R, Vainikka A, Rantala MJ, Jokinen I and Taskinen J (2003). Sexual ornamentation, androgens and papillomatosis in male roach (Rutilus rutilus). Evolutionary Ecology Research 5, 411-419.

Lamas J, Anadon R, Devesa S and Toranzo AE (1990). Visceral neoplasia and epidermal papillomas in cultured turbot Scophthalmus maximus. Diseases of Aquatic Organisms 8,

spawning papillomatosis in smelt, Osmerus eperlanus L., in the River Thames. Journal of Fish Biology 40

common dab, Limanda limanda. Diseases of Aquatic Organisms 22, 101-114.

papilloma and skin ulcers in common dab Limanda limanda Denmark. Diseases of Aquatic Organisms 30,

Hippoglossus hippoglossus (L.). Journal of Fish Diseases 30,

epidermal tumors in the European eel, Anguilla anguilla L. Archiv für Fischereiwissenschaft 27

epidermal papillomas on the European eel, Anguilla anguilla L. Journal of Fish Diseases 2

causes. Marine Ecology 1

bullheads Ictalurus nebulosus. Diseases of Aquatic Organisms 24

(1994). Naturally occurring orocutaneous

bullheads (Ictalurus nebulosus) in New York State. Veterinary Pathology 31

Bull. Eur. Ass. Fish Pathol., 34(4) 2014, 123

suckers (Catostomus commersoni) held in the laboratory. Environmental Biology of Fishes 40

papillomata in Catostomus commersoni. Journal of Fish Biology 59

in white sucker (Catostomus commersoni)

Journal of Great Lakes Research 21

juvenile Atlantic salmon, Salmo salar L., in New Brunswick, Canada. Journal of Wildlife Diseases 21

Sano T, Morita N, Shima, N and Akimoto M (1991). Herpesvirus cyprini: lethality and oncogenity. Journal of Fish Diseases 14

contaminants; an epidemiological approach. Ontario Ministry of the Environment Technology Transfer Conference 6

Smith IR, Baker KW, Ferguson HW and Hayes

Catostomus commersoni. Diseases of Aquatic Organisms 6

Smith IR, Ferguson HW and Hayes MA

bullhead, Ictalurus nebulosus (Lesueur), and white sucker, Catastomus commersoni

Canada. Journal of Fish Diseases 12

Annals of the New York Academy of Science 298,

York State. Toxicologic Pathology 23

tumors in the black plaice Pleuronectes obscurus Russian Journal of Marine Biology l23

Wellings SR, Chuinard RG, Gourley RT and

hippoglossoides elassodon,

neoplasms in other pleuronectids. Journal of the National Cancer Institute 33, 991-1004.

Mustelus canis. Journal of Wildlife Diseases 12,