Enzymes for Environmental Sustainability and Maximizing Profits · 1.0% 1.5% 2.0% 2.5% 3.0% Yield...
Transcript of Enzymes for Environmental Sustainability and Maximizing Profits · 1.0% 1.5% 2.0% 2.5% 3.0% Yield...
Enzymes for Environmental Sustainability and Maximizing Profits
For more than ten years, Novozymes has been recognized as one of the most sustainable companies in the world by the Dow Jones Sustainability Index (DJSI), which evaluates and ranks leading companies based on their sustainability performance.
Sustainability – Novozymes commitment
Agenda
1. NOVOZYMES – IN BRIEF
2. OUR PRESENCE IN DISTILLERY
3. LIQUEFACTION PRODUCTS
4. SACCHARIFICATION PRODUCTS
5. PROCESS AIDS & ENHANCER PRODUCTS
6. BENEFITS DERIVED
7. SERVICES OFFERED
Everywhere you need us
Your needs?
More food and
better farming
Cleaner, smarter,
safer production
Economic growth
and job creation
Sustainable cities
and waste recycling
Clean air and
water
Renewable energy A world in
balance
Global consumer
preference
Novozymes is driving the change
to greener fuel and renewable
energy.
Our unique enzymes break
down plants and plant waste
to create ethanol. We increase
plant profitability for both starch-
based and cellulosic ethanol
production and enable new
energy industries.
Plugging into the power of plants
Bioenergy Novozymes
rethinks fuel:
Renewable energy
Less dependence
on fossil fuels
Less CO²
Greater energy security
Conversion of organic
waste
New jobs
Value-chain benefits –creating food, animal
feed and renewable
materials together with
ethanol
Novozymes’ R&D
Novozymes’ R&D innovates
closely with customers and
partners to create superior
results. By investing 14%
of our revenue in R&D,
Novozymes stays on the
cutting edge of innovation.
Our worldwide R&D
community allows us to
develop solutions that meet
both global and local needs.
Novozymes’ R&D: • creates impact for our
customers
• advances industries
• paves the way for a
brighter future
What we do in distillery
Liquefaction Fermentation Distillation Effluent
Treatment
Specialized Products Omnipresent
Specialized Products
Plants are designed to use - and gluco-
amylase but other enzymes may be required due to:
- Feedstock differences - Efficiency improvement - Energy optimization - Yield improvement
Reduced fouling
Viscosity reduction
Running higher solids
Improved fermentation performance
GLUCO-AMYLASE
PROTEASE & PHYTASE
UREA
PROTEASE
ENZYMES AS PROCESSING AIDS
Reduced
fouling
Improved
separation
DRIED DISTILLER GRAINS WITH SOLUBLES
(DDGS)
Improved feed
quality
What the industry wants today….
Hig
h
• Robust process
• Low down time
• Ease of operation
• Efficiency
• Product Quality
Inp
ut
to P
rocess
• Energy (steam)
• Water usage
• Chemical usage
• Effluent generated
• Alcohol generated in fermented mash
• High throughput from existing plant
Lo
w
Low
Output from process
High
Liquefaction
Viscosity
Reduction
01
No jet cooking
03
Optimum
dextrinization
02
Low pH cooking
04
Yield Gain
05
3 + 4 + 5
clubbed
together
06
LIQUEFACTION PRODUCTS
Liquoflow, Liquoflow Basic
• Patented, protein-engineered technology
• Low calcium dependency – doesn’t require add-on’s to improve stability
• Broad pH activity range (pH 5.5 – 6.0)
Liquoflow 4X
• Same technology as Liquoflow
basic & Liquoflow
• More concentrated formulation offers better value
• Ideal choice for large users or overcoming plant limitations
Novozym 25081
• Low pH AA
• Can work as low as 4.7
• Reduces / Eliminates requirement
of alkali in cooking pH adjustments
• Enables higher recycle of thin slop
• Creates safe working environment
Avantec
• Side activity of Gluco-amylase & Protease
• ~2.0% yield gain vs traditional AA’s
• pH profile from 4.8 – 5.8
• Significant energy and chemical savings
• In launch phase in India
High yield Industry Workhorse
Fermentation
Dextrin's to
Glucose
01
Faster
fermentation
process
03
Low residual
starch
02
Yeast nutrition
04
Hydrolyze
difficult starch
05
High yield
06
SACCHARIFICATION PRODUCTS
Saczyme
• Consistent fermentation performance
• Proven track record
Saczyme Plus 2X
• Best-selling GA on the market today
• Optimized component activities for
more complete starch conversion
• Consistently high ethanol yields
Saczyme Pro
• Converts the most difficult starch
fractions
• Most robust performance for
variable mash conditions
• High ethanol yields
Saczyme Yield
• ~1.5% yield gain vs traditional GA’s & 3% energy savings.
• Yield gain from bound starch freed
by cellulase & not from fiber
degradation to Glucose
• In launch phase in India
High yield Industry Workhorse
Access more Starch
0.0%
0.5%
1.0%
1.5%
2.0%
2.5%
3.0%
Yield increase with
Saczyme® Yield
• New enzyme activities release bound
starch from the grain fiber matrix
• 1%-2.5% increases in yield are being
observed in field trials
Industrial scale plant trials with Saczyme Yield
Other glucoamylases hydrolyze
starch on the surface, but cannot
access starch bound in the fiber
matrix.
Saczyme Yield degrades cell walls
to access more starch, allowing for
greater conversion and higher
yields.
Treatment of corn with a traditional
glucoamylase
Saczyme Yield under high magnification Treatment of corn with Saczyme Yield
Traditional glucoamylase under high
magnification
Saczyme Yield degrades fiber to access more starch
VISCOSITY REDUCING ENZYMES (VRE)
A cocktail of enzymes specific for the grain.
Typically includes a formulation of:
• Xylanases
• Cellulases
• Beta-glucanases
Guess which sample has been treated
with Viscosity Reducing Enzymes?
PROTEASE MECHANISM
Proteases for ethanol production have both endo- and exo-activities that contributes to faster amino acid release by cleaving protein in multiple sites
A protease will have a specific amino acid sequence it will recognize and cleave
Reduces complexity of proteins
-
2
ASN
CYS LEU
TYR
ASN
PRO
GLU
GLN
LEU
TYR
ILE
SER
VAL
CYS
SER
THR
CYS
CYS
GLN
GLU
ILE
GLY
PHE
GLN
ARG
LYS
GLN
PRO
PRO
GLY
GLU LEU
ALA LEU
ALA GLN LEU GLY
GLY LEU
GLY
GLY
GLY
LEU
GLU
VAL
ALA
GLY
ALA
LYS
ALA
PRO
ARG
ARG
GLU
ALA
GLN
ASN
ARG
GLY
TYR
THR
TYR
CYS GLY GLU
PHE
LEU VAL
VAL
ALA LEU
GLU
CYS
LEU
GLY
SER
HIS
ASN
GLN
PHE
LEU
VAL
HIS
COOH
NH -
S
S
S
S
S
S
ASN
GLN
VAL
TYR
ALA
LEU
GLU VAL
ALA
GLN
GLY
GLY LEU
GLY
GLY
GLY
LEU
PHE
LEU
Endo-activity
Exo-activity
PROTEASE FOR FOULING REDUCTION
Fouling in the evaporator leaves a
thin black substance that looks
similar to electric tape
Fouling is a large problem for the ethanol industry
Less fouling of equipment - improved downstream efficiency
Other Process Enhancement enzymes
Viscoferm®
• Used with non-rice feedstocks such as wheat, sorghum, cassava
• Reduces viscosity allowing higher DS (dry solids) content
• Improves throughput and lowers energy consumption
Provia®
• Improved yeast nutrition through better amino-acid availability
• Increased conversion efficiency of protein-bound starch
• Reduced chemical costs for nitrogen addition
Olexa ®
• Increased ethanol production due to reduction in glycerol production
• Olexa releases bound oil to significantly increase corn oil yields
Novozymes Customer – together we can…
Reduce fresh water
requirement
Reduce energy
requirement
Reduce effluent
quantity
Improve yields
Reduce Chemical
requirements
Co-product
enhancement
Improved downstream
processes
High Throughput
DELIVER MORE Services fueling success
Novozymes services: Calibrated to meet your plant’s needs
Greatest depth of process knowledge
We want to be your partner and trusted advisor with service offerings geared to give you a competitive edge and help you succeed.
Our services cover a broad range of activities:
• Data analysis and statistics
• Laboratory support
• Process optimization and troubleshooting
• Support for product trials and implementation
• Customer training
• Plant operations support
When science goes big scale
Our purpose