Enteral versus parenteral nutrition: Comparison of energy metabolism in healthy subjects
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0.109 INFLUENCE OF TFIF_PuALITATI~E COMPOSITION OF ENTERAL DIETS ON LIVER AND JEJUNAL MuCOSA. PROTEIN SYNTHESIS. S. Schwartz, M. Farriol, R. Rodriguez, J.B. Padrs, P.E. Vente (Depar- tmentsof Biochemistry , Intensive Care. Ciudad Sanitaria Vall d'Hebron, Barcelona, Spain) Protein synthesis (L- l-Cl4 - Leucine) and concentration of the RNA in the liver and je- junal mucosa were studied. Normal male Sprague-Dowley rats (n = 40) with an initial body weigth of 161.18 + 12.00 g were divided in four groups ( n = 10). Each group received a different commercial formula for human enteral nutrition and water during 8 days. ~11 rats received 50.18 + 4.50 Kcal/d/lOO g BW and 0.335 + 0.042 g N2/d/100 g BW, without statistically significant differences among the groups. Results of protein synthesis: Diet1 Liver Jejunal mucosa [ Absolute mg prot synthesized/ 1 Absolute mg prot synthesized/ NO I synthesis mg RNA I synthesis I mg RNA (mg/d/g BW) (mg/d/g Bw) 1 3.46 + 1.04 18.26 + 6.05 0.39 + 0.05 13.38 + 1.83 2 3.47 + 0.99 21.26 T 5.29 0.34 7 0.09 28.42 + 6.88 3 1.50 7.0.49 4.94 7 0.64 2.37 + 0.19 162.17 + 4.76 4 7.74 T 1.52 39.84 713.27 1.24 + 0.19 79.36 + 2.13 - - - Conclusions: The differences obtained in the protein synthesis and concentration possibility of improving present clinical indications and attaining ter and specific organ and tissue nutrition. of RNA suggest the diets that allow bet-, 0.110 ENTEML VERSUS PARENTEBAL NUTRITION : COMPARISON OF ENERGY METABOLISM IN HEALTHY SUBJECTS. 0. Vernet, L. Christin, Y. Schutz, *E. Danforth, Jr., E. J6quier Institute of Physiology, University of Lausanne, Switzerland, and *The University of Vermont, Metabolic Unit, Burlington, Vt USA. The acute thermogenic, substrates and hormonal responses to nutrients administred either parenterally (IV) or intragastrically (IG) were measured in ten healthy young women (mean weight + SEM : 52 + 1 kg, body fat : 22 t 1 %). After one hour of baseline measurements, a mixture of D-glucose, balanced amino acids (Nutriflexe) and lipid (Intralipide) corresponding to 52 %, 18 % and 30 % of the energy infused respectively was administred for 3 hours at a level equal to 2 times the preinfusion rate of resting energy expenditure (REE) measured by indirect calorimetry. After discontinuation of nutrient administration, the measurements were pursued for a further 3 hours period. Following nutrient administration, mean REE (+ SEM) rose from 0.98 ? 0.02 (IV) and 0.99 + 0.02 (IG) to 1.13 r 0.03 (IV) and 1.13 r 0.02 (IG) resulting in a nutrient induced thermogenesis averaging 10 + 0.7 % (IV) and 9.3 t 0.9 % (IG) of the energy infused. The preinfusion RQ's were 0.81 + 0.02 (IV) and 0.80 f 0.01 (IG), increased to 0.86 r 0.01 (IV) and 0.85 + 0.01 (IG), and following the cessation of nutrient admi- nistration decreased significantly below the preinfusion values : 0.78 i 0.01 (IV) and 0.77 ? 0.01 (IG). The plasma glucose and insulin elevations were significantly higher using the IV route compared to the IG route. It is concluded that although the IG route response time was delayed, both routes of nutrient administration induced comparable thermogenic response and a comparable overall substrates oxidation. However with the IG route this was achieved with lower glycemia and insulinemia. 85
Transcript of Enteral versus parenteral nutrition: Comparison of energy metabolism in healthy subjects
0.109 INFLUENCE OF TFIF_PuALITATI~E COMPOSITION OF ENTERAL DIETS ON LIVER AND JEJUNAL MuCOSA.
PROTEIN SYNTHESIS. S. Schwartz, M. Farriol, R. Rodriguez, J.B. Padrs, P.E. Vente (Depar-
tmentsof Biochemistry , Intensive Care. Ciudad Sanitaria Vall d'Hebron, Barcelona, Spain)
Protein synthesis (L- l-Cl4 - Leucine) and concentration of the RNA in the liver and je- junal mucosa were studied. Normal male Sprague-Dowley rats (n = 40) with an initial body weigth of 161.18 + 12.00 g were divided in four groups ( n = 10). Each group received a different commercial formula for human enteral nutrition and water during 8 days. ~11 rats received 50.18 + 4.50 Kcal/d/lOO g BW and 0.335 + 0.042 g N2/d/100 g BW, without statistically significant differences among the groups.
Results of protein synthesis:
Diet1 Liver Jejunal mucosa
[ Absolute mg prot synthesized/ 1 Absolute mg prot synthesized/
NO I synthesis mg RNA
I synthesis
I mg RNA
(mg/d/g BW) (mg/d/g Bw)
1 3.46 + 1.04 18.26 + 6.05 0.39 + 0.05 13.38 + 1.83 2 3.47 + 0.99 21.26 T 5.29 0.34 7 0.09 28.42 + 6.88 3 1.50 7.0.49 4.94 7 0.64 2.37 + 0.19 162.17 + 4.76 4 7.74 T 1.52 39.84 713.27 1.24 + 0.19 79.36 + 2.13 - - -
Conclusions:
The differences obtained in the protein synthesis and concentration possibility of improving present clinical indications and attaining ter and specific organ and tissue nutrition.
of RNA suggest the diets that allow bet-,
0.110 ENTEML VERSUS PARENTEBAL NUTRITION : COMPARISON OF ENERGY METABOLISM IN HEALTHY SUBJECTS. 0. Vernet, L. Christin, Y. Schutz, *E. Danforth, Jr., E. J6quier Institute of Physiology, University of Lausanne, Switzerland, and *The University of Vermont, Metabolic Unit, Burlington, Vt USA.
The acute thermogenic, substrates and hormonal responses to nutrients administred either parenterally (IV) or intragastrically (IG) were measured in ten healthy young women (mean weight + SEM : 52 + 1 kg, body fat : 22 t 1 %). After one hour of baseline measurements, a mixture of D-glucose, balanced amino acids (Nutriflexe) and lipid (Intralipide) corresponding to 52 %, 18 % and 30 % of the energy infused respectively was administred for 3 hours at a level equal to 2 times the preinfusion rate of resting energy expenditure (REE) measured by indirect calorimetry. After discontinuation of nutrient administration, the measurements were pursued for a further 3 hours period. Following nutrient administration, mean REE (+ SEM) rose from 0.98 ? 0.02 (IV) and 0.99 + 0.02 (IG) to 1.13 r 0.03 (IV) and 1.13 r 0.02 (IG) resulting in a nutrient induced thermogenesis averaging 10 + 0.7 % (IV) and 9.3 t 0.9 % (IG) of the energy infused. The preinfusion RQ's were 0.81 + 0.02 (IV) and 0.80 f 0.01 (IG), increased to 0.86 r 0.01 (IV) and 0.85 + 0.01 (IG), and following the cessation of nutrient admi- nistration decreased significantly below the preinfusion values : 0.78 i 0.01 (IV) and 0.77 ? 0.01 (IG). The plasma glucose and insulin elevations were significantly higher using the IV route compared to the IG route. It is concluded that although the IG route response time was delayed, both routes of nutrient administration induced comparable thermogenic response and a comparable overall substrates oxidation. However with the IG route this was achieved with lower glycemia and insulinemia.
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