Engineering of Biosynthetic Assembly Lines - TU Dortmund Bio-Engineering... · ©Ada Yonath,...
Transcript of Engineering of Biosynthetic Assembly Lines - TU Dortmund Bio-Engineering... · ©Ada Yonath,...
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Engineering of Biosynthetic Assembly Lines
Prof. Dr. Markus Nett
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Structurally diverse
Highly bioactive
Evolutionarily validated
Natural Products as Sources of Medicinal Drugs
Lovastatin Hypolipidemic
Vancomycin Antibiotic
Morphin Analgesic
Paclitaxel Anti-Cancer
Artemisinin Anti-Malaria
Acarbose Anti-Diabetic
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© Ada Yonath, Weizmann Institute
Streptogramin Antibiotics – A Perfect Fit
Pristinamycin IA Pristinamycin IIA
each compounds binds to the 50S subunit of the bacterial subunit
single agent => moderate bacteriostatic effect
combination => potent bactericidal activity 3
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Derivatives Often Make the Better Drugs…
1. (Semi-)Synthetic derivatives
Docetaxel
derived from paclitaxel
improved water solubility
Amoxicillin
derived from benzylpenicillin
extended activity spectrum
oral administration possible
Chloroquine
inspired by quinine
less side-effects
Cheaper production
Simvastatin
inspired by lovastatin
More potent 4
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2. Antibody-drug conjugates (ADCs)
Trastuzumab emtansine
derived from maytansinoids
reduced toxicity
treatment of HEr2-positive
metastatic breast cancer
Derivatives Often Make the Better Drugs…
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3. Unnatural natural products
Derivatives Often Make the Better Drugs…
Precursor-directed
biosynthesis Mutasynthesis
Pathway engineering
Combinatorial biosynthesis
?
Hijacking biosynthetic machinery
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DNA mRNA protein natural product
Informational Flow in Biological Systems
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Blueprints for natural product biosynthesis are genetically fixed
Biosynthetic enzymes select and link the building blocks
Building blocks can be small carboxylic acids, amino acids, sugars,…
Clustering of biosynthesis genes (in bacteria and fungi!)
biosynthesis gene cluster
Principles of Natural Product Biosynthesis
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Assembly-Line Enzymology
Polyketide Synthases (PKS)
Nonribosomal Peptide Synthetases (NRPS)
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genes
proteins
modules
domains
eryAI eryAII eryAIII
DEBSI DEBSII DEBSIII
Organization of Biosynthetic Assembly Lines
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Selection and Loading of PKS Building Blocks
building blocks are acyl compounds
the carboxyl group is activated for capture by nucleophiles
the acyl transfer (AT) domain acts as a gatekeeper:
it selects the correct acyl unit for incorporation
it catalyzes a transthiolation reaction
an acyl carrier protein (ACP) domain holds the thioesterified building block
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PKS Chain Extension
a ketoacyl synthase (KS) domain forms a C-C bond via Claisen condensation
afterwards a chain translocation to the KS domain of the next module occurs
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PKS Reductive Chemistry
ketoreductase (KR) domains reduce the β-keto function
dehydratase (DH) domains eliminate water
enoylreductase (ER) domains generate a fully saturated acyl backbone
in polyketide biosynthesis the reductive steps are optional!
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Assembly of the Macrolide Core in Erythromycin
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Nonribosomal Peptide Synthetases
adenylation (A) select and activate amino acids (or similar substrates)
peptidyl carrier protein (PCP) domains hold the thioesterified building blocks
condensation (C) domains catalyze amide bond formations
T. W
eber
& M
. A. M
ahar
iel,
Stru
ctu
re 2
00
1, 9
, R3
-R9
Bleomycin A2
Ciclosporin
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Example: Biosynthesis of Penicillin G
ACV isopenicillin N (IPN)
6-aminopenicillic acid (6-APA)
phenylacetic acid
penicillin G (benzylpenicillin)
❶ ❷
❸
❸
❶ ACV synthetase
❷ IPN synthase
❸ N-acyl transferase
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Precursor-Directed Biosynthesis (PDB)
Advantages Disadvantages
no genetic manipulation required
broad applicability
simple implementation
empiric method
parental compound is produced
alongside any derivative
=> separation needed
figure taken from: A. Kirschning, F. Taft, T. Knobloch, Org Biomol Chem 2007, 5, 3245-3259
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Proof of Concept: Penicillin V
first made in 1951
feeding of Penicillium chrysogenum with 2-phenoxyethanol
more acid-stable than benzylpenicillin
can be given orally
penicillin V penicillin G
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Generation of Unnatural Myxochelins
Myxochelin A is a potent inhibitor of 5-lipoxygenase:
Schieferdecker, König, Koeberle, Dahse, Werz & Nett, J Nat Prod 2015, 78, 335-338
Korp, König, Schieferdecker, Dahse, König, Werz & Nett, ChemBioChem 2015, 16, 2445-2450
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Mutasynthesis
figure taken from: A. Kirschning, F. Taft, T. Knobloch, Org Biomol Chem 2007, 5, 3245-3259
Advantages Disadvantages
no competition with natural precursors
substrate tolerance can be expanded
more predictable than PDB
producing organism must be
amenable to genetic manipulation
biosynthesis genes must have been
identified
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Proof of Concept: Doramectin
avermectins = anthelmintic macrolides produced by Streptomyces avermitilis
feeding of carboxylic acids to a 2-methylbutyrate-deficient mutant yielded
doramectin, being the first commercial drug made by mutasynthesis
later cyclohexylcarboxyl-CoA biosynthesis genes were introduced from S. collinus
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Limitations of PDB and Mutasynthesis
substrate flexibility of biosynthetic enzymes
PKS discriminate malonyl-CoA and methylmalonyl-CoA
=> extender units in polyketide biosynthesis can hardly be replaced
=> only variation of non-acetate/non-propionate starter units
NRPS are in general a little bit more promiscuous,
but they also tolerate only minor substrate changes, e.g. Ile -> Val
Precursor-directed biosynthesis and mutasynthesis
enable only modest degrees of variation.
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Pathway Engineering
X
selective gene or domain inactivations
X
Advantages Disadvantages
may expose functional groups for
further semisynthetic modifications
reduces the number of compounds
produced
same as mutasynthesis
elimination of biosynthetic functions
often affects yield
only few interventions possible
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0.5 μm
Micacocidin – an Antibiotic Against Mycoplasma spp.
mycoplasmas possess no cell wall
M. peumoniae as leading cause of pneumonia in children
2 million cases/year in the US, including 100,000 hospitalizations
macrolide resistance is on the rise
resistance development under therapy
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A Non-Canonical Assembly
involves a hybrid PKS/NRPS assembly line
features an iteratively acting, partially reducing PKS
M. F. Kreutzer, H. Kage, P. Gebhardt, B. Wackler, H. P. Saluz, D. Hoffmeister, M. Nett, Appl. Environ. Microbiol. 2011, 77, 6117-6124
H. Kage, M. F. Kreutzer, B. Wackler, D. Hoffmeister, M. Nett, Chem. Biol. 2013, 20, 764-771
H. Kage, E. Riva, J. S. Parascandolo, M. F. Kreutzer, M. Tosin, M. Nett, Org. Biomol. Chem. 2015, 13, 11414-11417 25
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Reconstitution and Minimally Inavsive Mutagenesis
Programming of biosynthetic assembly lines
is highly complex! 26
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Combinatorial Biosynthesis
gene cluster A
gene cluster B
Advantages Disadvantages
allows significant structural variation same as mutasynthesis
insufficient understanding of
functional interactions
low success rate
low yields 27
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The Story of Daptomycin
lipopeptide antibiotic from Streptomyces roseosporus
MOA: interference with bacterial cell membrane function
discovered at Eli Lilly in the late 1980s
early clinical trials were discontinued due to muscle toxicity
licensing to Cubist Pharmaceuticals in 1997
altering of the dosing regime minimized muscle toxicity
FDA approval in 2003 for the treatment of skin and skin structure infections
caused by Gram-positive bacteria
R. M. Humphries et al., Clin. Microbiol. Rev. 2013, 26, 759-780
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Daptomycin Biosynthesis
the lipopeptide is the product of a giant assembly line
featuring 13 NRPS modules
a related megasynthetase is found in Streptomyces fradiae
figures taken from: R. H. Baltz, ACS Synth Biol 2014, 3, 748-758 and R. H. Baltz, V. Miao, S. K. Wrigley, Nat Prod Rep 2005, 22, 717-741
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Programming of NRPS – Key Insights
substrate specificity is not only encoded in A domains (!)
C and PCP domains are essential for turnover of intermediates
C-A bidomains have co-evolved as functional units
daptomycin NRPS feature 3 subtypes of C domains: LCL – DCL – Starter-C
PCP domains interact with upstream C and A domains
PCP domains interact with downstream (i) C, (ii) E and C, or (iii) TE domains
figure taken from: K. T. Nguyen et al., Proc Natl Acad Sci USA 2006, 103, 17462-17467
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New Antibiotics from Combinatorial Biosynthesis
table taken from: R. H. Baltz, ACS Synth Biol 2014, 3, 748-758
transplanting of individual A
domains was not successful
bi- and tridomain exchanges
are productive
more than 40 novel
lipopeptide antibiotics
derivatives that are barely
affected by surfactant
reduced mammalian toxicity
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Engineering of biosynthetic assembly lines has evolved from feeding experiments
to precise genetic interventions
These techniques provide access to new drugs
Understanding of the underlying biosynthetic logic is instrumental
Summary
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