En-Te-Hwu (Academia Sinica, Taiwan) - DIY Atomic Force Microscope & Lifelong learning

EDWIN

2015/09/18

DIY ATOMIC FORCE MICROSCOPE(AFM)

&

LIFELONG LEARNING

Outline

• Different microscopes for different scales

• Hands-on learning: DIY AFM

• Microscope for children

• Lifelong learning: LEGO2NANO summer school

• Air pollution (PM 2.5) particles detection

• Online science participation: Crowdcrafting

Exploring the micro world by an optical microscope

Seeing is believing?

Diffraction limit of light

100~20k USD We can not “see” the nanoworld

Scanning Electron Microscope (SEM)

http://www.jeolusa.com/PRODUCTS/ScanningElectronMi

croscopes(SEM)/SEM-FIB/JIB-

4600FMultiBeam/tabid/543/Default.aspx

500k~2M USD

Image only conductive samples

Electron gun

DetectorMagnetic lens

Electron

“touching” the nanofeatures ….

Image both conductive and non-conductive samples in

air, liquid and vacuum environments

50k~500k USD https://en.wikipedia.org/wiki/Atomic_force_microscopy

Atomic force microscope (AFM)

Exploring the Nano world By a very sharp tip….

Year 2013, Francois was looking for

an affordable AFM that can be easily built

10

Nano optics

replaced by DVD OPU Head Nano scanner

replaced by piezo buzzers

Metal parts replaced

by structures made of PCBs Optical table replaced

by handheld anti-vibration table

4 techniques make the AFM affordable

Inspired by Sebastian Butefisch

Outline







13

The DIY AFM kit

http://www.stromlinet-nano.com/






Primary / junior high school children

assembling / using the DIY AFM

Tearing the parts AFM base Buzzer scanner

AFM head Within 2 hours Measuring nanostructures

Lego2Nano Lego2Nano Lego2Nano

15

Move the AFM

from Clean Room to Class Room

Outline







Microscope for children

in the past 100 years: 1,000x

Onion cells

Area: 200 x 200 mm

Optical microscope for children

Microscope for children

Now: Magnification 100,000,000 x

DNA origami on Mica substrate

Area: 5 x 5 mm, AC mode

AFM for children

DNA origami

Sample prepared by Bryan Wei @Tsinghua University http://www.stromlinet-nano.com/


AFM topographic image

Area: 5x5 mm

Optical image

Area: 600x600 mm

Measuring surface of an eraser


Area: 15x15 mm

Optical image

Area: 600x600 mm

Measuring fresh leaf surface


Area: 8x8 mm

Optical image

Area:900x900 mm

Measuring data tracks on a

DVD disk

22

AFM Measurement

3D printer

Nano topography data 3D “Solid data”

New idea!

Science project demonstration

AFM data & 3D printing

DIY AFM Laser microscope mode

Imaging astrocytes

1000X OM phase contract image

with Oil lens (Contrast: 0.143) Laser microscope mode (Contrast: 0.224)

10 mm 10 mm

Wesley W. HSIAO,Hsien-Shun LIAO,Hsing-Hung LIN, Yueh-Lun LEE, Chia-Kwung FAN, Chien-Wei LIAO, Po-Yen LIN, En-Te HWU,

and Chia-Seng CHANG , Analytical Sciences, 29(9), 885 (2013)

Outline







LEGO2NANO Summer school

14~28th August 2015

Reinvent the DIY AFM

Building system design

(MIT Media lab)

http://buildinprogress.media.mit.edu/projects/2667/steps

Outline







http://aqicn.org/city/beijing/

Unhealthy…..

http://aqicn.org/city/beijing/

Air pollution (PM 2.5) particles found

on the DVD data tracks in Beijing

Diameter ~2,300 nm (PM2.5)

Outline







Crowdcrafting and maybe EpiCollect

BY Daniel Lopez Martinez @ LEGO2NANO 2015 SUMMER SCHOOL

Thank you !!

En-Te-Hwu (Academia Sinica, Taiwan) - DIY Atomic Force Microscope & Lifelong learning

Education

Transcript of En-Te-Hwu (Academia Sinica, Taiwan) - DIY Atomic Force Microscope & Lifelong learning