Electronic Supplementary Information (ESI†) · aChemistry Division, Bhabha Atomic Research...
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Electronic Supplementary Information (ESI†) Covalent bridging of Surface Functionalized Fe 3 O 4 and YPO 4 : Eu Nanostructures for Simultaneous Imaging and Therapy K. C. Barick a,* , Anusha Sharma a , Neena G. Shetake b , R. S. Ningthoujam a , R. K. Vatsa a , P. D. Babu c , B. N. Pandey b , P. A. Hassan a,* a Chemistry Division, Bhabha Atomic Research Centre, Mumbai – 400085, India b Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Mumbai – 400085, India c UGC-DAE Consortium for Scientific Research, Mumbai – 400085, India * Corresponding authors: E-mail: [email protected] (K. C. Barick), [email protected] (P. A. Hassan); Fax: + 91 22 2550 5151; Tel: + 91 22 2559 0284 Cell culture procedure: Human lung carcinoma (A549) was obtained from National Centre for Cell Sciences, Pune, India. Cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM: GIBCO, Invitrogen, Carlbad, CA, USA) supplemented with 10% fetal calf serum (FCS: Himedia Laboratories, Mumbai, India) and antibiotics (100 U ml-1 penicillin and 100 μg ml-1 streptomycin) in a humidified atmosphere of 5% CO 2 at 37 °C. The desired number of cells were seeded in complete DMEM and incubated at culture conditions for overnight, followed by treatment with MLHN. After exposure of AC magnetic field to the controls and MLHN treated cells, cells were cultured for 48 h. Then, the media containing MLHN was carefully removed and the cells were further incubated with 0.5 ml of MTT solution (0.5mg/ml, Sigma, USA) at culture conditions for 2 h. The supernatant was aspirated and 1 ml of DMSO was added to each culture dish to solubilize the MTT crystals. The crystals were thoroughly dissolved and further diluted (1: 10) with dimethyl sulfoxide (DMSO). 200 μl of above solution from P-30 culture dishes was transferred to 96 well plates and the blue colour was read in a microplate reader (Tecan infinite 200 PRO, Switzerland) at 544 nm. The cell viability was calculated by comparing the absorption of treated cells to that of control, which was defined as 100%. Electronic Supplementary Material (ESI) for Dalton Transactions. This journal is © The Royal Society of Chemistry 2015
Transcript of Electronic Supplementary Information (ESI†) · aChemistry Division, Bhabha Atomic Research...
Electronic Supplementary Information (ESI†)
Covalent bridging of Surface Functionalized Fe3O4 and YPO4: Eu Nanostructures for Simultaneous Imaging and Therapy
K. C. Baricka,*, Anusha Sharmaa, Neena G. Shetakeb, R. S. Ningthoujama,R. K. Vatsaa, P. D. Babuc, B. N. Pandeyb, P. A. Hassana,*
aChemistry Division, Bhabha Atomic Research Centre, Mumbai – 400085, IndiabRadiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Mumbai – 400085, India
cUGC-DAE Consortium for Scientific Research, Mumbai – 400085, India
*Corresponding authors: E-mail: [email protected] (K. C. Barick),
[email protected] (P. A. Hassan); Fax: + 91 22 2550 5151; Tel: + 91 22 2559 0284
Cell culture procedure:
Human lung carcinoma (A549) was obtained from National Centre for Cell Sciences, Pune, India.
Cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM: GIBCO, Invitrogen, Carlbad,
CA, USA) supplemented with 10% fetal calf serum (FCS: Himedia Laboratories, Mumbai, India) and
antibiotics (100 U ml-1 penicillin and 100 μg ml-1 streptomycin) in a humidified atmosphere of 5%
CO2 at 37 °C. The desired number of cells were seeded in complete DMEM and incubated at culture
conditions for overnight, followed by treatment with MLHN.
After exposure of AC magnetic field to the controls and MLHN treated cells, cells were cultured
for 48 h. Then, the media containing MLHN was carefully removed and the cells were further
incubated with 0.5 ml of MTT solution (0.5mg/ml, Sigma, USA) at culture conditions for 2 h. The
supernatant was aspirated and 1 ml of DMSO was added to each culture dish to solubilize the MTT
crystals. The crystals were thoroughly dissolved and further diluted (1: 10) with dimethyl sulfoxide
(DMSO). 200 μl of above solution from P-30 culture dishes was transferred to 96 well plates and the
blue colour was read in a microplate reader (Tecan infinite 200 PRO, Switzerland) at 544 nm. The
cell viability was calculated by comparing the absorption of treated cells to that of control, which was
defined as 100%.
Electronic Supplementary Material (ESI) for Dalton Transactions.This journal is © The Royal Society of Chemistry 2015
Induction coil
Sample suspension in
eppendorf
Optical fiber for monitoring temperature
Eppendorf
Thermocol
Fig. S1. Photograph of induction coil showing arrangements for time-dependent calorimetric
measurements.
Fig. S2. TEM micrographs of (a) AFLN and (b) MLHN.
Fig. S3. Normalized absorbance vs. time plot of MLHN in aqueous and cell culture mediums.
(a) (b)
Fig. S4. Field dependence of magnetization (M vs. H) plots of hybrid nanostructures (prepared with
6:1 weight ratio of AFLN and CFMN) at 5 and 300 K (inset: expanded M vs. H plot of MLHN at low
field region). The hybrid nanostructures with 6:1 weight ratio of AFLN and CFMN were prepared
using 5 mg CFMN, 30 mg AFLN, 0.33 ml EDC and 0.67 ml NHS. The maximum magnetizations of
hybrid nanostructures prepared with 6:1 weight ratio of AFLN and CFMN is slightly lower than that
of hybrid nanostructures prepared with 4:1 weight ratio of AFLN and CFMN. This is mainly due to
the presence of higher amount of non-magnetic AFLN in the hybrid sample.
Fig. S5. Emission spectra of MLHN prepared with 6:1 weight ratios of AFLN and CFMN recorded at
excitation wavelength of 260 nm using 375 nm filter. The luminescent intensity of MLHN prepared
with 6:1 weight ratios of AFLN and CFMN is higher than that of MLHN prepared with 4:1 weight
ratios of AFLN and CFMN. This enhancement in luminescent intensity can be attributed to the
presence of larger amount of AFLN in the hybrid sample.
550 600 650 700 7500
5
10
15
20
Wavelength, (nm)
Eu3+
Eu3+
Eu3+
f-f
Slit Width = 10 nm
Slit width = 5 nm
exc = 364 nm
Inte
nsity
(a. u
.) X
102
550 600 650 700 750
0
20
40
60
Slit Width = 5 nm
exc = 364 nm
Eu3+
f-fEu
3+
Eu3+
Slit Width = 10 nmInte
nsity
(a. u
.) x
103
Wavelength, (nm)
Fig. S6. Emission spectra of (a) AFLN and (b) MLHN recorded at excitation wavelength of 364 nm
with different slit width of excitation and emission windows.
200 250 300 350 400 450
0
5
10
15
20
25
30
35
Slit Width = 10 nm
Slit width = 5 nm
Eu3+
Eu3+
Eu3+
Eu3+
f-fEu
-O C
TB
em = 612 nm
Inte
nsity
(a. u
.) x
103
Wavelength, (nm) 200 250 300 350 400 450
0.0
0.5
1.0
1.5
2.0
2.5
3.0
Wavelength, (nm)
Slit Width = 10 nm
Slit width = 5 nm
em = 612 nm
Eu3+Eu
3+ Eu3+
Eu3+
f-f
Eu-O CTB
Inte
nsity
(a. u
.) x
103
Fig. S7. Excitation spectra of (a) AFLN and (b) MLHN by monitoring wavelength at 612 nm with
different slit width of excitation and emission windows.
(a) (b)
(a) (b)
Fig. S8. Fluorescence microscopy image of MLHN powder mounted on glass slide.
Table S1. Zeta-potential of MLHN incubated with BSA for different time.
Sample Zeta-potential of sample
in 0.01M PBS (pH 7.3)
Zeta-potential of sample incubated with BSA(0.025
mg/ml) in 1 ml of 0.01 PBS (pH 7.3)
MLHN -26.7 -27.5 -26.7 -27.5 -26.4
