ACTIVITIES OF THE GROUP OF HIGH PERFORMANCE INTEGRATED CIRCUITS DESIGN GROUP

Fast Electrical Impedance Spectroscopy measurement techniques for dynamicbioimpedance characterization.

Ph.D. Student: B.SnchezThesis Advisor: R.Brags
Electronic Engineering DepartmentUniversitat Politcnica de Catalunya

Contents
1.- Introduction2.- Motivation3.- Multitone Burst Signal4.- Results5.- Conclusions

Laplace/Fourier Transform
Transfer Function
Linear Time Invariant system: Impedance

Introduction
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1st Barcelona Forum on Ph.D. Research in Electronic Engineering *
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Extracellular matrix
Cell membrane
HF
LF
Biological systems: Bioimpedance

Introduction
Equivalent Circuit

Ex-vivo tissue characterization:

Introduction
3

Introduction
In-vitro static cell culture growth characterization:
4

Motivation
Challenge (I): In-vitro myocites cell culture growing characterization for tissue engineering applications
5

Link video

Challenge (II): In-vivo myocites patch monitoring and characterization:

Motivation
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Multitone Burst Signals
Time domain:
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Multitone Burst Signals
Frequency domain:
8

Newman:
Schroeder:

Genetic Algorithm:
Multitone Burst Signals
9
Crest Factor:

Frequency Plan:
Avoid intermodulation products: shift original tones for BW guard.

Multitone Burst Signals
10

Multitone bursts with Bilateral Quasi Logarithmic Frequency Distribution:

Multitone Burst Signals
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Measurement Hardware Setup:

Results
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Custom FPGA based arbitrary signal generator.DAC 12bits, 16Msamples, 40Ms/s, 1st order hold.Ethernet capable controller.
PXI solutionPXI-5422: AWG, 200Ms/s, 32MB,16 bits. PXIe-5122: AD 2channels, 100Ms/s, 64MB/channel, 14bits.PXI-2530: multiplexation matrix, 128 channels.

Estimation of the Growth Cell Curve

Results
Yeast cell suspension settlement characterization:
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Conclusions
Non invasive and non destructive technique for passive electrical properties charaterization of biological materials.

Valid measurement method for cell growth estimator and suitable for dynamic biological materials and systems characterization.

Wide application areas: electroporation, tomography, cell counting in microfludic, biosensors, tissue engineering,..

The final application field is the cardiac tissue characterization in regenerative medicine applications.
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Fast Electrical Impedance Spectroscopy measurement techniques for dynamicbioimpedance characterization.

Ph.D. Student: B.SnchezThesis Advisor: R.Brags
Electronic Engineering DepartmentUniversitat Politcnica de Catalunya

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