Effectiveness of Lysol with Hydrogen Peroxide as Disinfectant

Against Escherichia coli

INTRODUCTION

Disinfectants are used extensively in hospitals and other health care settings for a variety of topical and hard-surface applications. They are an essential part of infection control practices.

REVIEW OF RELATED LITERATURE

Lysol is commonly used as disinfectant in the laboratory. One of its product harnesses the versatility of hydrogen peroxide to deliver a more powerful clean than bleach. Lysol with hydrogen peroxide release thousands of micro-bubbles that penetrate deep to remove tough stains, leaving behind a fresh, clean scent with no chemical residue. (Parsippany, 2015)

INGREDIENT FUNCTIONWater DiluentDipropylene Glycol Butyl Ether SolventHydrogen Peroxide Antimicrobial Active

Citric Acid Complexing/Sequestering Agent

Sodium Methyl 2-Sulfolaurate Anionic SurfactantC10 Ethoxylated Alcohol Nonionic SurfactantFragrance/Parfum Fragrance

REVIEW OF RELATED LITERATURE

Ingredient Information

INGREDIENT FUNCTIONTetrasodium Iminodisuccinate

Complexing/Sequestering Agent

Capryl Glucoside Nonionic SurfactantHexyl Cinnamal Fragrance ComponentAlkyl glycoside C10-16 Nonionic SurfactantLinalool Fragrance Component

REVIEW OF RELATED LITERATURE

(Reckitt Benckiser Inc.)

Hydrogen Peroxide

The literature contains several accounts of the properties, germicidal effectiveness, and potential uses for stabilized hydrogen peroxide in the health-care setting. Published reports ascribe good germicidal activity to hydrogen peroxide and attest to its bactericidal, virucidal, sporicidal, and fungicidal properties.

Hydrogen peroxide works by producing destructive hydroxyl free radicals that can attack membrane lipids, DNA, and other essential cell components. Catalase, produced by aerobic organisms and facultative anaerobes that possess cytochrome systems, can protect cells from metabolically produced hydrogen peroxide by degrading hydrogen peroxide to water and oxygen. This defense is overwhelmed by the concentrations used for disinfection. (CDC, 2008)

REVIEW OF RELATED LITERATURE

Minimum inhibitory concentrations (MICs) are defined as the lowest concentration of antimicrobial that will inhibit the visible growth of a micro-organism after overnight incubation, and minimum bactericidal concentrations (MBCs) the lowest concentration of antimicrobial that will prevent the growth of an organism after sub-culture on to antibiotic-free media. MICs are used by diagnostic laboratories, mainly to confirm resistance, but most often as a research tool to determine the in-vitro activity of new antimicrobials. MBC determinations are undertaken less frequently and their major use has been reserved for isolates from the blood of patients with endocarditis. (Andrews, 2001)

STATEMENT OF THE PROBLEM

The study will determine if lysol with hydrogen peroxide is effective as disinfectant against the bacteria, Escherichia coli.

Specifically, the following questions are needed to be answered:

1. What is the minimum inhibitory concentration (MIC) of lysol with hydrogen peroxide?

2. What is the minimum bactericidal concentration (MBC) of lysol with hydrogen peroxide?

HYPOTHESISAlternative Hypothesis

• Lysol with hydrogen peroxide is effective as disinfectant against Escherichia coli.

Null Hypothesis• Lysol with hydrogen peroxide is not effective as

disinfectant against Escherichia coli.

Methodology

Preparation

Serial Dilution

Adding of Bacterial Broth

Incubation

Methodology

INCUBATION

Results and Discussion

TUBE CONCENTRATION APPEARANCE INTERPRETATION1 1:1 Clear No Growth

2 1:2 Clear No Growth

3 1:4 Clear No Growth

4 1:8 Clear No Growth

5 1:16 Turbid With Growth

6 1:32 Turbid With Growth

Minimum Inhibitory Concentration of Lysol with Hydrogen Peroxide

Results and Discussion

The table showed the results of the test for the minimum inhibitory concentration of lysol. Tube 1 containing 1:1 concentration of lysol had a clear solution showing no bacterial growth. Tube 2, 3 and 4 containing 1:2, 1:4 and 1:8 concentration of lysol respectively, showed the same result as tube 1. While, tube 5 and 6 with the concentration of 1:16 and 1:32 respectively, had a turbid solution showing bacterial growth.

ConclusionBased on the results of the experiment, the

students conclude that lysol with hydrogen peroxide is very effective as disinfectant and the minimum inhibitory concentration is 1:8 concentration.

ReferencesAndrews, J.M. (2001). The British Society for Antimicrobial Chemotherapy. Retrieved at http://jac.oxfordjournals.org/content/48/suppl_1/5.abstract on November 25, 2016 Brown , F., Dowlut S. and Karnik T. (2013). Disinfectant Efficacy Testin. Retrieved at http://www.kvh.org.nz/vdb/document/91553 on November 25, 2016 Jozala, A.F., Mazzola, P.G., and Moriel, P. (2009). Brazilian Journal of Pharmaceutical Sciences vol. 45. Retrieved at http://www.revistas.usp.br/bjps/article/viewFile/10677/12445 on November 25, 2016

ReferencesChand, S., Kumar, P., and Saha, K. (2016). International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 5. Retrieved at http://www.ijcmas.com/vol-5-1/Subhash%20Chand,%20et%20al.pdf on November 25, 2016 Andrews, J.M. (2006). Determination of Minimum Inhibitory Concentrations. Retrieved at http://bsac.org.uk/wp-content/uploads/2012/02/Chapter-2-Determination-of-MICs-2006updated.pdf on November 25, 2016 Reckitt Benckiser Inc. http://rbnainfo.com

ReferencesCDC (2008). Guideline for Disinfection and Sterilization in Healthcare Facilities. Retrieved at https://www.cdc.gov/hicpac/Disinfection_Sterilization/7_0formaldehyde.html on November 25, 2016 Parsippany, N.J. (2015). Lysol Launches Line of Hydrogen Peroxide Products That Marks a New Era in Household Cleaning. Retrieved at http://search.ebscohost.com/ on November 25, 2016