Effect of UV Rays on the Colonial & Cellular Morphology and Catalase Activity of Baker’s and Wild...
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Transcript of Effect of UV Rays on the Colonial & Cellular Morphology and Catalase Activity of Baker’s and Wild...
Effect of UV Rays on the Colonial & Cellular Morphology and
Catalase Activity of Baker’s and Wild Strains of Yeast
Amna Jalil
Roll No: 17
Objectives Effect of UV on the colonial morphology
of wild as well as baker’s yeast
Effect of UV on the cellular morphologyof wild as well as baker’s yeast
Effect of UV on the catalase activity ofwild as well as baker’s yeast
Introduction UV light -- electromagnetic radiation
Wavelength shorter -- visible light
longer -- X-rays
400 nm and 10 nm.
Does not break the DNA chain outright
Selectively absorbed by the aromatic
rings of the purine and pyrimidine
bases
Formation of pyrimidine dimers
Two adjacent pyrimidines in the same
chain (T-T, C-C, or T-C) become
covalently bonded together
These dimers disrupt the local
structure of the DNA double helix and
prevent normal DNA replication
Materials and MethodsCollection of the Yeast Sample1. Soil sample
2. Baker’s Yeast
Spreading on YPD Agar platesIsolation of Pure StrainsExposure of UV RadiationsAnalyzing the Effect of UV on Colony
Morphology and Cellular MorphologyAnalyzing the effect of UV on Catalase Activity
Results & Discussion Effect of UV on the colonial morphology of
Baker’s Yeast Sample
Property Before UV ExposureAfter UV Exposure
(1 min)
1. Form Circular Circular
2. Elevation Convex Raised
3. Margins Entire Entire
4. Color Cream colored Cream colored
5. Surface Smooth & Shiny Rough & Matt
Colony morphology of Baker’s yeast
before & after UV exposure
Before UV Exposure After UV Exposure
Effect of UV on the colonial morphology of wild Yeast Sample
Property Before UV ExposureAfter UV Exposure
(1 min)
1. Form Circular Circular
2. Elevation Convex Raised
3. Margins Entire Entire
4. Color Cream colored Cream colored
5. Surface Smooth & Shiny Rough & Matt
Colony morphology of wild yeast before
& after UV exposure
Berore UV Exposure After UV Exposure
Effect of UV on the Cellular
Morphology of Yeast Sample
The lethal effects of the UV radiation
Mutations of the genes or
chromosomes
Destruction of the cell-membrane
Some structures or cell organelles
Cellular morphology of Baker’s yeast
before & after UV exposure
Before UV Exposure After UV Exposure
Cellular morphology of wild yeast before
& after UV exposure
Before UV Exposure After UV Exposure
Effect of UV on the Catalase
Activity of Yeast Sample
UV irradiation on the catalase activity
was divisible into 4 periods.
1. First, the period during which the
cells lost their ability to form colonies,
but during which no change in
catalase activity was noted.
2. Second, the period during which a
considerable rise in catalase activity
occurred.
1. Third, a rather long period during
which irradiation led to no diminution
in the catalase activity of the
maximally active suspension.
2. Fourth, the period of
photoinactivation of the intracellular
enzyme, which was quite similar to
that of the crystalline enzyme in vitro.
Catalase activity of Baker’s yeast before
& after UV exposure
Before UV Exposure After UV Exposure
Catalase activity of wild yeast before &
after UV exposure
Before UV Exposure After UV Exposure