Effect of Substrate Conecentration

7/25/2019 Effect of Substrate Conecentration

1/13

EFFECT OF THE

SUBSTRATE

CONCENTRATION


2/13

OBJECTIVE: Determine how the concentration of the

substrate affects the rate of the enzymatic

activity


3/13

PROCEDURE: Prepare 4 clean and dry test tubes and label

them from 1 to 4. Follow the table as to the

contents of the tube.

Contents Tube 1 Tube 2 Tube 3 Tube 4

1% starchsolution

1.0 mL .0 mL !.0 mL ".0 mL

Distilledwater

1.0 mL 1.0 mL 1.0 mL 1.0 mL

#aliva 1.0 mL 1.0 mL 1.0 mL 1.0 mL


4/13

PROCEDURE: $LL &'(# ) *(+(,-( ( #$L,-$ $

$PP*/,)$(L ( #$)( ,)(. horou2hly

mi3 all the tubes and set aside for " minutes

in a water bath set at !5+. *emove all the tubes from the water bath at

the same time.

Prepare another set of 4 tubes6 labeled as 1a

to 4a. $dd .0 mL of 'enedict7s solution intoeach of the tubes.


5/13

PROCEDURE: $dd " drops from your incubated mi3ture6 i.e.6

tube 1 to tube 1a6 tube to tube a6 and so on.

)i3 thorou2hly.

8arm all the tubes in a boilin2 water bath for

minutes. 9ote the chan2es in the color of the

solution and the resultin2 precipitation:if any;.

*an< the tubes accordin2ly:1=2reatest di2estion

and !=least or no di2estion;. *ecord all your

observations. $ bric


6/13

RESULTS

TEST TUBE OBSERVATIONS

1:1.0 mL starch; 'lue=2reen color

:.0 mL starch; >reen color

!:!.0 mL starch; >reen with a hint of oran2e

4:".0 mL starch; ellow 2reen


7/13

UESTIONS

$. ,n this procedure6 identify the enzyme6 the

substrate6 and the manipulated variable.

(nzyme= #aliva :amylase;

#ubstrate= #tarch solution6 which is themanipulated variable.


8/13

'. ow would you compare the de2ree of

di2estion in the different amounts of starch

solution added?

$s the amount of starch solution:substrate;

increases6 the de2ree of di2estion also

increases.

his is because more substrate molecules will be

collidin2 with enzyme molecules6 so more

product will be formed.

owever6 after a certain concentration6 anyincrease will have no effect on the rate of

reaction.


9/13

+. Provide an e3planation for your observations in

the different amounts of starch solution.

he rate of reaction is dependent on the

concentration of the substrate added.

he hi2her the substrate concentration6 the

more chan2e in color.


10/13

D. Draw a 2raph showin2 the relationship

between enzyme activity and the amount of

substrate.


11/13

(. ow can 'enedict7s rea2ent determine the de2ree

of di2estion in the 2iven tubes? (3plain the principle of

the test and 2ive the reaction.

Bene!"#t$s so%ut"on "s use! &o' !ete#t"n( t)e*'esen#e o& +%!e),!es +n! e+s"%, o-"!".+b%e su(+'s "s

s+/*%es0 )en t)e test s *os"t"e t)e )e+te!

so%ut"on #)+n(es #o%ou' &'o/ b%ue to ('een ,e%%o

o' b'on !e*en!"n( on t)e #on#ent'+t"on o& t)ee+s"%, o-"!".+b%e subst+n#es *'esent0

Re+#t"ons:

*educin2 su2ars are o3idized by the copper ion in

solution to form a carbo3ylic acid and a reddishprecipitate of copper :,; o3ide.


12/13

F. ,n which tube did you observe the 2reatest

di2estion? Least di2estion? Provide an e3planation

for your observations.

he tube with the 2reatest di2estion is test

tubes ! and 4.

he tube with least di2estion is test tube 1.


13/13

>. 8hat is the health implication of this

procedure?

5et+bo%"s/ = +han2in2 the (nzyme and

#ubstrate concentrations affect the rate of

reaction of an enzyme=catalysed reaction.

Fee' = enzymes are dependent on

temperature.

*H= havin2 a balanced p in our body is a

Effect of Substrate Conecentration

Documents

Transcript of Effect of Substrate Conecentration