Effect of laser, ultrasound and irrigation solutions on bond-strength of fiberglass-posts
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e86 dental materials 29S (2013) e1–e96 175 MMPs activity in dentin collagen: Static and dynamic investigation G. Turco 1,∗ , A. Frassetto 1 , A. Mazzoni 1 , M. Cadenaro 1 , D.H. Pashley 2 , L. Breschi 1 1 University of Trieste, Trieste, Italy 2 Georgia Regents University, Augusta, GA, USA Purpose: Despite numerous studies suggested that host- derived matrix metalloproteinases (MMPs) can degrade the dentin collagen network, no information is available on the role of occlusal forces on MMPs enzymatic activity. The aim of this study was to determine the effect of chewing simu- lation (CS) on MMPs activity quantifying the release of ICTP collagen fragments. The tested hypothesis was that CS had no influence on the dentin MMPs activity. Methods and materials: Dentin slabs 1.0 ± 0.1 mm-thick were obtained from human sound teeth and completely dem- ineralized in 10 wt% phosphoric acid, (pH = 1) for 24 h at 25 ◦ C. After demineralization the demineralized dentin slabs were cut in circular disks of 6.0 ± 0.2 mm in diameter using a surgi- cal biopsy punch. Demineralized specimens were then equally and randomly assigned to the following aging treatments: CS (37 ◦ C in 0.5 mL artificial saliva, submitted to 50 N occlusal load, 30 s occlusal time plus 30 s with no load, 1 Hz up to 30 days) or static (control; 37 ◦ C in 0.5 mL artificial saliva for the same time intervals). Total MMP activity was assayed by quantifying ICTP collagen fragments using an ELISA kit at 1, 2, 3, 4, 5, 6, 7, 10, 14, 21 and 30 days. Statistical differences between the data were investigated using t-test for independent data. Results: Results are shown in Fig. 1: ICTP release as func- tion of the tested evaluating points after aging in static or dynamic (CS) conditions. Comparison between the two aging conditions, at the same interval, is significantly different when labeled with an asterisk (*p < 0.05; n.s. = not significant; t-test). Conclusion: The tested hypothesis was partially rejected since only at certain time intervals a statistically significant difference in the ICTP release in relation to the aging pro- cedure was observed. Future studies will investigate shorter time intervals to immediately monitor the dentin endoge- nous MMPs activity in when submitted to chewing simulation. Supported, in part, by grants: FIRB RBAP1095CR and PRIN Fig. 1 2009SAN9K5 and 2009FXT3WL from MIUR, Italy and by R01 DE015306 from the NIDCR to DHP (PI). http://dx.doi.org/10.1016/j.dental.2013.08.176 176 Effect of laser, ultrasound and irrigation solutions on bond-strength of fiberglass-posts C.R. Pucci, C.A.T. Carvalho, R.A. Vasconcelos ∗ , A.J.F. Lacerda, M. Gullo, A.C.C. Xavier, S.E.P. Gonc ¸alves, H.M.C. Rêgo Universidade Estadual Julio Mesquita Filho, São Jose Dos Campos, São Paulo, Brazil Purpose: This study evaluated the influence of the appli- cation of Nd: YAG laser, ultrasound and irrigation solutions (saline solution, 2.5% sodium hypochlorite and 2% chlorhexi- dine) on the bond strength of glass fiber posts cemented with resin cement in root canals of bovine teeth. Methods and materials: 99 bovine tooth roots were divided into 3 groups (n = 33) in accordance with the treatment of dentin after the removal procedure: control, laser (Pulse Mas- ter 600 IQ – American Dental Technologies) and ultrasonic (CVDent 1000-CVDentus Brazil). Each group was subdivided into 3 subgroups (n = 11) according to the irrigation solu- tion used during endodontic treatment: saline solution, 2.5% sodium hypochlorite and 2% chlorexidine. After preparation, the fiberglass posts (White Post-FGM) were cemented with adhesive system (Futurabond DC – VOCO) and the resin epoxy cement (Dual Bifix QM-VOCO). Each root was sectioned into 3 slices of 2 mm related to cervical, middle and apical. These slices were subjected to push out test with a speed of 1mm/min. Data were analyzed using two-way ANOVA and Tukey’s test to examine the effects of biomechanical prepa- ration and type of irrigating solution and their interactions on bond strength (alfa 0.05). Results: The results demonstrated that there were differ- ences only in relation to the type of irrigating solution used during the biomechanical preparation (p < 0.01). The results are shown in Table 1. Table 1 – Bond strength in MPA. Irrigation solution Means SD Homogenuos groups NaOCl 5.19 1.72 A Saline solution 6.95 2.13 B Clx 7.35 1.97 B Conclusion: The use of laser or ultrasound did not influence the bond strength of fiber glass cemented with an adhesive system. However, irrigation of root canals with 2.5% sodium hypochlorite reduced the bond strength when compared to teeth irrigated with saline solution or chlorexidine. Chlorex- idine used as an irrigation solution showed the best bond strength results. http://dx.doi.org/10.1016/j.dental.2013.08.177
Transcript of Effect of laser, ultrasound and irrigation solutions on bond-strength of fiberglass-posts
e86 d e n t a l m a t e r i a l s 2 9 S ( 2 0 1 3 ) e1–e96
175
MMPs activity in dentin collagen: Static anddynamic investigation
G. Turco 1,∗, A. Frassetto 1, A. Mazzoni 1, M.Cadenaro 1, D.H. Pashley 2, L. Breschi 1
1 University of Trieste, Trieste, Italy2 Georgia Regents University, Augusta, GA, USA
Purpose: Despite numerous studies suggested that host-derived matrix metalloproteinases (MMPs) can degrade thedentin collagen network, no information is available on therole of occlusal forces on MMPs enzymatic activity. The aimof this study was to determine the effect of chewing simu-lation (CS) on MMPs activity quantifying the release of ICTPcollagen fragments. The tested hypothesis was that CS hadno influence on the dentin MMPs activity.
Methods and materials: Dentin slabs 1.0 ± 0.1 mm-thickwere obtained from human sound teeth and completely dem-ineralized in 10 wt% phosphoric acid, (pH = 1) for 24 h at 25 ◦C.After demineralization the demineralized dentin slabs werecut in circular disks of 6.0 ± 0.2 mm in diameter using a surgi-cal biopsy punch. Demineralized specimens were then equallyand randomly assigned to the following aging treatments: CS(37 ◦C in 0.5 mL artificial saliva, submitted to 50 N occlusal load,30 s occlusal time plus 30 s with no load, 1 Hz up to 30 days)or static (control; 37 ◦C in 0.5 mL artificial saliva for the sametime intervals). Total MMP activity was assayed by quantifyingICTP collagen fragments using an ELISA kit at 1, 2, 3, 4, 5, 6, 7,10, 14, 21 and 30 days. Statistical differences between the datawere investigated using t-test for independent data.
Results: Results are shown in Fig. 1: ICTP release as func-tion of the tested evaluating points after aging in static ordynamic (CS) conditions. Comparison between the two agingconditions, at the same interval, is significantly different whenlabeled with an asterisk (*p < 0.05; n.s. = not significant; t-test).
Conclusion: The tested hypothesis was partially rejectedsince only at certain time intervals a statistically significantdifference in the ICTP release in relation to the aging pro-cedure was observed. Future studies will investigate shortertime intervals to immediately monitor the dentin endoge-nous MMPs activity in when submitted to chewing simulation.Supported, in part, by grants: FIRB RBAP1095CR and PRIN
Fig. 1
2009SAN9K5 and 2009FXT3WL from MIUR, Italy and by R01DE015306 from the NIDCR to DHP (PI).
http://dx.doi.org/10.1016/j.dental.2013.08.176
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Effect of laser, ultrasound and irrigationsolutions on bond-strength offiberglass-posts
C.R. Pucci, C.A.T. Carvalho, R.A. Vasconcelos ∗,A.J.F. Lacerda, M. Gullo, A.C.C. Xavier, S.E.P.Goncalves, H.M.C. Rêgo
Universidade Estadual Julio Mesquita Filho, SãoJose Dos Campos, São Paulo, Brazil
Purpose: This study evaluated the influence of the appli-cation of Nd: YAG laser, ultrasound and irrigation solutions(saline solution, 2.5% sodium hypochlorite and 2% chlorhexi-dine) on the bond strength of glass fiber posts cemented withresin cement in root canals of bovine teeth.
Methods and materials: 99 bovine tooth roots were dividedinto 3 groups (n = 33) in accordance with the treatment ofdentin after the removal procedure: control, laser (Pulse Mas-ter 600 IQ – American Dental Technologies) and ultrasonic(CVDent 1000-CVDentus Brazil). Each group was subdividedinto 3 subgroups (n = 11) according to the irrigation solu-tion used during endodontic treatment: saline solution, 2.5%sodium hypochlorite and 2% chlorexidine. After preparation,the fiberglass posts (White Post-FGM) were cemented withadhesive system (Futurabond DC – VOCO) and the resinepoxy cement (Dual Bifix QM-VOCO). Each root was sectionedinto 3 slices of 2 mm related to cervical, middle and apical.These slices were subjected to push out test with a speed of1 mm/min. Data were analyzed using two-way ANOVA andTukey’s test to examine the effects of biomechanical prepa-ration and type of irrigating solution and their interactions onbond strength (alfa 0.05).
Results: The results demonstrated that there were differ-ences only in relation to the type of irrigating solution usedduring the biomechanical preparation (p < 0.01). The resultsare shown in Table 1.
Table 1 – Bond strength in MPA.
Irrigation solution Means SD Homogenuosgroups
NaOCl 5.19 1.72 ASaline solution 6.95 2.13 BClx 7.35 1.97 B
Conclusion: The use of laser or ultrasound did not influencethe bond strength of fiber glass cemented with an adhesivesystem. However, irrigation of root canals with 2.5% sodiumhypochlorite reduced the bond strength when compared toteeth irrigated with saline solution or chlorexidine. Chlorex-idine used as an irrigation solution showed the best bondstrength results.
http://dx.doi.org/10.1016/j.dental.2013.08.177
