Examination of the Effects of Biosurfactant Concentration ...
Effect of Glucose on Biosurfactant Production using Bacterial Isolates from Oil Contaminated Sites
3
International Journ Internatio ISSN No: 24 @ IJTSRD | Available Online @ www.i Effect of Glucos Bacterial Isol Lec Shri Shivaji ABSTRACT The demand for biosurfactants is gradua and are thus substituting their chemical counterparts [14]. The production of commercially requires high expens production of biosurfactant proper optim physico-chemical parameters is very imp the research was conducted to study glucose as a carbon source for p biosurfactant using bacterial isolate contaminated sites in MSM medium. KEY WORDS: Biosurfactants, Bacte Glucose medium, Minimal Salt Medium. INTRODUCTION Biosurfactants are amhiphatic compou mostly produced by bacterial sp Biosurfactants are reported to be toxic They are biodegradable in nature as com commercially available surfactants [1]. have varied environmental applicati hydrocarbon remediation from soil, dis spills and enhancement of oil recovery 5]. Biosurfactants have potential us industry, agriculture, pharmaceutics, pe paper and pulp industry etc. and therefo the area of biosurfactants has expanded recent years. The development of this li is of great importance, mainly in view concern with protection of the environm nal of Trend in Scientific Research and De onal Open Access Journal | www.ijtsrd.co 456 - 6470 | Volume - 3 | Issue – 1 | Nov ijtsrd.com | Volume – 3 | Issue – 1 | Nov-Dec 20 se on Biosurfactant Production lates from Oil Contaminated S Anjali Sharma cturer, Department of Microbiology, Science College Nagpur, Maharashtra, India ally increasing lly synthesized biosurfactants ses. For the mization of the portant. Hence the effect of production of tes from oil erial isolates, . ounds that are pecies[1,4,17]. c in nature [7]. mpared to other Biosurfactants ions such as spersion of oil y processes [2, se in various etro chemistry, ore research in d quite a lot in ine of research of the present ment. The most significant advantage of a bios surfactants is its ecological ac the other advantages of selectivity, specific activity a pH and salinity and the possib through fermentation [9, 10 biosurfactants have been repo biosurfactant is influenced by enhance the growth of the mic to stimulate biosurfactant pr optimization of ecologica nutritional conditions [15]. MATERIALS AND METHO In order to study effect of glu on production of biosurfactant was used as the basal medium the glucose was kept at 1% culture of isolated bacterial IHD19, IHD21, IHD36, IH IHD89, IHD96, IHD112, IHD IHD 176, IHD178, and IHD18 100 ml of Minimal Salt Medi as carbon source and were inc at 120rpm at 37 ⁰C for 7 day monitored by measuring th Density), A 540 . The biosurf determined by measuring the [2, 12, 13, 16, 18] evelopment (IJTSRD) om v – Dec 2018 018 Page: 730 n using Sites surfactant over chemical cceptance [6,8]. Some of biosurfactants include at extreme temperatures, bility of their production 0]. A large variety of orted [11]. Production of y the factors which also crobe. One strategy used roduction has been the al, physiological and ODS: ucose as a carbon source t, Minimal Salt Medium m. The concentration of % (w/v). 1% inoculum strains (IHD3, IHD13, HD44, IHD58, IHD80, D 148, IHD152, IHD157, 88) were inoculated into ium containing glucose cubated in rotary shaker ys. Growth of cells was he absorbance (Optical factant production was EI% as described under
description
The demand for biosurfactants is gradually increasing and are thus substituting their chemically synthesized counterparts 14 . The production of biosurfactants commercially requires high expenses. For the production of biosurfactant proper optimization of the physico chemical parameters is very important. Hence the research was conducted to study the effect of glucose as a carbon source for production of biosurfactant using bacterial isolates from oil contaminated sites in MSM medium. Anjali Sharma "Effect of Glucose on Biosurfactant Production using Bacterial Isolates from Oil Contaminated Sites" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-1 , December 2018, URL: https://www.ijtsrd.com/papers/ijtsrd19033.pdf Paper URL: http://www.ijtsrd.com/biological-science/microbiology/19033/effect-of-glucose-on-biosurfactant-production-using-bacterial-isolates-from-oil-contaminated-sites/anjali-sharma
Transcript of Effect of Glucose on Biosurfactant Production using Bacterial Isolates from Oil Contaminated Sites
International Journal of Trend in
International Open Access
ISSN No: 2456
@ IJTSRD | Available Online @ www.ijtsrd.com
Effect of Glucose Bacterial Isolates
LecturerShri Shivaji Science College Nagpur
ABSTRACT The demand for biosurfactants is gradually increasing and are thus substituting their chemically synthesized counterparts [14]. The production of biosurfactants commercially requires high expenses. For the production of biosurfactant proper optimization ofphysico-chemical parameters is very important. Hence the research was conducted to study the effect of glucose as a carbon source for production of biosurfactant using bacterial isolates from oil contaminated sites in MSM medium. KEY WORDS: Biosurfactants, BacterGlucose medium, Minimal Salt Medium. INTRODUCTION Biosurfactants are amhiphatic compoundsmostly produced by bacterial speciesBiosurfactants are reported to be toxic in natureThey are biodegradable in nature as compared to other commercially available surfactants [1]. Biosurfactantshave varied environmental applicationshydrocarbon remediation from soil, dispersionspills and enhancement of oil recovery processes5]. Biosurfactants have potential use in industry, agriculture, pharmaceutics, petropaper and pulp industry etc. and therefore research in the area of biosurfactants has expanded quite a lot in recent years. The development of this line of research is of great importance, mainly in view of the present concern with protection of the environment
International Journal of Trend in Scientific Research and Development (IJTSRD)
International Open Access Journal | www.ijtsrd.com
ISSN No: 2456 - 6470 | Volume - 3 | Issue – 1 | Nov
www.ijtsrd.com | Volume – 3 | Issue – 1 | Nov-Dec 2018
f Glucose on Biosurfactant Production Isolates from Oil Contaminated Sites
Anjali Sharma ecturer, Department of Microbiology,
Shri Shivaji Science College Nagpur, Maharashtra, India
The demand for biosurfactants is gradually increasing and are thus substituting their chemically synthesized counterparts [14]. The production of biosurfactants commercially requires high expenses. For the production of biosurfactant proper optimization of the
chemical parameters is very important. Hence the research was conducted to study the effect of glucose as a carbon source for production of biosurfactant using bacterial isolates from oil
Biosurfactants, Bacterial isolates, Glucose medium, Minimal Salt Medium.
hiphatic compounds that are bacterial species[1,4,17].
toxic in nature [7]. biodegradable in nature as compared to other
surfactants [1]. Biosurfactants environmental applications such as
hydrocarbon remediation from soil, dispersion of oil enhancement of oil recovery processes [2,
potential use in various petro chemistry,
therefore research in expanded quite a lot in of this line of research
view of the present concern with protection of the environment. The most
significant advantage of a biosurfactant oversurfactants is its ecological acceptancthe other advantages of biosurfactants includeselectivity, specific activity at extreme temperatures,pH and salinity and the possibility of their productionthrough fermentation [9, 10].biosurfactants have been reportbiosurfactant is influenced by theenhance the growth of the microbe.to stimulate biosurfactant productionoptimization of ecological, physiologicalnutritional conditions [15]. MATERIALS AND METHODS:In order to study effect of glucose as a carbon source on production of biosurfactant, Mwas used as the basal medium.the glucose was kept at 1% (w/v). 1% inoculum culture of isolated bacterial IHD19, IHD21, IHD36, IHD44, IHD58, IHD80, IHD89, IHD96, IHD112, IHD 148, IHD152, IHD157, IHD 176, IHD178, and IHD188) were100 ml of Minimal Salt Medium containing glucose as carbon source and were incubated in rotarat 120rpm at 37 ⁰C for 7 days. Growth of cells was monitored by measuring the absorbance (Optical Density), A540. The biosurfactant production was determined by measuring the EI% as described under[2, 12, 13, 16, 18]
Research and Development (IJTSRD)
www.ijtsrd.com
1 | Nov – Dec 2018
Dec 2018 Page: 730
n Biosurfactant Production using rom Oil Contaminated Sites
significant advantage of a biosurfactant over chemical is its ecological acceptance [6,8]. Some of
the other advantages of biosurfactants include selectivity, specific activity at extreme temperatures, pH and salinity and the possibility of their production through fermentation [9, 10]. A large variety of biosurfactants have been reported [11]. Production of biosurfactant is influenced by the factors which also enhance the growth of the microbe. One strategy used to stimulate biosurfactant production has been the optimization of ecological, physiological and
MATERIALS AND METHODS: glucose as a carbon source
on production of biosurfactant, Minimal Salt Medium as the basal medium. The concentration of
was kept at 1% (w/v). 1% inoculum strains (IHD3, IHD13,
IHD19, IHD21, IHD36, IHD44, IHD58, IHD80, IHD89, IHD96, IHD112, IHD 148, IHD152, IHD157, IHD 176, IHD178, and IHD188) were inoculated into
edium containing glucose were incubated in rotary shaker
C for 7 days. Growth of cells was monitored by measuring the absorbance (Optical
. The biosurfactant production was determined by measuring the EI% as described under
International Journal of Trend in Scientific Research and Development (IJTSRD) ISSN: 2456
@ IJTSRD | Available Online @ www.ijtsrd.com
RESULTS AND DISCUSSION: Table.1. Effect of Glucose as Carbon Sources
pH = 7.00, Temperature: 37 ⁰C. Inoculum = 1 vol%, engine oil = 2 vol%, Incubation time = 7days,
Bacterial
isolates
IHD 3
IHD 13
IHD 19
IHD 21
IHD 36
EI % 64 78 88 69 76 O.D
600 nm 0.7 1.6
1.82
0.8 1.76
A number of carbon sources have been used by many researchers for biosurfactant production. The quality and quantity of produced biosurfactant are affected and influenced by the nature of the carbon substrate. Out of the various carbon sources screened forproduction of biosurfactant in the current study, glucose was found to be the best carbon source for growth and biosurfactant production. The highest Emulsification activity was obtained using glucose at concentration of (1% w/v) as source of carbon alongwith engine oil, resulted in higher emulsifier activity (EI% = 88 % and EA = 1.82). The results were in disagreement with the founding of A. Khopade et.al.,[11] where they obtained maximum activity (EI%= 80%) when using sucrose as source of carbon along with engine oil by marine isolate sp. B3. Maximum growth of bacterial cell and biosurfactant production by bacterial species isolated from petroleum contaminated soil were observed by using glucose as the source of carbon along with engine oil where the maximum value of emulsification index
64
78
88
6976
0
10
20
30
40
50
60
70
80
90
100
International Journal of Trend in Scientific Research and Development (IJTSRD) ISSN: 2456
www.ijtsrd.com | Volume – 3 | Issue – 1 | Nov-Dec 2018
Table.1. Effect of Glucose as Carbon Sources Inoculum = 1 vol%, engine oil = 2 vol%, Incubation time = 7days,
shaking speed = 125 r.p.m. IHD 44
IHD 58
IHD 80
IHD 89
IHD 96
IHD
112
IHD
148
IHD
152 68 75 67 54 66 76 62 73 1.28
1.5 1.07
0.62
0.9 0.6 0.4 0.7
A number of carbon sources have been used by many researchers for biosurfactant production. The quality and quantity of produced biosurfactant are affected and influenced by the nature of the carbon substrate. Out of the various carbon sources screened for production of biosurfactant in the current study, glucose was found to be the best carbon source for growth and biosurfactant production. The highest Emulsification activity was obtained using glucose at concentration of (1% w/v) as source of carbon along with engine oil, resulted in higher emulsifier activity (EI% = 88 % and EA = 1.82). The results were in disagreement with the founding of A. Khopade
where they obtained maximum activity (EI%= 80%) when using sucrose as source of carbon along with engine oil by marine isolate Streptomyces
Maximum growth of bacterial cell and biosurfactant bacterial species isolated from
were observed by using glucose as the source of carbon along with engine oil where the maximum value of emulsification index
was found to be EI%= 88%. which was given by IHD 19, Intermediate range of Emulsification Index were recorded between 79% to 64% given by the bacterial isolates viz.,. IHD 3, IHD 13, IHD 21, IHD 36, IHD 44, IHD 58, IHD 80, IHD 96, IHD 112, IHD 148, IHD 152, IHD 157, IHD 176. IHD 178 and IHD188. Lowest EI% was recorded to be 54% which was given by IHD 89. CONCLUSION: Growth and production medium for biosurfactant production was optimized by using glucose as the carbon source in the Minimal Salt Medium.Maximum production of biosurfactant was observed in MSM medium containing glucose and 2% engine oil. REFERENCES: 1. Ainon, H., Noramiza, S. and Shahidan, R. (2013).
Screening of biosurfactants produced by the hydrocarbon degrading bacteria. Malaysiana, 42 (5): 615 - 623.
6875
67
54
66
76
62
7379
Effect of Glucose
International Journal of Trend in Scientific Research and Development (IJTSRD) ISSN: 2456-6470
Dec 2018 Page: 731
Inoculum = 1 vol%, engine oil = 2 vol%, Incubation time = 7days,
IHD
157
IHD
176
IHD
178
IHD
188 79 77 69 71
0.8 1 0.6 0.9
which was given by IHD 19, Intermediate range of Emulsification Index were
tween 79% to 64% given by the bacterial isolates viz.,. IHD 3, IHD 13, IHD 21, IHD 36, IHD 44, IHD 58, IHD 80, IHD 96, IHD 112, IHD 148, IHD 152, IHD 157, IHD 176. IHD 178 and IHD188. Lowest EI% was recorded to be 54% which was given
Growth and production medium for biosurfactant production was optimized by using glucose as the carbon source in the Minimal Salt Medium. Maximum production of biosurfactant was observed in MSM medium containing glucose and 2% engine
inon, H., Noramiza, S. and Shahidan, R. (2013). Screening of biosurfactants produced by the hydrocarbon degrading bacteria. Sains
623.
7769 71
Effect of Glucose
International Journal of Trend in Scientific Research and Development (IJTSRD) ISSN: 2456
@ IJTSRD | Available Online @ www.ijtsrd.com
2. Anjali Sharma, Dr. A. V. Gomashe, ‘production by bacterial species isolated from petroleum oil contaminated soil’. (2018) IJCRT | Volume 6, Issue 2 April 2018 | ISSN: 2320
3. Anjali Sharma, Dr. A. V. Gomashe“ Isolation and Screening of Biosurfactant Producing Bacterial Species from Petroleum Oil Contaminated Sites”. International Journal of Scientific Progress and Research (IJSPR) ISSN: 2349-4689 Issue 120, Volume 42, Number 03, December (2017).
4. Benincasa, M., Marqués, A., Pinazo, A. and Manresa, A. (2010). Rhamnolipids surfactants: alternatives substrates, new strategies. In. Sen,. R. (Ed.). Biosurfactants, pages. 170 York: Landes Bioscience and Springer Science and Business Media.
5. Banat IM, Franzetti A, Gandolfi I, Bestetti G, Martinotti MG, Fracchia L, Smyth TJ, Marchant R. Microbial biosurfactants producapplications and future potential. Appl Microbiol Biotechnol. 2010;87:427–444.
6. Bailey, R.W. (1958). The reaction of pentoses with anthrone. Biochemical Journal672.
7. Bouchez, M., Blanchet, D. and VandacasteeleP. (1995). Degradation of polycyclic aromatic hydrocarbons by pure strains and defined strain associations: inhibition phenomena and cometabolism. Applied MicrobiologyBiotechnology, 43: 156 - 164.
8. Bordoloi, N. K. and Konwar, B. K. (2009). Bacterial biosurfactant in enhancing solubility and metabolism of petroleum hydrocarbons. of Hazardous Materials, 170: 495 - 505.
9. Donlan R.M. Biofilms: Microbial Life on Surfaces I. Emerging. J. Infectious Diseases. 2002. 8 (3) 881-890.
International Journal of Trend in Scientific Research and Development (IJTSRD) ISSN: 2456
www.ijtsrd.com | Volume – 3 | Issue – 1 | Nov-Dec 2018
Anjali Sharma, Dr. A. V. Gomashe, ‘Biosurfactant production by bacterial species isolated from
(2018) IJCRT | Volume 6, Issue 2 April 2018 | ISSN: 2320-2882.
Anjali Sharma, Dr. A. V. Gomashe, Mayur Rohi, Isolation and Screening of Biosurfactant
Producing Bacterial Species from Petroleum Oil International Journal of
Scientific Progress and Research (IJSPR) ISSN: 4689 Issue 120, Volume 42, Number 03,
Benincasa, M., Marqués, A., Pinazo, A. and Manresa, A. (2010). Rhamnolipids surfactants:
gies. In. Sen,. R. , pages. 170 - 184. New
and Springer Science
Banat IM, Franzetti A, Gandolfi I, Bestetti G, Martinotti MG, Fracchia L, Smyth TJ, Marchant R. Microbial biosurfactants production,
Appl Microbiol
Bailey, R.W. (1958). The reaction of pentoses Biochemical Journal, 68: 669 -
Bouchez, M., Blanchet, D. and Vandacasteele, J. P. (1995). Degradation of polycyclic aromatic hydrocarbons by pure strains and defined strain associations: inhibition phenomena and
Applied Microbiology
Bordoloi, N. K. and Konwar, B. K. (2009). iosurfactant in enhancing solubility and
metabolism of petroleum hydrocarbons. Journal 505.
Biofilms: Microbial Life on Surfaces J. Infectious Diseases. 2002. 8 (3)
10. Hall-Stoodley L., et al. Bacterial Biofilms: From the Natural Environmental to Infectious DiseaseNature Review Microbiology. 2004. 2; 95
11. Khopade, A. N, R. Biao X. Liu,, K Mahadik, L Zhang, K. R. Kokare (2012). Production and stability studies of the biosurfactant isolatmarine B4 species, Elsevier, 198
12. Mullignn, C.N. (2005) Environmental Applications for Biosurfactants. Pollution. 133,183-198
13. Oliveira R, Melo L, Oliveira A, Salgueiro R (1994). Polysaccharide production and biofilm formation by Pseudomonas fluorescenspH and surface material. Colloids Surf. 2:41
14. Soberon-Chavez G, AguirreR(December2005). “The Pseudomonas aeruginosa RhIA enzyme is involved in rhamnolipid and polyhydroxyalkanoate production”.J. InMicrobiol. Biotechol.32 (11
15. Shoeb, E., Akhlaq, F., Badar, U., Akhter, J. and Imtiaz, S. (2013). Classification and industrial applications of biosurfactants. Applied Sciences, 4 (3): 243
16. W. R. Finnerty, “Microbial chydrocarbons products: commercial perspectives,” in Bioconversion of WasteProducts, A. M. Martin, Ed., Elsevier, Amsterdam, The Netherlands, 1991.
17. Xia, W-J., Dong, H-P., Yu, L. and Yu, D(2011). Comparative study of biosurfactant produced by microorganisms isolated from formation water of petroleum reservoir. and Surfaces A: Physicochemical and Engineering Aspects, 392: 124
18. Zhang, Y. and Miller, R. M. (1992). Enhancement of octadecane dispersion and biodegradation by a Pseudomonas rhamnolipid biosurfactant. and Environmental Microbiology3282.
International Journal of Trend in Scientific Research and Development (IJTSRD) ISSN: 2456-6470
Dec 2018 Page: 732
Bacterial Biofilms: From the Natural Environmental to Infectious Disease. Nature Review Microbiology. 2004. 2; 95-108
Khopade, A. N, R. Biao X. Liu,, K Mahadik, L Zhang, K. R. Kokare (2012). Production and stability studies of the biosurfactant isolated from marine B4 species, Elsevier, 198-204.
Mullignn, C.N. (2005) Environmental Applications for Biosurfactants. Environmental
Oliveira R, Melo L, Oliveira A, Salgueiro R (1994). Polysaccharide production and biofilm
Pseudomonas fluorescens: effects of pH and surface material. Colloids Surf. 2:41-46.
Chavez G, Aguirre-Ramirez M, Sanchez R(December2005). “The Pseudomonas aeruginosa RhIA enzyme is involved in rhamnolipid and polyhydroxyalkanoate production”.J. Ind. Microbiol. Biotechol.32 (11-12):675-7.
Shoeb, E., Akhlaq, F., Badar, U., Akhter, J. and Imtiaz, S. (2013). Classification and industrial applications of biosurfactants. Part-I: Natural and
, 4 (3): 243 - 252.
W. R. Finnerty, “Microbial conversion of hydrocarbons products: commercial perspectives,”
Bioconversion of Waste Materials to Industrial , A. M. Martin, Ed., Elsevier,
Amsterdam, The Netherlands, 1991.
P., Yu, L. and Yu, D-F. (2011). Comparative study of biosurfactant produced by microorganisms isolated from formation water of petroleum reservoir. Colloids
Physicochemical and , 392: 124 - 130.
Y. and Miller, R. M. (1992). Enhancement of octadecane dispersion and biodegradation by a
rhamnolipid biosurfactant. Applied and Environmental Microbiology, 58: 3276 -
