EDx Environmental Diagnostics

EDxPrime • EDxPlus

Beyond rack testing.Beyond compare.

Select from two PCR-based testing service options for more customized animal health monitoring with results in just 3 days.

IDEXX BioAnalytics

IDEXX BioAnalytics

Our EDx Environmental Diagnostics offers you the most flexible lab animal health monitoring services available. Regardless of what racks you have, the size of your facility, or how complex your exclusion list is—we’ve got you covered. EDxPrime and EDxPlus are two flexible testing services that allow you to customize your animal health monitoring program for the most effective solution for your facility—and your budget.

More options,more confidence.

A PCR-based animal health monitoring solution to test “just the racks.”

EDxPrime works with most

rack-based sample collection

types including:

• Plenum

• Cage

• Dust

• Filter/Media/Matrices

A customized monitoring solution with expanded sampling options.

EDxPlus includes rack testing

PLUS we’ll partner with you to

design a comprehensive AHM

program to included expanded

sample types such as Opti-Spot®,

feces, and swabs. We’ll help you

determine – from our extensive

assay portfolio and sampling

options – the best monitoring

solution for your facility.

EDxPlusEDxPrime

New EDx Profiles

In addition to these new EDx profiles, select from our full EDx portfolio of assays to customize a testing program that meets your unique health monitoring needs. Or, if you are not sure what service option might work best for you, Dr. Livingston here at IDEXX BioAnalytics and his team can help. They are available to discuss testing options with you and provide insight on the best solution for your facility.

MOUSE Profiles

Fur Mites • EPinworms • ESpironucleus muris • EEntamoeba muris • ETritrichomonas muris • EGiardia spp. • E

Parasite Panel

EDx Parasite Profile

MOUSE Profiles

Fur Mites • EPinworms • ESpironucleus muris • EEntamoeba muris • ETritrichomonas muris • EGiardia spp. • EHelicobacter • EPasteurella pneumotropica • E

EDx Primary* Profile

*Screens for agents not easily transmitted to sentinels

Sample Type*

Primary Panel Sample Type*

*Sample Type:

E = Environmental samples from open air flow design racks

• Specialized matrices or filters designed to capture exhaust air dust

• Flocked swab of rack exhaust air dust (prefilter, plenum)

• A sample of exhaust air dust (200 ul dust)

MOUSE Profiles

Bacteria- RespiratoryPasteurella pneumotropica • • • • E(Jawetz and Heyl biotypes)Myoplasma pulmonis • • • EStaphylococcus aureus • • EStreptococcus, ß-hemolytic • • E(Groups A, B, C, G)Bordetella bronchiseptica • EBordetella hinzii • ECorynebacterium kutscheri • EStreptobacillus moniliformis • EStreptococcus pneumoniae • ECARB • EC. bovis • EBacteria - EntericHelicobacter spp. – H. billis, H. ganmani, H. hepaticus, H. mastomyrinus, H. rodentium, H. typhlonius

• • • • E

Klebsiella oxytoca • • • EKlebsiella pneumoniae • • • ESalmonella spp. • • • ECitrobacter rodentium • • EClostridium piliforme • • EPseudomonas aeruginosa • • ECampylobacter spp. • EProteus mirabillis • EBacteria - SkinCorynebacterium bovis • EStaphylococcus xylosus • EFungiPneumocystis spp. • EVirusesMHV • • • • EMVM • • • • EMPV (1-5) • • • • EMNV • • • • ETMEV • • • • EMRV/EDIM • • • • EMAV1, MAV2 • • EReo Virus 1, 2 and 3 • EPneumonia virus of mice • ESendai virus • EEctromelia • EHantaan virus • ELymphocytic choriomeningitis virus • EParasites Pinworm – Syphacia obvelata, Aspiculuris tetraptera

• • • • E

Fur Mite – Myocoptes, Myobia and Radfordia spp.

• • • • E

Spironucleus muris • • • • EEntamoeba muris • • • • ETritrichomonas muris • • ECryptosporidium spp. • EGiardia spp. • E

Comprehensive GlobalBasicPrevelant

Mouse Standard Profiles

Sample Type*

EDx Profiles are easily customizable for your specific testing needs.

EDx Profiles

MOUSE Profiles

Bacteria- RespiratoryPasteurella pneumotropica • • • • E(Jawetz and Heyl biotypes)Myoplasma pulmonis • • • EStaphylococcus aureus • • EStreptococcus, ß-hemolytic • • E(Groups A, B, C, G)Bordetella bronchiseptica • ECorynebacterium kutscheri • EStreptobacillus moniliformis • EStreptococcus pneumoniae • ECARB • EBacteria - EntericHelicobacter spp. – H. billis, H. ganmani, H. hepaticus, H. mastomyrinus, H. rodentium, H. typhlonius

• • • • E

Klebsiella oxytoca • • • EKlebsiella pneumoniae • • • ESalmonella spp. • • • EClostridium piliforme • • EPseudomonas aeruginosa • • ECampylobacter spp. • EProteus mirabillis • EBacteria - SkinStaphylococcus xylosus • EFungiPneumocystis spp. • EVirusesRCV/SDAV • • • • ERPV • • • • EKRV • • • • EH-1 • • • • ERTV • • • • EBCV • • • • EMAV1, MAV2 • • EReo Virus 1, 2 and 3 • EPneumonia virus of mice • ESendai virus • ESeoul virus • EParasites Pinworm – Syphacia obvelata, Aspiculuris tetraptera

• • • • E

Fur Mite – Myocoptes, Myobia and Radfordia spp.

• • • • E

Spironucleus muris • • • • EEntamoeba muris • • • • ETritrichomonas muris • • ECryptosporidium spp. • EGiardia spp. • E

Comprehensive GlobalBasicPrevelant

Rat Standard Profiles

Sample Type*

*Sample Type:

E = Environmental samples from open air flow design racks

• Specialized matrices or filters designed to capture exhaust air dust

• Flocked swab of rack exhaust air dust (prefilter, plenum)

• A sample of exhaust air dust (200 ul dust)

EDx Profiles

The science is right in our data

EDx Study Design

We’ve “racked our brains” so you don’t have to. We continue to provide the science behind the service. We’ve conducted comprehensive studies on multiple racks to evaluate the effectiveness of environmental diagnostics for detecting rodent pathogens in ventilated racks. Various racks were used in the studies including Allentown, Tecniplast, Animal Care System and Innovive. Based on the study data, we’ve designed our EDxPrime and EDxPlus services to provide customers with flexible options for developing an effective animal health monitoring program that fits your unique needs.

Mice housed 3/cage on ventilated racks

Infected mice

5-10 cages of mice infected with each of the

following organisms:

MNV

MHV

MPV

Helicobacter spp.

Pasteurella pneumotropica

Entamoeba muris

Tritrichomonas muris

Pinworms

Furmites

Uninfected mice

10 cages of uninfected mice

Animal Care Systems

Filtered: cage exhaust filter

Every 2 weeks

– Colony mice were monitored by

PCR (feces, fur swabs) to determine

if they were shedding the organisms.

– Dirty bedding transferred to sentinels

End of 12 weeks, testing included:

– Colony and sentinel Mice:

serology, PCR- feces & fur swab

– Environmental Diagnostics (EDx)

Innovive

– Colony mice were monitored by

PCR (feces, fur swabs) to determine

if they were shedding the organisms.

Similar actions as above, but includes:

– Swabs are taken at plenum junction

– Sentinels are on a separate rack

Filtered: exhaust filter from sentinel cages

Every 2 weeks

– Colony mice were monitored by

PCR (feces, fur swabs) to determine

if they were shedding the organisms.

– Dirty bedding transferred to sentinels

End of 12 weeks, testing included:

– Colony mice and sentinel mice:

serology, PCR- feces & fur swab

– Environmental Diagnostics (EDx)

Unfiltered airflow cage exhaust

Allentown–Sentinel™

Tecniplast–Interceptor

1. Unfiltered (no filter at exit)

* Tecniplast

* Allentown

2. Filtered (air exits via filter)

* Animal Care Systems

* Innovive

* Thoren

* Lab Products

There are two types of IVC Cage Exhaust:

Allentown Sentinel™

Infected mice Sentinel mice EDx–Sentinel™

Agent 0w 2w 4w 6w 8w 10w 12w 12w

MNV 10/10 10/10 10/10 10/10 10/10 10/10 8/10 6/10 +

MPV 4/5 4/5 4/5 4/5 4/5 4/5 4/5 9/10 +

MHV 5/5 5/5 1/5 0/5 0/5 0/5 0/5 7/10 +

Helicobacter spp. 5/5 5/5 5/5 5/5 5/5 5/5 5/5 0/10 +

P. pneumotropica 5/5 5/5 5/5 5/5 5/5 5/5 5/5 0/10 +

E. muris 5/5 5/5 5/5 4/5 5/5 5/5 5/5 9/10 +

T. muris 0/5 1/5 1/5 1/5 1/5 1/5 1/5 0/10 –

Pinworms 5/5 5/5 5/5 5/5 5/5 5/5 5/5 5/10 +

Furmites 5/5 5/5 5/5 5/5 5/5 5/5 5/5 0/10 +

Allentown SentinelTM

• EDx testing of SentinelTM

detected pathogens missed by dirty bedding sentinel animals

• EDx testing is a valuable tool to include in your AHM program

Tecniplast: Interceptor

Infected mice Sentinel mice EDx–Interceptor

Agent 0w 2w 4w 6w 8w 10w 12w 12w

MNV 10/10 10/10 10/10 10/10 10/10 10/10 8/10 10/10 +

MPV 5/5 5/5 5/5 5/5 5/5 5/5 5/5 10/10 +

MHV 5/5 5/5 1/5 0/5 0/5 0/5 0/5 4/10 +

Helicobacter spp. 5/5 5/5 5/5 5/5 5/5 5/5 5/5 3/10 +

P. pneumotropica 5/5 5/5 5/5 5/5 5/5 5/5 5/5 0/10 +

E. muris 5/5 5/5 5/5 4/5 5/5 5/5 5/5 9/10 +

T. muris 5/5 5/5 5/5 5/5 4/5 5/5 5/5 0/10 –

Pinworms 5/5 5/5 5/5 5/5 5/5 5/5 4/5 6/10 +

Furmites 5/5 5/5 5/5 5/5 5/5 5/5 5/5 0/10 +

Tecniplast Interceptor

• EDx testing of Interceptor detected pathogens missed by dirty bedding sentinel animals

• EDx testing is a valuable tool to include in your AHM program

Animal Care Systems | Cage Exhaust Filter

Infected mice Sentinel mice

EDx–pooled1

EDx– 12 wks2

Agent 0w 2w 4w 6w 8w 10w 12w Filter Filter

MNV 8/10 7/10 7/10 7/10 7/10 7/10 3/10 10/12 3/3 1/3

MPV 5/5 5/5 5/5 5/5 4/5 5/5 4/5 12/12 3/3 3/3

MHV 5/5 5/5 2/5 0/5 0/5 0/5 0/5 6/12 1/3 0/3

Helicobacter spp. 5/5 5/5 5/5 5/5 5/5 5/5 5/5 0/12 3/3 3/3

P. pneumotropica 5/5 5/5 5/5 5/5 5/5 5/5 5/5 0/12 2/3 2/3

E. muris 5/5 5/5 5/5 5/5 5/5 5/5 5/5 10/12 3/3 3/3

T. muris 3/5 2/5 3/5 3/5 3/5 3/5 2/5 0/12 0/3 0/3

Pinworms 5/5 5/5 5/5 5/5 5/5 5/5 5/5 5/12 3/3 3/3

Furmites 5/5 5/5 5/5 5/5 5/5 5/5 5/5 0/12 2/3 2/31 Filter collected every 2 weeks and pooled. 2 Filter moved with sentinel cage and tested at the end of 12 weeks.

Innovive | Plenum Swab

Infected mice EDx–Plenum swab

Agent 0w 1w 2w 3w 4w 12w 12w

MNV 5/5 5/5 5/5 5/5 5/5 2/5 –

MPV 5/5 5/5 5/5 5/5 5/5 5/5 –

MHV 5/5 5/5 5/5 4/5 3/5 0/5 –

Helicobacter spp. 5/5 5/5 5/5 5/5 5/5 5/5 –

P. pneumotropica 5/5 5/5 5/5 5/5 5/5 5/5 –

E. muris 4/5 5/5 5/5 5/5 5/5 5/5 –

T. muris 4/5 5/5 5/5 4/5 5/5 5/5 –

Pinworms 5/5 5/5 5/5 5/5 5/5 5/5 –

Furmites 5/5 5/5 5/5 5/5 5/5 5/5 –

Animal Care Systems

• EDx testing detected pathogens missed by dirty bedding sentinel testing

• EDx testing is a valuable tool to include in your AHM program

Innovive

• EDx testing of plenums did not detect pathogens

• Type of rack and design of rack is important when implementing EDx monitoring programs

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To explore EDx options for your facility, contact IDEXX BioAnalytics: 1 800 669 0825 • idexxbioanalytics.com

EDx • 3R’sEnvironmental monitoring can play a key role while allowing you to Replace the use of animals with alternative testing techniques, Reduce the number of animals used to a minimum, and Refine the way health monitoring is done. IDEXX BioAnalytics Environmental Diagnostics support the 3R’s while providing high-quality research lab animal testing services so you’ll get results you can trust.