Education and Career Development Workshop

ASV – 2011 MinneapolisASV – 2011 Minneapolis

Christopher C. BroderChristopher C. BroderUniformed Services University, Bethesda, MDUniformed Services University, Bethesda, MD

My Science Career -- How do I Keep Moving Forward?My Science Career -- How do I Keep Moving Forward?

Streptococcus pyogenes surface proteins as Streptococcus pyogenes surface proteins as receptors for human plasmin.receptors for human plasmin.

Ph.D. Thesis (89’), University of Florida, Gainesville

Vincent A. Fischetti

Grewe & GeldeblomGrewe & Geldeblom

HIV Entry HIV Entry (1989)(1989)

HIV EntryHIV Entry

Dimiter (Mitko) S. Dimitrov, Dimiter (Mitko) S. Dimitrov, Sc.D.,Ph.D.Sc.D.,Ph.D.NCI-Frederick, Frederick, MD NCI-Frederick, Frederick, MD

HIV Tropism HIV Tropism

Broder, C.C., D.S. Dimitrov, R. Blumenthal, and E.A. Berger. The Block to HIV-1 Envelope Glycoprotein-Mediated Membrane Fusion in Animal Cells Expressing Human CD4 can be Overcome by a Human Cell Component(s). Virology. 193:483-491, 1993.

Think of new Think of new approaches and approaches and

seek new seek new collaborationscollaborations

CXCR4 CCR5

Sir Francis BaconSir Francis Bacon English author, courtier, & philosopher (1561 - 1626) ...English author, courtier, & philosopher (1561 - 1626) ...

““A wise man will make A wise man will make more opportunities than he more opportunities than he finds” finds”

Oligomeric HIV-1 Envelope (gp140)---Oligomeric HIV-1 Envelope (gp140)---

--with Pat Earl and Bob Doms (LVD, NIAID)--with Pat Earl and Bob Doms (LVD, NIAID)

Broder, C.C., P.L. Earl, et al., Proc. Natl. Acad. Sci. USA. 91:11699-11703, 1994.Earl, P.L., C.C. Broder, et al., J. Virol. 68: 3015-3026, 1994.

Seek out new collaborations------ it works!Seek out new collaborations------ it works!

Transition from postdoctoral position to academia---Transition from postdoctoral position to academia---(Assistant professor -1996, Uniformed Services University)

Start off with the most active and Start off with the most active and successful part of your research successful part of your research activities-activities-

HIV tropism and entry……

HIV-1 Fusion CofactorsR01 - NIAID

Analysis of Oligomeric HIV-1 Envelope Glycoproteins

R21 - NIAID HIV-1 Envelope-CD4-Coreceptor

Complexes as Vaccines R21 - NIAID

Broadly Effective Neutralization and CTL against HIV-1

P01 - NIAIDHIV-1 Fusion Cofactors

R29 – NIAID, 96’ High Potency HIV-1 Broadly Cross-Reactive Neutralization

U01 – NIAID, 08’

This worked: (Associate Professor - 2000, Uniformed Services University)

How about trying something How about trying something “completely-“completely-different”different” as part of your research as part of your research activities-activities-

How to make that next transition to Professor?How to make that next transition to Professor?

Now what?Now what?

Reinvent some of your research endeavors….Reinvent some of your research endeavors….

Seek out new collaborations------ it works!Seek out new collaborations------ it works!

Lin-Fa Wang, Ph.D.Lin-Fa Wang, Ph.D.CSIRO, Australian Animal Health CSIRO, Australian Animal Health Laboratory, Geelong, AustraliaLaboratory, Geelong, Australia

Hendra virus 1999

““Small opportunities are often the Small opportunities are often the beginning of great enterprises”beginning of great enterprises”

Demosthenes (384 BC - 322 BC)Demosthenes (384 BC - 322 BC)

Nipah/Hendra Subunit Vaccines R21 - NIAID

Biodefense and Emerging Infectious Diseases Research (Nipah/Hendra)

U54-(RCE) - NIAID Vaccines/Therapeutics for

Nipah/Hendra U01 - NIAID

Nipah/Hendra Virus Entry and Assembly

R01 - NIAID

Nipah/Hendra Nonhuman Primate Models Therapeutics Development

U01 – NIAID

Biodefense and Emerging Infectious Diseases Research (bat lyssavirus)

U54-(RCE) - NIAID

New grounds….New grounds….

This worked as well: (Professor - 2005, Uniformed Services University)

Nipah/Hendra Peptide TherapeuticsU01 – NIAID, 2003

Katharine Bossart, Ph.D.

Human monoclonal antibody protection Human monoclonal antibody protection against Hendra virus in NHPs, ASV-2011, against Hendra virus in NHPs, ASV-2011,

40-140-1

Seek out new collaborations------ it still works!Seek out new collaborations------ it still works!Keep moving forwardKeep moving forward

Nonhuman Primate Models

for Henipaviruses

Thomas Geisbert

Australian Bat Lyssavirus(ABLV)

Structural Biology Program Memorial Sloan-Kettering Cancer Center, New York Dimitar B. Nikolov and Kai Xu

Linfa WangLinfa WangIna SmithIna Smith

A couple of take home messages---- A couple of take home messages----

What has worked for me……….What has worked for me……….

#1 -- Seek out new collaborations-- it usually #1 -- Seek out new collaborations-- it usually works!works!

A couple of take home messages---- A couple of take home messages----

What has worked for me………. What has worked for me……….

#2 --About every 5 years --- #2 --About every 5 years ---

Reinvent at least a third of your research program…Reinvent at least a third of your research program…

Doing so can help you stay energized ---- but also…..Doing so can help you stay energized ---- but also…..

Attracts new students and young scientists with different interests and talents --- adding depth to your team.

Gives you new or different research Gives you new or different research funding opportunities to pursue.funding opportunities to pursue.

Periodically reinvent your program……

Often, to achieve success in doing this, those new research directions will be related to your current productive programs

Studies on henipavirus - entryStudies on henipavirus - entry

An excellent example…..An excellent example…..

DiagnosticsDiagnostics

Sero-surveillanceSero-surveillance

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Immunotherapy Immunotherapy Human mAbsHuman mAbs

Viral membrane glycoproteinsViral membrane glycoproteins

Hendra Hendra sGsG

VaccinVaccineses

Hendra sG

A couple of take home messages---- A couple of take home messages----

What has worked for me……….What has worked for me……….

#3 -- Always look down the road about 2 to 3 #3 -- Always look down the road about 2 to 3 years…. years….

Keep an eye out for new opportunities and Keep an eye out for new opportunities and always always be aware of the limits of your be aware of the limits of your resources.resources.

Dawn Weir USU/EIDDawn Weir USU/EIDAustralian bat lyssavirus – tropismAustralian bat lyssavirus – tropism

ASV-2011, P1-14ASV-2011, P1-14

Stephanie Petzing USU/EIDStephanie Petzing USU/EIDBat derived cells and emerging viruses Bat derived cells and emerging viruses

ASV-2011, W39-3ASV-2011, W39-3

Andrew Hickey, MPH, Ph.D.Andrew Hickey, MPH, Ph.D.sG-vaccination in NHPssG-vaccination in NHPs

ASV-2011, W40-2ASV-2011, W40-2

Deborah Steffen – USU/EIDDeborah Steffen – USU/EIDNext-Generation human mabs against Next-Generation human mabs against

henipaviruseshenipavirusesASV-2011, W42-8ASV-2011, W42-8

Be aware of the limits of your resources----Be aware of the limits of your resources---- to keep your lab and staff to keep your lab and staff supported- especially those students that have committed themselves to your supported- especially those students that have committed themselves to your laboratory.laboratory.

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